2022
Concurrent targeting of glycolysis in bacteria and host cell inflammation in septic arthritis
Kwon H, Yu K, Cahill S, Alder K, Dussik C, Kim S, Sharma L, Back J, Oh I, Lee F. Concurrent targeting of glycolysis in bacteria and host cell inflammation in septic arthritis. EMBO Molecular Medicine 2022, 14: emmm202115284. PMID: 36354099, PMCID: PMC9728052, DOI: 10.15252/emmm.202115284.Peer-Reviewed Original ResearchConceptsDrug dimethyl fumarateSeptic arthritisIntracellular MRSABacterial joint infectionSoft tissue infectionsAnti-inflammatory effectsInfection-associated inflammationNovel therapeutic paradigmContext of infectionConventional antibiotic treatmentHost cellsAdjuvant administrationSurgical treatmentTissue infectionsClinical symptomsInflammatory machineryJoint infectionBacterial burdenAntibiotic treatmentCell inflammationHost inflammationArthritisInflammationIntraarticular inflammationTherapeutic paradigmTreating ‘Septic’ With Enhanced Antibiotics and ‘Arthritis’ by Mitigation of Excessive Inflammation
Kwon HK, Dussik CM, Kim SH, Kyriakides TR, Oh I, Lee FY. Treating ‘Septic’ With Enhanced Antibiotics and ‘Arthritis’ by Mitigation of Excessive Inflammation. Frontiers In Cellular And Infection Microbiology 2022, 12: 897291. PMID: 35755835, PMCID: PMC9218192, DOI: 10.3389/fcimb.2022.897291.Peer-Reviewed Original ResearchConceptsSeptic arthritisBacterial burdenAntibiotic treatmentMurine modelTherapeutic goalsConcurrent antimicrobial therapyDistinct therapeutic goalsGeneration of inflammationMRSA septic arthritisSeptic knee arthritisInflammatory joint conditionsArticular cartilageMitigation of inflammationPost-antibiotic treatmentNovel therapeutic strategiesSeptic arthritis modelArticular cartilage damageEx vivo modelArticular cartilage integrityInflammatory arthritisInhibitors of ERKInflammatory profileMRSA infectionSynovial tissueExcessive inflammation
2021
Next-Generation Sequencing for Pathogen Identification in Infected Foot Ulcers
Choi Y, Oda E, Waldman O, Sajda T, Beck C, Oh I. Next-Generation Sequencing for Pathogen Identification in Infected Foot Ulcers. Foot & Ankle Orthopaedics 2021, 6: 24730114211026933. PMID: 35097461, PMCID: PMC8702686, DOI: 10.1177/24730114211026933.Peer-Reviewed Original ResearchDiabetic foot ulcersFoot infectionsFoot ulcersNext-generation sequencingOrthopedic infectionsInfected diabetic foot ulcersFellowship-trained orthopedic footPostoperative antibiotic treatmentInfected foot ulcersStandard cultureConventional cultureWarrants further investigationManagement of footDFU patientsOrthopaedic FootOperative treatmentKnee amputationAntibiotic treatmentRay amputationOperative procedureAnkle surgeonsCommon pathogensAverage ageCommon microorganismsConcordance rate
2019
Evidence of differential microbiomes in healing versus non‐healing diabetic foot ulcers prior to and following foot salvage therapy
MacDonald A, Brodell JD, Daiss JL, Schwarz EM, Oh I. Evidence of differential microbiomes in healing versus non‐healing diabetic foot ulcers prior to and following foot salvage therapy. Journal Of Orthopaedic Research® 2019, 37: 1596-1603. PMID: 30908702, PMCID: PMC6659747, DOI: 10.1002/jor.24279.Peer-Reviewed Original ResearchConceptsSalvage therapyQuantitative polymerase chain reactionClinical outcomesClinical toolErythrocyte sedimentation rateFurther surgical interventionC-reactive proteinLower extremity amputationDiabetic foot ulcersEventual clinical outcomeImportant prognostic informationImportant clinical toolDFU patientsFoot salvageFoot ulcersDiabetic footExtremity amputationSurgical interventionPatient counselingAntibiotic treatmentPolymerase chain reactionPrognostic informationClinical signsMicrobiome analysisClinical significance
2017
Microbiome Analysis for Assessments of Treatment Response and Salvage Prognosis in Infected Diabetic Foot Ulcers
MacDonald A, Oh I, Grier A, Smith B, Daiss J, Gill S. Microbiome Analysis for Assessments of Treatment Response and Salvage Prognosis in Infected Diabetic Foot Ulcers. Foot & Ankle Orthopaedics 2017, 2: 2473011417s000060. DOI: 10.1177/2473011417s000060.Peer-Reviewed Original ResearchIntravenous antibiotic treatmentAntibiotic treatmentNon-traumatic lower extremity amputationsInfected diabetic foot ulcersAdequate prognostic informationPoor prognostic signDiabetic foot ulcersLower extremity amputationSignificant differencesMicrobiome analysisDFU infectionsFoot salvageWagner classificationMedical comorbiditiesCounseling patientsFoot ulcersInitial presentationPrognostic signPrognostic valueActive infectionDiabetic footPrognostic informationClinical signsWeek 8Surgical decisionClinical Application of Immunoassay for Identification of Staphylococcus aureus and Monitoring Treatment Response in Diabetic Foot Infection
Oh I, Smith B, Grier A, Gill S, Daiss J. Clinical Application of Immunoassay for Identification of Staphylococcus aureus and Monitoring Treatment Response in Diabetic Foot Infection. Foot & Ankle Orthopaedics 2017, 2: 2473011417s000065. DOI: 10.1177/2473011417s000065.Peer-Reviewed Original ResearchDiabetic foot infectionsFoot infectionsSA infectionsAntibiotic treatmentTreatment responseStaphylococcus aureusPathogenic activityAdequate prognostic informationNonspecific inflammatory markersIntravenous antibiotic treatmentWhole bloodMonitoring Treatment ResponseSigns of infectionStandard cultureAntibody-secreting cellsMicrobiome analysisFoot salvageSalvage therapySalvage treatmentSpecific diagnostic toolsInflammatory markersDiabetic patientsCounseling patientsClinical symptomsWeeks 0