2021
Salt-Inducible Kinase 3 Promotes Vascular Smooth Muscle Cell Proliferation and Arterial Restenosis by Regulating AKT and PKA-CREB Signaling
Cai Y, Wang XL, Lu J, Lin X, Dong J, Guzman RJ. Salt-Inducible Kinase 3 Promotes Vascular Smooth Muscle Cell Proliferation and Arterial Restenosis by Regulating AKT and PKA-CREB Signaling. Arteriosclerosis Thrombosis And Vascular Biology 2021, 41: 2431-2451. PMID: 34196217, PMCID: PMC8411910, DOI: 10.1161/atvbaha.121.316219.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MovementCell ProliferationCells, CulturedConstriction, PathologicCREB-Binding ProteinCyclic AMP-Dependent Protein KinasesCyclin-Dependent Kinase Inhibitor p21Cyclin-Dependent Kinase Inhibitor p27Disease Models, AnimalFemaleFemoral ArteryMaleMice, Inbred C57BLMuscle, Smooth, VascularMyocytes, Smooth MuscleNeointimaPhenylurea CompoundsProtein Kinase InhibitorsProtein Serine-Threonine KinasesProto-Oncogene Proteins c-aktPyrimidinesRats, Sprague-DawleySignal TransductionTranscription FactorsVascular System InjuriesConceptsSmooth muscle cellsArterial restenosisNeointima formationSMC proliferationSMC migrationSIK inhibitionVascular smooth muscle cell proliferationPeripheral arterial diseaseSmooth muscle cell proliferationVascular smooth muscle cellsAortic smooth muscle cellsPotential therapeutic strategyMuscle cell proliferation
2019
Medial artery calcification increases neointimal hyperplasia after balloon injury
Marshall AP, Luo W, Wang XL, Lin T, Cai Y, Guzman RJ. Medial artery calcification increases neointimal hyperplasia after balloon injury. Scientific Reports 2019, 9: 8193. PMID: 31160618, PMCID: PMC6547750, DOI: 10.1038/s41598-019-44668-4.Peer-Reviewed Original ResearchMeSH KeywordsAngioplasty, BalloonAnimalsBone Morphogenetic Protein 2CalcinosisCalcium ChlorideCarotid ArteriesCarotid Artery InjuriesCell ProliferationCoronary RestenosisDisease Models, AnimalHyperplasiaMaleMuscle, Smooth, VascularMyocytes, Smooth MuscleNeointimaProliferating Cell Nuclear AntigenRatsRats, Sprague-DawleyTunica IntimaConceptsSmooth muscle cellsNeointimal hyperplasiaBalloon injuryArterial calcificationCalcified arteriesMedial smooth muscle cellsCell nuclear antigen stainingBalloon catheter injuryMedial artery calcificationTime of injuryVascular smooth muscle cellsNeointimal cell proliferationCell proliferationCatheter injuryArtery calcificationMedial calcificationSubcutaneous injectionAntigen stainingVitamin D3Rat carotidEffect of calcificationArteryHyperplasiaAdventitial applicationInjury
2018
Inhibition of endo-lysosomal function exacerbates vascular calcification
Cai Y, Wang XL, Flores AM, Lin T, Guzman RJ. Inhibition of endo-lysosomal function exacerbates vascular calcification. Scientific Reports 2018, 8: 3377. PMID: 29467541, PMCID: PMC5821871, DOI: 10.1038/s41598-017-17540-6.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsVascular calcificationAortic rings ex vivoChronic kidney diseaseMedial artery calcificationSmooth muscle cellsLysosomal inhibitor chloroquineCardiovascular morbidityArtery calcificationPathologic responseDiabetes mellitusAortic ringsKidney diseaseMedial calcificationVitamin D3Osteogenic transformationVSMC calcificationLysosomal marker lysosomeProtective roleLysosome inhibitionInhibitor chloroquineMembrane protein 2Ex vivoMuscle cellsCalcification
2016
Dorsomorphin homologue 1, a highly selective small-molecule bone morphogenetic protein inhibitor, suppresses medial artery calcification
Lin T, Wang XL, Zettervall SL, Cai Y, Guzman RJ. Dorsomorphin homologue 1, a highly selective small-molecule bone morphogenetic protein inhibitor, suppresses medial artery calcification. Journal Of Vascular Surgery 2016, 66: 586-593. PMID: 27374065, PMCID: PMC5201454, DOI: 10.1016/j.jvs.2016.03.462.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicAortic DiseasesBone Morphogenetic Protein Receptors, Type IBone Morphogenetic ProteinsCells, CulturedHumansMuscle, Smooth, VascularMyocytes, Smooth MuscleOrgan Culture TechniquesOsteogenesisPhosphatesPyrazolesQuinolinesRats, Sprague-DawleySignal TransductionVascular CalcificationConceptsMedial artery calcificationSmooth muscle cellsArtery calcificationMedial calcificationOrgan culture modelVascular patientsVascular calcificationWestern blotCalcium accumulationHuman aortic smooth muscle cellsChronic kidney diseaseCulture modelVascular smooth muscle cellsAortic smooth muscle cellsQuantitative reverse transcription polymerase chain reactionReverse transcription-polymerase chain reactionSMC markersTranscription-polymerase chain reactionHuman smooth muscle cellsAortic medial calcificationMuscle myosin heavy chainHomologue 1Bone morphogenetic proteinKidney diseasePolymerase chain reaction