2000
Hsl1p, a Swe1p Inhibitor, Is Degraded via the Anaphase-Promoting Complex
Burton J, Solomon M. Hsl1p, a Swe1p Inhibitor, Is Degraded via the Anaphase-Promoting Complex. Molecular And Cellular Biology 2000, 20: 4614-4625. PMID: 10848588, PMCID: PMC85864, DOI: 10.1128/mcb.20.13.4614-4625.2000.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsAnaphase-Promoting Complex-CyclosomeApc8 Subunit, Anaphase-Promoting Complex-CyclosomeBase SequenceCdc20 ProteinsCdh1 ProteinsCell CycleCell Cycle ProteinsCyclin-Dependent KinasesFungal ProteinsGenes, DominantLigasesMolecular Sequence DataMutationPrecipitin TestsProtein KinasesProtein Serine-Threonine KinasesProtein-Tyrosine KinasesSaccharomyces cerevisiae ProteinsTwo-Hybrid System TechniquesUbiquitin-Protein Ligase ComplexesUbiquitin-Protein LigasesYeastsConceptsAnaphase-promoting complexDestruction box motifCell cycle eventsProtein kinaseBox motifCycle eventsCyclin-dependent kinase Cdc28pCritical cell cycle regulatorsAPC-dependent mannerCell cycle regulatorsSwe1p degradationMorphogenesis checkpointAPC substratesHsl1pLate mitosisProper progressionProtein substratesUbiquitin ligaseCoimmunoprecipitation studiesSequence homologyCycle regulatorsUbiquitinationSubsequent degradationKinaseCdc20p
1999
The CDK-activating Kinase (Cak1p) from Budding Yeast Has an Unusual ATP-binding Pocket*
Enke D, Kaldis P, Holmes J, Solomon M. The CDK-activating Kinase (Cak1p) from Budding Yeast Has an Unusual ATP-binding Pocket*. Journal Of Biological Chemistry 1999, 274: 1949-1956. PMID: 9890950, DOI: 10.1074/jbc.274.4.1949.Peer-Reviewed Original ResearchConceptsProtein kinaseInvariant lysineMajor cyclin-dependent kinaseLoop regionEssential protein kinaseMost protein kinasesAmino acidsATP-binding pocketCyclin-dependent kinasesBudding YeastCak1pMutagenic analysisATP phosphatesSequence differencesLoop motifKinaseCovalent modificationCore sequenceATP analogYeastCatalytic rateInhibitory drugsLysineMutationsATP
1995
KIN28 Encodes a C-Terminal Domain Kinase That Controls mRNA Transcription in Saccharomyces cerevisiae but Lacks Cyclin-Dependent Kinase-Activating Kinase (CAK) Activity
Cismowski M, Laff G, Solomon M, Reed S. KIN28 Encodes a C-Terminal Domain Kinase That Controls mRNA Transcription in Saccharomyces cerevisiae but Lacks Cyclin-Dependent Kinase-Activating Kinase (CAK) Activity. Molecular And Cellular Biology 1995, 15: 2983-2992. PMID: 7760796, PMCID: PMC230529, DOI: 10.1128/mcb.15.6.2983.Peer-Reviewed Original ResearchConceptsC-terminal domainKinase activityCTD kinaseMRNA transcriptionCritical threonine residueCyclin-dependent kinase familyCTD kinase activityRNA polymerase IICell cycle CDKsExtensive sequence identityCyclin-dependent kinasesThermosensitive alleleCTD phosphorylationPolymerase IIThreonine residuesKinase familyPositive regulatorKin28Phosphorylation stateS. cerevisiaeSequence identityCell cycleTranscriptionHuman cellsCDK
1993
Phosphorylation independent activation of human cyclin-dependent kinase 2 by cyclin A in vitro.
Connell-Crowley L, Solomon M, Wei N, Harper J. Phosphorylation independent activation of human cyclin-dependent kinase 2 by cyclin A in vitro. Molecular Biology Of The Cell 1993, 4: 79-92. PMID: 8443411, PMCID: PMC300902, DOI: 10.1091/mbc.4.1.79.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCDC2-CDC28 KinasesCyclin-Dependent Kinase 2Cyclin-Dependent KinasesCyclinsDNAEnzyme ActivationHumansIn Vitro TechniquesMolecular Sequence DataMutagenesis, Site-DirectedPhosphorylationProtein KinasesProtein Serine-Threonine KinasesSaccharomyces cerevisiaeXenopusXenopus ProteinsConceptsCDK2/cyclinCyclin-dependent kinase 2Cyclin AKinase 2Cyclin BPhosphorylation-independent activationSerine-threonine proteinCdc2/cyclin BCDK2/cyclin AHuman cyclin-dependent kinase 2G1/S transitionHistone H1 kinaseCyclin B complexCell cycle progressionSpecific activityCyclin bindingRecombinant CDK2Mammalian cellsH1 kinaseBacterial expressionT160 phosphorylationCycle progressionXenopus eggsCell extractsCyclin