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Lokesh Kumar Sharma, PhD

Assistant Professor

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Lokesh Kumar Sharma, PhD

Research Summary

My research focus is to understand the host-pathogen interactions during pulmonary infections with bacterial and viral pathogens.

Coauthors

Selected Publications

  • Cutting Edge: Severe SARS-CoV-2 Infection in Humans Is Defined by a Shift in the Serum Lipidome, Resulting in Dysregulation of Eicosanoid Immune Mediators.Schwarz B, Sharma L, Roberts L, Peng X, Bermejo S, Leighton I, Casanovas-Massana A, Minasyan M, Farhadian S, Ko AI, Dela Cruz CS, Bosio CM. Cutting Edge: Severe SARS-CoV-2 Infection in Humans Is Defined by a Shift in the Serum Lipidome, Resulting in Dysregulation of Eicosanoid Immune Mediators. Journal Of Immunology (Baltimore, Md. : 1950) 2021, 206: 329-334. PMID: 33277388, PMCID: PMC7962598, DOI: 10.4049/jimmunol.2001025.
  • JAK-inhibitor and type I interferon ability to produce favorable clinical outcomes in COVID-19 patients: a systematic review and meta-analysis.Walz L, Cohen AJ, Rebaza AP, Vanchieri J, Slade MD, Dela Cruz CS, Sharma L. JAK-inhibitor and type I interferon ability to produce favorable clinical outcomes in COVID-19 patients: a systematic review and meta-analysis. BMC Infectious Diseases 2021, 21: 47. PMID: 33430799, PMCID: PMC7797881, DOI: 10.1186/s12879-020-05730-z.
  • Disinfection of Pseudomonas aeruginosa from N95 respirators with ozone: a pilot study.Manning EP, Stephens MD, Dufresne S, Silver B, Gerbarg P, Gerbarg Z, Dela Cruz CS, Sharma L. Disinfection of Pseudomonas aeruginosa from N95 respirators with ozone: a pilot study. BMJ Open Respiratory Research 2021, 8 PMID: 33431507, PMCID: PMC7802653, DOI: 10.1136/bmjresp-2020-000781.
  • PINK1 Inhibits Multimeric Aggregation and Signaling of MAVS and MAVS-Dependent Lung Pathology.Kim SH, Shin HJ, Yoon CM, Lee SW, Sharma L, Dela Cruz CS, Kang MJ. PINK1 Inhibits Multimeric Aggregation and Signaling of MAVS and MAVS-Dependent Lung Pathology. American Journal Of Respiratory Cell And Molecular Biology 2021, 64: 592-603. PMID: 33577398, PMCID: PMC8086043, DOI: 10.1165/rcmb.2020-0490OC.
  • RIPK3 Activates MLKL-mediated Necroptosis and Inflammasome Signaling during Streptococcus Infection.Huang HR, Cho SJ, Harris RM, Yang J, Bermejo S, Sharma L, Dela Cruz CS, Xu JF, Stout-Delgado HW. RIPK3 Activates MLKL-mediated Necroptosis and Inflammasome Signaling during Streptococcus Infection. American Journal Of Respiratory Cell And Molecular Biology 2021, 64: 579-591. PMID: 33625952, PMCID: PMC8086037, DOI: 10.1165/rcmb.2020-0312OC.
  • Challenges in understanding lung microbiome: It is NOT like the gut microbiome.Chang, Dela Cruz CS, Sharma L. Challenges in understanding lung microbiome: It is NOT like the gut microbiome. Respirology (Carlton, Vic.) 2020, 25: 244-245. PMID: 31868282, DOI: 10.1111/resp.13759.
  • Epidemiologic and Clinical Characteristics of Novel Coronavirus Infections Involving 13 Patients Outside Wuhan, China.Chang, Lin M, Wei L, Xie L, Zhu G, Dela Cruz CS, Sharma L. Epidemiologic and Clinical Characteristics of Novel Coronavirus Infections Involving 13 Patients Outside Wuhan, China. JAMA 2020, 323: 1092-1093. PMID: 32031568, PMCID: PMC7042871, DOI: 10.1001/jama.2020.1623.
  • Protecting health-care workers from subclinical coronavirus infection.Chang, Xu H, Rebaza A, Sharma L, Dela Cruz CS. Protecting health-care workers from subclinical coronavirus infection. The Lancet. Respiratory Medicine 2020, 8: e13. PMID: 32061333, PMCID: PMC7128440, DOI: 10.1016/S2213-2600(20)30066-7.
  • Mechanisms of Epithelial Immunity Evasion by Respiratory Bacterial Pathogens.Sharma L, Feng J, Britto CJ, Dela Cruz CS. Mechanisms of Epithelial Immunity Evasion by Respiratory Bacterial Pathogens. Frontiers In Immunology 2020, 11: 91. PMID: 32117248, PMCID: PMC7027138, DOI: 10.3389/fimmu.2020.00091.
  • Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19).Guo L, Ren L, Yang S, Xiao M, Chang, Yang F, Dela Cruz CS, Wang Y, Wu C, Xiao Y, Zhang L, Han L, Dang S, Xu Y, Yang Q, Xu S, Zhu H, Xu Y, Jin Q, Sharma L, Wang L, Wang J. Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19). Clinical Infectious Diseases : An Official Publication Of The Infectious Diseases Society Of America 2020, 71: 778-785. PMID: 32198501, PMCID: PMC7184472, DOI: 10.1093/cid/ciaa310.
  • Time Kinetics of Viral Clearance and Resolution of Symptoms in Novel Coronavirus Infection.Chang, Mo G, Yuan X, Tao Y, Peng X, Wang FS, Xie L, Sharma L, Dela Cruz CS, Qin E. Time Kinetics of Viral Clearance and Resolution of Symptoms in Novel Coronavirus Infection. American Journal Of Respiratory And Critical Care Medicine 2020, 201: 1150-1152. PMID: 32200654, PMCID: PMC7193851, DOI: 10.1164/rccm.202003-0524LE.
  • Harnessing Murine Microbiome Models to Study Human Lung Microbiome.Chang, Sharma L, Dela Cruz CS. Harnessing Murine Microbiome Models to Study Human Lung Microbiome. Chest 2020, 157: 776-778. PMID: 32252931, PMCID: PMC7926993, DOI: 10.1016/j.chest.2019.12.011.
  • Chitotriosidase: a marker and modulator of lung disease.Chang, Sharma L, Dela Cruz CS. Chitotriosidase: a marker and modulator of lung disease. European Respiratory Review : An Official Journal Of The European Respiratory Society 2020, 29 PMID: 32350087, DOI: 10.1183/16000617.0143-2019.
  • Reply to Suri et al.: COVID-19 Real-Time RT-PCR: Does Positivity on Follow-up RT-PCR Always Imply Infectivity?Chang, Mo G, Yuan X, Tao Y, Peng X, Wang F, Xie L, Sharma L, Dela Cruz CS, Qin E. Reply to Suri et al.: COVID-19 Real-Time RT-PCR: Does Positivity on Follow-up RT-PCR Always Imply Infectivity? American Journal Of Respiratory And Critical Care Medicine 2020, 202: 148. PMID: 32401535, PMCID: PMC7328309, DOI: 10.1164/rccm.202004-1458LE.
  • Persistent Viral Presence Determines the Clinical Course of the Disease in COVID-19.Chang, Zhao P, Zhang D, Dong JH, Xu Z, Yang G, Li BY, Liu HX, Li BA, Qin CF, Peng XH, Wang FS, Xie LX, Chen Z, Dela Cruz CS, Sharma L, Qin EQ. Persistent Viral Presence Determines the Clinical Course of the Disease in COVID-19. The Journal Of Allergy And Clinical Immunology. In Practice 2020, 8: 2585-2591.e1. PMID: 32574840, PMCID: PMC7305869, DOI: 10.1016/j.jaip.2020.06.015.
  • Severe SARS-CoV-2 infection in humans is defined by a shift in the serum lipidome resulting in dysregulation of eicosanoid immune mediators.Schwarz B, Sharma L, Roberts L, Peng X, Bermejo S, Leighton I, Massana AC, Farhadian S, Ko A, DelaCruz C, Bosio CM. Severe SARS-CoV-2 infection in humans is defined by a shift in the serum lipidome resulting in dysregulation of eicosanoid immune mediators. MedRxiv : The Preprint Server For Health Sciences 2020 PMID: 32676616, PMCID: PMC7359541, DOI: 10.1101/2020.07.09.20149849.
  • Severe SARS-CoV-2 infection in humans is defined by a shift in the serum lipidome resulting in dysregulation of eicosanoid immune mediators.Schwarz B, Sharma L, Roberts L, Peng X, Bermejo S, Leighton I, Massana AC, Farhadian S, Ko AI, Cruz CSD, Bosio CM. Severe SARS-CoV-2 infection in humans is defined by a shift in the serum lipidome resulting in dysregulation of eicosanoid immune mediators. Research Square 2020 PMID: 32743565, PMCID: PMC7386513, DOI: 10.21203/rs.3.rs-42999/v1.
  • Differential effects of the Akt pathway on the internalization of Klebsiella by lung epithelium and macrophages.Chang, Feng J, Liu H, Liu W, Sharma L, Dela Cruz CS. Differential effects of the Akt pathway on the internalization of Klebsiella by lung epithelium and macrophages. Innate Immunity 2020, 26: 618-626. PMID: 32762278, PMCID: PMC7556185, DOI: 10.1177/1753425920942582.
  • Quantification of bronchoalveolar neutrophil extracellular traps and phagocytosis in murine pneumonia.Gautam S, Stahl Y, Young GM, Howell R, Cohen AJ, Tsang DA, Martin T, Sharma L, Dela Cruz CS. Quantification of bronchoalveolar neutrophil extracellular traps and phagocytosis in murine pneumonia. American Journal Of Physiology. Lung Cellular And Molecular Physiology 2020, 319: L661-L669. PMID: 32783617, PMCID: PMC7642899, DOI: 10.1152/ajplung.00316.2020.
  • Severe respiratory viral infection induces procalcitonin in the absence of bacterial pneumonia.Gautam S, Cohen AJ, Stahl Y, Valda Toro P, Young GM, Datta R, Yan X, Ristic NT, Bermejo SD, Sharma L, Restrepo M, Dela Cruz CS. Severe respiratory viral infection induces procalcitonin in the absence of bacterial pneumonia. Thorax 2020, 75: 974-981. PMID: 32826284, DOI: 10.1136/thoraxjnl-2020-214896.
  • Janus Kinase-Inhibitor and Type I Interferon Ability to Produce Favorable Clinical Outcomes in COVID-19 Patients: A Systematic Review and Meta-Analysis.Walz L, Cohen AJ, Rebaza AP, Vanchieri J, Slade MD, Dela Cruz CS, Sharma L. Janus Kinase-Inhibitor and Type I Interferon Ability to Produce Favorable Clinical Outcomes in COVID-19 Patients: A Systematic Review and Meta-Analysis. MedRxiv : The Preprint Server For Health Sciences 2020 PMID: 32817985, PMCID: PMC7430630, DOI: 10.1101/2020.08.10.20172189.
  • Rapid decline of seasonal influenza during the outbreak of COVID-19.Young G, Peng X, Rebeza A, Bermejo S, De C, Sharma L, Dela Cruz CS. Rapid decline of seasonal influenza during the outbreak of COVID-19. ERJ Open Research 2020, 6 PMID: 32832527, PMCID: PMC7430143, DOI: 10.1183/23120541.00296-2020.
  • Host tolerance contributes to persistent viral shedding in COVID-19.Chang, Chen Z, Wang FS, Xie LX, Dela Cruz CS, Sharma L, Qin EQ. Host tolerance contributes to persistent viral shedding in COVID-19. EClinicalMedicine 2020, 26: 100529. PMID: 32954242, PMCID: PMC7490255, DOI: 10.1016/j.eclinm.2020.100529.
  • JAK-Inhibitor and Type I Interferon Ability to Produce Favorable Clinical Outcomes in COVID-19 Patients: A Systematic Review and Meta-Analysis.Walz L, Cohen AJ, Rebaza AP, Vanchieri J, Slade MD, Cruz CSD, Sharma L. JAK-Inhibitor and Type I Interferon Ability to Produce Favorable Clinical Outcomes in COVID-19 Patients: A Systematic Review and Meta-Analysis. Research Square 2020 PMID: 32869016, PMCID: PMC7457585, DOI: 10.21203/rs.3.rs-64782/v1.
  • Does inflammation help during COVID-19?Sharma L, Chang, Dela Cruz CS. Does inflammation help during COVID-19? ERJ Open Research 2020, 6 PMID: 33083436, PMCID: PMC7553107, DOI: 10.1183/23120541.00557-2020.
  • The kinetics of humoral response and its relationship with the disease severity in COVID-19.Ren L, Zhang L, Chang, Wang J, Hu Y, Chen H, Guo L, Wu C, Wang C, Wang Y, Wang Y, Wang G, Yang S, Dela Cruz CS, Sharma L, Wang L, Zhang D, Wang J. The kinetics of humoral response and its relationship with the disease severity in COVID-19. Communications Biology 2020, 3: 780. PMID: 33311543, PMCID: PMC7733479, DOI: 10.1038/s42003-020-01526-8.
  • Identification of Potent and Safe Antiviral Therapeutic Candidates Against SARS-CoV-2.Xiao X, Wang C, Chang, Wang Y, Dong X, Jiao T, Zhao Z, Ren L, Dela Cruz CS, Sharma L, Lei X, Wang J. Identification of Potent and Safe Antiviral Therapeutic Candidates Against SARS-CoV-2. Frontiers In Immunology 2020, 11: 586572. PMID: 33324406, PMCID: PMC7723961, DOI: 10.3389/fimmu.2020.586572.
  • BPIFA1 regulates lung neutrophil recruitment and interferon signaling during acute inflammation.Britto CJ, Niu N, Khanal S, Huleihel L, Herazo-Maya J, Thompson A, Sauler M, Slade MD, Sharma L, Dela Cruz CS, Kaminski N, Cohn LE. BPIFA1 regulates lung neutrophil recruitment and interferon signaling during acute inflammation. American Journal Of Physiology. Lung Cellular And Molecular Physiology 2019, 316: L321-L333. PMID: 30461288, PMCID: PMC6397348, DOI: 10.1152/ajplung.00056.2018.
  • Teaching an Old Intensivist Neutrophil Tricks: Using Alveolar Neutrophilia to Diagnose Ventilator-associated Pneumonia.Gautam S, Sharma L, Dela Cruz CS. Teaching an Old Intensivist Neutrophil Tricks: Using Alveolar Neutrophilia to Diagnose Ventilator-associated Pneumonia. American Journal Of Respiratory And Critical Care Medicine 2019, 199: 1178-1181. PMID: 30543457, PMCID: PMC6519853, DOI: 10.1164/rccm.201811-2163ED.
  • Toll-Like Receptors 2 and 4 Modulate Pulmonary Inflammation and Host Factors Mediated by Outer Membrane Vesicles Derived from Acinetobacter baumannii.Marion CR, Lee J, Sharma L, Park KS, Lee C, Liu W, Liu P, Feng J, Gho YS, Dela Cruz CS. Toll-Like Receptors 2 and 4 Modulate Pulmonary Inflammation and Host Factors Mediated by Outer Membrane Vesicles Derived from Acinetobacter baumannii. Infection And Immunity 2019, 87 PMID: 31262980, PMCID: PMC6704609, DOI: 10.1128/IAI.00243-19.
  • When "B" becomes "A": the emerging threat of influenza B virus.Sharma L, Rebaza A, Dela Cruz CS. When "B" becomes "A": the emerging threat of influenza B virus. The European Respiratory Journal 2019, 54 PMID: 31416813, DOI: 10.1183/13993003.01325-2019.
  • A potential role of microvesicle-containing miR-223/142 in lung inflammation.Zhang D, Lee H, Wang X, Groot M, Sharma L, Dela Cruz CS, Jin Y. A potential role of microvesicle-containing miR-223/142 in lung inflammation. Thorax 2019, 74: 865-874. PMID: 31331947, PMCID: PMC7036165, DOI: 10.1136/thoraxjnl-2018-212994.
  • Mechanosensation of cyclical force by PIEZO1 is essential for innate immunity.Solis AG, Bielecki P, Steach HR, Sharma L, Harman CCD, Yun S, de Zoete MR, Warnock JN, To SDF, York AG, Mack M, Schwartz MA, Dela Cruz CS, Palm NW, Jackson R, Flavell RA. Mechanosensation of cyclical force by PIEZO1 is essential for innate immunity. Nature 2019, 573: 69-74. PMID: 31435009, PMCID: PMC6939392, DOI: 10.1038/s41586-019-1485-8.
  • Measurement of Chitinase Activity in Biological Samples.Amick AK, Liu Q, Gautam S, Chupp G, Dela Cruz CS, Sharma L. Measurement of Chitinase Activity in Biological Samples. Journal Of Visualized Experiments : JoVE 2019 PMID: 31498326, DOI: 10.3791/60159.
  • Author Correction: Mechanosensation of cyclical force by PIEZO1 is essential for innate immunity.Solis AG, Bielecki P, Steach HR, Sharma L, Harman CCD, Yun S, de Zoete MR, Warnock JN, To SDF, York AG, Mack M, Schwartz MA, Cruz CSD, Palm NW, Jackson R, Flavell RA. Author Correction: Mechanosensation of cyclical force by PIEZO1 is essential for innate immunity. Nature 2019, 575: E7. PMID: 31712625, DOI: 10.1038/s41586-019-1755-5.
  • Chitin and Its Effects on Inflammatory and Immune Responses.Elieh Ali Komi D, Sharma L, Dela Cruz CS. Chitin and Its Effects on Inflammatory and Immune Responses. Clinical Reviews In Allergy & Immunology 2018, 54: 213-223. PMID: 28251581, PMCID: PMC5680136, DOI: 10.1007/s12016-017-8600-0.
  • Innate Immunity of the Lung: From Basic Mechanisms to Translational Medicine.Hartl D, Tirouvanziam R, Laval J, Greene CM, Habiel D, Sharma L, Yildirim AÖ, Dela Cruz CS, Hogaboam CM. Innate Immunity of the Lung: From Basic Mechanisms to Translational Medicine. Journal Of Innate Immunity 2018, 10: 487-501. PMID: 29439264, PMCID: PMC6089674, DOI: 10.1159/000487057.
  • Regulation and Role of Chitotriosidase during Lung Infection with Klebsiella pneumoniae.Sharma L, Amick AK, Vasudevan S, Lee SW, Marion CR, Liu W, Brady V, Losier A, Bermejo SD, Britto CJ, Lee CG, Elias JA, Dela Cruz CS. Regulation and Role of Chitotriosidase during Lung Infection with Klebsiella pneumoniae. Journal Of Immunology (Baltimore, Md. : 1950) 2018, 201: 615-626. PMID: 29891554, PMCID: PMC6291403, DOI: 10.4049/jimmunol.1701782.
  • Impact of Cigarette Smoke Exposure on the Lung Fibroblastic Response after Influenza Pneumonia.Lee SW, Sharma L, Kang YA, Kim SH, Chandrasekharan S, Losier A, Brady V, Bermejo S, Andrews N, Yoon CM, Liu W, Lee JY, Kang MJ, Dela Cruz CS. Impact of Cigarette Smoke Exposure on the Lung Fibroblastic Response after Influenza Pneumonia. American Journal Of Respiratory Cell And Molecular Biology 2018, 59: 770-781. PMID: 30110182, PMCID: PMC6293077, DOI: 10.1165/rcmb.2018-0004OC.
  • Regional Differences in Airway Epithelial Cells Reveal Tradeoff between Defense against Oxidative Stress and Defense against Rhinovirus.Mihaylova VT, Kong Y, Fedorova O, Sharma L, Dela Cruz CS, Pyle AM, Iwasaki A, Foxman EF. Regional Differences in Airway Epithelial Cells Reveal Tradeoff between Defense against Oxidative Stress and Defense against Rhinovirus. Cell Reports 2018, 24: 3000-3007.e3. PMID: 30208323, PMCID: PMC6190718, DOI: 10.1016/j.celrep.2018.08.033.
  • A Slick Solution to a Sticky Problem.Gautam S, Sharma L, Dela Cruz CS, Spiegel DA. A Slick Solution to a Sticky Problem. Biochemistry 2018, 57: 5923-5924. PMID: 30289246, DOI: 10.1021/acs.biochem.8b00916.
  • The fungal ligand chitin directly binds TLR2 and triggers inflammation dependent on oligomer size.Fuchs K, Cardona Gloria Y, Wolz OO, Herster F, Sharma L, Dillen CA, Täumer C, Dickhöfer S, Bittner Z, Dang TM, Singh A, Haischer D, Schlöffel MA, Koymans KJ, Sanmuganantham T, Krach M, Roger T, Le Roy D, Schilling NA, Frauhammer F, Miller LS, Nürnberger T, LeibundGut-Landmann S, Gust AA, Macek B, Frank M, Gouttefangeas C, Dela Cruz CS, Hartl D, Weber AN. The fungal ligand chitin directly binds TLR2 and triggers inflammation dependent on oligomer size. EMBO Reports 2018, 19 PMID: 30337494, PMCID: PMC6280652, DOI: 10.15252/embr.201846065.
  • Personalizing the Management of Pneumonia.Gautam S, Sharma L, Dela Cruz CS. Personalizing the Management of Pneumonia. Clinics In Chest Medicine 2018, 39: 871-900. PMID: 30390755, DOI: 10.1016/j.ccm.2018.08.008.
  • The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients.Kim HJ, Kim YS, Kim KH, Choi JP, Kim YK, Yun S, Sharma L, Dela Cruz CS, Lee JS, Oh YM, Lee SD, Lee SW. The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients. Experimental & Molecular Medicine 2017, 49: e316. PMID: 28408748, PMCID: PMC5420800, DOI: 10.1038/emm.2017.7.
  • Atypical Pneumonia: Updates on Legionella, Chlamydophila, and Mycoplasma Pneumonia.Sharma L, Losier A, Tolbert T, Dela Cruz CS, Marion CR. Atypical Pneumonia: Updates on Legionella, Chlamydophila, and Mycoplasma Pneumonia. Clinics In Chest Medicine 2017, 38: 45-58. PMID: 28159161, PMCID: PMC5679202, DOI: 10.1016/j.ccm.2016.11.011.
  • IL-13Rα2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses.Lee CM, He CH, Nour AM, Zhou Y, Ma B, Park JW, Kim KH, Cruz CD, Sharma L, Nasr ML, Modis Y, Lee CG, Elias JA. IL-13Rα2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses. Nature Communications 2016, 7: 12752. PMID: 27629921, PMCID: PMC5027616, DOI: 10.1038/ncomms12752.
  • Assessment of phagocytic activity of cultured macrophages using fluorescence microscopy and flow cytometry.Sharma L, Wu W, Dholakiya SL, Gorasiya S, Wu J, Sitapara R, Patel V, Wang M, Zur M, Reddy S, Siegelaub N, Bamba K, Barile FA, Mantell LL. Assessment of phagocytic activity of cultured macrophages using fluorescence microscopy and flow cytometry. Methods In Molecular Biology (Clifton, N.J.) 2014, 1172: 137-45. PMID: 24908301, DOI: 10.1007/978-1-4939-0928-5_12.
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