2018
T cell LFA-1-induced proinflammatory mRNA stabilization is mediated by the p38 pathway kinase MK2 in a process regulated by hnRNPs C, H1 and K
Rao GK, Wong A, Collinge M, Sarhan J, Yarovinsky TO, Ramgolam VS, Gaestel M, Pardi R, Bender JR. T cell LFA-1-induced proinflammatory mRNA stabilization is mediated by the p38 pathway kinase MK2 in a process regulated by hnRNPs C, H1 and K. PLOS ONE 2018, 13: e0201103. PMID: 30048492, PMCID: PMC6065199, DOI: 10.1371/journal.pone.0201103.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Culture TechniquesCytoplasmELAV-Like Protein 1Heterogeneous-Nuclear RibonucleoproteinsHumansIntracellular Signaling Peptides and ProteinsJurkat CellsLymphocyte Function-Associated Antigen-1Mice, Inbred C57BLMice, KnockoutProtein Serine-Threonine KinasesProteomeRNA StabilityRNA, MessengerSignal TransductionT-LymphocytesConceptsKinase MK2Β2-integrin lymphocyte function-associated antigen-1AU-rich elementsLymphocyte function-associated antigen-1Integrin lymphocyte function-associated antigen-1HuR localizationProtein HuR.Key regulatorMRNA stabilizationCritical activatorCytoplasmic translocationHuR activitySequential activationHuRIntricate processFunction-associated antigen-1MRNAEngagement resultsMK2Antigen 1H1ActivationHnRNPsHuR.Transcripts
2012
Macrophage β2 Integrin–Mediated, HuR-Dependent Stabilization of Angiogenic Factor–Encoding mRNAs in Inflammatory Angiogenesis
Zhang J, Modi Y, Yarovinsky T, Yu J, Collinge M, Kyriakides T, Zhu Y, Sessa WC, Pardi R, Bender JR. Macrophage β2 Integrin–Mediated, HuR-Dependent Stabilization of Angiogenic Factor–Encoding mRNAs in Inflammatory Angiogenesis. American Journal Of Pathology 2012, 180: 1751-1760. PMID: 22322302, PMCID: PMC3349897, DOI: 10.1016/j.ajpath.2011.12.025.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenesis Inducing AgentsAnimalsCD18 AntigensCell AdhesionCells, CulturedDisease Models, AnimalELAV ProteinsGene Expression RegulationGene Knockout TechniquesHindlimbInflammationIschemiaMacrophagesMiceMice, KnockoutMuscle, SkeletalNeovascularization, PathologicReal-Time Polymerase Chain ReactionRNA, MessengerConceptsKnockout miceAngiogenic factorsT cell cytokine productionIntercellular adhesion molecule-1Blood flow recoveryFemoral artery ligationLittermate wild-type controlsVascular endothelial growth factorBone marrow-derived macrophagesMatrix metalloproteinase-9Adhesion molecule-1Endothelial growth factorMarrow-derived macrophagesSoluble factor productionWild-type controlsArtery ligationLigand intercellular adhesion molecule-1Cytokine productionInflammatory angiogenesisMetalloproteinase-9Tissue ischemiaInflammatory stimuliMolecule-1Macrophage productionNeovascular response
2008
Targeted inactivation of the COP9 signalosome impairs multiple stagesof T cell development
Panattoni M, Sanvito F, Basso V, Doglioni C, Casorati G, Montini E, Bender JR, Mondino A, Pardi R. Targeted inactivation of the COP9 signalosome impairs multiple stagesof T cell development. Journal Of Experimental Medicine 2008, 205: 465-477. PMID: 18268034, PMCID: PMC2271025, DOI: 10.1084/jem.20070725.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBcl-X ProteinCell CycleCell LineCell ProliferationCOP9 Signalosome ComplexCyclin-Dependent Kinase Inhibitor p16DNA RepairFemaleHomeodomain ProteinsIntracellular Signaling Peptides and ProteinsMiceMice, Inbred C57BLMice, KnockoutMultiprotein ComplexesNF-kappa BPeptide HydrolasesProto-Oncogene Proteins c-bcl-2Receptors, Antigen, T-CellRNA, MessengerT-LymphocytesTumor Suppressor Protein p53Ubiquitin-Protein LigasesConceptsCOP9 signalosomeCSN5/Jab1Genetic programBcl-2 family membersGenetic complementation analysisBcl-xL/BclS-phase progressionDistinct developmental stagesCell cycle progressionT cell developmentComplementation analysisLower organismsCatalytic subunitPositive selectionTranscription factorsDNA repairCycle progressionCell developmentThymocyte survivalDevelopmental stagesNF-kappaB pathwayTransgenic backgroundPhase progressionRapid turnoverEffector molecules
2007
COP1D, an alternatively spliced constitutive photomorphogenic-1 (COP1) product, stabilizes UV stress-induced c-Jun through inhibition of full-length COP1
Savio M, Rotondo G, Maglie S, Rossetti G, Bender J, Pardi R. COP1D, an alternatively spliced constitutive photomorphogenic-1 (COP1) product, stabilizes UV stress-induced c-Jun through inhibition of full-length COP1. Oncogene 2007, 27: 2401-2411. PMID: 17968316, DOI: 10.1038/sj.onc.1210892.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBreast NeoplasmsCell LineDown-RegulationEnzyme StabilityHealthHeLa CellsHumansIsoenzymesJNK Mitogen-Activated Protein KinasesKineticsMolecular Sequence DataProteasome Endopeptidase ComplexRNA, MessengerTranscription, GeneticTumor Suppressor Protein p53Ubiquitin-Protein LigasesConceptsFull-length proteinC-JunCullin-RING ligase complexRING finger ubiquitin ligaseProteasome-dependent degradationDominant-negative functionCoiled-coil regionFunction of COP1Post-translational changesP53 protein levelsLigase complexCRL complexesE3 ligasesE3 ligaseUbiquitin ligaseCOP1Ectopic expressionRNA interferenceUV stressElevated c-JunPolyubiquitinationSplice variants
2006
LFA-1-Dependent HuR Nuclear Export and Cytokine mRNA Stabilization in T Cell Activation
Wang JG, Collinge M, Ramgolam V, Ayalon O, Fan XC, Pardi R, Bender JR. LFA-1-Dependent HuR Nuclear Export and Cytokine mRNA Stabilization in T Cell Activation. The Journal Of Immunology 2006, 176: 2105-2113. PMID: 16455966, DOI: 10.4049/jimmunol.176.4.2105.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAntigens, SurfaceBase SequenceCD28 AntigensCell NucleusCells, CulturedCytokinesCytoplasmELAV ProteinsELAV-Like Protein 1HumansLymphocyte ActivationLymphocyte Function-Associated Antigen-1Molecular Sequence DataRNA StabilityRNA, MessengerRNA, Small InterferingRNA-Binding ProteinsT-LymphocytesConceptsNuclear exportAU-rich element (ARE) sequenceMRNA stabilizationClass II AU-rich elementsT cell activationAU-rich elementsLymphokine gene expressionRapid nuclearRegulated processIntegrin engagementCell activationMRNA reporterRNA interferenceHuR functionIntegrin LFA-1MRNA stabilityGene expressionProtein productionHuman peripheral T cellsCytoplasmic translocationGM-CSF mRNAElement sequencesProtein HuRHuR levelsLFA-1
2003
Plasma membrane localization and function of the estrogen receptor α variant (ER46) in human endothelial cells
Li L, Haynes MP, Bender JR. Plasma membrane localization and function of the estrogen receptor α variant (ER46) in human endothelial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2003, 100: 4807-4812. PMID: 12682286, PMCID: PMC153637, DOI: 10.1073/pnas.0831079100.Peer-Reviewed Original ResearchMeSH KeywordsAlternative SplicingAnimalsBase SequenceCell LineCell MembraneCOS CellsEndothelium, VascularEstrogen Receptor alphaGenes, ReporterGenetic VariationHumansNitric Oxide SynthaseNitric Oxide Synthase Type IIIProtein Processing, Post-TranslationalReceptors, EstrogenRecombinant ProteinsRNA, MessengerSubcellular FractionsTranscriptional ActivationTransfectionConceptsPlasma membranePalmitoylation-dependent mannerPlasma membrane localizationInhibition of palmitoylationFull-length ER alphaReporter gene transactivationCOS-7 cellsEndothelial cell proteinsMembrane localizationAlternative splicingEstrogen receptor α variantsOestrogen receptor-alpha variantsN-terminusHuman endothelial cellsCell proteinsER46Synthase pathwayAcid labelingHy926 cellsNonendothelial cellsENOS activationENOS phosphorylationCellsEndothelial cellsMembrane
2000
Simvastatin Modulates Cytokine-Mediated Endothelial Cell Adhesion Molecule Induction: Involvement of an Inhibitory G Protein
Sadeghi M, Collinge M, Pardi R, Bender J. Simvastatin Modulates Cytokine-Mediated Endothelial Cell Adhesion Molecule Induction: Involvement of an Inhibitory G Protein. The Journal Of Immunology 2000, 165: 2712-2718. PMID: 10946302, DOI: 10.4049/jimmunol.165.5.2712.Peer-Reviewed Original ResearchMeSH KeywordsAdjuvants, ImmunologicBiological TransportCell Adhesion MoleculesCells, CulturedCholesterolCytokinesDrug SynergismE-SelectinEndothelium, VascularGene Expression RegulationGTP-Binding Protein alpha Subunits, Gi-GoHumansHydroxymethylglutaryl-CoA Reductase InhibitorsIntercellular Adhesion Molecule-1Interleukin-1NF-kappa BRNA, MessengerSignal TransductionSimvastatinSodium FluorideUmbilical VeinsVascular Cell Adhesion Molecule-1ConceptsEffect of simvastatinCytokine-induced expressionIL-1Endothelial CAMsEndothelial cell adhesion molecules E-selectinNF-kappaBProinflammatory cytokines IL-1Cell adhesion molecules E-selectinAdhesion molecules E-selectinPotent immune modulatorG protein activator NaFCytokines IL-1G protein-coupled pathwayInhibitory G proteinCytokine-mediated activationSelectin mRNA levelsBasal toneProinflammatory cytokinesGialpha proteinsImmune modulatorsTNF-alphaICAM-1Pertussis toxinE-selectinEndothelial responseCD28 and LFA‐1 contribute to cyclosporin A‐resistant T cell growth by stabilizing the IL‐2 mRNA through distinct signaling pathways
Geginat J, Clissi B, Moro M, Dellabona P, Bender J, Pardi R. CD28 and LFA‐1 contribute to cyclosporin A‐resistant T cell growth by stabilizing the IL‐2 mRNA through distinct signaling pathways. European Journal Of Immunology 2000, 30: 1136-1144. PMID: 10760803, DOI: 10.1002/(sici)1521-4141(200004)30:4<1136::aid-immu1136>3.0.co;2-3.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDB7-2 AntigenCalcineurinCD28 AntigensCells, CulturedCyclosporineCytoskeletonDendritic CellsDNA-Binding ProteinsDrug SynergismHumansIntercellular Adhesion Molecule-1Interleukin-2Lymphocyte ActivationLymphocyte Function-Associated Antigen-1Membrane GlycoproteinsMitogen-Activated Protein KinasesNF-kappa BNFATC Transcription FactorsNuclear ProteinsPromoter Regions, GeneticProtein BindingRNA StabilityRNA, MessengerSignal TransductionSuperantigensT-LymphocytesTranscription FactorsConceptsIL-2 mRNALFA-1ICAM-1IL-2 dependentT cell proliferationSubsequent T cell proliferationCostimulatory molecule CD28TCR-induced proliferationSignaling pathwaysT cell growthIL-2 transcriptsGraft rejectionDendritic cellsIL-2Clinical transplantationT lymphocytesMolecule CD28Primary T lymphocytesNF-kappaBCD28Distinct signaling pathwaysLower transcriptional rateDifferent signaling pathwaysProtein kinase activationCell proliferation
1998
Class II transactivator-independent endothelial cell MHC class II gene activation induced by lymphocyte adhesion.
Collinge M, Pardi R, Bender J. Class II transactivator-independent endothelial cell MHC class II gene activation induced by lymphocyte adhesion. The Journal Of Immunology 1998, 161: 1589-93. PMID: 9712019, DOI: 10.4049/jimmunol.161.4.1589.Peer-Reviewed Original ResearchConceptsClass II transactivatorNK cellsIFN-gamma-dependent mechanismIFN-gamma-independent mannerLymphocyte adhesionMHC class II expressionHLA-DR alpha mRNAHLA-DR expressionMHC class II moleculesNK cell activationAllogeneic endothelial cellsClass II expressionIFN-gamma-induced MHC class II expressionClass II moleculesAlpha mRNA expressionCell linesHLA-DRIFN-gammaCell activationMRNA expressionEndothelial cellsAlpha mRNACellsPromoter constructsActivationPosttranscriptional regulation of urokinase plasminogen activator receptor messenger RNA levels by leukocyte integrin engagement
Wang G, Collinge M, Blasi F, Pardi R, Bender J. Posttranscriptional regulation of urokinase plasminogen activator receptor messenger RNA levels by leukocyte integrin engagement. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 6296-6301. PMID: 9600959, PMCID: PMC27663, DOI: 10.1073/pnas.95.11.6296.Peer-Reviewed Original ResearchConceptsAU-rich elementsLFA-1 activationMRNA degradationReporter constructsUrokinase plasminogen activator receptorT cell activationCrucial cis-acting elementsCis-acting elementsLymphocyte function-associated antigen-1Integrin lymphocyte function-associated antigen-1Jurkat T cellsLFA-1 engagementUPAR cDNAReceptor-mediated signalingPosttranscriptional regulationTransmembrane signalingReporter mRNAIntegrin engagementCell activationInhibitor 5Adhesion receptorsSecond messengerMessenger RNA levelsPlasminogen activator receptorFunction-associated antigen-1
1996
Integrin-dependent induction of functional urokinase receptors in primary T lymphocytes.
Bianchi E, Ferrero E, Fazioli F, Mangili F, Wang J, Bender J, Blasi F, Pardi R. Integrin-dependent induction of functional urokinase receptors in primary T lymphocytes. Journal Of Clinical Investigation 1996, 98: 1133-1141. PMID: 8787676, PMCID: PMC507535, DOI: 10.1172/jci118896.Peer-Reviewed Original ResearchMeSH KeywordsAdenylyl CyclasesBreast NeoplasmsCD18 AntigensCell MovementCyclic AMPFemaleFlow CytometryHumansImmunologic CappingIntegrin beta1Lymphocyte ActivationLymphocytes, Tumor-InfiltratingPancreatic NeoplasmsProtein Kinase CReceptors, Cell SurfaceReceptors, Urokinase Plasminogen ActivatorRNA, MessengerSecond Messenger SystemsT-LymphocytesUrokinase-Type Plasminogen ActivatorConceptsUrokinase plasminogen activatorAntigen receptor complexProtein kinase C activationAnti-uPAR antibodiesPlasminogen activator/plasmin systemKinase C activationPrimary human tumor specimensRegulated processPrimary T lymphocytesHuman tumor specimensT lymphocytesT cellsUrokinase receptorCell migrationExtracellular matrixReceptor complexC activationProtein levelsSites of inflammationT cell migrationPlasminogen activationCatalytic activationIntracellular cyclic AMPPlasmin systemCyclic AMPTumor necrosis factor alpha-induced vascular leakage involves PECAM1 phosphorylation.
Ferrero E, Villa A, Ferrero M, Toninelli E, Bender J, Pardi R, Zocchi M. Tumor necrosis factor alpha-induced vascular leakage involves PECAM1 phosphorylation. Cancer Research 1996, 56: 3211-5. PMID: 8764109.Peer-Reviewed Original ResearchMeSH KeywordsAlkaloidsAntigens, Differentiation, MyelomonocyticBenzoquinonesCapillary PermeabilityCell Adhesion MoleculesCell CompartmentationCells, CulturedEndothelium, VascularEnzyme InhibitorsFluorescent Antibody Technique, IndirectGene ExpressionHumansLactams, MacrocyclicPhosphorylationPlatelet Endothelial Cell Adhesion Molecule-1Protein Kinase InhibitorsQuinonesRifabutinRNA, MessengerStaurosporineTumor Necrosis Factor-alphaUmbilical VeinsConceptsVascular leakageEndothelial cellsNecrosis factor alphaTyrosine kinase inhibitorsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsNatural tyrosine kinase inhibitorSerine-threonine kinaseVein endothelial cellsSurface redistributionFactor alphaVascular permeabilityVivo administrationKinase inhibitorsCytoskeletal associationMouse liverLavendustin AUse of inhibitorsPhosphorylationInhibitorsEffects of 17beta-estradiol on cytokine-induced endothelial cell adhesion molecule expression.
Caulin-Glaser T, Watson C, Pardi R, Bender J. Effects of 17beta-estradiol on cytokine-induced endothelial cell adhesion molecule expression. Journal Of Clinical Investigation 1996, 98: 36-42. PMID: 8690801, PMCID: PMC507398, DOI: 10.1172/jci118774.Peer-Reviewed Original ResearchConceptsCultured human umbilical vein ECEndothelial cell adhesion molecule expressionVascular cell adhesion molecule-1 inductionEstrogen receptor-mediated effectsCardiovascular protective roleCell adhesion molecule expressionAge-matched malesIncidence of atherosclerosisReceptor-mediated effectsAdhesion molecule expressionEndothelial cell activationHuman umbilical vein ECSimilar risk profilesSelectin mRNA levelsIL-1 activationPremenopausal womenAntagonist ICIMolecule expressionMononuclear leukocytesE-selectinChemotactic factorsCytokines resultsProtective roleCell activationLeukocyte adhesion
1994
Heterogeneous activation thresholds to cytokines in genetically distinct endothelial cells: evidence for diverse transcriptional responses.
Bender JR, Sadeghi MM, Watson C, Pfau S, Pardi R. Heterogeneous activation thresholds to cytokines in genetically distinct endothelial cells: evidence for diverse transcriptional responses. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 3994-3998. PMID: 7513430, PMCID: PMC43709, DOI: 10.1073/pnas.91.9.3994.Peer-Reviewed Original Research