2021
Single-cell longitudinal analysis of SARS-CoV-2 infection in human airway epithelium identifies target cells, alterations in gene expression, and cell state changes
Ravindra NG, Alfajaro MM, Gasque V, Huston NC, Wan H, Szigeti-Buck K, Yasumoto Y, Greaney AM, Habet V, Chow RD, Chen JS, Wei J, Filler RB, Wang B, Wang G, Niklason LE, Montgomery RR, Eisenbarth SC, Chen S, Williams A, Iwasaki A, Horvath TL, Foxman EF, Pierce RW, Pyle AM, van Dijk D, Wilen CB. Single-cell longitudinal analysis of SARS-CoV-2 infection in human airway epithelium identifies target cells, alterations in gene expression, and cell state changes. PLOS Biology 2021, 19: e3001143. PMID: 33730024, PMCID: PMC8007021, DOI: 10.1371/journal.pbio.3001143.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectionSARS-CoV-2Human bronchial epithelial cellsInterferon-stimulated genesCell state changesAcute respiratory syndrome coronavirus 2 infectionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infectionSyndrome coronavirus 2 infectionCell tropismCoronavirus 2 infectionCoronavirus disease 2019Onset of infectionCell-intrinsic expressionCourse of infectionAir-liquid interface culturesHost-viral interactionsBronchial epithelial cellsSingle-cell RNA sequencingCell typesIL-1Disease 2019Human airwaysDevelopment of therapeuticsDrug AdministrationViral replication
2020
SARS-CoV-2 infection of the placenta
Hosier H, Farhadian SF, Morotti RA, Deshmukh U, Lu-Culligan A, Campbell KH, Yasumoto Y, Vogels C, Casanovas-Massana A, Vijayakumar P, Geng B, Odio CD, Fournier J, Brito AF, Fauver JR, Liu F, Alpert T, Tal R, Szigeti-Buck K, Perincheri S, Larsen C, Gariepy AM, Aguilar G, Fardelmann KL, Harigopal M, Taylor HS, Pettker CM, Wyllie AL, Dela Cruz CS, Ring AM, Grubaugh ND, Ko AI, Horvath TL, Iwasaki A, Reddy UM, Lipkind HS. SARS-CoV-2 infection of the placenta. Journal Of Clinical Investigation 2020, 130: 4947-4953. PMID: 32573498, PMCID: PMC7456249, DOI: 10.1172/jci139569.Peer-Reviewed Case Reports and Technical NotesMeSH KeywordsAbortion, TherapeuticAbruptio PlacentaeAdultBetacoronavirusCoronavirus InfectionsCOVID-19FemaleHumansMicroscopy, Electron, TransmissionPandemicsPhylogenyPlacentaPneumonia, ViralPre-EclampsiaPregnancyPregnancy Complications, InfectiousPregnancy Trimester, SecondRNA, ViralSARS-CoV-2Viral LoadConceptsSevere acute respiratory syndrome coronavirus 2Acute respiratory syndrome coronavirus 2SARS-CoV-2 infectionRespiratory syndrome coronavirus 2SARS-CoV-2 invasionMaternal antibody responseSymptomatic COVID-19Second trimester pregnancySyndrome coronavirus 2Coronavirus disease 2019Materno-fetal interfaceDense macrophage infiltratesPlacental abruptionSevere preeclampsiaMacrophage infiltratesSevere morbidityTrimester pregnancyPregnant womenCoronavirus 2Antibody responseBackgroundThe effectsDisease 2019Histological examinationImmunohistochemical assaysPlacenta
2019
Mediation of the Acute Stress Response by the Skeleton
Berger JM, Singh P, Khrimian L, Morgan DA, Chowdhury S, Arteaga-Solis E, Horvath TL, Domingos AI, Marsland AL, Yadav V, Rahmouni K, Gao XB, Karsenty G. Mediation of the Acute Stress Response by the Skeleton. Cell Metabolism 2019, 30: 890-902.e8. PMID: 31523009, PMCID: PMC6834912, DOI: 10.1016/j.cmet.2019.08.012.Peer-Reviewed Original ResearchConceptsStress responseBony vertebratesAcute stress responseBone-derived signalsWild-type animalsGenetic studiesEndocrine mediationAdrenal insufficient patientsVertebratesOsteocalcinSympathetic toneParasympathetic neuronsWildOsteocalcin levelsStressorsTypes of stressorsSelective surgeOsteoblastsInactivationRodentsResponseGlutamateUptakeMitofusin 2 in Mature Adipocytes Controls Adiposity and Body Weight
Mancini G, Pirruccio K, Yang X, Blücher M, Rodeheffer M, Horvath TL. Mitofusin 2 in Mature Adipocytes Controls Adiposity and Body Weight. Cell Reports 2019, 26: 2849-2858.e4. PMID: 30865877, PMCID: PMC6876693, DOI: 10.1016/j.celrep.2019.02.039.Peer-Reviewed Original ResearchConceptsKnockout miceBody weightMitochondria-endoplasmic reticulum interactionSystemic metabolic dysregulationImpaired glucose metabolismHigh-fat dietObese human subjectsCalorie-dense foodsMitofusin 2Control miceStandard chowLean controlsMetabolic dysregulationFood intakeAdult miceGlucose metabolismStandard dietAdipose tissueBrown fatGlucose utilizationAdiposityTissue levelsSystemic levelsMiceAdult animals
2018
Aromatase and estrogen receptor immunoreactivity in the coronary arteries of monkeys and human subjects
Diano S, Horvath TL, Mor G, Register T, Adams M, Harada N, Naftolin F. Aromatase and estrogen receptor immunoreactivity in the coronary arteries of monkeys and human subjects. Menopause The Journal Of The North American Menopause Society 2018, 25: 1201-1207. PMID: 30358714, DOI: 10.1097/gme.0000000000001219.Peer-Reviewed Original ResearchConceptsCoronary arteryEstrogen receptor immunoreactivityAmount of atherosclerosisHuman placental estrogen synthetaseHuman subjectsSmooth muscle cellsPrecursor androgensReceptor immunoreactivityCardioprotective effectsCoronary circulationEstrogen formationEstrogen receptorArteryCardiovascular systemER αMuscle cellsEndothelial cellsEstrogenEstrogen synthetaseMonkeysFirst evidenceSubjectsCellsAtherosclerosisLocal regulation
2017
Ghrelin is Related to Personality Differences in Reward Sensitivity and Impulsivity
Ralevski E, Shanabrough M, Newcomb J, Gandelman E, Hayden R, Horvath TL, Petrakis I. Ghrelin is Related to Personality Differences in Reward Sensitivity and Impulsivity. Alcohol And Alcoholism 2017, 53: 52-56. PMID: 29136100, DOI: 10.1093/alcalc/agx082.Peer-Reviewed Original ResearchConceptsAspects of impulsivityTraits of impulsivityHealthy social drinkersSocial drinkersReward sensitivityImpulsivity traitsPersonality measuresPersonality differencesImpulsivityDrugs of abusePersonality characteristicsSubjective effectsDirect relationshipHigh levelsDrinkersFeeding-related peptidesPunishmentFirst studyIndividualsTraitsSecondary analysisParticipantsAbuseRelationshipOriginal studyGhrelin is Supressed by Intravenous Alcohol and is Related to Stimulant and Sedative Effects of Alcohol
Ralevski E, Horvath TL, Shanabrough M, Hayden R, Newcomb J, Petrakis I. Ghrelin is Supressed by Intravenous Alcohol and is Related to Stimulant and Sedative Effects of Alcohol. Alcohol And Alcoholism 2017, 52: 431-438. PMID: 28481974, DOI: 10.1093/alcalc/agx022.Peer-Reviewed Original ResearchConceptsGhrelin levelsDoses of alcoholHealthy social drinkersSubjective effectsTG levelsAlcohol infusionSedative effectsLow doseHigh doseOral alcohol administrationIntravenous alcohol infusionFeeding-related peptidesSocial drinkersAlcohol administrationIntravenous alcoholOral alcoholGhrelinPercent changeInfusionRewarding propertiesBehavioral effectsAlcohol effectsDoseTime pointsSignificant predictors
2015
The ketone metabolite β-hydroxybutyrate blocks NLRP3 inflammasome–mediated inflammatory disease
Youm YH, Nguyen KY, Grant RW, Goldberg EL, Bodogai M, Kim D, D'Agostino D, Planavsky N, Lupfer C, Kanneganti TD, Kang S, Horvath TL, Fahmy TM, Crawford PA, Biragyn A, Alnemri E, Dixit VD. The ketone metabolite β-hydroxybutyrate blocks NLRP3 inflammasome–mediated inflammatory disease. Nature Medicine 2015, 21: 263-269. PMID: 25686106, PMCID: PMC4352123, DOI: 10.1038/nm.3804.Peer-Reviewed Original Research
2013
Natural birth-induced UCP2 in brain development
Seli E, Horvath TL. Natural birth-induced UCP2 in brain development. Reviews In Endocrine And Metabolic Disorders 2013, 14: 347-350. PMID: 23979530, DOI: 10.1007/s11154-013-9262-8.Peer-Reviewed Original ResearchConceptsVaginal birthPost-operative surgical careLong-term outcomesEarly postnatal periodWild-type littermatesAdult brain structureUCP2 mRNA expressionTerm outcomesSurgical techniqueSurgical carePostnatal periodSurgical meansHippocampal formationMore deliveriesSynapse formationAxonal outgrowthC-sectionKnockout animalsMRNA expressionUCP2 expressionBrain developmentBrain structuresNeuronal differentiationBirthHippocampus
2011
Obesity is associated with hypothalamic injury in rodents and humans
Thaler JP, Yi CX, Schur EA, Guyenet SJ, Hwang BH, Dietrich MO, Zhao X, Sarruf DA, Izgur V, Maravilla KR, Nguyen HT, Fischer JD, Matsen ME, Wisse BE, Morton GJ, Horvath TL, Baskin DG, Tschöp MH, Schwartz MW. Obesity is associated with hypothalamic injury in rodents and humans. Journal Of Clinical Investigation 2011, 122: 153-162. PMID: 22201683, PMCID: PMC3248304, DOI: 10.1172/jci59660.Peer-Reviewed Original ResearchConceptsHigh-fat dietHFD feedingMediobasal hypothalamusPeripheral tissuesRodent modelsBody weight controlHypothalamic arcuate nucleusSubstantial weight gainConsequences of obesityNeuron injuryHypothalamic injuryNeuronal injuryNeuroprotective mechanismsReactive gliosisObese humansHypothalamic areaArcuate nucleusInflammatory signalingBrain areasWeight controlObesityGliosisEnergy homeostasisWeight gainInflammationGhrelin Enhances Olfactory Sensitivity and Exploratory Sniffing in Rodents and Humans
Tong J, Mannea E, Aimé P, Pfluger PT, Yi CX, Castaneda TR, Davis HW, Ren X, Pixley S, Benoit S, Julliard K, Woods SC, Horvath TL, Sleeman MM, D'Alessio D, Obici S, Frank R, Tschöp MH. Ghrelin Enhances Olfactory Sensitivity and Exploratory Sniffing in Rodents and Humans. Journal Of Neuroscience 2011, 31: 5841-5846. PMID: 21490225, PMCID: PMC3089941, DOI: 10.1523/jneurosci.5680-10.2011.Peer-Reviewed Original ResearchConceptsExploratory sniffingAppetite-stimulating hormone ghrelinOlfactory sensitivityOverall functionGhrelin infusionIntracerebroventricular ghrelinGhrelin receptorHormone ghrelinSaline infusionOlfactory functionNeuroendocrine circuitsGhrelinUnderlying neural mechanismsEnergy homeostasisOdor detectionOlfactory processingOlfactory circuitFood seekingNeural mechanismsSniff magnitudeInfusionNovel roleOlfactory detectionSniffingSpecific effectsGhrelin-induced hypothermia: A physiological basis but no clinical risk
Wiedmer P, Strasser F, Horvath TL, Blum D, DiMarchi R, Lutz T, Schürmann A, Joost HG, Tschöp MH, Tong J. Ghrelin-induced hypothermia: A physiological basis but no clinical risk. Physiology & Behavior 2011, 105: 43-51. PMID: 21513721, PMCID: PMC3146973, DOI: 10.1016/j.physbeh.2011.03.027.Peer-Reviewed Original ResearchConceptsGhrelin treatmentBody temperatureApplication of ghrelinMedial preoptic areaPotential anatomical basisCold-sensitive neuronsGhrelin infusionBody core temperatureChronic i.Positive energy balanceGhrelin receptorPreoptic areaAxon terminalsClinical riskFood intakeGhrelinHealthy humansSerious hypothermiaMale subjectsPhysiologic circumstancesAnatomical basisHypothermiaCold exposureRelevant decreaseEnergy expenditure
1996
Aromatase Immunoreactivity in Axon Terminals of the Vertebrate Brain
Naftolin F, Horvath T, Jakab R, Leranth C, Harada N, Balthazart J. Aromatase Immunoreactivity in Axon Terminals of the Vertebrate Brain. Neuroendocrinology 1996, 63: 149-155. PMID: 9053779, DOI: 10.1159/000126951.Peer-Reviewed Original ResearchConceptsAxon terminalsAromatase immunoreactivityAxonal processesDifferent vertebrate speciesAdult central nervous systemRole of aromataseSmall clear synaptic vesiclesCentral nervous systemClear synaptic vesiclesVertebrate speciesSubcellular locationMost vertebratesSpecific limbicNeuronal perikaryaAromatase activityElectron microscopic examinationEstrogen synthesisHypothalamic structuresSubcellular distributionSynaptic levelVertebrate brainNervous systemBrain aromataseMolecular biologyIntraneuronal production