Priscilla Dannies, PhD
Professor Emeritus of Pharmacology
Research & Publications
Biography
Research Summary
A major unanswered question in endocrinology is how protein hormones are concentrated into secretory granules. We have developed a model system of rat pituitary tumor cells, GH4C1 cells, which have few secretory granules, but can be induced to accumulate many and to store prolactin. We have identified mutants of human prolactin and human growth hormones that can not be stored themselves, and that interfere with the storage or stability of wild-type hormones.
We are investigating why these mutants behave as they do by physical techniques in solution and biochemical methods in cells. Dominant-negative mutants for secretion of hormones have been found in people deficient in growth hormone, vasopressin and amylin. Understanding how mutants of prolactin and growth hormone interfere with synthesis and storage or normal hormones will lead to understanding the mechanisms of these deficiencies as well as answering a basic question in endocrinology.
Extensive Research Description
A major unanswered question in endocrinology is how protein hormones are concentrated into secretory granules. We have developed a model system of rat pituitary tumor cells, GH4C1 cells, which have few secretory granules, but can be induced to accumulate many and to store prolactin. We have identified mutants of human prolactin and human growth hormones that can not be stored themselves, and that interfere with the storage or stability of wild-type hormones.
We are investigating why these mutants behave as they do by physical techniques in solution and biochemical methods in cells. Dominant-negative mutants for secretion of hormones have been found in people deficient in growth hormone, vasopressin and amylin. Understanding how mutants of prolactin and growth hormone interfere with synthesis and storage or normal hormones will lead to understanding the mechanisms of these eficiencies as well as answering a basic question in endocrinology.
We are investigating why these mutants behave as they do by physical techniques in solution and biochemical methods in cells. Dominant-negative mutants for secretion of hormones have been found in people deficient in growth hormone, vasopressin and amylin. Understanding how mutants of prolactin and growth hormone interfere with synthesis and storage or normal hormones will lead to understanding the mechanisms of these eficiencies as well as answering a basic question in endocrinology.
Coauthors
Research Interests
Endocrinology; Pharmacology; Prolactin; Vasopressins
Selected Publications
- Anti-tumor effects of adenovirus containing human growth hormone sequences in a mouse model of human ovarian cancerZhu Y, Fariña JB, Meshack S, Santoveña A, Patel S, Oliva A, Llabrés M, Hodsdon ME, Booth CJ, Dannies PS. Anti-tumor effects of adenovirus containing human growth hormone sequences in a mouse model of human ovarian cancer. Endocrine 2010, 37: 430-439. PMID: 20960164, DOI: 10.1007/s12020-010-9333-5.
- Pharmacokinetics analysis of sustained release hGH biodegradable implantable tablets using a mouse model of human ovarian cancerSantoveña A, Fariña JB, Llabrés M, Zhu Y, Dannies P. Pharmacokinetics analysis of sustained release hGH biodegradable implantable tablets using a mouse model of human ovarian cancer. International Journal Of Pharmaceutics 2010, 388: 175-180. PMID: 20060456, DOI: 10.1016/j.ijpharm.2009.12.054.
- Aggregation and Lack of Secretion of Most Newly Synthesized Proinsulin in Non-β-Cell LinesZhu YL, Abdo A, Gesmonde JF, Zawalich KC, Zawalich W, Dannies PS. Aggregation and Lack of Secretion of Most Newly Synthesized Proinsulin in Non-β-Cell Lines. Endocrinology 2004, 145: 3840-3849. PMID: 15117881, DOI: 10.1210/en.2003-1512.
- Is there structural specificity in the reversible protein aggregates that are stored in secretory granules?Keeler C, Hodsdon ME, Dannies PS. Is there structural specificity in the reversible protein aggregates that are stored in secretory granules? Journal Of Molecular Neuroscience 2004, 22: 43-49. PMID: 14742909, DOI: 10.1385/jmn:22:1-2:43.
- Peptide Hormones, Segregation MechanismDannies P. Peptide Hormones, Segregation Mechanism. 2004, 573-574. DOI: 10.1016/b0-12-475570-4/01000-3.
- Peptide Hormones, Subcellular StructureDannies P. Peptide Hormones, Subcellular Structure. 2004, 575-578. DOI: 10.1016/b0-12-475570-4/01001-5.
- Peptide Hormones, Regulated SecretionDannies P. Peptide Hormones, Regulated Secretion. 2004, 561-565. DOI: 10.1016/b0-12-475570-4/00998-7.
- Manipulating the Reversible Aggregation of Protein Hormones in Secretory GranulesDannies P. Manipulating the Reversible Aggregation of Protein Hormones in Secretory Granules. BioDrugs 2003, 17: 315-324. PMID: 14498762, DOI: 10.2165/00063030-200317050-00002.
- The Tertiary Structure and Backbone Dynamics of Human ProlactinKeeler C, Dannies PS, Hodsdon ME. The Tertiary Structure and Backbone Dynamics of Human Prolactin. Journal Of Molecular Biology 2003, 328: 1105-1121. PMID: 12729745, DOI: 10.1016/s0022-2836(03)00367-x.
- New GH-1 gene mutations: expanding the spectrum of causes of isolated growth hormone deficiency.Mullis PE, Deladoëy J, Dannies PS. New GH-1 gene mutations: expanding the spectrum of causes of isolated growth hormone deficiency. Journal Of Pediatric Endocrinology And Metabolism 2002, 15 Suppl 5: 1301-10. PMID: 12510984.
- Prolonged Retention after Aggregation into Secretory Granules of Human R183H-Growth Hormone (GH), a Mutant that Causes Autosomal Dominant GH Deficiency Type IIZhu YL, Conway-Campbell B, Waters MJ, Dannies PS. Prolonged Retention after Aggregation into Secretory Granules of Human R183H-Growth Hormone (GH), a Mutant that Causes Autosomal Dominant GH Deficiency Type II. Endocrinology 2002, 143: 4243-4248. PMID: 12399418, DOI: 10.1210/en.2002-220575.
- A serum prolactin-binding protein: implications for growth hormoneDannies P. A serum prolactin-binding protein: implications for growth hormone. Trends In Endocrinology And Metabolism 2001, 12: 427-428. PMID: 11701331, DOI: 10.1016/s1043-2760(01)00497-0.
- Misfolded growth hormone causes fragmentation of the Golgi apparatus and disrupts endoplasmic reticulum-to-Golgi traffic.Graves T, Patel S, Dannies P, Hinkle P. Misfolded growth hormone causes fragmentation of the Golgi apparatus and disrupts endoplasmic reticulum-to-Golgi traffic. Journal Of Cell Science 2001, 114: 3685-94. PMID: 11707520, DOI: 10.1242/jcs.114.20.3685.
- Concentrating hormones into secretory granules: layers of controlDannies P. Concentrating hormones into secretory granules: layers of control. Molecular And Cellular Endocrinology 2001, 177: 87-93. PMID: 11377824, DOI: 10.1016/s0303-7207(01)00437-3.
- Acquisition of Lubrol Insolubility, a Common Step for Growth Hormone and Prolactin in the Secretory Pathway of Neuroendocrine Cells*Lee M, Zhu Y, Chang J, Dannies P. Acquisition of Lubrol Insolubility, a Common Step for Growth Hormone and Prolactin in the Secretory Pathway of Neuroendocrine Cells*. Journal Of Biological Chemistry 2001, 276: 715-721. PMID: 11024038, DOI: 10.1074/jbc.m008530200.
- Accumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth FactorLee M, Zhu Y, Sun Z, Rhee H, Jeromin A, Roder J, Dannies P. Accumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor. Endocrinology 2000, 141: 3485-3492. PMID: 10965922, DOI: 10.1210/endo.141.9.7647.
- Argl83His, a New Mutational "Hot-Spot" in the Growth Hormone GeneWajnrajch M, Gertner J, Mullis P, Deladoey J, Cogan J, Lekhakula S, Kim S, Dannies P, Saenger P, Moshang T, Phillips J, Leibel R. Argl83His, a New Mutational "Hot-Spot" in the Growth Hormone Gene. International Journal On Disability And Human Development 2000, 1: 125-136. DOI: 10.1515/ijdhd.2000.1.3.125.
- Autosomal Dominant Growth Hormone (GH) Deficiency Type II: The Del32–71-GH Deletion Mutant Suppresses Secretion of Wild-Type GHLee M, Wajnrajch M, Kim S, Plotnick L, Wang J, Gertner J, Leibel R, Dannies P. Autosomal Dominant Growth Hormone (GH) Deficiency Type II: The Del32–71-GH Deletion Mutant Suppresses Secretion of Wild-Type GH. Endocrinology 2000, 141: 883-890. PMID: 10698162, DOI: 10.1210/endo.141.3.7380.
- Protein folding and deficiencies caused by dominant-negative mutants of hormonesDannies P. Protein folding and deficiencies caused by dominant-negative mutants of hormones. 2000, 58: 1-26. PMID: 10668393, DOI: 10.1016/s0083-6729(00)58019-4.
- Protein Hormone Storage in Secretory Granules: Mechanisms for Concentration and Sorting*Dannies P. Protein Hormone Storage in Secretory Granules: Mechanisms for Concentration and Sorting*. Endocrine Reviews 1999, 20: 3-21. PMID: 10047971, DOI: 10.1210/edrv.20.1.0354.
- Protein Hormone Storage in Secretory Granules: Mechanisms for Concentration and SortingDannies P. Protein Hormone Storage in Secretory Granules: Mechanisms for Concentration and Sorting. Endocrine Reviews 1999, 20: 3-21. DOI: 10.1210/er.20.1.3.
- Cell Biology of SecretionDannies P. Cell Biology of Secretion. 1998, 3-22. DOI: 10.1002/cphy.cp070101.
- Stabilization of the receptor protein tyrosine phosphatase-like protein ICA512 in GH4C1 cells upon treatment with estradiol, insulin, and epidermal growth factor.Lee M, Dirkx R, Solimena M, Dannies P. Stabilization of the receptor protein tyrosine phosphatase-like protein ICA512 in GH4C1 cells upon treatment with estradiol, insulin, and epidermal growth factor. Endocrinology 1998, 139: 2727-33. PMID: 9607778, DOI: 10.1210/endo.139.6.6039.
- Editorial: A New Releasing Factor? with Biotechnology and a Little Bit of LuckDannies P. Editorial: A New Releasing Factor? with Biotechnology and a Little Bit of Luck. Endocrinology 1997, 138: 5085-5086. PMID: 9389485, DOI: 10.1210/endo.138.12.5710.
- Inefficient secretion of human H27A-prolactin, a mutant that does not bind Zn2+.Sun Z, Lee M, Rhee H, Arrandale J, Dannies P. Inefficient secretion of human H27A-prolactin, a mutant that does not bind Zn2+. Endocrinology 1997, 11: 1544-51. PMID: 9280069, DOI: 10.1210/mend.11.10.0002.
- Properties of human prolactin (PRL) and H27A-PRL, a mutant that does not bind Zn++.Sun Z, Li P, Dannies P, Lee J. Properties of human prolactin (PRL) and H27A-PRL, a mutant that does not bind Zn++. Endocrinology 1996, 10: 265-71. PMID: 8833655, DOI: 10.1210/mend.10.3.8833655.
- KN-62, a calcium/calmodulin-dependent protein kinase II inhibitor, inhibits high potassium-stimulated prolactin secretion and intracellular calcium increases in anterior pituitary cellsCui Z, Hidaka H, Dannies P. KN-62, a calcium/calmodulin-dependent protein kinase II inhibitor, inhibits high potassium-stimulated prolactin secretion and intracellular calcium increases in anterior pituitary cells. Biochimica Et Biophysica Acta 1996, 1310: 343-347. PMID: 8599613, DOI: 10.1016/0167-4889(95)00170-0.
- Biological activity and immunological reactivity of human prolactin mutants.Rhee H, Sun Z, Kim S, Goffin V, Martial J, Dannies P. Biological activity and immunological reactivity of human prolactin mutants. Endocrinology 1995, 136: 4990-5. PMID: 7588233, DOI: 10.1210/endo.136.11.7588233.
- Lack of correlation of distribution of prolactin (PRL) charge isoforms with induction of PRL storage.Mastro R, Dannies P. Lack of correlation of distribution of prolactin (PRL) charge isoforms with induction of PRL storage. Endocrinology 1995, 136: 69-74. PMID: 7828559, DOI: 10.1210/endo.136.1.7828559.
- Inhibition of rat prolactin (PRL) storage by coexpression of human PRL.Arrandale J, Dannies P. Inhibition of rat prolactin (PRL) storage by coexpression of human PRL. Endocrinology 1994, 8: 1083-1090. PMID: 7997234, DOI: 10.1210/mend.8.8.7997234.
- Regulation of Parathyroid Hormone‐Related Peptide Gene Expression by Estrogen in GH4C1 Rat Pituitary Cells Has the Pattern of a Primary Response GeneHolt E, Lu C, Dreyer B, Dannies P, Broadus A. Regulation of Parathyroid Hormone‐Related Peptide Gene Expression by Estrogen in GH4C1 Rat Pituitary Cells Has the Pattern of a Primary Response Gene. Journal Of Neurochemistry 1994, 62: 1239-1246. PMID: 8133258, DOI: 10.1046/j.1471-4159.1994.62041239.x.
- 29 The 3-Acetylpyridine Model of Parkinsonism: Use of Cytochrome Oxidase Gene Expression as an Index of Alterations in Basal Ganglia FunctionDEUTCH A, GROBIN A, DANNIES P. 29 The 3-Acetylpyridine Model of Parkinsonism: Use of Cytochrome Oxidase Gene Expression as an Index of Alterations in Basal Ganglia Function. 1994, 443-464. DOI: 10.1016/b978-0-08-042276-3.50032-x.
- Rapid stimulation of rhodamine 123 efflux from multidrug-resistant KB cells by progesteroneJancis E, Hong-xing C, Carbone R, Hochberg R, Dannies P. Rapid stimulation of rhodamine 123 efflux from multidrug-resistant KB cells by progesterone. Biochemical Pharmacology 1993, 46: 1613-1619. PMID: 7902090, DOI: 10.1016/0006-2952(93)90331-p.
- Thyrotropin-releasing hormone-mediated Mn2+ entry in perifused rat anterior pituitary cellsCui Z, Dannies P. Thyrotropin-releasing hormone-mediated Mn2+ entry in perifused rat anterior pituitary cells. Biochemical Journal 1992, 283: 507-513. PMID: 1575695, PMCID: PMC1131064, DOI: 10.1042/bj2830507.
- Regulation of prolactin storageReaves B, Dannies P. Regulation of prolactin storage. Cell Biochemistry And Biophysics 1991, 19: 109. PMID: 1726881, DOI: 10.1007/bf02989884.
- Is a sorting signal necessary to package proteins into secretory granules?Reaves B, Dannies P. Is a sorting signal necessary to package proteins into secretory granules? Molecular And Cellular Endocrinology 1991, 79: c141-c145. PMID: 1936531, DOI: 10.1016/0303-7207(91)90085-7.
- Ca2+ channel agonists enhance thyrotropin-releasing hormone-induced inositol phosphates and prolactin secretionPachter J, Law G, Dannies P. Ca2+ channel agonists enhance thyrotropin-releasing hormone-induced inositol phosphates and prolactin secretion. European Journal Of Pharmacology 1991, 195: 373-379. PMID: 1714395, DOI: 10.1016/0014-2999(91)90478-9.
- Prolactin and insulin are targeted to the regulated pathway in GH4C1 cells, but their storage is differentially regulated.Reaves B, Van Itallie C, Moore H, Dannies P. Prolactin and insulin are targeted to the regulated pathway in GH4C1 cells, but their storage is differentially regulated. Endocrinology 1990, 4: 1017-26. PMID: 2284004, DOI: 10.1210/mend-4-7-1017.
- Thapsigargin, but not caffeine, blocks the ability of thyrotropin-releasing hormone to release Ca2+ from an intracellular store in GH4C1 pituitary cellsLaw G, Pachter J, Thastrup O, Hanley M, Dannies P. Thapsigargin, but not caffeine, blocks the ability of thyrotropin-releasing hormone to release Ca2+ from an intracellular store in GH4C1 pituitary cells. Biochemical Journal 1990, 267: 359-364. PMID: 1692207, PMCID: PMC1131296, DOI: 10.1042/bj2670359.
- Comparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell LineFlicker L, Reaves B, Das B, Dannies P. Comparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line. Neuroendocrinology 1990, 51: 658-663. PMID: 2114002, DOI: 10.1159/000125407.
- Ca2+ Transients Induced by Thyrotropin-Releasing Hormone Rapidly Lose Their Ability to Cause Release of ProlactinLaw G, Pachter J, Dannies P. Ca2+ Transients Induced by Thyrotropin-Releasing Hormone Rapidly Lose Their Ability to Cause Release of Prolactin. Endocrinology 1989, 3: 539-546. PMID: 2501668, DOI: 10.1210/mend-3-3-539.
- Ability of repetitive Ca2+ spikes to stimulate prolactin release is frequency dependentLaw G, Pachter J, Dannies P. Ability of repetitive Ca2+ spikes to stimulate prolactin release is frequency dependent. Biochemical And Biophysical Research Communications 1989, 158: 811-816. PMID: 2537637, DOI: 10.1016/0006-291x(89)92794-0.
- Expression of messenger ribonucleic acids encoding a parathyroid hormone-like peptide in normal human and animal tissues with abnormal expression in human parathyroid adenomas.Ikeda K, Weir E, Mangin M, Dannies P, Kinder B, Deftos L, Brown E, Broadus A. Expression of messenger ribonucleic acids encoding a parathyroid hormone-like peptide in normal human and animal tissues with abnormal expression in human parathyroid adenomas. Endocrinology 1988, 2: 1230-6. PMID: 3216862, DOI: 10.1210/mend-2-12-1230.
- TRH and BAY K 8644 synergistically stimulate prolactin release but not 45Ca2+ uptakePachter J, Law G, Dannies P. TRH and BAY K 8644 synergistically stimulate prolactin release but not 45Ca2+ uptake. American Journal Of Physiology 1988, 255: c633-c640. PMID: 2461093, DOI: 10.1152/ajpcell.1988.255.5.c633.
- Dopamine has no Effect on Thyrotropin-Releasing Hormone Mobilization of Calcium from Intracellular Stores in Rat Anterior Pituitary CellsLaw G, Pachter J, Dannies P. Dopamine has no Effect on Thyrotropin-Releasing Hormone Mobilization of Calcium from Intracellular Stores in Rat Anterior Pituitary Cells. Endocrinology 1988, 2: 966-972. PMID: 3141796, DOI: 10.1210/mend-2-10-966.
- Bombesin stimulates inositol polyphosphate production in GH4C1 pituitary tumor cells: Comparison with TRHPachter J, Law G, Dannies P. Bombesin stimulates inositol polyphosphate production in GH4C1 pituitary tumor cells: Comparison with TRH. Biochemical And Biophysical Research Communications 1988, 154: 654-659. PMID: 3135808, DOI: 10.1016/0006-291x(88)90189-1.
- Estrogen induces accumulation of the mitochondrial ribonucleic acid for subunit II of cytochrome oxidase in pituitary tumor cells.Van Itallie C, Dannies P. Estrogen induces accumulation of the mitochondrial ribonucleic acid for subunit II of cytochrome oxidase in pituitary tumor cells. Endocrinology 1988, 2: 332-7. PMID: 2837664, DOI: 10.1210/mend-2-4-332.
- Hormonal Induction of a Heterogeneous Population of Storage Granules in GH4C1 Pituitary Tumor CellsSCAMMELL J, BURRAGE T, DANNIES P. Hormonal Induction of a Heterogeneous Population of Storage Granules in GH4C1 Pituitary Tumor Cells. Annals Of The New York Academy Of Sciences 1987, 493: 66-69. DOI: 10.1111/j.1749-6632.1987.tb27182.x.
- Regulation of prolactin production and cell growth by estradiol: difference in sensitivity to estradiol occurs at level of messenger ribonucleic acid accumulation.AMARA J, ITALLIE C, DANNIES P. Regulation of prolactin production and cell growth by estradiol: difference in sensitivity to estradiol occurs at level of messenger ribonucleic acid accumulation. Endocrinology 1987, 120: 264-71. PMID: 3780563, DOI: 10.1210/endo-120-1-264.
- Antiestrogens are partial estrogen agonists for prolactin production in primary pituitary culturesMartinez-Campos A, Amara J, Dannies P. Antiestrogens are partial estrogen agonists for prolactin production in primary pituitary cultures. Molecular And Cellular Endocrinology 1986, 48: 127-133. PMID: 3803705, DOI: 10.1016/0303-7207(86)90035-3.
- Hormonal induction of secretory granules in a pituitary tumor cell line.SCAMMELL J, BURRAGE T, DANNIES P. Hormonal induction of secretory granules in a pituitary tumor cell line. Endocrinology 1986, 119: 1543-8. PMID: 3530721, DOI: 10.1210/endo-119-4-1543.
- Characterization of antiestrogen stimulation of cell number and prolactin productionAmara J, Dannies P. Characterization of antiestrogen stimulation of cell number and prolactin production. Molecular And Cellular Endocrinology 1986, 47: 183-189. PMID: 3758472, DOI: 10.1016/0303-7207(86)90111-5.
- Rat growth hormone gene expression is correlated with an unmethylated CGCG sequence near the transcription initiation site.Strobl J, Dannies P, Thompson E. Rat growth hormone gene expression is correlated with an unmethylated CGCG sequence near the transcription initiation site. Biochemistry 1986, 25: 3640-8. PMID: 3013292, DOI: 10.1021/bi00360a025.
- Dopamine inhibits prolactin release when cyclic adenosine 3',5'-monophosphate levels are elevated.DELBEKE P, SCAMMELL J, MARTINEZ-CAMPOS A, DANNIES P. Dopamine inhibits prolactin release when cyclic adenosine 3',5'-monophosphate levels are elevated. Endocrinology 1986, 118: 1271-7. PMID: 3004906, DOI: 10.1210/endo-118-4-1271.
- A possible differentiation of anterior pituitary cells in collagen gels into neuronsMartinez-Campos A, Dannies P. A possible differentiation of anterior pituitary cells in collagen gels into neurons. Cell And Tissue Research 1986, 244: 21-26. PMID: 2421915, DOI: 10.1007/bf00218377.
- Comparison of patterns of prolactin release in GH4C1 cells and primary pituitary culturesDelbeke D, Kojima I, Dannies P. Comparison of patterns of prolactin release in GH4C1 cells and primary pituitary cultures. Molecular And Cellular Endocrinology 1985, 43: 15-22. PMID: 3934015, DOI: 10.1016/0303-7207(85)90037-1.
- Stimulation of the adenosine 3',5'-monophosphate and the Ca2+ messenger systems together reverse dopaminergic inhibition of prolactin release.DELBEKE D, DANNIES P. Stimulation of the adenosine 3',5'-monophosphate and the Ca2+ messenger systems together reverse dopaminergic inhibition of prolactin release. Endocrinology 1985, 117: 439-46. PMID: 2990850, DOI: 10.1210/endo-117-2-439.
- Cysteamine causes reduction of prolactin monomers followed by aggregation in the rat pituitary gland.SCAMMELL J, BURRAGE T, EISENFELD A, DANNIES P. Cysteamine causes reduction of prolactin monomers followed by aggregation in the rat pituitary gland. Endocrinology 1985, 116: 2347-54. PMID: 3996317, DOI: 10.1210/endo-116-6-2347.
- CHAPTER 9 Control of Prolactin Production by EstrogenDannies P. CHAPTER 9 Control of Prolactin Production by Estrogen. 1985, 289-310. DOI: 10.1016/b978-0-12-452812-3.50013-0.
- Receptor biochemistry and methodology, volume I: Membranes, detergents and receptor solubilization; volume II: receptor purification procedures; volume III: Molecular and chemical characterization of membrane receptors Series editors: J. Craig Venter and Len C. Harrison, Alan R. Liss, Inc., 1984. £35.00, £26.00 and £40.00 (xii + 225 pages, xii + 171 pages and xiii + 293 pages) ISBN 0 845 13700X, 0 845 137018 and 0 845 13702 6Dannies P. Receptor biochemistry and methodology, volume I: Membranes, detergents and receptor solubilization; volume II: receptor purification procedures; volume III: Molecular and chemical characterization of membrane receptors Series editors: J. Craig Venter and Len C. Harrison, Alan R. Liss, Inc., 1984. £35.00, £26.00 and £40.00 (xii + 225 pages, xii + 171 pages and xiii + 293 pages) ISBN 0 845 13700X, 0 845 137018 and 0 845 13702 6. Trends In Pharmacological Sciences 1985, 6: 137-138. DOI: 10.1016/0165-6147(85)90060-4.
- ProlactinDannies P. Prolactin. 1985, 159-174. DOI: 10.1007/978-1-4684-7018-5_7.
- Unresponsiveness of GH cells to cyclo(histidyl-proline), a metabolite of thyrotropin releasing hormoneBattaini F, Dannies P, Peterkofsky A. Unresponsiveness of GH cells to cyclo(histidyl-proline), a metabolite of thyrotropin releasing hormone. Life Sciences 1984, 35: 2519-2527. PMID: 6096658, DOI: 10.1016/0024-3205(84)90438-7.
- Synergistic stimulation of prolactin release by phorbol ester, A23187 and forskolinDelbeke D, Kojima I, Dannies P, Rasmussen H. Synergistic stimulation of prolactin release by phorbol ester, A23187 and forskolin. Biochemical And Biophysical Research Communications 1984, 123: 735-741. PMID: 6091639, DOI: 10.1016/0006-291x(84)90291-2.
- Difference in calcium requirements for forskolin-induced release of prolactin from normal pituitary cells and GH4C1 cells in culture.DELBEKE D, SCAMMELI J, DANNIES P. Difference in calcium requirements for forskolin-induced release of prolactin from normal pituitary cells and GH4C1 cells in culture. Endocrinology 1984, 114: 1433-40. PMID: 6323146, DOI: 10.1210/endo-114-4-1433.
- Depletion of pituitary prolactin by cysteamine is due to loss of immunological activity.SCAMMELL J, DANNIES P. Depletion of pituitary prolactin by cysteamine is due to loss of immunological activity. Endocrinology 1984, 114: 712-6. PMID: 6697959, DOI: 10.1210/endo-114-3-712.
- Spiperone: evidence for uptake into secretory granules.Dannies P, Rudnick M, Fishkes H, Rudnick G. Spiperone: evidence for uptake into secretory granules. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 1867-1870. PMID: 6584920, PMCID: PMC345023, DOI: 10.1073/pnas.81.6.1867.
- The Anterior Pituitary GlandDannies P. The Anterior Pituitary Gland. The Yale Journal Of Biology And Medicine 1984, 57: 724-725. PMCID: PMC2589997.
- ESTROGEN EFFECTS ON PROLACTIN STORAGE AND CELL GROWTH IN GH CELLS**Supported by United States Public Health Services Grant HD-11487.DANNIES P, KIINO D, AMARA J. ESTROGEN EFFECTS ON PROLACTIN STORAGE AND CELL GROWTH IN GH CELLS**Supported by United States Public Health Services Grant HD-11487. 1984, 121-140. DOI: 10.1016/b978-0-12-490620-4.50014-x.
- 20K is bound with high affinity by one rat and one of two rabbit growth hormone receptors.HUGHES J, TOKUHIRO E, STEVEN J, SIMPSON A, FRIESEN H, Dannies P. 20K is bound with high affinity by one rat and one of two rabbit growth hormone receptors. Endocrinology 1983, 113: 1904-6. PMID: 6313333, DOI: 10.1210/endo-113-5-1904.
- Tamoxifen and 5-fluorouracil in breast cancer: cytotoxic synergism in vitro.Benz C, Cadman E, Gwin J, Wu T, Amara J, Eisenfeld A, Dannies P. Tamoxifen and 5-fluorouracil in breast cancer: cytotoxic synergism in vitro. Cancer Research 1983, 43: 5298-303. PMID: 6616464.
- Veratridine and ouabain stimulate calcium-dependent prolactin release.SCAMMELL J, DANNIES P. Veratridine and ouabain stimulate calcium-dependent prolactin release. Endocrinology 1983, 113: 1228-35. PMID: 6617570, DOI: 10.1210/endo-113-4-1228.
- Comparison of the effects of histamine H2-receptor antagonists on prolactin secretion in the rat.NETTI C, GUIDOBONO F, OLGIATI V, SIBILIA V, PECILE A, Dannies P. Comparison of the effects of histamine H2-receptor antagonists on prolactin secretion in the rat. Endocrinology 1983, 113: 412-4. PMID: 6305638, DOI: 10.1210/endo-113-1-412.
- Synthesis and biological activity of 5-fluoroimidazole-TRHLabroo V, Kirk K, Cohen L, Delbeke D, Dannies P. Synthesis and biological activity of 5-fluoroimidazole-TRH. Biochemical And Biophysical Research Communications 1983, 113: 581-585. PMID: 6409106, DOI: 10.1016/0006-291x(83)91765-5.
- 17 beta-Estradiol has a biphasic effect on gh cell growth.AMARA J, DANNIES P. 17 beta-Estradiol has a biphasic effect on gh cell growth. Endocrinology 1983, 112: 1141-3. PMID: 6822206, DOI: 10.1210/endo-112-3-1141.
- Prolactin: Multiple intracellular processing routes plus several potential mechanisms for regulationDannies P. Prolactin: Multiple intracellular processing routes plus several potential mechanisms for regulation. Biochemical Pharmacology 1982, 31: 2845-2849. PMID: 6291547, DOI: 10.1016/0006-2952(82)90253-2.
- Somatic cell hybridization of growth hormone-producing rat pituitary cells and mouse fibroblasts results in extinction of growth hormone expression via a defect in growth hormone RNA production.Strobl J, Dannies P, Thompson E. Somatic cell hybridization of growth hormone-producing rat pituitary cells and mouse fibroblasts results in extinction of growth hormone expression via a defect in growth hormone RNA production. Journal Of Biological Chemistry 1982, 257: 6588-6594. PMID: 6176584, DOI: 10.1016/s0021-9258(20)65183-3.
- Hormonal regulation of prolactin storage in a clonal strain of rat pituitary tumor cells.Kiino D, Dannies P. Hormonal regulation of prolactin storage in a clonal strain of rat pituitary tumor cells. The Yale Journal Of Biology And Medicine 1982, 55: 409-20. PMID: 6763814, PMCID: PMC2596570.
- Protein Degradation in Health and Disease. Ciba Foundation Symposium 75 (new series)Dannies P. Protein Degradation in Health and Disease. Ciba Foundation Symposium 75 (new series). The Yale Journal Of Biology And Medicine 1982, 55: 75-75. PMCID: PMC2595993.
- Molecular endocrinology, vol. 1 edited by I. MacIntyre and M. Szelke, Elsevier/North-Holland Biomedical Press, 1979. $58.50/Dfl. 120.00 (xvii + 437 pages) ISBN 0 444 80187 1Dannies P. Molecular endocrinology, vol. 1 edited by I. MacIntyre and M. Szelke, Elsevier/North-Holland Biomedical Press, 1979. $58.50/Dfl. 120.00 (xvii + 437 pages) ISBN 0 444 80187 1. Trends In Pharmacological Sciences 1982, 3: 126. DOI: 10.1016/0165-6147(82)91051-3.
- Histidyl-proline diketopiperazine: its biological role as a regulatory peptidePeterkofsky A, Battaini F, Koch Y, Takahara Y, Dannies P. Histidyl-proline diketopiperazine: its biological role as a regulatory peptide. Molecular And Cellular Biochemistry 1982, 42: 45-63. PMID: 6278283, DOI: 10.1007/bf00223538.
- Endocrine control of growth edited by William H. Daughaday, Elsevier, 1981. Dfl. 82.00 (xii + 275 pages) ISBN 0 444 00434 3Dannies P. Endocrine control of growth edited by William H. Daughaday, Elsevier, 1981. Dfl. 82.00 (xii + 275 pages) ISBN 0 444 00434 3. Trends In Pharmacological Sciences 1982, 3: 263-264. DOI: 10.1016/0165-6147(82)91123-3.
- 16 Mechanisms of Regulation of Prolactin ReleaseDANNIES P, TAM S. 16 Mechanisms of Regulation of Prolactin Release. 1982, 529-568. DOI: 10.1016/b978-0-12-185058-6.50023-x.
- Insulin and 17 beta-estradiol increase the intracellular prolactin content of GH4C1 cells.KIINO D, DANNIES P. Insulin and 17 beta-estradiol increase the intracellular prolactin content of GH4C1 cells. Endocrinology 1981, 109: 1264-9. PMID: 7026222, DOI: 10.1210/endo-109-4-1264.
- Muscarinic inhibition of prolactin production in cultures of rat pituitary cellsRudnick M, Dannies P. Muscarinic inhibition of prolactin production in cultures of rat pituitary cells. Biochemical And Biophysical Research Communications 1981, 101: 689-696. PMID: 7306103, DOI: 10.1016/0006-291x(81)91313-9.
- Dopaminergic inhibition of ionophore A23187-stimulated release of prolactin from rat anterior pituitary cells.Tam S, Dannies P. Dopaminergic inhibition of ionophore A23187-stimulated release of prolactin from rat anterior pituitary cells. Journal Of Biological Chemistry 1980, 255: 6595-6599. PMID: 6771274, DOI: 10.1016/s0021-9258(18)43611-3.
- Action of cholera toxin on hormone synthesis and release in GH cells: evidence that adenosine 3',5'-monophosphate does not mediate the decrease in growth hormone synthesis caused by thyrotropin-releasing hormone.DANNIES P, TASHJIAN A. Action of cholera toxin on hormone synthesis and release in GH cells: evidence that adenosine 3',5'-monophosphate does not mediate the decrease in growth hormone synthesis caused by thyrotropin-releasing hormone. Endocrinology 1980, 106: 1532-6. PMID: 6244936, DOI: 10.1210/endo-106-5-1532.
- Effects of estradiol on prolactin production and dihydroergocryptine-induced inhibition of prolactin production in primary cultures of rat pituitary cells.WEST B, DANNIES P. Effects of estradiol on prolactin production and dihydroergocryptine-induced inhibition of prolactin production in primary cultures of rat pituitary cells. Endocrinology 1980, 106: 1108-13. PMID: 6766855, DOI: 10.1210/endo-106-4-1108.
- 2-Bromo-alpha-ergocryptine causes degradation of prolactin in primary cultures of rat pituitary cells after chronic treatment.Dannies P, Rudnick M. 2-Bromo-alpha-ergocryptine causes degradation of prolactin in primary cultures of rat pituitary cells after chronic treatment. Journal Of Biological Chemistry 1980, 255: 2776-2781. PMID: 7358708, DOI: 10.1016/s0021-9258(19)85806-4.
- Differential ability of thyrotropin-releasing hormone and N3im-methyl-thyrotropin-releasing hormone to affect prolactin and thyrotropin production in primary rat pituitary cell cultures.DANNIES P, MARKELL M. Differential ability of thyrotropin-releasing hormone and N3im-methyl-thyrotropin-releasing hormone to affect prolactin and thyrotropin production in primary rat pituitary cell cultures. Endocrinology 1980, 106: 107-12. PMID: 6765928, DOI: 10.1210/endo-106-1-107.
- Methods in enzymology Vol. LVIII: Cell Culture. Edited by William B. Jakoby and Ira H. Pastan, Academic Press, New York, 1979, 672 pp. $47.00Dannies P. Methods in enzymology Vol. LVIII: Cell Culture. Edited by William B. Jakoby and Ira H. Pastan, Academic Press, New York, 1979, 672 pp. $47.00. Analytical Biochemistry 1980, 101: 514. DOI: 10.1016/0003-2697(80)90224-9.
- Hormonal control of tyrosine aminotransferase, prolactin, and growth hormone induction in somatic cell hybridsThompson E, Dannies P, Buckler C, Tashjian A. Hormonal control of tyrosine aminotransferase, prolactin, and growth hormone induction in somatic cell hybrids. The Journal Of Steroid Biochemistry And Molecular Biology 1980, 12: 193-210. PMID: 6106733, DOI: 10.1016/0022-4731(80)90269-1.
- Antipsychotic drugs inhibit prolactin release from rat anterior pituitary cells in culture by a mechanism not involving the dopamine receptor.WEST B, DANNIES P. Antipsychotic drugs inhibit prolactin release from rat anterior pituitary cells in culture by a mechanism not involving the dopamine receptor. Endocrinology 1979, 104: 877-80. PMID: 436760, DOI: 10.1210/endo-104-4-877.
- ProlactinDannies P. Prolactin. Trends In Pharmacological Sciences 1979, 1: 206-208. DOI: 10.1016/0165-6147(79)90072-5.
- Comparative Endocrinology of ProlactinDannies P. Comparative Endocrinology of Prolactin. The Yale Journal Of Biology And Medicine 1978, 51: 675-676. PMCID: PMC2595597.
- Anti-estrogenic compounds increase prolactin and growth hormone synthesis in clonal strains of rat pituitary cells.DANNIES P, YEN P, TASHJIAN A. Anti-estrogenic compounds increase prolactin and growth hormone synthesis in clonal strains of rat pituitary cells. Endocrinology 1977, 101: 1151-6. PMID: 908269, DOI: 10.1210/endo-101-4-1151.
- Release and synthesis of prolactin by rat pituitary cell strains are regulated independently by thyrotropin-releasing hormoneDANNIES P, TASJIAN A. Release and synthesis of prolactin by rat pituitary cell strains are regulated independently by thyrotropin-releasing hormone. Nature 1976, 261: 707-710. PMID: 180421, DOI: 10.1038/261707a0.
- Thyrotropin-releasing hormone inreases prolactin mRNA activity in the cytoplasm of GH-cells as measured by translation in a wheat germ cell-free systemDannies P, Tashijian A. Thyrotropin-releasing hormone inreases prolactin mRNA activity in the cytoplasm of GH-cells as measured by translation in a wheat germ cell-free system. Biochemical And Biophysical Research Communications 1976, 70: 1180-1189. PMID: 821486, DOI: 10.1016/0006-291x(76)91027-5.
- A possible role of cyclic AMP in mediating the effects of thyrotropin-releasing hormone on prolactin release and on prolactin and growth hormone synthesis in pituitary cells in culture.DANNIES P, GAUTVIK K, TASHJIAN A. A possible role of cyclic AMP in mediating the effects of thyrotropin-releasing hormone on prolactin release and on prolactin and growth hormone synthesis in pituitary cells in culture. Endocrinology 1976, 98: 1147-59. PMID: 177274, DOI: 10.1210/endo-98-5-1147.
- Pyroglutamyl-histidyl-prolineamide (TRH). A neurohormone which affects the release and synthesis of prolactin and thyrotropin.Dannies P, Tashjian A. Pyroglutamyl-histidyl-prolineamide (TRH). A neurohormone which affects the release and synthesis of prolactin and thyrotropin. Israel Medical Association Journal 1974, 10: 1294-304. PMID: 4215776.
- Effects of Thyrotropin-releasing Hormone and Hydrocortisone on Synthesis and Degradation of Prolactin in a Rat Pituitary Cell StrainDannies P, Tashjian A. Effects of Thyrotropin-releasing Hormone and Hydrocortisone on Synthesis and Degradation of Prolactin in a Rat Pituitary Cell Strain. Journal Of Biological Chemistry 1973, 248: 6174-6179. PMID: 4199262, DOI: 10.1016/s0021-9258(19)43524-2.
- CHAPTER 1 Growth Hormone and Prolactin from Rat Pituitary Tumor Cells11Preparation of this review and the results of original experiments described were supported in part by a research grant from the USPHS (AM 11011). P. S. Dannies is a Postdoctoral Research Fellow of The Arthritis Foundation.Dannies P, Tashjian A. CHAPTER 1 Growth Hormone and Prolactin from Rat Pituitary Tumor Cells11Preparation of this review and the results of original experiments described were supported in part by a research grant from the USPHS (AM 11011). P. S. Dannies is a Postdoctoral Research Fellow of The Arthritis Foundation. 1973, 561-569. DOI: 10.1016/b978-0-12-427150-0.50128-5.
- Structural Properties of Bovine Brain S-100 ProteinDannies P, Levine L. Structural Properties of Bovine Brain S-100 Protein. Journal Of Biological Chemistry 1971, 246: 6276-6283. PMID: 5001787, DOI: 10.1016/s0021-9258(18)61785-5.
- The Role of Sulfhydryl Groups in Serological Properties of Bovine Brain S-100 ProteinDannies P, Levine L. The Role of Sulfhydryl Groups in Serological Properties of Bovine Brain S-100 Protein. Journal Of Biological Chemistry 1971, 246: 6284-6287. PMID: 5001788, DOI: 10.1016/s0021-9258(18)61786-7.
- Investigations of inter- and intramolecular interations in flavin--adenine dinucleotide by proton magnetic resonance.Sarma R, Dannies P, Kaplan N. Investigations of inter- and intramolecular interations in flavin--adenine dinucleotide by proton magnetic resonance. Biochemistry 1968, 7: 4359-67. PMID: 4302624, DOI: 10.1021/bi00852a031.