Research & Publications
My research focuses on translational studies of primary human cells in disease susceptibility and divergent clinical outcomes. The focus of our lab’s research is on innate immunity, specifically the interaction of macrophages, neutrophils, and dendritic cells with pathogens. We study the effect of aging on immune responses in large cohort study of human subjects, and show dysregulation of antiviral immunity in aging. We have published several methods using novel emerging technology to advance translational studies. In collaborative studies, we evaluate immune responses broadly in asthma, dengue virus, hepatitis C virus, humanized mice, leptospirosis, and Zika virus infections.
Our recent work employs CyTOF, a mass-spec based cell analyzer which provides multi-dimensional single cell data in suspension or in tissue sections. CyTOF improves on fluorescent flow cytometry by allowing detection of a greater number of markers per sample (~50) essentially without background signals or overlap between signals. I direct Yale's CyTOF Facility which houses two Helios instruments, one for cells in suspension and one dedicated to the Imaging Mass Cytometer (IMC) and I work with investigators to facilitate use of this novel platform.
Specialized Terms: Innate Immunity; Macrophage; Neutrophil; Aging; West Nile Virus
Extensive Research Description
Immune profiling; Single cell mass cytometry
Aging; Immune System; Immunity, Innate; Immune System Diseases; Macrophages; Neutrophils; Pathology; Public Health; Rheumatology; Virus Diseases; West Nile virus
Public Health Interests