Mei-Ling Yang, PhD
Associate Research Scientist in Medicine (Rheumatology)
Research & Publications
Biography
News
Extensive Research Description
Post-translational protein modification influences immunologic responses ranging from intracellular signaling to protein processing and presentation and creates neo-epitopes from self-proteins to break immune tolerance that leads to autoimmune diseases. Systemic erythematosus lupus (SLE) and type 1 diabetes (T1D) are the complex autoimmune diseases involving multiple genetic and environmental factors. My research is to investigate the role of posttranslational protein modifications including isoaspartyl modification, methylation, carbonylation and citrullination on genetic association analysis and biological functions of T cells in the pathogenesis of SLE and T1D. My work provides new insight into the diagnosis of autoimmune diseases where the specific auto-antigen is unknown and provides targets in autoimmune pathways to discover novel therapeutic concepts and biomarkers for inflammatory conditions.
Coauthors
Research Interests
Autoimmune Diseases; Diabetes Mellitus, Type 1; Lupus Erythematosus, Systemic
Selected Publications
- A discovery-based proteomics approach identifies protein disulphide isomerase (PDIA1) as a biomarker of β cell stress in type 1 diabetesSyed F, Singhal D, Raedschelders K, Krishnan P, Bone R, McLaughlin M, Van Eyk J, Mirmira R, Yang M, Mamula M, Wu H, Liu X, Evans-Molina C. A discovery-based proteomics approach identifies protein disulphide isomerase (PDIA1) as a biomarker of β cell stress in type 1 diabetes EBioMedicine 2022, 87: 104379. PMID: 36463755, PMCID: PMC9719098, DOI: 10.1016/j.ebiom.2022.104379.
- Biomarkers of autoimmunity and beta cell metabolism in type 1 diabetesYang M, Kibbey R, Mamula M. Biomarkers of autoimmunity and beta cell metabolism in type 1 diabetes Frontiers In Immunology 2022, 13: 1028130. PMCID: PMC9647083, DOI: 10.3389/fimmu.2022.1028130.
- Biomarkers of autoimmunity and beta cell metabolism in type 1 diabetesYang M, Kibbey R, Mamula M. Biomarkers of autoimmunity and beta cell metabolism in type 1 diabetes Frontiers In Immunology 2022, 13: 1028130. PMID: 36389721, PMCID: PMC9647083, DOI: 10.3389/fimmu.2022.1028130.
- Immune cells and their inflammatory mediators modify beta cells and cause checkpoint inhibitor-induced diabetesPerdigoto AL, Deng S, Du KC, Kuchroo M, Burkhardt DB, Tong A, Israel G, Robert ME, Weisberg SP, Kirkiles-Smith N, Stamatouli AM, Kluger HM, Quandt Z, Young A, Yang ML, Mamula MJ, Pober JS, Anderson MS, Krishnaswamy S, Herold KC. Immune cells and their inflammatory mediators modify beta cells and cause checkpoint inhibitor-induced diabetes JCI Insight 2022, 7: e156330. PMID: 35925682, PMCID: PMC9536276, DOI: 10.1172/jci.insight.156330.
- Carbonyl Posttranslational Modification Associated With Early-Onset Type 1 Diabetes Autoimmunity.Yang ML, Connolly SE, Gee RJ, Lam TT, Kanyo J, Peng J, Guyer P, Syed F, Tse HM, Clarke SG, Clarke CF, James EA, Speake C, Evans-Molina C, Arvan P, Herold KC, Wen L, Mamula MJ. Carbonyl Posttranslational Modification Associated With Early-Onset Type 1 Diabetes Autoimmunity. Diabetes 2022, 71: 1979-1993. PMID: 35730902, PMCID: PMC9450849, DOI: 10.2337/db21-0989.
- Regulation of metabolism by protein modifications in autoimmunityYang M, James E, Overbergh L, Herold K, Mamula M. Regulation of metabolism by protein modifications in autoimmunity The Journal Of Immunology 2022, 208: 108.09-108.09. DOI: 10.4049/jimmunol.208.supp.108.09.
- Citrullination of glucokinase is linked to autoimmune diabetesYang ML, Horstman S, Gee R, Guyer P, Lam TT, Kanyo J, Perdigoto AL, Speake C, Greenbaum CJ, Callebaut A, Overbergh L, Kibbey RG, Herold KC, James EA, Mamula MJ. Citrullination of glucokinase is linked to autoimmune diabetes Nature Communications 2022, 13: 1870. PMID: 35388005, PMCID: PMC8986778, DOI: 10.1038/s41467-022-29512-0.
- Citrullination and PAD Enzyme Biology in Type 1 Diabetes – Regulators of Inflammation, Autoimmunity, and PathologyYang ML, Sodré FMC, Mamula MJ, Overbergh L. Citrullination and PAD Enzyme Biology in Type 1 Diabetes – Regulators of Inflammation, Autoimmunity, and Pathology Frontiers In Immunology 2021, 12: 678953. PMID: 34140951, PMCID: PMC8204103, DOI: 10.3389/fimmu.2021.678953.
- Inflammation-Induced Citrullinated Glucose-Regulated Protein 78 Elicits Immune Responses in Human Type 1 DiabetesBuitinga M, Callebaut A, Sodré F, Crèvecoeur I, Blahnik-Fagan G, Yang ML, Bugliani M, Arribas-Layton D, Marré M, Cook DP, Waelkens E, Mallone R, Piganelli JD, Marchetti P, Mamula MJ, Derua R, James EA, Mathieu C, Overbergh L. Inflammation-Induced Citrullinated Glucose-Regulated Protein 78 Elicits Immune Responses in Human Type 1 Diabetes Diabetes 2018, 67: 2337-2348. PMID: 30348823, PMCID: PMC6973547, DOI: 10.2337/db18-0295.
- Oxidative Modifications in Tissue Pathology and Autoimmune DiseaseYang ML, Doyle H, Clarke SG, Herold K, Mamula M. Oxidative Modifications in Tissue Pathology and Autoimmune Disease Antioxidants And Redox Signaling 2017, 29: 1415-1431. PMID: 29088923, PMCID: PMC6166690, DOI: 10.1089/ars.2017.7382.
- The role for neutrophil extracellular traps in cystic fibrosis autoimmunitySkopelja S, Hamilton BJ, Jones JD, Yang ML, Mamula M, Ashare A, Gifford AH, Rigby WF. The role for neutrophil extracellular traps in cystic fibrosis autoimmunity JCI Insight 2016, 1: e88912. PMID: 27777975, PMCID: PMC5070963, DOI: 10.1172/jci.insight.88912.
- Isoaspartyl modification of ZAP70 signaling molecule alters T cell responses (555.9)Yang M, Clarke S, Mamula M. Isoaspartyl modification of ZAP70 signaling molecule alters T cell responses (555.9) The FASEB Journal 2014, 28 DOI: 10.1096/fasebj.28.1_supplement.555.9.
- Autoantigens: Novel forms and presentation to the immune systemDoyle HA, Yang ML, Raycroft MT, Gee RJ, Mamula MJ. Autoantigens: Novel forms and presentation to the immune system Autoimmunity 2013, 47: 220-233. PMID: 24191689, DOI: 10.3109/08916934.2013.850495.
- Lupus autoimmunity altered by cellular methylation metabolismYang ML, Gee AJ, Gee RJ, Zurita-Lopez CI, Khare S, Clarke SG, Mamula MJ. Lupus autoimmunity altered by cellular methylation metabolism Autoimmunity 2012, 46: 21-31. PMID: 23039363, PMCID: PMC3543504, DOI: 10.3109/08916934.2012.732133.
- S-Adenosylmethionine-dependent methylation modulates T cell immune response and lupus autoimmunity (116.13)Yang M, Gee A, Gee R, Mamula M. S-Adenosylmethionine-dependent methylation modulates T cell immune response and lupus autoimmunity (116.13) The Journal Of Immunology 2012, 188: 116.13-116.13. DOI: 10.4049/jimmunol.188.supp.116.13.
- Isoaspartyl Posttranslational Modification In Drug‐Induced LupusYang M, Mamula M. Isoaspartyl Posttranslational Modification In Drug‐Induced Lupus The FASEB Journal 2008, 22: 668.20-668.20. DOI: 10.1096/fasebj.22.1_supplement.668.20.
- Intracellular protein modification associated with altered T cell functions in autoimmunityYang M, Doyle H, Gee R, Lowenson J, Clarke S, Lawson B, Aswad D, Mamula M. Intracellular protein modification associated with altered T cell functions in autoimmunity The Journal Of Immunology 2006, 177: 8878-8878. DOI: 10.4049/jimmunol.177.12.8878.
- Intracellular Protein Modification Associated with Altered T Cell Functions in AutoimmunityYang ML, Doyle HA, Gee RJ, Lowenson JD, Clarke S, Lawson BR, Aswad DW, Mamula MJ. Intracellular Protein Modification Associated with Altered T Cell Functions in Autoimmunity The Journal Of Immunology 2006, 177: 4541-4549. PMID: 16982891, DOI: 10.4049/jimmunol.177.7.4541.
- Increase in intracellular protein modification associated with altered lymphocyte functions in autoimmunityYang M, Doyle H, Gee R, Aswad D, Mamula M. Increase in intracellular protein modification associated with altered lymphocyte functions in autoimmunity The FASEB Journal 2006, 20: a964-a964. DOI: 10.1096/fasebj.20.5.a964-a.