Research Departments & Organizations
The main interest in our laboratory is to elucidate the structure-function and regulation of ion channels from the ENaC/Degenerin family specifically by a set of S/T kinases known as glococorticoid-induced kinases (Sgk). Some of these channels such as ENaC are expressed in kidney where they retain sodium and thereby are key contributors for the maintenance and regulation of blood pressure.
The mammalian Degenerins or ASICs are channels expressed in the peripheral and central nervous system where they may regulate membrane excitability and thus contribute to many neuronal processes. We employ a broad variety of approaches that include molecular and cell biological techniques and electrophysiological measurements of channel activity by patch clamp techniques on cultured cells and tissues. We also develop mouse models expressing mutant channels or Sgk kinases to examine the physiological and pathophysiological consequences of altered channel function in blood pressure and in the nervous system.
Specialized Terms: Renal Function; Epithelial Sodium Channels
Extensive Research Description
our laboratory we examine the structure, function and regulation of two
types of sodium channels: the Epithelial Na + Channel (ENaC) and Acid
Sensing Ion Channels (ASIC). These proteins are structurally related
but serve very different functions. ENaC is expressed in the apical
side of epithelial cells involved in sodium absorption such as in
kidney, lung and colon. Mutations in the human ENaC genes produce
disorders of whole-body sodium balance and are characterized by
hypertension or sodium wasting. Our current work centers on mechanisms
that regulate activity, expression and traffic of ENaC in epithelial
cells specifically, on the role of a kinase known as serum-and
glucocorticoid-induced kinase or Sgk1.
ASICs are expressed in neurons of the central and peripheral nervous
systems. External protons gate ASIC but other stimuli are likely to be
more important physiological agonists. These channels have been
implicated in many functions including nociception, mechanoperception
and modulation of synaptic transmission. Our work centers in the
elucidation of the biophysical properties and gating of ASICs from many
use a broad range of experimental approaches and techniques that
include electrophysiology (patch-clamp, two-electrode voltage clamp,
short-circuit current), cloning of channels from evolutionary distant
species, modifications of channels and their expression in oocytes,
cell lines and in mice either trangenics or knockins.
Multiple translational isoforms give functional specificity to serum- and glucocorticoid-induced kinase 1.
Arteaga MF, Alvarez de la Rosa D, Alvarez JA, Canessa CM. Multiple translational isoforms give functional specificity to serum- and glucocorticoid-induced kinase 1. Molecular Biology Of The Cell 2007, 18:2072-80. 2007
An amphipathic helix targets serum and glucocorticoid-induced kinase 1 to the endoplasmic reticulum-associated ubiquitin-conjugation machinery.
Arteaga MF, Wang L, Ravid T, Hochstrasser M, Canessa CM. An amphipathic helix targets serum and glucocorticoid-induced kinase 1 to the endoplasmic reticulum-associated ubiquitin-conjugation machinery. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103:11178-83. 2006
Gating of acid-sensitive ion channel-1: release of Ca2+ block vs. allosteric mechanism.
Zhang P, Sigworth FJ, Canessa CM. Gating of acid-sensitive ion channel-1: release of Ca2+ block vs. allosteric mechanism. The Journal Of General Physiology 2006, 127:109-17. 2006
SGK1 activates Na+-K+-ATPase in amphibian renal epithelial cells.
Alvarez de la Rosa D, Gimenez I, Forbush B, Canessa CM. SGK1 activates Na+-K+-ATPase in amphibian renal epithelial cells. American Journal Of Physiology. Cell Physiology 2006, 290:C492-8. 2006
Proton sensitivity of ASIC1 appeared with the rise of fishes by changes of residues in the region that follows TM1 in the ectodomain of the channel.
Coric T, Zheng D, Gerstein M, Canessa CM. Proton sensitivity of ASIC1 appeared with the rise of fishes by changes of residues in the region that follows TM1 in the ectodomain of the channel. The Journal Of Physiology 2005, 568:725-35. 2005