Lesley Devine, PhD
Technical Director Yale Flow Core Facility; Scientific Director, Cancer Center Immune Monitoring Laboratory
Research & Publications
Biography
Research Summary
The main goal of the lab is in monitoring the cellular immune response to determine the effects on the immune system of treatments with novel immune therapies.Current trials that the core is involved with include anti-CD3 treatment for diabetes and angiogenesis inhibitors and immunomodulatory reagents such as anti-CTLA-4, anti-PD-1 and IL-2 in the treatment of melanoma. 6-color flow cytometry is used for phenotypic analysis of the different immune cell populations, including the use of MHC class I tetramers to measure the antigen-specific response.Functional assays include the use of Luminex, a bead-based technology for multiplex cytokine analysis and flow-based cytotoxicity assays.
Specialized Terms: Effects of novel immune therapies on the immune system
Coauthors
Research Interests
Diabetes Mellitus; Immune System; Melanoma; Medical Laboratory Science; Angiogenesis Inhibitors
Selected Publications
- Quantitative DNA Repair Biomarkers and Immune Profiling for Temozolomide and Olaparib in Metastatic Colorectal CancerCecchini M, Zhang J, Wei W, Sklar J, Lacy J, Zhong M, Kong Y, Zhao H, DiPalermo J, Devine L, Stein S, Kortmansky J, Johung K, Bindra R, LoRusso P, Schalper K. Quantitative DNA Repair Biomarkers and Immune Profiling for Temozolomide and Olaparib in Metastatic Colorectal Cancer. Cancer Research Communications 2023, 3: 1132-1139. PMID: 37387791, PMCID: PMC10305782, DOI: 10.1158/2767-9764.crc-23-0045.
- Metabolomic and transcriptomic signatures of influenza vaccine response in healthy young and older adultsChou C, Mohanty S, Kang HA, Kong L, Avila‐Pacheco J, Joshi SR, Ueda I, Devine L, Raddassi K, Pierce K, Jeanfavre S, Bullock K, Meng H, Clish C, Santori FR, Shaw AC, Xavier RJ. Metabolomic and transcriptomic signatures of influenza vaccine response in healthy young and older adults. Aging Cell 2022, 21: e13682. PMID: 35996998, PMCID: PMC9470889, DOI: 10.1111/acel.13682.
- Elevated Levels of CD64 MFI on Monocyte Subsets Are Associated with a History of Stroke in Sickle Cell DiseaseCurtis S, Balbuena-Merle R, Devine L, Zelterman D, Roberts J, Dearborn-Tomazos J, Sansing L, Hendrickson J. Elevated Levels of CD64 MFI on Monocyte Subsets Are Associated with a History of Stroke in Sickle Cell Disease. Blood 2018, 132: 1093. DOI: 10.1182/blood-2018-99-117504.
- Differential Expression of the Human CD8β Splice Variants and Regulation of the M-2 Isoform by UbiquitinationThakral D, Dobbins J, Devine L, Kavathas PB. Differential Expression of the Human CD8β Splice Variants and Regulation of the M-2 Isoform by Ubiquitination. The Journal Of Immunology 2008, 180: 7431-7442. PMID: 18490743, DOI: 10.4049/jimmunol.180.11.7431.
- Differential Expression of Human CD8 Beta‐chain Isoforms and Regulation of M‐2 Isoform (splice variant 1) by UbiquitinationThakral D, Dobbins J, Devine L, Kavathas P. Differential Expression of Human CD8 Beta‐chain Isoforms and Regulation of M‐2 Isoform (splice variant 1) by Ubiquitination. The FASEB Journal 2008, 22: 367-367. DOI: 10.1096/fasebj.22.2_supplement.367.
- Mapping the Binding Site on CD8β for MHC Class I Reveals Mutants with Enhanced BindingDevine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB. Mapping the Binding Site on CD8β for MHC Class I Reveals Mutants with Enhanced Binding. The Journal Of Immunology 2006, 177: 3930-3938. PMID: 16951356, DOI: 10.4049/jimmunol.177.6.3930.
- Location of the epitope for an anti-CD8α antibody 53.6.7 which enhances CD8α-MHC class I interaction indicates antibody stabilization of a higher affinity CD8 conformationDevine L, Hodsdon ME, Daniels MA, Jameson SC, Kavathas PB. Location of the epitope for an anti-CD8α antibody 53.6.7 which enhances CD8α-MHC class I interaction indicates antibody stabilization of a higher affinity CD8 conformation. Immunology Letters 2004, 93: 123-130. PMID: 15158607, DOI: 10.1016/j.imlet.2004.02.002.
- The Complementarity-Determining Region-Like Loops of CD8α Interact Differently with β2-Microglobulin of the Class I Molecules H-2Kb and Thymic Leukemia Antigen, While Similarly with Their α3 DomainsDevine L, Rogozinski L, Naidenko OV, Cheroutre H, Kavathas PB. The Complementarity-Determining Region-Like Loops of CD8α Interact Differently with β2-Microglobulin of the Class I Molecules H-2Kb and Thymic Leukemia Antigen, While Similarly with Their α3 Domains. The Journal Of Immunology 2002, 168: 3881-3886. PMID: 11937542, DOI: 10.4049/jimmunol.168.8.3881.
- A Non-class I MHC Intestinal Epithelial Surface Glycoprotein, gp180, Binds to CD8Campbell N, Park M, Toy L, Yio X, Devine L, Kavathas P, Mayer L. A Non-class I MHC Intestinal Epithelial Surface Glycoprotein, gp180, Binds to CD8. Clinical Immunology 2002, 102: 267-274. PMID: 11890713, DOI: 10.1006/clim.2001.5170.
- CD8 Binding to MHC Class I Molecules Is Influenced by T Cell Maturation and GlycosylationDaniels M, Devine L, Miller J, Moser J, Lukacher A, Altman J, Kavathas P, Hogquist K, Jameson S. CD8 Binding to MHC Class I Molecules Is Influenced by T Cell Maturation and Glycosylation. Immunity 2001, 15: 1051-1061. PMID: 11754824, DOI: 10.1016/s1074-7613(01)00252-7.
- Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I TetramersKim S, Devine L, Angevine M, DeMars R, Kavathas P. Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers. The Journal Of Immunology 2000, 165: 7285-7292. PMID: 11120863, DOI: 10.4049/jimmunol.165.12.7285.
- Human CD8β, But Not Mouse CD8β, Can Be Expressed in the Absence of CD8α as a ββ HomodimerDevine L, Kieffer L, Aitken V, Kavathas P. Human CD8β, But Not Mouse CD8β, Can Be Expressed in the Absence of CD8α as a ββ Homodimer. The Journal Of Immunology 2000, 164: 833-838. PMID: 10623829, DOI: 10.4049/jimmunol.164.2.833.
- Molecular analysis of protein interactions mediating the function of the cell surface protein CD8Devine L, Kavathas P. Molecular analysis of protein interactions mediating the function of the cell surface protein CD8. Immunologic Research 1999, 19: 201-210. PMID: 10493174, DOI: 10.1007/bf02786488.
- Orientation of the Ig domains of CD8 alpha beta relative to MHC class I.Devine L, Sun J, Barr M, Kavathas P. Orientation of the Ig domains of CD8 alpha beta relative to MHC class I. The Journal Of Immunology 1999, 162: 846-51. PMID: 9916707, DOI: 10.4049/jimmunol.162.2.846.
- SV40 large T immortalised cell lines of the rat blood-brain and blood-retinal barriers retain their phenotypic and immunological characteristicsGreenwood J, Pryce G, Devine L, Male D, dos Santos W, Calder V, Adamson P. SV40 large T immortalised cell lines of the rat blood-brain and blood-retinal barriers retain their phenotypic and immunological characteristics. Journal Of Neuroimmunology 1996, 71: 51-63. PMID: 8982103, DOI: 10.1016/s0165-5728(96)00130-0.
- Role of LFA‐1, ICAM‐1, VLA‐4 and VCAM‐1 in lymphocyte migration across retinal pigment epithelial monolayers in vitroDEVINE L, LIGHTMAN S, GREENWOOD J. Role of LFA‐1, ICAM‐1, VLA‐4 and VCAM‐1 in lymphocyte migration across retinal pigment epithelial monolayers in vitro. Immunology 1996, 88: 456-462. PMID: 8774365, PMCID: PMC1456340, DOI: 10.1046/j.1365-2567.1996.d01-666.x.
- Lymphocyte Migration across the Anterior and Posterior Blood–Retinal Barrierin VitroDevine L, Lightman S, Greenwood J. Lymphocyte Migration across the Anterior and Posterior Blood–Retinal Barrierin Vitro. Cellular Immunology 1996, 168: 267-275. PMID: 8640874, DOI: 10.1006/cimm.1996.0075.
- A Comparison of Lymphocyte Migration Across the Anterior and Posterior Blood-Retinal Barrier in VitroDevine L, Lightman S, Greenwood J. A Comparison of Lymphocyte Migration Across the Anterior and Posterior Blood-Retinal Barrier in Vitro. 1996, 46: 245-251. DOI: 10.1007/978-1-4757-9489-2_41.
- The Blood-Retinal Barrier in Immune-Mediated Diseases of the RetinaGreenwood J, Bamforth S, Wang Y, Devine L. The Blood-Retinal Barrier in Immune-Mediated Diseases of the Retina. 1995, 315-326. DOI: 10.1007/978-1-4899-1054-7_31.