Vincent Pieribone, PhD, BA
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Research Summary
Dr Pieribone is developing genetically encoded fluorescent probes of membrane electrical potential. These probes allow one to use optical instruments (microscopes) to monitor the electrical activity of neurons. Such an approach is less invasive, allows study of identified cell types over large regions of the cortical surface. The laboratory has also engineered miniature imaging systems that can be head mounted on mammels and allow mobile recording of neuronal activity. These types of studies will allow a better understanding of the neuronal networks that encode information in the central nervous system.
Specialized Terms: Neurophysiology; Neurotransmission; Voltage and calcium imaging; Sensory physiology; Drug development; Coral biology; Fluorescent proteins
Extensive Research Description
My published research has spanned 26 years (beginning with a Science paper published in 1986 while I was an undergraduate), produced >100 publications in peer-reviewed journals and I co-authored a well received (Nature, Technology or Mixed Signals Blog, CBE Life Science Education, Oceanography, Development, Yale Scientific and Nature Cell Biology), original peer-reviewed lay scientific book (“Aglow in the dark: the revolutionary science of biofluorescence” Harvard Press). My research has spanned a number of scientific areas: catecholamine anatomy and physiology, neuropeptide anatomy and physiology, adrenoceptor distribution in the central nervous system, serotonergic anatomy, the physiology and biochemistry of synaptic transmission, development of genetically-encoded voltage probes, identification of fluorescent proteins in marine animals, and information content in barrel cortex voltage-dye imaging. My work has received some 8,900 citations, an average of 58 per publication and 181 per year since 1986 (h-index of 47).
As an undergraduate and graduate student I published a series of papers on the afferent control of the locus coeruleus. These publications developed a novel and still held, concept that control of the brain’s main norepinephrine system largely resided in the hind brain. This concept realigned the fields perception of what factors controlled this important brain system. The locus coeruleus/norepinephrine system is involved in the actions of a number of widely used psychopharmacological interventions for depression, mania and schizophrenia.
As a Fogarty and National Science Foundation Fellow at the Nobel Institute of Neurophysiology in Stockholm Sweden, I published a series of studies on the anatomical distribution of the newly cloned adrenoceptors. These studies were the first to accurately identify which cells in the brain (and rest of the body) expressed which adrenoceptors. Previous receptor binding studies could not identify which cells or which cellular elements produced the labeled receptors and the specificity of the labeling was always questionable. These studies remain the most complete mapping of adrenoceptor subtypes in the brain (and other organs). While in Sweden I also examined the neurophysiologic response of monoaminergic neurons to the neuropeptide galanin and nitrous oxide. These represent one of only a handful of studies examining the neurophysiologic effects of galanin.
At The Rockefeller University I developed an in situ experimental preparation of an isolated large synapse for use in biochemical studies of neurotransmitter release. My research focused on the role of synapsin and actin in neurotransmission. We established that acute removal of the synaptic vesicle protein, synapsin from an adult synapses causes little change in low frequency neurotransmitter release but highly impairs high frequency neurotransmitter release. These studies combined with corroborative electron microscopic data, strongly suggested that the role of the most prevalent synaptic vesicle protein known is to create a reserve of synaptic vesicles that can be rapidly mobilized during bouts of high activity. The loss of this control was later shown to produce seizures in adult mice and in humans. At The Pierce Laboratory/Yale University I continued my work on synaptic transmission and moved to study the role of actin and actin- binding proteins on synaptic ultrastructure and physiology. In a series of papers we identified a clear cut role for actin, synapsins and talin in the recycling and clustering of synaptic vesicles.
In 2001 I began a research project to develop a genetically-encoded voltage probe based on green fluorescent protein. Being able to record electrical activity in virtually any specific cell type non-invasively will revolutionize neuroscience. In 2002 we published one of three original proof-of-concept genetically-encoded voltage probes to be developed by the scientific community. Our probe was the first to show rapid on and off kinetics. This publication led to an NIH R21 grants to explore other probes. At that time we also began to look for new fluorescent proteins that would preform better in voltage probes. We have begun a program to clone fluorescent proteins from coral. Our voltage probe became a strong proof-of- concept for the production of such probes. We subsequently received a multicenter (Yale, Riken (Japan), U. Montana, U. Penn. and U.C. Berkeley) NIH U24 grant that involved analyzing a large protein space for improved probes. This grant resulted the discovery and publication from my laboratory in September 2012, of a major advance in optical recording of brain activity (Jin, Lei et al. “Single Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe.” Neuron 75.5 (2012): 779–785.) This was followed by the first ever fully optical neurophysiology study (Cao G, Platisa J, Pieribone VA, Raccuglia D, Kunst M, Nitabach MN. Genetically targeted optical electrophysiology in intact neural circuits. Cell 154: 904–913, 2013.). This work has received considerable attention in relevant media (BioTechniques, Yale Medicine, Yale Daily News, Science Daily, Scientific American, National Geographic, NIH News, Reddit).
My laboratory has also developed the first head-mountable, high speed, fluorescent microscope system for monitoring electrical activity using genetically-encoded sensors of voltage and calcium. This microscope is now being used in combination with the above mentioned probe to study wide-spread motor cortical activity in an awake behaving animal with high temporal resolution. We are developing a fully optical neural prosthesis capable of informing accurate positional information in realtime. These studies are aimed at eventual human implementation in paralyzed patients.
I have been intimately involved in the development and Phase II/III clinical testing of the neurosteroid ganaxolone and allopregnanolone (I am the author of clinical grant applications, a peer-review paper and clinical protocols). I have co-developed the three clinical trials for these compounds in epilepsy ,post traumatic stress disorder and traumatic brain injury. I consider the development of neuropharmaceuticals a natural extension of my laboratory work at Yale University and the ultimate and most significant application of basic science to the betterment of human health.
I am currently the President and co-founder of Affinimark Technologies, Inc. which is developing a rapid point-of-care test for the presence of cerebrospinal fluid in bodily fluids leaked from the head. No such test exists and would improve patient care following traumatic head injury and sinus/ear surgery.
My scientific achievements extend well beyond my laboratory science. I am a Research Scientist at both the Mystic Aquarium and Center for Exploration in Mystic Connecticut and the American Museum of Natural History in New York City. I have co-curated a permanent exhibit at the Mystic Aquarium (http://www.mysticaquarium.org/animals-and-exhibits/exhibits/indoor/259-fluorescent-corals) and the most popular temporary exhibit to date at the American Museum of Natural History (- http://ez-www.amnh.org/creatures-of-light/ , National Science Foundation story ). For the American Museum of Natural History exhibit we received an National Science Foundation education grant. I have led several research expeditions to study and collect biofluorescent animals from coral reefs in Belize, British Virgin Islands, Malaysia, Australia, Israel, and the Solomon Islands. These have been funded by the National Institutes of Health, National Geographic Society, National Science Foundation, EarthWatch, and the American Museum of Natural History). Accounts of these trips have been documented in televised documentaries, radio interviews (Science and Society, National Public Radio, Studio 360, National Public Radio) and in web (National Geographic, Good News, Big Think, World Science Festival, Live Science) and print articles including The New York Times ( http://scientistatwork.blogs.nytimes.com/author/vincent-pieribone/). In 2010, we (along with my collaborator David Gruber) received an NSF Major Research Instrumentation grant to develop a remotely operated vehicle (ROV) to study deep sea fluorescent and bioluminescent animals.
Coauthors
Research Interests
Biomedical Engineering; Biotechnology; Cnidaria; Drug Therapy; Electrophysiology; Epilepsy; Neuropharmacology; Neurophysiology; Neurosciences; Optics and Photonics
Research Image
Selected Publications
- The covert world of fish biofluorescence: a phylogenetically widespread and phenotypically variable phenomenon.Sparks JS, Schelly RC, Smith WL, Davis MP, Tchernov D, Pieribone VA, Gruber DF. The covert world of fish biofluorescence: a phylogenetically widespread and phenotypically variable phenomenon. PloS One 2014, 9: e83259. PMID: 24421880, PMCID: PMC3885428, DOI: 10.1371/journal.pone.0083259.
- Genetically targeted optical electrophysiology in intact neural circuits.Cao G, Platisa J, Pieribone VA, Raccuglia D, Kunst M, Nitabach MN. Genetically targeted optical electrophysiology in intact neural circuits. Cell 2013, 154: 904-13. PMID: 23932121, PMCID: PMC3874294, DOI: 10.1016/j.cell.2013.07.027.
- Fluorescent protein voltage probes derived from ArcLight that respond to membrane voltage changes with fast kinetics.Han Z, Jin L, Platisa J, Cohen LB, Baker BJ, Pieribone VA. Fluorescent protein voltage probes derived from ArcLight that respond to membrane voltage changes with fast kinetics. PloS One 2013, 8: e81295. PMID: 24312287, PMCID: PMC3842285, DOI: 10.1371/journal.pone.0081295.
- A fluorescent, genetically-encoded voltage probe capable of resolving action potentials.Barnett L, Platisa J, Popovic M, Pieribone VA, Hughes T. A fluorescent, genetically-encoded voltage probe capable of resolving action potentials. PloS One 2012, 7: e43454. PMID: 22970127, PMCID: PMC3435330, DOI: 10.1371/journal.pone.0043454.
- Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe.Jin L, Han Z, Platisa J, Wooltorton JR, Cohen LB, Pieribone VA. Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe. Neuron 2012, 75: 779-85. PMID: 22958819, PMCID: PMC3439164, DOI: 10.1016/j.neuron.2012.06.040.
- Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics.Baker BJ, Jin L, Han Z, Cohen LB, Popovic M, Platisa J, Pieribone V. Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics. Journal Of Neuroscience Methods 2012, 208: 190-6. PMID: 22634212, PMCID: PMC3398169, DOI: 10.1016/j.jneumeth.2012.05.016.
- Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells.Jin L, Baker B, Mealer R, Cohen L, Pieribone V, Pralle A, Hughes T. Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells. Journal Of Neuroscience Methods 2011, 199: 1-9. PMID: 21497167, PMCID: PMC3281265, DOI: 10.1016/j.jneumeth.2011.03.028.
- Head-mountable high speed camera for optical neural recording.Park JH, Platisa J, Verhagen JV, Gautam SH, Osman A, Kim D, Pieribone VA, Culurciello E. Head-mountable high speed camera for optical neural recording. Journal Of Neuroscience Methods 2011, 201: 290-5. PMID: 21763348, PMCID: PMC3179772, DOI: 10.1016/j.jneumeth.2011.06.024.
- Effect of high velocity, large amplitude stimuli on the spread of depolarization in S1 "barrel" cortex.Davis DJ, Sachdev R, Pieribone VA. Effect of high velocity, large amplitude stimuli on the spread of depolarization in S1 "barrel" cortex. Somatosensory & Motor Research 2011, 28: 73-85. PMID: 22150170, PMCID: PMC3753103, DOI: 10.3109/08990220.2011.613177.
- A new bright green-emitting fluorescent protein--engineered monomeric and dimeric forms.Ilagan RP, Rhoades E, Gruber DF, Kao HT, Pieribone VA, Regan L. A new bright green-emitting fluorescent protein--engineered monomeric and dimeric forms. The FEBS Journal 2010, 277: 1967-78. PMID: 20345907, PMCID: PMC2855763, DOI: 10.1111/j.1742-4658.2010.07618.x.
- Novel internal regions of fluorescent proteins undergo divergent evolutionary patterns.Gruber DF, DeSalle R, Lienau EK, Tchernov D, Pieribone VA, Kao HT. Novel internal regions of fluorescent proteins undergo divergent evolutionary patterns. Molecular Biology And Evolution 2009, 26: 2841-8. PMID: 19770223, PMCID: PMC2775108, DOI: 10.1093/molbev/msp194.
- Genetically encoded fluorescent sensors of membrane potential.Baker BJ, Mutoh H, Dimitrov D, Akemann W, Perron A, Iwamoto Y, Jin L, Cohen LB, Isacoff EY, Pieribone VA, Hughes T, Knöpfel T. Genetically encoded fluorescent sensors of membrane potential. Brain Cell Biology 2008, 36: 53-67. PMID: 18679801, PMCID: PMC2775812, DOI: 10.1007/s11068-008-9026-7.
- Patterns of fluorescent protein expression in Scleractinian corals.Gruber DF, Kao HT, Janoschka S, Tsai J, Pieribone VA. Patterns of fluorescent protein expression in Scleractinian corals. The Biological Bulletin 2008, 215: 143-54. PMID: 18840775, DOI: 10.2307/25470695.
- Early involvement of synapsin III in neural progenitor cell development in the adult hippocampus.Kao HT, Li P, Chao HM, Janoschka S, Pham K, Feng J, Mcewen BS, Greengard P, Pieribone VA, Porton B. Early involvement of synapsin III in neural progenitor cell development in the adult hippocampus. The Journal Of Comparative Neurology 2008, 507: 1860-70. PMID: 18271024, DOI: 10.1002/cne.21643.
- Dynamic regulation of fluorescent proteins from a single species of coral.Kao HT, Sturgis S, DeSalle R, Tsai J, Davis D, Gruber DF, Pieribone VA. Dynamic regulation of fluorescent proteins from a single species of coral. Marine Biotechnology (New York, N.Y.) 2007, 9: 733-46. PMID: 17955294, DOI: 10.1007/s10126-007-9025-1.
- In vivo simultaneous tracing and Ca(2+) imaging of local neuronal circuits.Nagayama S, Zeng S, Xiong W, Fletcher ML, Masurkar AV, Davis DJ, Pieribone VA, Chen WR. In vivo simultaneous tracing and Ca(2+) imaging of local neuronal circuits. Neuron 2007, 53: 789-803. PMID: 17359915, PMCID: PMC1892750, DOI: 10.1016/j.neuron.2007.02.018.
- A genetically targetable fluorescent probe of channel gating with rapid kinetics.Ataka K, Pieribone VA. A genetically targetable fluorescent probe of channel gating with rapid kinetics. Biophysical Journal 2002, 82: 509-16. PMID: 11751337, PMCID: PMC1302490, DOI: 10.1016/S0006-3495(02)75415-5.
- Midbrain serotonergic neurons are central pH chemoreceptors.Severson CA, Wang W, Pieribone VA, Dohle CI, Richerson GB. Midbrain serotonergic neurons are central pH chemoreceptors. Nature Neuroscience 2003, 6: 1139-40. PMID: 14517544, DOI: 10.1038/nn1130.
- Genetically encoded fluorescent voltage indicators: are we there yet?Platisa J, Pieribone VA. Genetically encoded fluorescent voltage indicators: are we there yet? Current Opinion In Neurobiology 2018, 50: 146-153. PMID: 29501950, PMCID: PMC5984684, DOI: 10.1016/j.conb.2018.02.006.
- Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight.Platisa J, Vasan G, Yang A, Pieribone VA. Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight. ACS Chemical Neuroscience 2017, 8: 513-523. PMID: 28045247, PMCID: PMC5355904, DOI: 10.1021/acschemneuro.6b00234.
- Biofluorescence in Catsharks (Scyliorhinidae): Fundamental Description and Relevance for Elasmobranch Visual Ecology.Gruber DF, Loew ER, Deheyn DD, Akkaynak D, Gaffney JP, Smith WL, Davis MP, Stern JH, Pieribone VA, Sparks JS. Biofluorescence in Catsharks (Scyliorhinidae): Fundamental Description and Relevance for Elasmobranch Visual Ecology. Scientific Reports 2016, 6: 24751. PMID: 27109385, PMCID: PMC4843165, DOI: 10.1038/srep24751.
- A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth.Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P. A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neuroscience 2002, 5: 431-7. PMID: 11976703, DOI: 10.1038/nn840.
- Expression of synapsin III in nerve terminals and neurogenic regions of the adult brain.Pieribone VA, Porton B, Rendon B, Feng J, Greengard P, Kao HT. Expression of synapsin III in nerve terminals and neurogenic regions of the adult brain. The Journal Of Comparative Neurology 2002, 454: 105-14. PMID: 12412137, DOI: 10.1002/cne.10417.
- Impaired recycling of synaptic vesicles after acute perturbation of the presynaptic actin cytoskeleton.Shupliakov O, Bloom O, Gustafsson JS, Kjaerulff O, Low P, Tomilin N, Pieribone VA, Greengard P, Brodin L. Impaired recycling of synaptic vesicles after acute perturbation of the presynaptic actin cytoskeleton. Proceedings Of The National Academy Of Sciences Of The United States Of America 2002, 99: 14476-81. PMID: 12381791, PMCID: PMC137908, DOI: 10.1073/pnas.212381799.
- Colocalization of synapsin and actin during synaptic vesicle recycling.Bloom O, Evergren E, Tomilin N, Kjaerulff O, Löw P, Brodin L, Pieribone VA, Greengard P, Shupliakov O. Colocalization of synapsin and actin during synaptic vesicle recycling. The Journal Of Cell Biology 2003, 161: 737-47. PMID: 12756235, PMCID: PMC2199372, DOI: 10.1083/jcb.200212140.
- The role of actin in the regulation of dendritic spine morphology and bidirectional synaptic plasticity.Chen Y, Bourne J, Pieribone VA, Fitzsimonds RM. The role of actin in the regulation of dendritic spine morphology and bidirectional synaptic plasticity. Neuroreport 2004, 15: 829-32. PMID: 15073524, DOI: 10.1097/00001756-200404090-00018.
- A role for talin in presynaptic function.Morgan JR, Di Paolo G, Werner H, Shchedrina VA, Pypaert M, Pieribone VA, De Camilli P. A role for talin in presynaptic function. The Journal Of Cell Biology 2004, 167: 43-50. PMID: 15479735, PMCID: PMC2172527, DOI: 10.1083/jcb.200406020.
- Actin polymerization regulates clathrin coat maturation during early stages of synaptic vesicle recycling at lamprey synapses.Bourne J, Morgan JR, Pieribone VA. Actin polymerization regulates clathrin coat maturation during early stages of synaptic vesicle recycling at lamprey synapses. The Journal Of Comparative Neurology 2006, 497: 600-9. PMID: 16739194, DOI: 10.1002/cne.21006.
- Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells.Baker BJ, Lee H, Pieribone VA, Cohen LB, Isacoff EY, Knopfel T, Kosmidis EK. Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells. Journal Of Neuroscience Methods 2007, 161: 32-8. PMID: 17126911, DOI: 10.1016/j.jneumeth.2006.10.005.
- Clinical evaluation of ganaxolone in pediatric and adolescent patients with refractory epilepsy.Pieribone VA, Tsai J, Soufflet C, Rey E, Shaw K, Giller E, Dulac O. Clinical evaluation of ganaxolone in pediatric and adolescent patients with refractory epilepsy. Epilepsia 2007, 48: 1870-4. PMID: 17634060, DOI: 10.1111/j.1528-1167.2007.01182.x.
- Strict regulation of gene expression from a high-copy plasmid utilizing a dual vector system.Gruber DF, Pieribone VA, Porton B, Kao HT. Strict regulation of gene expression from a high-copy plasmid utilizing a dual vector system. Protein Expression And Purification 2008, 60: 53-7. PMID: 18434195, PMCID: PMC2442401, DOI: 10.1016/j.pep.2008.03.014.
- Design constraints for mobile, high-speed fluorescence brain imaging in awake animals.Osman A, Park JH, Dickensheets D, Platisa J, Culurciello E, Pieribone VA. Design constraints for mobile, high-speed fluorescence brain imaging in awake animals. IEEE Transactions On Biomedical Circuits And Systems 2012, 6: 446-53. PMID: 23853231, DOI: 10.1109/TBCAS.2012.2226174.
- Transcriptome deep-sequencing and clustering of expressed isoforms from Favia corals.Pooyaei Mehr SF, DeSalle R, Kao HT, Narechania A, Han Z, Tchernov D, Pieribone V, Gruber DF. Transcriptome deep-sequencing and clustering of expressed isoforms from Favia corals. BMC Genomics 2013, 14: 546. PMID: 23937070, PMCID: PMC3751062, DOI: 10.1186/1471-2164-14-546.
- Continuous Time Level Crossing Sampling ADC for Bio-Potential Recording Systems.Tang W, Osman A, Kim D, Goldstein B, Huang C, Martini B, Pieribone VA, Culurciello E. Continuous Time Level Crossing Sampling ADC for Bio-Potential Recording Systems. IEEE Transactions On Circuits And Systems. I, Regular Papers : A Publication Of The IEEE Circuits And Systems Society 2013, 60: 1407-1418. PMID: 24163640, PMCID: PMC3806520, DOI: 10.1109/TCSI.2012.2220464.
- Mechanistic studies of the genetically encoded fluorescent protein voltage probe ArcLight.Han Z, Jin L, Chen F, Loturco JJ, Cohen LB, Bondar A, Lazar J, Pieribone VA. Mechanistic studies of the genetically encoded fluorescent protein voltage probe ArcLight. PloS One 2014, 9: e113873. PMID: 25419571, PMCID: PMC4242678, DOI: 10.1371/journal.pone.0113873.
- Sensory determinants of behavioral dynamics in Drosophila thermotaxis.Klein M, Afonso B, Vonner AJ, Hernandez-Nunez L, Berck M, Tabone CJ, Kane EA, Pieribone VA, Nitabach MN, Cardona A, Zlatic M, Sprecher SG, Gershow M, Garrity PA, Samuel AD. Sensory determinants of behavioral dynamics in Drosophila thermotaxis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: E220-9. PMID: 25550513, PMCID: PMC4299240, DOI: 10.1073/pnas.1416212112.
- Transcriptome sequencing and annotation of the polychaete Hermodice carunculata (Annelida, Amphinomidae).Mehr S, Verdes A, DeSalle R, Sparks J, Pieribone V, Gruber DF. Transcriptome sequencing and annotation of the polychaete Hermodice carunculata (Annelida, Amphinomidae). BMC Genomics 2015, 16: 445. PMID: 26059236, PMCID: PMC4462082, DOI: 10.1186/s12864-015-1565-6.
- Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment.Gruber DF, Gaffney JP, Mehr S, DeSalle R, Sparks JS, Platisa J, Pieribone VA. Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment. PloS One 2015, 10: e0140972. PMID: 26561348, PMCID: PMC4641735, DOI: 10.1371/journal.pone.0140972.
- A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices.Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE. A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices. The Journal Of Neuroscience : The Official Journal Of The Society For Neuroscience 2016, 36: 2458-72. PMID: 26911693, PMCID: PMC4764664, DOI: 10.1523/JNEUROSCI.3484-15.2016.
- Dickkopf-related Protein 3 as a Sensitive and Specific Marker for Cerebrospinal Fluid Leaks.Michaelides EM, Kuang H, Pieribone VA. Dickkopf-related Protein 3 as a Sensitive and Specific Marker for Cerebrospinal Fluid Leaks. Otology & Neurotology : Official Publication Of The American Otological Society, American Neurotology Society [and] European Academy Of Otology And Neurotology 2016, 37: 299-303. PMID: 26837002, DOI: 10.1097/MAO.0000000000000954.
- Optimizing recruitment and retention of adolescents in ED research: Findings from concussion biomarker pilot study.Mbachu SN, Pieribone VA, Bechtel KA, McCarthy ML, Melnick ER. Optimizing recruitment and retention of adolescents in ED research: Findings from concussion biomarker pilot study. The American Journal Of Emergency Medicine 2018, 36: 884-887. PMID: 28918968, DOI: 10.1016/j.ajem.2017.09.014.
- Luciferin production and luciferase transcription in the bioluminescent copepod Metridia lucens.Tessler M, Gaffney JP, Crawford JM, Trautman E, Gujarati NA, Alatalo P, Pieribone VA, Gruber DF. Luciferin production and luciferase transcription in the bioluminescent copepod Metridia lucens. PeerJ 2018, 6: e5506. PMID: 30233994, PMCID: PMC6140675, DOI: 10.7717/peerj.5506.
- Fast, in vivo voltage imaging using a red fluorescent indicator.Kannan M, Vasan G, Huang C, Haziza S, Li JZ, Inan H, Schnitzer MJ, Pieribone VA. Fast, in vivo voltage imaging using a red fluorescent indicator. Nature Methods 2018, 15: 1108-1116. PMID: 30420685, PMCID: PMC6516062, DOI: 10.1038/s41592-018-0188-7.
- A Dexterous, Glove-Based Teleoperable Low-Power Soft Robotic Arm for Delicate Deep-Sea Biological Exploration.Phillips BT, Becker KP, Kurumaya S, Galloway KC, Whittredge G, Vogt DM, Teeple CB, Rosen MH, Pieribone VA, Gruber DF, Wood RJ. A Dexterous, Glove-Based Teleoperable Low-Power Soft Robotic Arm for Delicate Deep-Sea Biological Exploration. Scientific Reports 2018, 8: 14779. PMID: 30283051, PMCID: PMC6170437, DOI: 10.1038/s41598-018-33138-y.
- Optimizing Strategies for Developing Genetically Encoded Voltage Indicators.Kannan M, Vasan G, Pieribone VA. Optimizing Strategies for Developing Genetically Encoded Voltage Indicators. Frontiers In Cellular Neuroscience 2019, 13: 53. PMID: 30863283, PMCID: PMC6399427, DOI: 10.3389/fncel.2019.00053.
- Bright Green Biofluorescence in Sharks Derives from Bromo-Kynurenine Metabolism.Park HB, Lam YC, Gaffney JP, Weaver JC, Krivoshik SR, Hamchand R, Pieribone V, Gruber DF, Crawford JM. Bright Green Biofluorescence in Sharks Derives from Bromo-Kynurenine Metabolism. IScience 2019, 19: 1291-1336. PMID: 31402257, PMCID: PMC6831821, DOI: 10.1016/j.isci.2019.07.019.
- Spatiotemporal dynamics of odor responses in the lateral and dorsal olfactory bulb.Baker KL, Vasan G, Gumaste A, Pieribone VA, Verhagen JV. Spatiotemporal dynamics of odor responses in the lateral and dorsal olfactory bulb. PLoS Biology 2019, 17: e3000409. PMID: 31532763, PMCID: PMC6768483, DOI: 10.1371/journal.pbio.3000409.
- Bioluminescent flashes drive nighttime schooling behavior and synchronized swimming dynamics in flashlight fish.Gruber DF, Phillips BT, O'Brien R, Boominathan V, Veeraraghavan A, Vasan G, O'Brien P, Pieribone VA, Sparks JS. Bioluminescent flashes drive nighttime schooling behavior and synchronized swimming dynamics in flashlight fish. PloS One 2019, 14: e0219852. PMID: 31412054, PMCID: PMC6693688, DOI: 10.1371/journal.pone.0219852.
- Galanin message-associated peptide (GMAP)- and galanin-like immunoreactivities: overlapping and differential distributions in the rat.Hökfelt T, Aman K, Arvidsson U, Bedecs K, Ceccatelli S, Hulting AL, Langel U, Meister B, Pieribone V, Bartfai T. Galanin message-associated peptide (GMAP)- and galanin-like immunoreactivities: overlapping and differential distributions in the rat. Neuroscience Letters 1992, 142: 139-42. PMID: 1280789, DOI: 10.1016/0304-3940(92)90358-e.
- Serotonin-, substance P- and glutamate/aspartate-like immunoreactivities in medullo-spinal pathways of rat and primate.Nicholas AP, Pieribone VA, Arvidsson U, Hökfelt T. Serotonin-, substance P- and glutamate/aspartate-like immunoreactivities in medullo-spinal pathways of rat and primate. Neuroscience 1992, 48: 545-59. PMID: 1376453, DOI: 10.1016/0306-4522(92)90401-m.
- Differential expression of mRNAs for neuropeptide Y-related peptides in rat nervous tissues: possible evolutionary conservation.Pieribone VA, Brodin L, Friberg K, Dahlstrand J, Söderberg C, Larhammar D, Hökfelt T. Differential expression of mRNAs for neuropeptide Y-related peptides in rat nervous tissues: possible evolutionary conservation. The Journal Of Neuroscience : The Official Journal Of The Society For Neuroscience 1992, 12: 3361-71. PMID: 1527583, PMCID: PMC6575750.
- Calcitonin gene-related peptide in the brain, spinal cord, and some peripheral systems.Hökfelt T, Arvidsson U, Ceccatelli S, Cortés R, Cullheim S, Dagerlind A, Johnson H, Orazzo C, Piehl F, Pieribone V. Calcitonin gene-related peptide in the brain, spinal cord, and some peripheral systems. Annals Of The New York Academy Of Sciences 1992, 657: 119-34. PMID: 1637079, DOI: 10.1111/j.1749-6632.1992.tb22762.x.
- Afferent regulation of locus coeruleus neurons: anatomy, physiology and pharmacology.Aston-Jones G, Shipley MT, Chouvet G, Ennis M, van Bockstaele E, Pieribone V, Shiekhattar R, Akaoka H, Drolet G, Astier B. Afferent regulation of locus coeruleus neurons: anatomy, physiology and pharmacology. Progress In Brain Research 1991, 88: 47-75. PMID: 1687622, DOI: 10.1016/s0079-6123(08)63799-1.
- Adrenergic innervation of the rat nucleus locus coeruleus arises predominantly from the C1 adrenergic cell group in the rostral medulla.Pieribone VA, Aston-Jones G. Adrenergic innervation of the rat nucleus locus coeruleus arises predominantly from the C1 adrenergic cell group in the rostral medulla. Neuroscience 1991, 41: 525-42. PMID: 1714551, DOI: 10.1016/0306-4522(91)90346-p.
- Subregions of the periaqueductal gray topographically innervate the rostral ventral medulla in the rat.Van Bockstaele EJ, Aston-Jones G, Pieribone VA, Ennis M, Shipley MT. Subregions of the periaqueductal gray topographically innervate the rostral ventral medulla in the rat. The Journal Of Comparative Neurology 1991, 309: 305-27. PMID: 1717516, DOI: 10.1002/cne.903090303.
- Anatomical evidence for multiple pathways leading from the rostral ventrolateral medulla (nucleus paragigantocellularis) to the locus coeruleus in rat.Astier B, Van Bockstaele EJ, Aston-Jones G, Pieribone VA. Anatomical evidence for multiple pathways leading from the rostral ventrolateral medulla (nucleus paragigantocellularis) to the locus coeruleus in rat. Neuroscience Letters 1990, 118: 141-6. PMID: 2274260, DOI: 10.1016/0304-3940(90)90612-d.
- Diverse afferents converge on the nucleus paragigantocellularis in the rat ventrolateral medulla: retrograde and anterograde tracing studies.Van Bockstaele EJ, Pieribone VA, Aston-Jones G. Diverse afferents converge on the nucleus paragigantocellularis in the rat ventrolateral medulla: retrograde and anterograde tracing studies. The Journal Of Comparative Neurology 1989, 290: 561-84. PMID: 2482306, DOI: 10.1002/cne.902900410.
- The iontophoretic application of Fluoro-Gold for the study of afferents to deep brain nuclei.Pieribone VA, Aston-Jones G. The iontophoretic application of Fluoro-Gold for the study of afferents to deep brain nuclei. Brain Research 1988, 475: 259-71. PMID: 3214735, DOI: 10.1016/0006-8993(88)90614-2.
- Adrenergic and non-adrenergic neurons in the C1 and C3 areas project to locus coeruleus: a fluorescent double labeling study.Pieribone VA, Aston-Jones G, Bohn MC. Adrenergic and non-adrenergic neurons in the C1 and C3 areas project to locus coeruleus: a fluorescent double labeling study. Neuroscience Letters 1988, 85: 297-303. PMID: 3362420, DOI: 10.1016/0304-3940(88)90582-4.
- The brain nucleus locus coeruleus: restricted afferent control of a broad efferent network.Aston-Jones G, Ennis M, Pieribone VA, Nickell WT, Shipley MT. The brain nucleus locus coeruleus: restricted afferent control of a broad efferent network. Science (New York, N.Y.) 1986, 234: 734-7. PMID: 3775363, DOI: 10.1126/science.3775363.
- Immunohistochemical analysis of the relation between 5-hydroxytryptamine- and neuropeptide-immunoreactive elements in the spinal cord of an amphibian (Xenopus laevis).Pieribone VA, Brodin L, Hökfelt T. Immunohistochemical analysis of the relation between 5-hydroxytryptamine- and neuropeptide-immunoreactive elements in the spinal cord of an amphibian (Xenopus laevis). The Journal Of Comparative Neurology 1994, 341: 492-506. PMID: 7515401, DOI: 10.1002/cne.903410406.
- A functional role for nitric oxide in locus coeruleus: immunohistochemical and electrophysiological studies.Xu ZQ, Pieribone VA, Zhang X, Grillner S, Hökfelt T. A functional role for nitric oxide in locus coeruleus: immunohistochemical and electrophysiological studies. Experimental Brain Research 1994, 98: 75-83. PMID: 7516892, DOI: 10.1007/BF00229111.
- Galanin induces a hyperpolarization of norepinephrine-containing locus coeruleus neurons in the brainstem slice.Pieribone VA, Xu ZQ, Zhang X, Grillner S, Bartfai T, Hökfelt T. Galanin induces a hyperpolarization of norepinephrine-containing locus coeruleus neurons in the brainstem slice. Neuroscience 1995, 64: 861-74. PMID: 7538638, DOI: 10.1016/0306-4522(94)00450-j.
- Synaptic vesicle depletion in reticulospinal axons is reduced by 5-hydroxytryptamine: direct evidence for presynaptic modulation of glutamatergic transmission.Shupliakov O, Pieribone VA, Gad H, Brodin L. Synaptic vesicle depletion in reticulospinal axons is reduced by 5-hydroxytryptamine: direct evidence for presynaptic modulation of glutamatergic transmission. The European Journal Of Neuroscience 1995, 7: 1111-6. PMID: 7613617, DOI: 10.1111/j.1460-9568.1995.tb01099.x.
- Distinct pools of synaptic vesicles in neurotransmitter release.Pieribone VA, Shupliakov O, Brodin L, Hilfiker-Rothenfluh S, Czernik AJ, Greengard P. Distinct pools of synaptic vesicles in neurotransmitter release. Nature 1995, 375: 493-7. PMID: 7777058, DOI: 10.1038/375493a0.
- The reticulospinal glutamate synapse in lamprey: plasticity and presynaptic variability.Brodin L, Shupliakov O, Pieribone VA, Hellgren J, Hill RH. The reticulospinal glutamate synapse in lamprey: plasticity and presynaptic variability. Journal Of Neurophysiology 1994, 72: 592-604. PMID: 7983521, DOI: 10.1152/jn.1994.72.2.592.
- Distribution of alpha 1 adrenoceptors in rat brain revealed by in situ hybridization experiments utilizing subtype-specific probes.Pieribone VA, Nicholas AP, Dagerlind A, Hökfelt T. Distribution of alpha 1 adrenoceptors in rat brain revealed by in situ hybridization experiments utilizing subtype-specific probes. The Journal Of Neuroscience : The Official Journal Of The Society For Neuroscience 1994, 14: 4252-68. PMID: 8027777, PMCID: PMC6577046.
- Neuropeptide role of both peptide YY and neuropeptide Y in vertebrates suggested by abundant expression of their mRNAs in a cyclostome brain.Söderberg C, Pieribone VA, Dahlstrand J, Brodin L, Larhammar D. Neuropeptide role of both peptide YY and neuropeptide Y in vertebrates suggested by abundant expression of their mRNAs in a cyclostome brain. Journal Of Neuroscience Research 1994, 37: 633-40. PMID: 8028041, DOI: 10.1002/jnr.490370510.
- Evolutionary conservation of synaptosome-associated protein 25 kDa (SNAP-25) shown by Drosophila and Torpedo cDNA clones.Risinger C, Blomqvist AG, Lundell I, Lambertsson A, Nässel D, Pieribone VA, Brodin L, Larhammar D. Evolutionary conservation of synaptosome-associated protein 25 kDa (SNAP-25) shown by Drosophila and Torpedo cDNA clones. The Journal Of Biological Chemistry 1993, 268: 24408-14. PMID: 8226991.
- Cellular localization of messenger RNA for beta-1 and beta-2 adrenergic receptors in rat brain: an in situ hybridization study.Nicholas AP, Pieribone VA, Hökfelt T. Cellular localization of messenger RNA for beta-1 and beta-2 adrenergic receptors in rat brain: an in situ hybridization study. Neuroscience 1993, 56: 1023-39. PMID: 8284033, DOI: 10.1016/0306-4522(93)90148-9.
- Distributions of mRNAs for alpha-2 adrenergic receptor subtypes in rat brain: an in situ hybridization study.Nicholas AP, Pieribone V, Hökfelt T. Distributions of mRNAs for alpha-2 adrenergic receptor subtypes in rat brain: an in situ hybridization study. The Journal Of Comparative Neurology 1993, 328: 575-94. PMID: 8381444, DOI: 10.1002/cne.903280409.
- CGRP-like immunoreactivity in A11 dopamine neurons projecting to the spinal cord and a note on CGRP-CCK cross-reactivity.Orazzo C, Pieribone VA, Ceccatelli S, Terenius L, Hökfelt T. CGRP-like immunoreactivity in A11 dopamine neurons projecting to the spinal cord and a note on CGRP-CCK cross-reactivity. Brain Research 1993, 600: 39-48. PMID: 8422589, DOI: 10.1016/0006-8993(93)90399-8.
- Peptidergic neurons in the vertebrate spinal cord: evolutionary trends.Brodin L, Söderberg C, Pieribone V, Larhammar D. Peptidergic neurons in the vertebrate spinal cord: evolutionary trends. Progress In Brain Research 1995, 104: 61-74. PMID: 8552784, DOI: 10.1016/s0079-6123(08)61784-7.
- The distribution and significance of CNS adrenoceptors examined with in situ hybridization.Nicholas AP, Hökfelt T, Pieribone VA. The distribution and significance of CNS adrenoceptors examined with in situ hybridization. Trends In Pharmacological Sciences 1996, 17: 245-55. PMID: 8756183, DOI: 10.1016/0165-6147(96)10022-5.
- Presynaptic mechanisms in central synaptic transmission: 'biochemistry' of an intact glutamatergic synapse.Shupliakov O, Pieribone VA, Gad H, Brodin L. Presynaptic mechanisms in central synaptic transmission: 'biochemistry' of an intact glutamatergic synapse. Acta Physiologica Scandinavica 1996, 157: 369-79. PMID: 8830897, DOI: 10.1046/j.1365-201X.1996.31251000.x.
- Sustained neurotransmitter release: new molecular clues.Brodin L, Löw P, Gad H, Gustafsson J, Pieribone VA, Shupliakov O. Sustained neurotransmitter release: new molecular clues. The European Journal Of Neuroscience 1997, 9: 2503-11. PMID: 9517455, DOI: 10.1111/j.1460-9568.1997.tb01679.x.
- Regulation of iron metabolism in the sanguivore lamprey Lampetra fluviatilis--molecular cloning of two ferritin subunits and two iron-regulatory proteins (IRP) reveals evolutionary conservation of the iron-regulatory element (IRE)/IRP regulatory system.Andersen O, Pantopoulos K, Kao HT, Muckenthaler M, Youson JH, Pieribone V. Regulation of iron metabolism in the sanguivore lamprey Lampetra fluviatilis--molecular cloning of two ferritin subunits and two iron-regulatory proteins (IRP) reveals evolutionary conservation of the iron-regulatory element (IRE)/IRP regulatory system. European Journal Of Biochemistry / FEBS 1998, 254: 223-9. PMID: 9660174, DOI: 10.1046/j.1432-1327.1998.2540223.x.
- Galanin-5-hydroxytryptamine interactions: electrophysiological, immunohistochemical and in situ hybridization studies on rat dorsal raphe neurons with a note on galanin R1 and R2 receptors.Xu ZQ, Zhang X, Pieribone VA, Grillner S, Hökfelt T. Galanin-5-hydroxytryptamine interactions: electrophysiological, immunohistochemical and in situ hybridization studies on rat dorsal raphe neurons with a note on galanin R1 and R2 receptors. Neuroscience 1998, 87: 79-94. PMID: 9722143, DOI: 10.1016/s0306-4522(98)00151-1.
- Electrophysiologic effects of galanin on neurons of the central nervous system.Pieribone VA, Xu ZQ, Zhang X, Hökfelt T. Electrophysiologic effects of galanin on neurons of the central nervous system. Annals Of The New York Academy Of Sciences 1998, 863: 264-73. PMID: 9928177, DOI: 10.1111/j.1749-6632.1998.tb10701.x.
- Synapsins as regulators of neurotransmitter release.Hilfiker S, Pieribone VA, Czernik AJ, Kao HT, Augustine GJ, Greengard P. Synapsins as regulators of neurotransmitter release. Philosophical Transactions Of The Royal Society Of London. Series B, Biological Sciences 1999, 354: 269-79. PMID: 10212475, PMCID: PMC1692497, DOI: 10.1098/rstb.1999.0378.
- Regulation of synaptotagmin I phosphorylation by multiple protein kinases.Hilfiker S, Pieribone VA, Nordstedt C, Greengard P, Czernik AJ. Regulation of synaptotagmin I phosphorylation by multiple protein kinases. Journal Of Neurochemistry 1999, 73: 921-32. PMID: 10461881, DOI: 10.1046/j.1471-4159.1999.0730921.x.
- Molecular evolution of the synapsin gene family.Kao HT, Porton B, Hilfiker S, Stefani G, Pieribone VA, DeSalle R, Greengard P. Molecular evolution of the synapsin gene family. The Journal Of Experimental Zoology 1999, 285: 360-77. PMID: 10578110.
- Inhibition of neurotransmitter release in the lamprey reticulospinal synapse by antibody-mediated disruption of SNAP-25 function.Löw P, Norlin T, Risinger C, Larhammar D, Pieribone VA, Shupliakov O, Brodin L. Inhibition of neurotransmitter release in the lamprey reticulospinal synapse by antibody-mediated disruption of SNAP-25 function. European Journal Of Cell Biology 1999, 78: 787-93. PMID: 10604655, DOI: 10.1016/S0171-9335(99)80029-6.
- Multiple messengers in descending serotonin neurons: localization and functional implications.Hökfelt T, Arvidsson U, Cullheim S, Millhorn D, Nicholas AP, Pieribone V, Seroogy K, Ulfhake B. Multiple messengers in descending serotonin neurons: localization and functional implications. Journal Of Chemical Neuroanatomy 2000, 18: 75-86. PMID: 10708921, DOI: 10.1016/s0891-0618(99)00037-x.
- Severe deficiencies in dopamine signaling in presymptomatic Huntington's disease mice.Bibb JA, Yan Z, Svenningsson P, Snyder GL, Pieribone VA, Horiuchi A, Nairn AC, Messer A, Greengard P. Severe deficiencies in dopamine signaling in presymptomatic Huntington's disease mice. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 6809-14. PMID: 10829080, PMCID: PMC18747, DOI: 10.1073/pnas.120166397.
- Overcoming resistance to reverse transcriptase inhibitors.Pieribone V. Overcoming resistance to reverse transcriptase inhibitors. GMHC Treatment Issues : The Gay Men's Health Crisis Newsletter Of Experimental AIDS Therapies 1995, 9: 8-9. PMID: 11362911.
- Chemosensitive serotonergic neurons are closely associated with large medullary arteries.Bradley SR, Pieribone VA, Wang W, Severson CA, Jacobs RA, Richerson GB. Chemosensitive serotonergic neurons are closely associated with large medullary arteries. Nature Neuroscience 2002, 5: 401-2. PMID: 11967547, DOI: 10.1038/nn848.
- Initial observations on the localization of mRNA for alpha and beta adrenergic receptors in brain and peripheral tissues of rat using in situ hybridization.Nicholas AP, Pieribone VA, Elde R, Hökfelt T. Initial observations on the localization of mRNA for alpha and beta adrenergic receptors in brain and peripheral tissues of rat using in situ hybridization. Molecular And Cellular Neurosciences 1991, 2: 344-50. PMID: 19912818, DOI: 10.1016/1044-7431(91)90065-v.
- In situ hybridization. A complementary method to radioligand-mediated autoradiography for localizing adrenergic, alpha-2 receptor-producing cells.Nicholas AP, Pieribone V, Dagerlind A, Meister B, Elde R, Hökfelt T. In situ hybridization. A complementary method to radioligand-mediated autoradiography for localizing adrenergic, alpha-2 receptor-producing cells. Annals Of The New York Academy Of Sciences 1995, 763: 222-42. PMID: 7677334, DOI: 10.1111/j.1749-6632.1995.tb32409.x.
- A putative chordate luciferase from a cosmopolitan tunicate indicates convergent bioluminescence evolution across phyla.Tessler M, Gaffney JP, Oliveira AG, Guarnaccia A, Dobi KC, Gujarati NA, Galbraith M, Mirza JD, Sparks JS, Pieribone VA, Wood RJ, Gruber DF. A putative chordate luciferase from a cosmopolitan tunicate indicates convergent bioluminescence evolution across phyla. Scientific Reports 2020, 10: 17724. PMID: 33082360, PMCID: PMC7576829, DOI: 10.1038/s41598-020-73446-w.
- Voltage imaging in the olfactory bulb using transgenic mouse lines expressing the genetically encoded voltage indicator ArcLight.Platisa J, Zeng H, Madisen L, Cohen LB, Pieribone VA, Storace DA. Voltage imaging in the olfactory bulb using transgenic mouse lines expressing the genetically encoded voltage indicator ArcLight. Scientific Reports 2022, 12: 1875. PMID: 35115567, PMCID: PMC8813909, DOI: 10.1038/s41598-021-04482-3.