Research & Publications
Our overarching goal is to develop quantitative molecular platforms to detect and identify molecular interactomes of membrane proteins and determine their hierarchical organisation directly from the cellular environment with precise spatial and molecular resolution. To achieve this we exploit the cross-disciplinary expertise of our lab and combine nativeMS with a range of orthogonal quantitative approaches. Together, they provide unique quantitative tools to study membrane biology. Research in our lab spans from designing fundamentally novel experimental platform to studying specific membrane associated signaling pathways. Our special emphasis lies in understanding neural signaling and associated impairment in neurodegenerative diseases. Some of the key biological questions we are currently addressing are:
(i) How hierarchical organization of proteins and lipids at the synaptic vesicles regulate fast Ca2+ mediated release of neurotransmitter at the neuronal synapses?
(ii) How trafficking of lipids and proteins at the membrane contact site of the cellular organelles regulate cellular homeostasis and their impairments lead to different neurocognitive disorders?
We work closely with researchers with varied expertise. While we love the physicists and the chemists who develop novel experimental tools, we cannot live without the biologists and the medics who use these cutting-edge techniques to solve the problems most pertinent to humankind. We even have an anthropologist. We are always interested in innovators, the creative thinkers, the dreamers, and the doers.
If you want to discover the world of native mass spectrometry, we want to hear from you.
If you have ideas, we want to hear from you.
If you are merely curious, we want to hear from you.
We currently have multiple openings for postdocs, graduate students and research assistants. Contact me directly with your CV and research interests. Click the lab website link for more details
For further details visit: https://www.theguptalab.com/
Selected relevant publications:
- Gupta K, Li J, Liko I, Gault J, Bechara C, Wu D, Hopper JTS, Giles K, Benesch JLP, Robinson CV. Identifying key membrane protein lipid interactions using mass spectrometry. Nature Protocol, 2018:13:1106
- Ambrose S, Housden NG, Gupta K, Fan J, White P, Yen H, Marcoux J, Kleanthous C, Hopper JTS, Robinson CV. Native desorption electrospray ionization liberates soluble and membrane protein complexes from surfaces Angew. Chem. Int. Ed. (published online, doi: 10.1002/anie.201704849)
- Gupta K, Donlan JAC, Hopper JTS, Uzdavinys P, Landreh M, Struwe WB, Drew D, Baldwin AJ, Phillip J. Stansfeld PJ, Robinson CV. The role of interfacial lipids in stabilizing membrane protein oligomers. Nature 2017:541: 421
- Landreh M, Marklund EG, Uzdavinys P, Degiocomi MT, Gault J, Coincon M, Liko I, Gupta K, Benesch JLP, Drew David, Robinson CV. Integrating mass spectrometry with MD simulations reveals the role of lipids in Na+/H+ antiporters. Nature Comm. 2017:8: 13993
- Gault J, Donlan JAC, Liko I, Hopper JTS, Gupta K, Housden NG, Struwe WB, Marty MT, Mize T, Bechara C, Zhu Y, Wu B, Kleanthous C, Belov M, Damoc E, Makarov A, Robinson CV. High-resolution mass spectrometry of small molecules bound to membrane proteins. Nature Methods 2016:13: 336
Biophysics; Cell Membrane; Cell Membrane Permeability; Mass Spectrometry; Membrane Transport Proteins; Chemicals and Drugs