John Greg Howe, PhD

Professor; Director, Molecular Diagnostics Laboratory

Research Departments & Organizations

Laboratory Medicine: Clinical Flow Cytometry Laboratory | General Hematology | Molecular Diagnostics Laboratory

Office of Cooperative Research

Research Interests

Epstein-Barr Virus Infections; Factor V; Flow Cytometry; Medical Laboratory Science; Molecular Diagnostic Techniques; Prothrombin

Research Summary

The mission of the Molecular Diagnostics Laboratory is to develop and bring into clinical use already published molecular pathology assays as well as to develop new and innovative molecular tests for the diagnosis of disease and the monitoring of therapy. Various tests have been developed and are in clinical use such as detection of mutations in the prothrombin and Factor V genes that are associated with coagulation abnormalities and tests to determine monoclonality in patients with suspected hematological malignancies. Previously, we have developed an in situ hybridization assay to test for the presence of Epstein-Barr virus (EBV) in fixed tissue and have used this test to study the role of EBV in several diseases. Using the in situ hybridization method in conjunction with flow cytometry we have been able to demonstrate the utility of a method to quantitate the number of EBV containing cells. We have applied quantitative polymerase chain reaction (QPCR) methods to the measurement of EBV viral load in allogenic transplant patients and have further refined the method with the development of a real-time quantitative EBV PCR assay.

Specialized Terms: Molecular Diagnostics; Hematopathology

Selected Publications

See list of PubMed publications

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Contact Info

John Greg Howe, PhD
Mailing Address
Laboratory MedicinePO Box 208035
333 Cedar Street

New Haven, CT 06520-8035

Molecular Diagnostics