Research Departments & Organizations
With more than 60% marketed drugs targeting membrane proteins, a detailed understanding of structural and functional regulation of membrane protein is of pivotal interest. Recently, we have made remarkable progress towards obtaining structures of independent membrane proteins. Nevertheless, in the crowded milieu of the membrane, membrane proteins form a large number of dynamic and transient complexes with other soluble and membrane proteins, lipids and various other endogenous and extraneous regulators, which in turn govern cellular physiology. Hence, discovering these complexes and deconvoluting their spatiotemporal evolution along the progression of cellular cycle pose the next big challenge. Using native mass spectrometry in combination with other experimental and theoretical approaches, the lab provides a novel top down deconstructive vantage point to this problem. Our approach enables us to detect membrane protein complexes directly from the native environment, identify the novel interacting partners, and subsequently track their spatiotemporal progression. Applying these to dynamic macro-molecular membrane associated system our aim is to delineate the molecular mechanisms behind various cellular signalling pathways.
We work closely with researchers with varied expertise. While we love the physicists and the chemists who develop novel experimental tools, we cannot live without the biologists and the medics who use these cutting-edge techniques to solve the problems most pertinent to humankind. We even have an anthropologist. We are always interested in innovators, the creative thinkers, the dreamers, and the doers.
If you want to discover the world of native mass spectrometry, we want to hear from you.
If you have ideas, we want to hear from you.
If you are merely curious, we want to hear from you.
We currently have multiple openings for postdocs, graduate students and research assistants. Contact me directly with your CV and research interests. Click the lab website link for more details
Selected relevant publications:
- Gupta K, Li J, Liko I, Gault J, Bechara C, Wu D, Hopper JTS, Giles K, Benesch JLP, Robinson CV. Identifying key membrane protein lipid interactions using mass spectrometry. Nature Protocol, 2018:13:1106
- Ambrose S, Housden NG, Gupta K, Fan J, White P, Yen H, Marcoux J, Kleanthous C, Hopper JTS, Robinson CV. Native desorption electrospray ionization liberates soluble and membrane protein complexes from surfaces Angew. Chem. Int. Ed. (published online, doi: 10.1002/anie.201704849)
- Gupta K, Donlan JAC, Hopper JTS, Uzdavinys P, Landreh M, Struwe WB, Drew D, Baldwin AJ, Phillip J. Stansfeld PJ, Robinson CV. The role of interfacial lipids in stabilizing membrane protein oligomers. Nature 2017:541: 421
- Landreh M, Marklund EG, Uzdavinys P, Degiocomi MT, Gault J, Coincon M, Liko I, Gupta K, Benesch JLP, Drew David, Robinson CV. Integrating mass spectrometry with MD simulations reveals the role of lipids in Na+/H+ antiporters. Nature Comm. 2017:8: 13993
- Gault J, Donlan JAC, Liko I, Hopper JTS, Gupta K, Housden NG, Struwe WB, Marty MT, Mize T, Bechara C, Zhu Y, Wu B, Kleanthous C, Belov M, Damoc E, Makarov A, Robinson CV. High-resolution mass spectrometry of small molecules bound to membrane proteins. Nature Methods 2016:13: 336