Wenxiang Cao, PhD
Research Scientist
Research & Publications
Biography
News
Coauthors
Selected Publications
- Cooperative ligand binding to a double-stranded Ising lattice—Application to cofilin binding to actin filamentsCao W, Taylor E, De La Cruz E. Cooperative ligand binding to a double-stranded Ising lattice—Application to cofilin binding to actin filaments. PNAS Nexus 2023, 2: pgad331. PMID: 37885622, PMCID: PMC10599439, DOI: 10.1093/pnasnexus/pgad331.
- Friction patterns guide actin network contractionColin A, Orhant-Prioux M, Guérin C, Savinov M, Cao W, Vianay B, Scarfone I, Roux A, De La Cruz E, Mogilner A, Théry M, Blanchoin L. Friction patterns guide actin network contraction. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2300416120. PMID: 37725653, PMCID: PMC10523593, DOI: 10.1073/pnas.2300416120.
- Twist response of actin filamentsBibeau J, Pandit N, Gray S, Nejad N, Sindelar C, Cao W, De La Cruz E. Twist response of actin filaments. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2208536120. PMID: 36656858, PMCID: PMC9942836, DOI: 10.1073/pnas.2208536120.
- Flagella-like beating of actin bundles driven by self-organized myosin wavesPochitaloff M, Miranda M, Richard M, Chaiyasitdhi A, Takagi Y, Cao W, De La Cruz E, Sellers J, Joanny J, Jülicher F, Blanchoin L, Martin P. Flagella-like beating of actin bundles driven by self-organized myosin waves. Nature Physics 2022, 18: 1240-1247. PMID: 37396880, PMCID: PMC10312380, DOI: 10.1038/s41567-022-01688-8.
- The nucleoporin Gle1 activates DEAD-box protein 5 (Dbp5) by promoting ATP binding and accelerating rate limiting phosphate releaseGray S, Cao W, Montpetit B, De La Cruz EM. The nucleoporin Gle1 activates DEAD-box protein 5 (Dbp5) by promoting ATP binding and accelerating rate limiting phosphate release. Nucleic Acids Research 2022, 50: 3998-4011. PMID: 35286399, PMCID: PMC9023272, DOI: 10.1093/nar/gkac164.
- Structural basis of fast- and slow-severing actin–cofilactin boundariesHocky GM, Sindelar CV, Cao W, Voth GA, De La Cruz EM. Structural basis of fast- and slow-severing actin–cofilactin boundaries. Journal Of Biological Chemistry 2021, 296: 100337. PMID: 33508320, PMCID: PMC7961102, DOI: 10.1016/j.jbc.2021.100337.
- Improving the Pharmacodynamics and In Vivo Activity of ENPP1‐Fc Through Protein and Glycosylation EngineeringStabach PR, Zimmerman K, Adame A, Kavanagh D, Saeui CT, Agatemor C, Gray S, Cao W, De La Cruz EM, Yarema KJ, Braddock DT. Improving the Pharmacodynamics and In Vivo Activity of ENPP1‐Fc Through Protein and Glycosylation Engineering. Clinical And Translational Science 2020, 14: 362-372. PMID: 33064927, PMCID: PMC7877847, DOI: 10.1111/cts.12887.
- Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branchesPandit NG, Cao W, Bibeau J, Johnson-Chavarria EM, Taylor EW, Pollard TD, De La Cruz EM. Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 13519-13528. PMID: 32461373, PMCID: PMC7306818, DOI: 10.1073/pnas.1911183117.
- Directional allosteric regulation of protein filament lengthJermyn AS, Cao W, Elam WA, De La Cruz EM, Lin MM. Directional allosteric regulation of protein filament length. Physical Review E 2020, 101: 032409. PMID: 32290018, PMCID: PMC7758089, DOI: 10.1103/physreve.101.032409.
- Structures of cofilin-induced structural changes reveal local and asymmetric perturbations of actin filamentsHuehn AR, Bibeau JP, Schramm AC, Cao W, De La Cruz EM, Sindelar CV. Structures of cofilin-induced structural changes reveal local and asymmetric perturbations of actin filaments. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 1478-1484. PMID: 31900364, PMCID: PMC6983403, DOI: 10.1073/pnas.1915987117.
- Severed Actin and Microtubules with Motors Walking All Over Them: Cryo-EM Studies of Seriously Perturbed Helical AssembliesDebs G, Huehn A, Cha M, Liu X, Elam W, Cao W, De La Cruz E, Sindelar C. Severed Actin and Microtubules with Motors Walking All Over Them: Cryo-EM Studies of Seriously Perturbed Helical Assemblies. Microscopy And Microanalysis 2019, 25: 1362-1363. DOI: 10.1017/s1431927619007542.
- Active cargo positioning in antiparallel transport networksRichard M, Blanch-Mercader C, Ennomani H, Cao W, De La Cruz EM, Joanny JF, Jülicher F, Blanchoin L, Martin P. Active cargo positioning in antiparallel transport networks. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 14835-14842. PMID: 31289230, PMCID: PMC6660773, DOI: 10.1073/pnas.1900416116.
- Nup159 Weakens Gle1 Binding to Dbp5 But Does Not Accelerate ADP ReleaseWong EV, Gray S, Cao W, Montpetit R, Montpetit B, De La Cruz EM. Nup159 Weakens Gle1 Binding to Dbp5 But Does Not Accelerate ADP Release. Journal Of Molecular Biology 2018, 430: 2080-2095. PMID: 29782832, PMCID: PMC6003625, DOI: 10.1016/j.jmb.2018.05.025.
- The actin filament twist changes abruptly at boundaries between bare and cofilin-decorated segmentsHuehn A, Cao W, Elam WA, Liu X, De La Cruz EM, Sindelar CV. The actin filament twist changes abruptly at boundaries between bare and cofilin-decorated segments. Journal Of Biological Chemistry 2018, 293: 5377-5383. PMID: 29463680, PMCID: PMC5900768, DOI: 10.1074/jbc.ac118.001843.
- Cofilin Induces a Local Change in the Twist of Actin FilamentsHuehn A, Cao W, Elam W, De La Cruz E, Sindelar C. Cofilin Induces a Local Change in the Twist of Actin Filaments. Biophysical Journal 2018, 114: 145a. DOI: 10.1016/j.bpj.2017.11.812.
- Phosphomimetic S3D cofilin binds but only weakly severs actin filamentsElam WA, Cao W, Kang H, Huehn A, Hocky GM, Prochniewicz E, Schramm AC, Negrón K, Garcia J, Bonello TT, Gunning PW, Thomas DD, Voth GA, Sindelar CV, De La Cruz EM. Phosphomimetic S3D cofilin binds but only weakly severs actin filaments. Journal Of Biological Chemistry 2017, 292: 19565-19579. PMID: 28939776, PMCID: PMC5712599, DOI: 10.1074/jbc.m117.808378.
- Neuronal Calcium Sensor 1 Has Two Variants with Distinct Calcium Binding CharacteristicsWang B, Boeckel GR, Huynh L, Nguyen L, Cao W, De La Cruz EM, Kaftan EJ, Ehrlich BE. Neuronal Calcium Sensor 1 Has Two Variants with Distinct Calcium Binding Characteristics. PLOS ONE 2016, 11: e0161414. PMID: 27575489, PMCID: PMC5004852, DOI: 10.1371/journal.pone.0161414.
- Architecture and Connectivity Govern Actin Network ContractilityEnnomani H, Letort G, Guérin C, Martiel JL, Cao W, Nédélec F, De La Cruz EM, Théry M, Blanchoin L. Architecture and Connectivity Govern Actin Network Contractility. Current Biology 2016, 26: 616-626. PMID: 26898468, PMCID: PMC4959279, DOI: 10.1016/j.cub.2015.12.069.
- Pi Release Limits the Intrinsic and RNA-Stimulated ATPase Cycles of DEAD-Box Protein 5 (Dbp5)Wong EV, Cao W, Vörös J, Merchant M, Modis Y, Hackney DD, Montpetit B, De La Cruz EM. Pi Release Limits the Intrinsic and RNA-Stimulated ATPase Cycles of DEAD-Box Protein 5 (Dbp5). Journal Of Molecular Biology 2015, 428: 492-508. PMID: 26730886, PMCID: PMC4744555, DOI: 10.1016/j.jmb.2015.12.018.
- ENPP1-Fc prevents mortality and vascular calcifications in rodent model of generalized arterial calcification of infancyAlbright RA, Stabach P, Cao W, Kavanagh D, Mullen I, Braddock AA, Covo MS, Tehan M, Yang G, Cheng Z, Bouchard K, Yu ZX, Thorn S, Wang X, Folta-Stogniew EJ, Negrete A, Sinusas AJ, Shiloach J, Zubal G, Madri JA, De La Cruz EM, Braddock DT. ENPP1-Fc prevents mortality and vascular calcifications in rodent model of generalized arterial calcification of infancy. Nature Communications 2015, 6: 10006. PMID: 26624227, PMCID: PMC4686714, DOI: 10.1038/ncomms10006.
- Site-Specific Cation Release Drives Actin Filament Severing by Vertebrate CofilinKang H, Bradley M, Cao W, Zhou K, Grintsevich E, Michelot A, Reisler E, Sindelar C, Hochstrasser M, De La Cruz E. Site-Specific Cation Release Drives Actin Filament Severing by Vertebrate Cofilin. Biophysical Journal 2015, 108: 24a-25a. DOI: 10.1016/j.bpj.2014.11.159.
- Kinetics and Thermodynamics of the ATPase Cycle of the DEAD-Box Protein Dbp5Wong E, Cao W, Montpetit B, De La Cruz E. Kinetics and Thermodynamics of the ATPase Cycle of the DEAD-Box Protein Dbp5. Biophysical Journal 2015, 108: 225a. DOI: 10.1016/j.bpj.2014.11.1243.
- Site-specific cation release drives actin filament severing by vertebrate cofilinKang H, Bradley MJ, Cao W, Zhou K, Grintsevich EE, Michelot A, Sindelar CV, Hochstrasser M, De La Cruz EM. Site-specific cation release drives actin filament severing by vertebrate cofilin. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 17821-17826. PMID: 25468977, PMCID: PMC4273407, DOI: 10.1073/pnas.1413397111.
- Multi-Platform Compatible Software for Analysis of Polymer Bending MechanicsGraham JS, McCullough BR, Kang H, Elam WA, Cao W, De La Cruz EM. Multi-Platform Compatible Software for Analysis of Polymer Bending Mechanics. PLOS ONE 2014, 9: e94766. PMID: 24740323, PMCID: PMC3989245, DOI: 10.1371/journal.pone.0094766.
- Molecular Basis of Purinergic Signal Metabolism by Ectonucleotide Pyrophosphatase/Phosphodiesterases 4 and 1 and Implications in Stroke*♦Albright RA, Ornstein DL, Cao W, Chang WC, Robert D, Tehan M, Hoyer D, Liu L, Stabach P, Yang G, De La Cruz EM, Braddock DT. Molecular Basis of Purinergic Signal Metabolism by Ectonucleotide Pyrophosphatase/Phosphodiesterases 4 and 1 and Implications in Stroke*♦. Journal Of Biological Chemistry 2013, 289: 3294-3306. PMID: 24338010, PMCID: PMC3916532, DOI: 10.1074/jbc.m113.505867.
- Quantitative full time course analysis of nonlinear enzyme cycling kineticsCao W, De La Cruz EM. Quantitative full time course analysis of nonlinear enzyme cycling kinetics. Scientific Reports 2013, 3: 2658. PMID: 24029878, PMCID: PMC3772379, DOI: 10.1038/srep02658.
- NPP4 is a procoagulant enzyme on the surface of vascular endotheliumAlbright RA, Chang WC, Robert D, Ornstein DL, Cao W, Liu L, Redick ME, Young JI, De La Cruz EM, Braddock DT. NPP4 is a procoagulant enzyme on the surface of vascular endothelium. Blood 2012, 120: 4432-4440. PMID: 22995898, PMCID: PMC4017314, DOI: 10.1182/blood-2012-04-425215.
- Actin Network Architecture Can Determine Myosin Motor ActivityReymann AC, Boujemaa-Paterski R, Martiel JL, Guérin C, Cao W, Chin HF, De La Cruz EM, Théry M, Blanchoin L. Actin Network Architecture Can Determine Myosin Motor Activity. Science 2012, 336: 1310-1314. PMID: 22679097, PMCID: PMC3649007, DOI: 10.1126/science.1221708.
- Plus‐end directed myosins accelerate actin filament sliding by single‐headed myosin VIRamamurthy B, Cao W, De La Cruz EM, Mooseker MS. Plus‐end directed myosins accelerate actin filament sliding by single‐headed myosin VI. Cytoskeleton 2012, 69: 59-69. PMID: 22213699, PMCID: PMC3327287, DOI: 10.1002/cm.21002.
- Molecular Structure and Biological Activity of NPP-4, An Endothelial Cell Surface Pyrophosphatase/ Phosphodiesterase That Stimulates Platelet Aggregation and Secretion Via Liberation of ADP Upon Hydrolysis of Diadenosine TriphosphateOrnstein D, Albright R, Chang W, Robert D, Cao W, De La Cruz E, Braddock D. Molecular Structure and Biological Activity of NPP-4, An Endothelial Cell Surface Pyrophosphatase/ Phosphodiesterase That Stimulates Platelet Aggregation and Secretion Via Liberation of ADP Upon Hydrolysis of Diadenosine Triphosphate. Blood 2011, 118: 701. DOI: 10.1182/blood.v118.21.701.701.
- Actin Filament Dynamics in the Actomyosin VI Complex Is Regulated Allosterically by Calcium–Calmodulin Light ChainProchniewicz E, Pierre A, McCullough BR, Chin HF, Cao W, Saunders LP, Thomas DD, De La Cruz EM. Actin Filament Dynamics in the Actomyosin VI Complex Is Regulated Allosterically by Calcium–Calmodulin Light Chain. Journal Of Molecular Biology 2011, 413: 584-592. PMID: 21910998, PMCID: PMC3633491, DOI: 10.1016/j.jmb.2011.08.058.
- Cofilin-Linked Changes in Actin Filament Flexibility Promote SeveringMcCullough BR, Grintsevich EE, Chen CK, Kang H, Hutchison AL, Henn A, Cao W, Suarez C, Martiel JL, Blanchoin L, Reisler E, De La Cruz EM. Cofilin-Linked Changes in Actin Filament Flexibility Promote Severing. Biophysical Journal 2011, 101: 151-159. PMID: 21723825, PMCID: PMC3127193, DOI: 10.1016/j.bpj.2011.05.049.
- Kinetic Analysis of Autotaxin Reveals Substrate-specific Catalytic Pathways and a Mechanism for Lysophosphatidic Acid Distribution*Saunders LP, Cao W, Chang WC, Albright RA, Braddock DT, De La Cruz EM. Kinetic Analysis of Autotaxin Reveals Substrate-specific Catalytic Pathways and a Mechanism for Lysophosphatidic Acid Distribution*. Journal Of Biological Chemistry 2011, 286: 30130-30141. PMID: 21719699, PMCID: PMC3191052, DOI: 10.1074/jbc.m111.246884.
- Mechanism of Mss116 ATPase Reveals Functional Diversity of DEAD-Box ProteinsCao W, Coman MM, Ding S, Henn A, Middleton ER, Bradley MJ, Rhoades E, Hackney DD, Pyle AM, De La Cruz EM. Mechanism of Mss116 ATPase Reveals Functional Diversity of DEAD-Box Proteins. Journal Of Molecular Biology 2011, 409: 399-414. PMID: 21501623, PMCID: PMC3125984, DOI: 10.1016/j.jmb.2011.04.004.
- Pathway of ATP utilization and duplex rRNA unwinding by the DEAD-box helicase, DbpAHenn A, Cao W, Licciardello N, Heitkamp SE, Hackney DD, De La Cruz EM. Pathway of ATP utilization and duplex rRNA unwinding by the DEAD-box helicase, DbpA. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 4046-4050. PMID: 20160110, PMCID: PMC2840157, DOI: 10.1073/pnas.0913081107.
- Kinetic Mechanism of Duplex rRNA Unwinding by the DEAD-box Protein, DbpAHenn A, Cao W, De La Cruz E. Kinetic Mechanism of Duplex rRNA Unwinding by the DEAD-box Protein, DbpA. Biophysical Journal 2009, 96: 414a. DOI: 10.1016/j.bpj.2008.12.2116.
- Widely Distributed Residues in Thymosin β4 Are Critical for Actin BindingAu JK, Olivares AO, Henn A, Cao W, Safer D, De La Cruz EM. Widely Distributed Residues in Thymosin β4 Are Critical for Actin Binding. Biochemistry 2008, 47: 4181-4188. PMID: 18327913, PMCID: PMC2587058, DOI: 10.1021/bi701769u.
- The ATPase Cycle Mechanism of the DEAD-box rRNA Helicase, DbpAHenn A, Cao W, Hackney DD, De La Cruz EM. The ATPase Cycle Mechanism of the DEAD-box rRNA Helicase, DbpA. Journal Of Molecular Biology 2007, 377: 193-205. PMID: 18237742, PMCID: PMC2359651, DOI: 10.1016/j.jmb.2007.12.046.
- Fluorescence of 2-aminopurine reveals rapid conformational changes in the RB69 DNA polymerase-primer/template complexes upon binding and incorporation of matched deoxynucleoside triphosphatesZhang H, Cao W, Zakharova E, Konigsberg W, De La Cruz EM. Fluorescence of 2-aminopurine reveals rapid conformational changes in the RB69 DNA polymerase-primer/template complexes upon binding and incorporation of matched deoxynucleoside triphosphates. Nucleic Acids Research 2007, 35: 6052-6062. PMID: 17766250, PMCID: PMC2094073, DOI: 10.1093/nar/gkm587.
- Energetics and Kinetics of Cooperative Cofilin–Actin Filament InteractionsCao W, Goodarzi JP, De La Cruz EM. Energetics and Kinetics of Cooperative Cofilin–Actin Filament Interactions. Journal Of Molecular Biology 2006, 361: 257-267. PMID: 16843490, DOI: 10.1016/j.jmb.2006.06.019.
- Thermodynamics of Nucleotide Binding to Actomyosin V and VI: A Positive Heat Capacity Change Accompanies Strong ADP Binding †Robblee JP, Cao W, Henn A, Hannemann DE, De La Cruz EM. Thermodynamics of Nucleotide Binding to Actomyosin V and VI: A Positive Heat Capacity Change Accompanies Strong ADP Binding †. Biochemistry 2005, 44: 10238-10249. PMID: 16042401, DOI: 10.1021/bi050232g.
- Magnesium, ADP, and Actin Binding Linkage of Myosin V: Evidence for Multiple Myosin V−ADP and Actomyosin V−ADP States †Hannemann DE, Cao W, Olivares AO, Robblee JP, De La Cruz EM. Magnesium, ADP, and Actin Binding Linkage of Myosin V: Evidence for Multiple Myosin V−ADP and Actomyosin V−ADP States †. Biochemistry 2005, 44: 8826-8840. PMID: 15952789, DOI: 10.1021/bi0473509.
- Two-color pump-probe laser spectroscopy instrument with picosecond time-resolved electronic delay and extended scan rangeREVIEW OF SCIENTIFIC INSTRUMENTS, 76, 114301-114308 (2005)
- Water Penetration and Binding to Ferric Myoglobin †Cao W, Christian J, Champion P, Rosca F, Sage J. Water Penetration and Binding to Ferric Myoglobin †. Biochemistry 2001, 40: 5728-5737. PMID: 11341838, DOI: 10.1021/bi010067e.
- Femtosecond Multicolor Pump-Probe Spectroscopy of Ferrous Cytochrome cJ. Phys. Chem. B 2000, 104, 10789-10801
- Energy-density correlation function of the Ising model in small magnetic fields for T< T c@article{PhysRevB.38.9152, title = {Energy-density correlation function of the Ising model in small magnetic fields for T<${T}_{c}$}, author = {Yan, Mu-Lin and Cao, Wen-Xiang}, journal = {Phys. Rev. B}, volume = {38}, issue = {13}, pages = {9152--9159}, numpages = {0}, year = {1988}, month = {Nov}, publisher = {American Physical Society}, doi = {10.1103/PhysRevB.38.9152}, url = {https://link.aps.org/doi/10.1103/PhysRevB.38.9152} }