2024
Latrophilin-2 mediates fluid shear stress mechanotransduction at endothelial junctions
Tanaka K, Chen M, Prendergast A, Zhuang Z, Nasiri A, Joshi D, Hintzen J, Chung M, Kumar A, Mani A, Koleske A, Crawford J, Nicoli S, Schwartz M. Latrophilin-2 mediates fluid shear stress mechanotransduction at endothelial junctions. The EMBO Journal 2024, 43: 3175-3191. PMID: 38886581, PMCID: PMC11294477, DOI: 10.1038/s44318-024-00142-0.Peer-Reviewed Original ResearchLatrophilin-2Affinity purification methodCell-cell junctionsHuman genetic dataPECAM-1SiRNA screenGenetic dataEndothelial cell response to fluid shear stressGA proteinsDownstream eventsEndothelial-specific knockoutG-proteinActivity assayShear stress mechanotransductionPlexin-D1Endothelial signalingJunctional complexesPurification methodVE-cadherinResponse to fluid shear stressVascular developmentGA residuesEndothelial junctionsGPCRsVEGF receptors
2023
Haematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium
Ghersi J, Baldissera G, Hintzen J, Luff S, Cheng S, Xia I, Sturgeon C, Nicoli S. Haematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium. Nature Cell Biology 2023, 25: 1135-1145. PMID: 37460694, PMCID: PMC10415179, DOI: 10.1038/s41556-023-01187-9.Peer-Reviewed Original ResearchConceptsHaemogenic endothelial cellsHaematopoietic stemPost-transcriptional repressionHuman pluripotent stem cellsProgenitor cell heterogeneityCell cycle statePluripotent stem cellsG2/MZebrafish leadsAorta-gonadBlood lineagesEmbryonic endotheliumExpansion of HSPCsEndothelial cellsProliferative outputDifferent cell cycle statesLymphoid progenitorsCell heterogeneityLineagesMiR-128Jag1bHSPCsMyeloid lineageStem cellsCycle state3067 – HEMATOPOIETIC STEM AND PROGENITOR CELL HETEROGENEITY IS INHERITED FROM THE EMBRYONIC ENDOTHELIUM.
Ghersi J, Baldissera G, Hintzen J, Luff S, Cheng S, Xia I, Sturgeon C, Nicoli S. 3067 – HEMATOPOIETIC STEM AND PROGENITOR CELL HETEROGENEITY IS INHERITED FROM THE EMBRYONIC ENDOTHELIUM. Experimental Hematology 2023, 124: s83. DOI: 10.1016/j.exphem.2023.06.174.Peer-Reviewed Original ResearchHematopoietic stem/progenitor cellsMiR-128Hemogenic endothelial cellsMature blood cellsHuman pluripotent stem cellsCell cycle stateStem/progenitor cellsPluripotent stem cellsG2/MHematopoietic transitionEmbryonic endotheliumProliferative outputDifferent cell cycle statesAdult stageLymphoid progenitorsCell heterogeneityLineagesJag1bMyeloid lineageStem cellsProgenitor cellsCycle stateBlood progenitorsHemECsWnt