Research Departments & Organizations
My research is focused on infections transmitted by ticks, in particular Lyme Disease, Babesia, and relapsing fever. I am interested in understanding the diseases caused by these infections, in particular those mediated by the immune system. I am also interested in developing better diagnostics for these infections.
Specialized Terms: Vector-borne diseases; Lyme disease; Babesia; Relapsing fever spirochetes; Immunology; Diagnostics; Ticks; Intra-vital imaging; Optical tweezers
Extensive Research Description
My current research is primarily focused on understanding the immune responses and disease pathogenesis during B. burgdorferi infections. I am particularly interested in early immune responses during infection, and the role of marginal zone B cells. We are using several novel technologies to study the spirochetes including intra-vital microscopy and optical tweezers. With intra-vital microscopy we are studying both the motility and location of spirochetes in infected mice. We are also using intra-vital microscopy to analyze the joints of the mice in the hopes of better understanding the development of Lyme arthritis. In collaboration with the Dufresne lab in the Engineering and Physics departments, we are using optical tweezers to better understand the biomechanics of spirochete movement. Spirochete movement is crucial for the bacteria's ability to invade different tissues, and also plays a role in their ability to escape immune destruction.
In addition to studying immune responses to infection, we are also working to develop better diagnostic tests for Lyme disease based on our deeper understanding of the types of immune responses that are generated. We are collaborating with L2 diagnostics on several different projects.
In addition to Lyme disease, Ixodes ticks carry several other pathogens and we are also interested in studying the Babesia parasite, as well as the newly identified human pathogen Borrelia miyamotoi.
Natural antibody affects survival of the spirochete Borrelia burgdorferi within feeding ticks.
Belperron AA, Bockenstedt LK. Natural antibody affects survival of the spirochete Borrelia burgdorferi within feeding ticks. Infection And Immunity 2001, 69:6456-62. 2001
Infection-induced marginal zone B cell production of Borrelia hermsii-specific antibody is impaired in the absence of CD1d.
Belperron AA, Dailey CM, Bockenstedt LK. Infection-induced marginal zone B cell production of Borrelia hermsii-specific antibody is impaired in the absence of CD1d. Journal Of Immunology (Baltimore, Md. : 1950) 2005, 174:5681-6. 2005
Marginal zone B-cell depletion impairs murine host defense against Borrelia burgdorferi infection.
Belperron AA, Dailey CM, Booth CJ, Bockenstedt LK. Marginal zone B-cell depletion impairs murine host defense against Borrelia burgdorferi infection. Infection And Immunity 2007, 75:3354-60. 2007
The caspase 1 inflammasome is not required for control of murine Lyme borreliosis.
Liu N, Belperron AA, Booth CJ, Bockenstedt LK. The caspase 1 inflammasome is not required for control of murine Lyme borreliosis. Infection And Immunity 2009, 77:3320-7. 2009
The heterogeneous motility of the Lyme disease spirochete in gelatin mimics dissemination through tissue.
Harman MW, Dunham-Ems SM, Caimano MJ, Belperron AA, Bockenstedt LK, Fu HC, Radolf JD, Wolgemuth CW. The heterogeneous motility of the Lyme disease spirochete in gelatin mimics dissemination through tissue. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109:3059-64. 2012
Spirochete antigens persist near cartilage after murine Lyme borreliosis therapy.
Bockenstedt LK, Gonzalez DG, Haberman AM, Belperron AA. Spirochete antigens persist near cartilage after murine Lyme borreliosis therapy. The Journal Of Clinical Investigation 2012, 122:2652-60. 2012