Research Departments & Organizations
Mobile @ Yale
My research focuses on translational studies of primary human cells in disease susceptibility and divergent clinical outcomes. We employ emerging technology in investigations and have published several methods using novel technology to advance translational studies. I launched the Yale CyTOF Core facility and work with investigators to facilitate use of this novel platform and a prototype imaging CyTOF (IMC) which detects >40 markers in tissue. In collaborative studies, we evaluate immune responses broadly in asthma, dengue virus, hepatitis C virus, humanized mice, leptospirosis, and Zika virus infections.
The focus of our lab’s research is on innate immunity, specifically the interaction of macrophages, neutrophils, and dendritic cells with pathogens. We study the effect of aging and immunosuppression on immune responses and specifically on the expression and efficiency of Toll-Like receptors. In our studies from a large cohort of human subjects, we have shown that older donors express lower levels of certain TLRs and show dysregulation of immune pathways in aging.
I am the Director of the CyTOF facility, which houses Two Helios instruments, one is dedicated to the Imaging mass Cytometer (IMC). CyTOF is a mass-spec based cell analyzer which provides multi-dimensional single cell data from cells in suspensions and recently also in solid tissue (IMC). CyTOF improves on fluorescent flow cytometry by allowing detection of a greater number of markers per sample (~30-40 instead of 4-8) without background signals, and most importantly, without overlap between signals.
Specialized Terms: Innate Immunity; Macrophage; Neutrophil; Aging; West Nile Virus
|Addictive Behaviors, HIV/AIDS, Infectious Diseases||Impact of HIV Infection on Immunologic, Transcriptomic, and Metabolomic Signatures|
Age-related changes in expression and signaling of TAM receptor inflammatory regulators in monocytes
Wang X, Malawista A, Qian F, Ramsey C, Allore HG, Montgomery RR. Oncotarget 2018; 9:9572-80. 2018
Identification of genetic variants associated with dengue or West Nile virus disease: a systematic review and meta-analysis.
Cahill ME, Conley S, DeWan AT, Montgomery RR. Identification of genetic variants associated with dengue or West Nile virus disease: a systematic review and meta-analysis. BMC Infectious Diseases 2018, 18:282. 2018
Multiparameter single cell profiling of airway inflammatory cells.
Yao, Y., T. Welp, Q. Liu, N. Niu, X. Wang, C. J. Britto, S. Krishnaswamy, G. L. Chupp, and R. R. Montgomery. 2017. Cytometry. Part B, Clinical cytometry 92: 12-20. 2017
West Nile Virus Seroprevalence, Connecticut, USA, 2000-2014.
Cahill ME, Yao Y, Nock D, Armstrong PM, Andreadis TG, Diuk-Wasser MA, Montgomery RR. West Nile Virus Seroprevalence, Connecticut, USA, 2000-2014. Emerging Infectious Diseases 2017, 23:708-710. 2017
High standards for high dimensional investigations.
Montgomery RR. High standards for high dimensional investigations. Cytometry. Part A : The Journal Of The International Society For Analytical Cytology 2016, 89:886-888. 2016
Human NK cell repertoire diversity reflects immune experience and correlates with viral susceptibility.
Strauss-Albee DM, Fukuyama J, Liang EC, Yao Y, Jarrell JA, Drake AL, Kinuthia J, Montgomery RR, John-Stewart G, Holmes S, Blish CA. Human NK cell repertoire diversity reflects immune experience and correlates with viral susceptibility. Science Translational Medicine 2015, 7:297ra115. 2015
Systems immunology reveals markers of susceptibility to West Nile virus infection.
Qian F, Goel G, Meng H, Wang X, You F, Devine L, Raddassi K, Garcia MN, Murray KO, Bolen CR, Gaujoux R, Shen-Orr SS, Hafler D, Fikrig E, Xavier R, Kleinstein SH, Montgomery RR. Systems immunology reveals markers of susceptibility to West Nile virus infection. Clinical And Vaccine Immunology : CVI 2015, 22:6-16. 2015
CyTOF supports efficient detection of immune cell subsets from small samples.
Yao Y, Liu R, Shin MS, Trentalange M, Allore H, Nassar A, Kang I, Pober JS, Montgomery RR. CyTOF supports efficient detection of immune cell subsets from small samples. Journal Of Immunological Methods 2014, 415:1-5. 2014
Reduced bioenergetics and toll-like receptor 1 function in human polymorphonuclear leukocytes in aging.
Qian F, Guo X, Wang X, Yuan X, Chen S, Malawista SE, Bockenstedt LK, Allore HG, Montgomery RR. Reduced bioenergetics and toll-like receptor 1 function in human polymorphonuclear leukocytes in aging. Aging 2014, 6:131-9. 2014
Impaired toll-like receptor 3-mediated immune responses from macrophages of patients chronically infected with hepatitis C virus.
Qian F, Bolen CR, Jing C, Wang X, Zheng W, Zhao H, Fikrig E, Bruce RD, Kleinstein SH, Montgomery RR. Impaired toll-like receptor 3-mediated immune responses from macrophages of patients chronically infected with hepatitis C virus. Clinical And Vaccine Immunology : CVI 2013, 20:146-55. 2013
Age-associated elevation in TLR5 leads to increased inflammatory responses in the elderly.
Qian F, Wang X, Zhang L, Chen S, Piecychna M, Allore H, Bockenstedt L, Malawista S, Bucala R, Shaw AC, Fikrig E, Montgomery RR. Age-associated elevation in TLR5 leads to increased inflammatory responses in the elderly. Aging Cell 2012, 11:104-10. 2012