2016
CJD and Scrapie Require Agent‐Associated Nucleic Acids for Infection
Botsios S, Manuelidis L. CJD and Scrapie Require Agent‐Associated Nucleic Acids for Infection. Journal Of Cellular Biochemistry 2016, 117: 1947-1958. PMID: 26773845, DOI: 10.1002/jcb.25495.Peer-Reviewed Original ResearchConceptsTSE agentsTransmissible spongiform encephalopathiesAdaptive immune responsesDegenerative brain changesInfectious particlesHost prion proteinGT1 neuronal cellsForms of PrPLymphoreticular tissuesBrain changesImmune responseTSE strainsNeuronal cellsNeurodegenerative diseasesLatent virusInfectivity assaysSpongiform encephalopathiesNucleic acid genomeTitersEpidemic spreadViral structuresPrion proteinHost proteinsVirusHost components
2015
Rapid chemical decontamination of infectious CJD and scrapie particles parallels treatments known to disrupt microbes and biofilms
Botsios S, Tittman S, Manuelidis L. Rapid chemical decontamination of infectious CJD and scrapie particles parallels treatments known to disrupt microbes and biofilms. Virulence 2015, 6: 787-801. PMID: 26556670, PMCID: PMC4826107, DOI: 10.1080/21505594.2015.1098804.Peer-Reviewed Original ResearchConceptsInfectious titerInnate immune responseHuman CJDResistant virusesIatrogenic infectionGT1 cellsBrain changesImmune responseInfectious agentsTSE agentsScrapie agentCJDPrion protein amyloidSheep scrapieVirulent microbesInfectious particlesMin exposureIntrinsic resistanceTreatmentTitersPrP amyloidScrapieAmyloidCultured cellsDeep proteomic analysis
2013
Infectious particles, stress, and induced prion amyloids
Manuelidis L. Infectious particles, stress, and induced prion amyloids. Virulence 2013, 4: 373-383. PMID: 23633671, PMCID: PMC3714129, DOI: 10.4161/viru.24838.Peer-Reviewed Original ResearchConceptsDiverse organismsInfectious TSE agentEnvironmental metagenomesTransmissible encephalopathiesHost prion proteinProtein misfoldingInfectious amyloidProtein modelingSusceptible PrP genotypesPrion amyloidInnate immune responsePrion proteinNeuronal agingDifferent amyloid proteinsTSE agentsMicrobial agentsNeurodegenerative diseasesPublic health measuresOrganismsBiological characteristicsNucleic acidsInfectious particlesAmyloid proteinProteinEndemic scrapie
2011
Replication and spread of CJD, kuru and scrapie agents in vivo and in cell culture
Miyazawa K, Emmerling K, Manuelidis L. Replication and spread of CJD, kuru and scrapie agents in vivo and in cell culture. Virulence 2011, 2: 188-199. PMID: 21527829, PMCID: PMC3149681, DOI: 10.4161/viru.2.3.15880.Peer-Reviewed Original ResearchConceptsGT1 cellsSporadic CJDTSE agentsScrapie agentAgent-specific patternsTransmissible spongiform encephalopathy agentsComplex innate immune responseSpongiform encephalopathy agentInnate immune responseHost prion proteinK scrapie agentHuman CJDCJD agentNeuropathological sequelaeBrain titersImmune responseHuman kuruClearance mechanismsCJDInhibitory effectEnvironmental agentsCell-based assaysKuruInfectious formDistinct incubation times
2010
Nuclease resistant circular DNAs copurify with infectivity in scrapie and CJD
Manuelidis L. Nuclease resistant circular DNAs copurify with infectivity in scrapie and CJD. Journal Of NeuroVirology 2010, 17: 131-145. PMID: 21165784, DOI: 10.1007/s13365-010-0007-0.Peer-Reviewed Original ResearchMeSH KeywordsAcinetobacterAmino Acid SequenceAnimalsBase SequenceBrainConserved SequenceCreutzfeldt-Jakob SyndromeCricetinaeDeoxyribonucleasesDNA HelicasesDNA, CircularDNA, MitochondrialDNA-Directed DNA PolymeraseElectrophoresis, Agar GelGenome, BacterialHumansMiceMolecular Sequence DataNeuroblastomaNeurodegenerative DiseasesPlasmidsPolymerase Chain ReactionPrionsScrapieTumor Cells, CulturedTransmissible encephalopathy agents
Manuelidis L. Transmissible encephalopathy agents. Virulence 2010, 1: 101-104. PMID: 21178425, PMCID: PMC3073180, DOI: 10.4161/viru.1.2.10822.Peer-Reviewed Original ResearchConceptsTransmissible spongiform encephalopathiesKuru agentNormal miceRecent transmissionInfectious agentsTissue pathologyTSE agentsDisease latencyVirulence characteristicsNeural cellsSpongiform encephalopathiesPrion hypothesisEpidemic spreadAgentsGeographic regionsEncephalopathyVirulenceSuperinfectionMicePathologyAgent-specific Shadoo Responses in Transmissible Encephalopathies
Miyazawa K, Manuelidis L. Agent-specific Shadoo Responses in Transmissible Encephalopathies. Journal Of Neuroimmune Pharmacology 2010, 5: 155-163. PMID: 20112073, PMCID: PMC2875883, DOI: 10.1007/s11481-010-9191-1.Peer-Reviewed Original ResearchConceptsAgent-specific differencesTransmissible spongiform encephalopathiesGT1 cellsTga20 miceTSE agentsHost prion proteinKuru agentRegional neuropathologyIntracellular amyloidSporadic CJDPrP changesTSE infectionUninfected controlsPrP levelsInfectious agentsAbnormal PrPTransmissible encephalopathiesMiceNeurodegenerative diseasesScrapie agentCJDInfectionInfected cellsSheep scrapieSpongiform encephalopathies
2009
The kuru infectious agent is a unique geographic isolate distinct from Creutzfeldt–Jakob disease and scrapie agents
Manuelidis L, Chakrabarty T, Miyazawa K, Nduom NA, Emmerling K. The kuru infectious agent is a unique geographic isolate distinct from Creutzfeldt–Jakob disease and scrapie agents. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 13529-13534. PMID: 19633190, PMCID: PMC2715327, DOI: 10.1073/pnas.0905825106.Peer-Reviewed Original ResearchConceptsSporadic Creutzfeldt-Jakob diseaseCreutzfeldt-Jakob diseaseBovine spongiform encephalopathyBSE agentHuman sporadic Creutzfeldt-Jakob diseaseInfectious agentsEpidemic bovine spongiform encephalopathyTSE agentsScrapie agentTransmissible spongiform encephalopathy agentsSpongiform encephalopathy agentKuru agentLymphoreticular involvementBrain neuropathologySporadic CJDGT1 cellsNormal miceInfected humansNeurodegenerative diseasesDiseaseViral receptorsInfectious neurodegenerative diseasesSheep scrapieSpongiform encephalopathiesHigh levels