1992
Human Hybridoma Generation by Hypo-Osmolar Electrofusion: Characterization of Human Monoclonal Antibodies to Schistosoma mansoni Parasite Antigens
Klock G, Wisnewski AV, el-Bassiouni EA, Ramadan MI, Gessner P, Zimmermann U, Kresina TF. Human Hybridoma Generation by Hypo-Osmolar Electrofusion: Characterization of Human Monoclonal Antibodies to Schistosoma mansoni Parasite Antigens. Monoclonal Antibodies In Immunodiagnosis And Immunotherapy 1992, 11: 469-481. PMID: 1398684, DOI: 10.1089/hyb.1992.11.469.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyMonoclonal antibodiesIgG antibodiesParasite antigensHypo-osmolar electrofusionS. mansoniSchistosoma mansoni wormsCell linesWestern blot analysisEgg antigenParasitic antigensAntigen reactivityAntigenAntibodiesSplenocytesBlot analysisHybridomasCandidate moleculesMansoniHybridoma generationMitogenCell fusionSomatic cell fusionGreater numberELISA
1991
Screening of murine monoclonal antibodies against living schistosomula of Schistosoma mansoni by radioimmunoassay
Wiest P, Wisnewski A, Johnson J, Ramirez B, Kresina T, Olds G. Screening of murine monoclonal antibodies against living schistosomula of Schistosoma mansoni by radioimmunoassay. International Journal For Parasitology 1991, 21: 449-454. PMID: 1917285, DOI: 10.1016/0020-7519(91)90102-d.Peer-Reviewed Original ResearchConceptsMurine monoclonal antibodiesSurface antigenSoluble adult worm antigenSchistosoma mansoniMonoclonal antibodiesS. mansoni paramyosinS. mansoni infectionSoluble egg antigenAdult worm antigenEnzyme-linked immunosorbentMansoni infectionEgg antigenLung stageWorm antigenS. mansoniSecrete antibodiesAntigenSchistosomulaAdult wormsAntibodiesRadioimmunoassayMansoniImmunoblot analysisOld parasitesMurine hybridomas