A) 40X magnification: a cluster of IL-6 positive cells within the portal tract (DAPI-blue, IL-6-red).
B) 40X magnification: very few IL-1β positive cells present within the portal tract (DAPI-blue, IL-1β-red).
C-D) 20X magnification: several IL-17A positive cells present within the portal tract (DAPI-blue, IL-17A-red) and co-expressed with FOXP3. Arrowheads point to FOXP3/IL17A co-expressing cells. (DAPI-blue, IL-17A-red, FOXP3 green). 60X magnification: Several CD4 positive cells seen that co-express with FOXP3 (DAPI-blue, CD4-green, FOXP3-red).
E-F) 20X magnification: the TH1 polarizing cytokine, IL-12 (DAPI-blue, IL-12-red) as well as its signature cytokine, IFN-γ (DAPI-blue, IFN-γ-red), present within portal inflammatory infiltrates.
G) 20X magnification: a few IFN-γ positive cells co-expressed with FOXP3 on same cell. Arrowheads point to FOXP3/IFN-γ co-expressing cells. (DAPI-blue, FOXP-green, IFN-γ-red).
(H) 60X magnification: IL-12 co-expressed with CD68 (DAPI-blue, IL-12-red, CD68-green); as well as IL-6 (40X magnification) (DAPI-blue, IL-12-red, IL-6-green).
(I) 20X magnification: CD14+ cells co-expressed with CD68+ cells (white arrows) (DAPI-blue, CD14-red, CD68-green).
(J) 20X magnification. Representative slides from isotype controls from one patient are shown.