Michael Stankewich, PhD
Associate Research Scientist in Pathology
Research & Publications
Biography
Locations
Extensive Research Description
A major player of the neuronal cortical cytoskeleton is spectrin, a large and abundant scaffolding protein. The spectrin gene family is comprised of seven genes encoding two alpha and five beta subunits. To add to the complexity, many pre-mRNA splice variants have been identified. The ascribed function for spectrin is stabilizing membranes and organizing protein and lipid micro domains on both the plasma membrane and intracellular organelles. Not only is spectrin important in maintaining membrane micro domains, but also is likely involved in their targeting and transport. Salient characteristics of the spectrin gene family include: 1) a diverse neuronal compartmentalization, 2) Ca2+ /calmodulin mediated regulation, 3) calpain/caspase mediated proteolysis, and 4) tyrosine phosphorylation and ubiquination. My interest is to better elucidate the roles for such sub cellular compartmentalization and posttranslational modifications of the spectrin cytoskeleton and how spectrin affects receptor targeting, trafficking (exocytosis and endocytosis) and stabilization at pre/postsynaptic membranes.
Coauthors
Selected Publications
- Age-dependent ataxia and neurodegeneration caused by an αII spectrin mutation with impaired regulation of its calpain sensitivityMiazek A, Zalas M, Skrzymowska J, Bogin BA, Grzymajło K, Goszczynski TM, Levine ZA, Morrow JS, Stankewich MC. Age-dependent ataxia and neurodegeneration caused by an αII spectrin mutation with impaired regulation of its calpain sensitivity. Scientific Reports 2021, 11: 7312. PMID: 33790315, PMCID: PMC8012654, DOI: 10.1038/s41598-021-86470-1.
- βIII Spectrin Is Necessary for Formation of the Constricted Neck of Dendritic Spines and Regulation of Synaptic Activity in NeuronsEfimova N, Korobova F, Stankewich MC, Moberly AH, Stolz DB, Wang J, Kashina A, Ma M, Svitkina T. βIII Spectrin Is Necessary for Formation of the Constricted Neck of Dendritic Spines and Regulation of Synaptic Activity in Neurons. Journal Of Neuroscience 2017, 37: 6442-6459. PMID: 28576936, PMCID: PMC5511878, DOI: 10.1523/jneurosci.3520-16.2017.
- A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of RanvierHo TS, Zollinger DR, Chang KJ, Xu M, Cooper EC, Stankewich MC, Bennett V, Rasband MN. A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier. Nature Neuroscience 2014, 17: 1664-1672. PMID: 25362473, PMCID: PMC4271271, DOI: 10.1038/nn.3859.
- Intrinsic indicators for specimen degradationLi J, Kil C, Considine K, Smarkucki B, Stankewich MC, Balgley B, Vortmeyer AO. Intrinsic indicators for specimen degradation. Laboratory Investigation 2012, 93: 242-253. PMID: 23212099, DOI: 10.1038/labinvest.2012.164.
- A Distal Axonal Cytoskeleton Forms an Intra-Axonal Boundary that Controls Axon Initial Segment AssemblyGaliano MR, Jha S, Ho TS, Zhang C, Ogawa Y, Chang KJ, Stankewich MC, Mohler PJ, Rasband MN. A Distal Axonal Cytoskeleton Forms an Intra-Axonal Boundary that Controls Axon Initial Segment Assembly. Cell 2012, 149: 1125-1139. PMID: 22632975, PMCID: PMC3361702, DOI: 10.1016/j.cell.2012.03.039.
- Cell organization, growth, and neural and cardiac development require αII-spectrinStankewich MC, Cianci CD, Stabach PR, Ji L, Nath A, Morrow JS. Cell organization, growth, and neural and cardiac development require αII-spectrin. Journal Of Cell Science 2011, 124: 3956-3966. PMID: 22159418, PMCID: PMC3244980, DOI: 10.1242/jcs.080374.
- Schwann cell spectrins modulate peripheral nerve myelinationSusuki K, Raphael AR, Ogawa Y, Stankewich MC, Peles E, Talbot WS, Rasband MN. Schwann cell spectrins modulate peripheral nerve myelination. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 8009-8014. PMID: 21518878, PMCID: PMC3093478, DOI: 10.1073/pnas.1019600108.
- Targeted deletion of βIII spectrin impairs synaptogenesis and generates ataxic and seizure phenotypesStankewich MC, Gwynn B, Ardito T, Ji L, Kim J, Robledo RF, Lux SE, Peters LL, Morrow JS. Targeted deletion of βIII spectrin impairs synaptogenesis and generates ataxic and seizure phenotypes. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 6022-6027. PMID: 20231455, PMCID: PMC2851889, DOI: 10.1073/pnas.1001522107.
- HSP70 binding modulates detachment of Na-K-ATPase following energy deprivation in renal epithelial cellsRiordan M, Sreedharan R, Wang S, Thulin G, Mann A, Stankewich M, Van Why S, Kashgarian M, Siegel NJ. HSP70 binding modulates detachment of Na-K-ATPase following energy deprivation in renal epithelial cells. American Journal Of Physiology. Renal Physiology 2005, 288: f1236-f1242. PMID: 15701813, DOI: 10.1152/ajprenal.00438.2004.
- Fragmentation of the Golgi Apparatus A ROLE FOR βIII SPECTRIN AND SYNTHESIS OF PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE*Siddhanta A, Radulescu A, Stankewich MC, Morrow JS, Shields D. Fragmentation of the Golgi Apparatus A ROLE FOR βIII SPECTRIN AND SYNTHESIS OF PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE*. Journal Of Biological Chemistry 2002, 278: 1957-1965. PMID: 12411436, DOI: 10.1074/jbc.m209137200.
- Regulation of TGF-β ligand and receptor expression in neonatal rat lungs exposed to chronic hypoxiaVicencio AG, Eickelberg O, Stankewich MC, Kashgarian M, Haddad GG. Regulation of TGF-β ligand and receptor expression in neonatal rat lungs exposed to chronic hypoxia. Journal Of Applied Physiology 2002, 93: 1123-1130. PMID: 12183510, DOI: 10.1152/japplphysiol.00031.2002.
- Occludin is a functional component of the tight junctionMcCarthy K, Skare I, Stankewich M, Furuse M, Tsukita S, Rogers R, Lynch R, Schneeberger E. Occludin is a functional component of the tight junction. Journal Of Cell Science 1996, 109: 2287-2298. PMID: 8886979, DOI: 10.1242/jcs.109.9.2287.