Micha Sam Brickman Raredon, MD, PhD
Postdoctoral Fellow
Research & Publications
Biography
News
Research Summary
Computational engineering of complex tissues
Extensive Research Description
Dr. Raredon's research group focuses on the mechanisms governing the behavior of complex multicellular systems and in leveraging these principles to engineer living tissues for regenerative medicine. Our work combines systems biology, stem-cell engineering, materials science, surgical fabrication, and biological engineering. The team is particularly invested in engineering, or controlling the self-organization of, cross-length scale perfusable vascular networks and tissue architecture, and in devising principles of microvascular construction and control that can be applied across organ types. We are a clinically-integrated laboratory with two broadly interconnected goals: the modeling of network-level mechanisms of tissue morphogenesis and phenotype regulation, and the application of those models to the design and fabrication of patient-specific tissues for therapeutic use.
Coauthors
Research Image
Bronchioalveolar junction
Immunostaining of cellular communities at the bronchioalveolar junction in lung tissue
Selected Publications
- Spatial Transcriptomic Analysis Allow the Identification of Abnormal Airway Niches in the Fibrotic LungJustet A, Adams T, Balayev A, Baernthaler T, Poli F, Cala Garcia J, Mcdonough J, Flint J, Schupp J, Zhao A, Yunqing L, Ningshan L, Raredon M, Lepe A, Wang A, Abu Hussein N, Yan X, Rosas I, Kaminski N. Spatial Transcriptomic Analysis Allow the Identification of Abnormal Airway Niches in the Fibrotic Lung. 2023, a1033-a1033. DOI: 10.1164/ajrccm-conference.2023.207.1_meetingabstracts.a1033.
- High-dimensional Analysis of Cell-cell Signaling in Pulmonary Biology and Disease Using NichesRaredon M, Yang J, Kothapalli N, Greaney A, Justet A, Adams T, Zhao A, Kim H, Manning E, Medzhitov R, Niklason L, Kaminski N, Kluger Y. High-dimensional Analysis of Cell-cell Signaling in Pulmonary Biology and Disease Using Niches. 2023, a4697-a4697. DOI: 10.1164/ajrccm-conference.2023.207.1_meetingabstracts.a4697.
- Rational engineering of lung alveolar epitheliumLeiby K, Yuan Y, Ng R, Raredon M, Adams T, Baevova P, Greaney A, Hirschi K, Campbell S, Kaminski N, Herzog E, Niklason L. Rational engineering of lung alveolar epithelium. Npj Regenerative Medicine 2023, 8: 22. PMID: 37117221, PMCID: PMC10147714, DOI: 10.1038/s41536-023-00295-2.
- SARS-CoV-2 leverages airway epithelial protective mechanism for viral infectionGreaney A, Raredon M, Kochugaeva M, Niklason L, Levchenko A. SARS-CoV-2 leverages airway epithelial protective mechanism for viral infection. IScience 2023, 26: 106175. PMID: 36788793, PMCID: PMC9912025, DOI: 10.1016/j.isci.2023.106175.
- Comprehensive visualization of cell–cell interactions in single-cell and spatial transcriptomics with NICHESRaredon M, Yang J, Kothapalli N, Lewis W, Kaminski N, Niklason L, Kluger Y. Comprehensive visualization of cell–cell interactions in single-cell and spatial transcriptomics with NICHES. Bioinformatics 2022, 39: btac775. PMID: 36458905, PMCID: PMC9825783, DOI: 10.1093/bioinformatics/btac775.
- Fibroblast inflammatory priming determines regenerative versus fibrotic skin repair in reindeerSinha S, Sparks H, Labit E, Robbins H, Gowing K, Jaffer A, Kutluberk E, Arora R, Raredon M, Cao L, Swanson S, Jiang P, Hee O, Pope H, Workentine M, Todkar K, Sharma N, Bharadia S, Chockalingam K, de Almeida L, Adam M, Niklason L, Potter S, Seifert A, Dufour A, Gabriel V, Rosin N, Stewart R, Muench G, McCorkell R, Matyas J, Biernaskie J. Fibroblast inflammatory priming determines regenerative versus fibrotic skin repair in reindeer. Cell 2022, 185: 4717-4736.e25. PMID: 36493752, PMCID: PMC9888357, DOI: 10.1016/j.cell.2022.11.004.
- Soluble Signals to Improve Endothelial Integrity in the LungYuan Y, Raredon M, Yuan Q, Obata T, Qian H, Wu D, Kaminski N, Niklason L. Soluble Signals to Improve Endothelial Integrity in the Lung. 2022, a5749-a5749. DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a5749.
- Aberrant Mesenchymal Signaling in the COPD LungBrewster R, Neeharika N, Mcdonough J, Adams T, Schupp J, Niklason L, Raredon M, Kaminski N, Rosas I, Sauler M. Aberrant Mesenchymal Signaling in the COPD Lung. 2022, a4655-a4655. DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a4655.
- Computation and visualization of cell–cell signaling topologies in single-cell systems data using ConnectomeRaredon MSB, Yang J, Garritano J, Wang M, Kushnir D, Schupp JC, Adams TS, Greaney AM, Leiby KL, Kaminski N, Kluger Y, Levchenko A, Niklason LE. Computation and visualization of cell–cell signaling topologies in single-cell systems data using Connectome. Scientific Reports 2022, 12: 4187. PMID: 35264704, PMCID: PMC8906120, DOI: 10.1038/s41598-022-07959-x.
- Characterization of the COPD alveolar niche using single-cell RNA sequencingSauler M, McDonough JE, Adams TS, Kothapalli N, Barnthaler T, Werder RB, Schupp JC, Nouws J, Robertson MJ, Coarfa C, Yang T, Chioccioli M, Omote N, Cosme C, Poli S, Ayaub EA, Chu SG, Jensen KH, Gomez JL, Britto CJ, Raredon MSB, Niklason LE, Wilson AA, Timshel PN, Kaminski N, Rosas IO. Characterization of the COPD alveolar niche using single-cell RNA sequencing. Nature Communications 2022, 13: 494. PMID: 35078977, PMCID: PMC8789871, DOI: 10.1038/s41467-022-28062-9.
- Single-cell multi-omics reveals dyssynchrony of the innate and adaptive immune system in progressive COVID-19Unterman A, Sumida TS, Nouri N, Yan X, Zhao AY, Gasque V, Schupp JC, Asashima H, Liu Y, Cosme C, Deng W, Chen M, Raredon MSB, Hoehn KB, Wang G, Wang Z, DeIuliis G, Ravindra NG, Li N, Castaldi C, Wong P, Fournier J, Bermejo S, Sharma L, Casanovas-Massana A, Vogels CBF, Wyllie AL, Grubaugh ND, Melillo A, Meng H, Stein Y, Minasyan M, Mohanty S, Ruff WE, Cohen I, Raddassi K, Niklason L, Ko A, Montgomery R, Farhadian S, Iwasaki A, Shaw A, van Dijk D, Zhao H, Kleinstein S, Hafler D, Kaminski N, Dela Cruz C. Single-cell multi-omics reveals dyssynchrony of the innate and adaptive immune system in progressive COVID-19. Nature Communications 2022, 13: 440. PMID: 35064122, PMCID: PMC8782894, DOI: 10.1038/s41467-021-27716-4.
- Pressure-Regulated Ventilator Splitting for Disaster Relief: Design, Testing, and Clinical ExperienceRaredon MSB, Fisher C, Heerdt PM, Schonberger RB, Nargi A, Nivison S, Fajardo E, Deshpande R, Akhtar S, Greaney AM, Belter J, Raredon T, Zinter J, McKee A, Michalski M, Baevova P, Niklason LE. Pressure-Regulated Ventilator Splitting for Disaster Relief: Design, Testing, and Clinical Experience. Anesthesia & Analgesia 2021, 134: 1094-1105. PMID: 34928890, DOI: 10.1213/ane.0000000000005825.
- A Pulmonary Vascular Model From Endothelialized Whole Organ ScaffoldsYuan Y, Leiby KL, Greaney AM, Raredon MSB, Qian H, Schupp JC, Engler AJ, Baevova P, Adams TS, Kural MH, Wang J, Obata T, Yoder MC, Kaminski N, Niklason LE. A Pulmonary Vascular Model From Endothelialized Whole Organ Scaffolds. Frontiers In Bioengineering And Biotechnology 2021, 9: 760309. PMID: 34869270, PMCID: PMC8640093, DOI: 10.3389/fbioe.2021.760309.
- Microvascular fluid flow in ex vivo and engineered lungsRaredon MSB, Engler AJ, Yuan Y, Greaney AM, Niklason LE. Microvascular fluid flow in ex vivo and engineered lungs. Journal Of Applied Physiology 2021, 131: 1444-1459. PMID: 34554016, PMCID: PMC8616606, DOI: 10.1152/japplphysiol.00286.2020.
- High resolution stereolithography fabrication of perfusable scaffolds to enable long-term meso-scale hepatic culture for disease modelingSphabmixay P, Raredon M, Wang A, Lee H, Hammond P, Fang N, Griffith L. High resolution stereolithography fabrication of perfusable scaffolds to enable long-term meso-scale hepatic culture for disease modeling. Biofabrication 2021, 13: 045024. PMID: 34479229, DOI: 10.1088/1758-5090/ac23aa.
- Mechanisms of Hypoxia-Induced Pulmonary Arterial Stiffening in Mice Revealed by a Functional Genetics Assay of Structural, Functional, and Transcriptomic DataManning EP, Ramachandra AB, Schupp JC, Cavinato C, Raredon MSB, Bärnthaler T, Cosme C, Singh I, Tellides G, Kaminski N, Humphrey JD. Mechanisms of Hypoxia-Induced Pulmonary Arterial Stiffening in Mice Revealed by a Functional Genetics Assay of Structural, Functional, and Transcriptomic Data. Frontiers In Physiology 2021, 12: 726253. PMID: 34594238, PMCID: PMC8478173, DOI: 10.3389/fphys.2021.726253.
- Aberrant endothelial CXCL signaling in COPDKothapalli N, Mcdonough J, Raredon M, Adams T, Schupp J, Niklason L, Kaminski N, Rosas I, Sauler M. Aberrant endothelial CXCL signaling in COPD. 2021, pa3623. DOI: 10.1183/13993003.congress-2021.pa3623.
- Integrated Single-Cell Atlas of Endothelial Cells of the Human LungSchupp JC, Adams TS, Cosme C, Raredon MSB, Yuan Y, Omote N, Poli S, Chioccioli M, Rose KA, Manning EP, Sauler M, DeIuliis G, Ahangari F, Neumark N, Habermann AC, Gutierrez AJ, Bui LT, Lafyatis R, Pierce RW, Meyer KB, Nawijn MC, Teichmann SA, Banovich NE, Kropski JA, Niklason LE, Pe’er D, Yan X, Homer RJ, Rosas IO, Kaminski N. Integrated Single-Cell Atlas of Endothelial Cells of the Human Lung. Circulation 2021, 144: 286-302. PMID: 34030460, PMCID: PMC8300155, DOI: 10.1161/circulationaha.120.052318.
- Abstract 14125: An in vitro Pulmonary Vascular Platform From Endothelialized Whole Lung ScaffoldsYuan Y, Leiby K, Greaney A, Raredon M, Qian H, Baevova P, Admas T, Engler A, Kural M, Schupp J, Hong Y, Kaminski N, Niklason L. Abstract 14125: An in vitro Pulmonary Vascular Platform From Endothelialized Whole Lung Scaffolds. Circulation 2020, 142 DOI: 10.1161/circ.142.suppl_3.14125.
- Bioengineering the Blood‐gas BarrierLeiby KL, Raredon MSB, Niklason LE. Bioengineering the Blood‐gas Barrier. 2020, 10: 415-452. PMID: 32163210, PMCID: PMC7366783, DOI: 10.1002/cphy.c190026.
- Platform Effects on Regeneration by Pulmonary Basal Cells as Evaluated by Single-Cell RNA SequencingGreaney AM, Adams TS, Raredon M, Gubbins E, Schupp JC, Engler AJ, Ghaedi M, Yuan Y, Kaminski N, Niklason LE. Platform Effects on Regeneration by Pulmonary Basal Cells as Evaluated by Single-Cell RNA Sequencing. Cell Reports 2020, 30: 4250-4265.e6. PMID: 32209482, PMCID: PMC7175071, DOI: 10.1016/j.celrep.2020.03.004.
- Chapter 68 Lung tissue engineeringRaredon M, Yuan Y, Niklason L. Chapter 68 Lung tissue engineering. 2020, 1273-1285. DOI: 10.1016/b978-0-12-818422-6.00070-8.
- Single-cell connectomic analysis of adult mammalian lungsRaredon MSB, Adams TS, Suhail Y, Schupp JC, Poli S, Neumark N, Leiby KL, Greaney AM, Yuan Y, Horien C, Linderman G, Engler AJ, Boffa DJ, Kluger Y, Rosas IO, Levchenko A, Kaminski N, Niklason LE. Single-cell connectomic analysis of adult mammalian lungs. Science Advances 2019, 5: eaaw3851. PMID: 31840053, PMCID: PMC6892628, DOI: 10.1126/sciadv.aaw3851.
- Engineering of human brain organoids with a functional vascular-like systemCakir B, Xiang Y, Tanaka Y, Kural MH, Parent M, Kang YJ, Chapeton K, Patterson B, Yuan Y, He CS, Raredon MSB, Dengelegi J, Kim KY, Sun P, Zhong M, Lee S, Patra P, Hyder F, Niklason LE, Lee SH, Yoon YS, Park IH. Engineering of human brain organoids with a functional vascular-like system. Nature Methods 2019, 16: 1169-1175. PMID: 31591580, PMCID: PMC6918722, DOI: 10.1038/s41592-019-0586-5.
- Non-invasive and real-time measurement of microvascular barrier in intact lungsEngler AJ, Raredon MSB, Le AV, Yuan Y, Oczkowicz YA, Kan EL, Baevova P, Niklason LE. Non-invasive and real-time measurement of microvascular barrier in intact lungs. Biomaterials 2019, 217: 119313. PMID: 31280072, PMCID: PMC6863174, DOI: 10.1016/j.biomaterials.2019.119313.
- Epac agonist improves barrier function in iPSC-derived endothelial colony forming cells for whole organ tissue engineeringYuan Y, Engler AJ, Raredon MS, Le A, Baevova P, Yoder MC, Niklason LE. Epac agonist improves barrier function in iPSC-derived endothelial colony forming cells for whole organ tissue engineering. Biomaterials 2019, 200: 25-34. PMID: 30754017, DOI: 10.1016/j.biomaterials.2019.02.005.
- Lung regenerationCalle EA, Leiby KL, Raredon MB, Niklason LE. Lung regeneration. Current Opinion In Anaesthesiology 2017, 30: 23-29. PMID: 27922848, PMCID: PMC5283945, DOI: 10.1097/aco.0000000000000425.
- Biomimetic Culture Reactor for Whole-Lung EngineeringRaredon MS, Rocco KA, Gheorghe CP, Sivarapatna A, Ghaedi M, Balestrini JL, Raredon TL, Calle EA, Niklason LE. Biomimetic Culture Reactor for Whole-Lung Engineering. BioResearch Open Access 2016, 5: 72-83. PMID: 27088061, PMCID: PMC4827315, DOI: 10.1089/biores.2016.0006.
- A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory EpitheliumRaredon MS, Ghaedi M, Calle EA, Niklason LE. A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium. Cell Medicine 2015, 7: 109-121. PMID: 26858899, PMCID: PMC4735574, DOI: 10.3727/215517914x681794.
- Photopatterning of hydrogel scaffolds coupled to filter materials using stereolithography for perfused 3D culture of hepatocytesNeiman JA, Raman R, Chan V, Rhoads MG, Raredon MS, Velazquez JJ, Dyer RL, Bashir R, Hammond PT, Griffith LG. Photopatterning of hydrogel scaffolds coupled to filter materials using stereolithography for perfused 3D culture of hepatocytes. Biotechnology And Bioengineering 2015, 112: 777-787. PMID: 25384798, PMCID: PMC4624302, DOI: 10.1002/bit.25494.
- Bioreactor technologies to support liver function in vitroEbrahimkhani MR, Neiman JA, Raredon MS, Hughes DJ, Griffith LG. Bioreactor technologies to support liver function in vitro. Advanced Drug Delivery Reviews 2014, 69: 132-157. PMID: 24607703, PMCID: PMC4144187, DOI: 10.1016/j.addr.2014.02.011.
- A Call to CraftRaredon MS, Niklason LE. A Call to Craft. Science Translational Medicine 2014, 6: 218fs1. PMID: 24401937, PMCID: PMC4122270, DOI: 10.1126/scitranslmed.3008229.
- Alveolar epithelial differentiation of human induced pluripotent stem cells in a rotating bioreactorGhaedi M, Mendez JJ, Bove PF, Sivarapatna A, Raredon MS, Niklason LE. Alveolar epithelial differentiation of human induced pluripotent stem cells in a rotating bioreactor. Biomaterials 2013, 35: 699-710. PMID: 24144903, PMCID: PMC3897000, DOI: 10.1016/j.biomaterials.2013.10.018.
- Tissue-Engineered Lungs for in Vivo ImplantationPetersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon MB, Gavrilov K, Yi T, Zhuang ZW, Breuer C, Herzog E, Niklason LE. Tissue-Engineered Lungs for in Vivo Implantation. Science 2010, 329: 538-541. PMID: 20576850, PMCID: PMC3640463, DOI: 10.1126/science.1189345.