2001
Reactivity of dog sera to whole-cell or recombinant antigens of Borrelia burgdorferi by ELISA and immunoblot analysis
Magnarelli LA, Levy SA, Ijdo JW, Wu C, Padula SJ, Fikrig E. Reactivity of dog sera to whole-cell or recombinant antigens of Borrelia burgdorferi by ELISA and immunoblot analysis. Journal Of Medical Microbiology 2001, 50: 889-895. PMID: 11599738, DOI: 10.1099/0022-1317-50-10-889.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayWhole cell antigenRecombinant antigensB. burgdorferiNon-vaccinated dogsPolyvalent enzyme-linked immunosorbent assaysCanine borreliosisTick-infested areasTotal immunoglobulinWhole-cell B. burgdorferiAntibody titresBorrelia burgdorferi sensu strictoVaccinated dogsJoint disordersImmune responseBurgdorferi sensu strictoAntigenImmunosorbent assaySerumDogsNatural infectionReactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Oliver JH, Fikrig E. Reactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis. American Journal Of Veterinary Research 2001, 62: 1365-9. PMID: 11560261, DOI: 10.2460/ajvr.2001.62.1365.Peer-Reviewed Original ResearchConceptsIgG antibodiesSerum samplesHGE agentGranulocytic ehrlichiosisClient-owned dogsDog serum samplesHuman granulocytic ehrlichiosis (HGE) agentEnzyme-linked immunosorbentPositive resultsWestern blot analysisTick-infested areasEhrlichia phagocytophila genogroupTotal immunoglobulinHorse serum samplesPromyelocytic leukemia cellsClinical relevanceHuman promyelocytic leukemia cellsProtein antigensIgMELISADogsLeukemia cellsAntibodiesBlot analysisAntigenEvaluation of a polyvalent enzyme-linked immunosorbent assay incorporating a recombinant p44 antigen for diagnosis of granulocytic ehrlichiosis in dogs and horses.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Fikrig E. Evaluation of a polyvalent enzyme-linked immunosorbent assay incorporating a recombinant p44 antigen for diagnosis of granulocytic ehrlichiosis in dogs and horses. American Journal Of Veterinary Research 2001, 62: 29-32. PMID: 11197555, DOI: 10.2460/ajvr.2001.62.29.Peer-Reviewed Original ResearchConceptsEquine samplesPolyvalent ELISAPolyvalent enzyme-linked immunosorbentGranulocytic ehrlichiosisIFA stainingEquine serum samplesE risticiiEhrlichia canisCanine serum samplesCanine samplesSpecific recombinant antigensE equiHorsesEhrlichiosisPercentage of samplesIndirect fluorescent antibody stainingRecombinant antigensDogsWhole cell antigenCanisEquiELISA resultsSerum samplesPresent studyFluorescent antibody staining
1997
Antibodies to Ehrlichia equi in dogs from the northeastern United States.
Magnarelli L, IJdo J, Anderson J, Madigan J, Dumler J, Fikrig E. Antibodies to Ehrlichia equi in dogs from the northeastern United States. Journal Of The American Veterinary Medical Association 1997, 211: 1134-7. PMID: 9364226, DOI: 10.2460/javma.1997.211.09.1134.Peer-Reviewed Original ResearchConceptsE equiIndirect fluorescent antibodyWestern blot analysisSerologic testing methodsFluorescent antibodyBlot analysisTick-infested areasSeropositive dogsIll dogsMarked leukopeniaNormal dogsSubclinical infectionSerologic analysisEtiologic agentLaboratory diagnosisEnd pointGranulocytic ehrlichiosisEhrlichia equiDogsAntibodiesHuman isolatesBorrelia burgdorferiDog serumMultiple pathogensEhrlichiosisSerologic diagnosis of canine and equine borreliosis: use of recombinant antigens in enzyme-linked immunosorbent assays
Magnarelli LA, Flavell RA, Padula SJ, Anderson JF, Fikrig E. Serologic diagnosis of canine and equine borreliosis: use of recombinant antigens in enzyme-linked immunosorbent assays. Journal Of Clinical Microbiology 1997, 35: 169-173. PMID: 8968901, PMCID: PMC229532, DOI: 10.1128/jcm.35.1.169-173.1997.Peer-Reviewed Original ResearchConceptsRecombinant antigensPolyvalent enzyme-linked immunosorbent assaysWhole-cell B. burgdorferiEnzyme-linked immunosorbent assayWestern blot analysisOuter surface protein ASensu strictoBlot analysisWhole cellsProtein AOSPFSerologic diagnosisBorrelia burgdorferi sensu strictoEquid seraBurgdorferi sensu strictoB. burgdorferiSurface protein AP41OspCWhole cell antigenEquine serumDog serumImmunosorbent assayOspBSerum samples
1995
Serologic responses of dogs naturally exposed to or vaccinated against Borrelia burgdorferi infection.
Barthold S, Levy S, Fikrig E, Bockenstedt L, Smith A. Serologic responses of dogs naturally exposed to or vaccinated against Borrelia burgdorferi infection. Journal Of The American Veterinary Medical Association 1995, 207: 1435-40. PMID: 7493871, DOI: 10.2460/javma.1995.207.11.1435.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, BacterialBacterial Outer Membrane ProteinsBacterial ProteinsBacterial VaccinesBiological AssayBlotting, WesternBorrelia burgdorferi GroupDog DiseasesDogsEnzyme-Linked Immunosorbent AssayFemaleImmune SeraImmunoglobulin GImmunoglobulin MLyme DiseaseMaleMiceMice, Inbred C3HVaccinationConceptsSerologic responseAntibiotic treatmentVaccinated dogsRecombinant outer surface proteinPassive protective activityBorrelia burgdorferi infectionMeans of ELISAImmunoblot analysisSeronegative dogsClinical responseSerologic testingClinical criteriaControl dogsAntibody responseMouse protectionB burgdorferiClinical trialsBurgdorferi infectionInfected dogsELISA titersProtective activityOuter surface proteinsUseful antigenDogsBorrelia burgdorferi
1993
Serologic analysis of dogs, horses, and cottontail rabbits for antibodies to an antigenic flagellar epitope of Borrelia burgdorferi
Fikrig E, Magnarelli LA, Chen M, Anderson JF, Flavell RA. Serologic analysis of dogs, horses, and cottontail rabbits for antibodies to an antigenic flagellar epitope of Borrelia burgdorferi. Journal Of Clinical Microbiology 1993, 31: 2451-2455. PMID: 7691874, PMCID: PMC265777, DOI: 10.1128/jcm.31.9.2451-2455.1993.Peer-Reviewed Original Research
1992
Spirochetes: vaccines, animal models and diagnostics
Simon M, Milward F, Lefebvre R, Schouls L, Fikrig E, Wasmoen T, Stover K, Menefee B, Robinson J. Spirochetes: vaccines, animal models and diagnostics. Research In Microbiology 1992, 143: 641-647. PMID: 1475524, DOI: 10.1016/0923-2508(92)90122-5.Peer-Reviewed Original Research