Hesper Rego, PhD
Associate Professor Term
Research & Publications
Biography
News
Locations
Research Summary
The Rego Lab uses a variety of techniques to investigate how individual bacteria create and maintain non-genetic sources of variability. This is an important survival mechanism for many bacteria and appears especially important for the pathogen Mycobacterium tuberculosis. We use advanced imaging technologies, including super-resolution microscopy, fluorescent biosensors, high-throughput genetics, and traditional molecular biology to study mycobacterial heterogeneity on a single-cell level.
Coauthors
Research Interests
Bacteria; Cell Biology; Imaging, Three-Dimensional; Single-Cell Analysis
Selected Publications
- An essential periplasmic protein coordinates lipid trafficking and is required for asymmetric polar growth in mycobacteriaGupta K, Gwin C, Rahlwes K, Biegas K, Wang C, Park J, Liu J, Swarts B, Morita Y, Rego E. An essential periplasmic protein coordinates lipid trafficking and is required for asymmetric polar growth in mycobacteria. ELife 2022, 11: e80395. PMID: 36346214, PMCID: PMC9678360, DOI: 10.7554/elife.80395.
- Itaconate is an effector of a Rab GTPase cell-autonomous host defense pathway against SalmonellaChen M, Sun H, Boot M, Shao L, Chang SJ, Wang W, Lam TT, Lara-Tejero M, Rego EH, Galán JE. Itaconate is an effector of a Rab GTPase cell-autonomous host defense pathway against Salmonella. Science 2020, 369: 450-455. PMID: 32703879, PMCID: PMC8020367, DOI: 10.1126/science.aaz1333.
- Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shapeBaranowski C, Welsh M, Sham L, Eskandarian H, Lim H, Kieser K, Wagner J, McKinney J, Fantner G, Ioerger T, Walker S, Bernhardt T, Rubin E, Rego E. Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape. ELife 2018, 7: e37516. PMID: 30324906, PMCID: PMC6231781, DOI: 10.7554/elife.37516.
- Deletion of a mycobacterial divisome factor collapses single-cell phenotypic heterogeneityRego E, Audette R, Rubin E. Deletion of a mycobacterial divisome factor collapses single-cell phenotypic heterogeneity. Nature 2017, 546: 153-157. PMID: 28569798, PMCID: PMC5567998, DOI: 10.1038/nature22361.
- Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolutionRego EH, Shao L, Macklin JJ, Winoto L, Johansson GA, Kamps-Hughes N, Davidson MW, Gustafsson MG. Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 109: e135-e143. PMID: 22160683, PMCID: PMC3271870, DOI: 10.1073/pnas.1107547108.
- Super-resolution 3D microscopy of live whole cells using structured illuminationShao L, Kner P, Rego EH, Gustafsson MG. Super-resolution 3D microscopy of live whole cells using structured illumination. Nature Methods 2011, 8: 1044-1046. PMID: 22002026, DOI: 10.1038/nmeth.1734.
- Mycobacterial serine/threonine phosphatase PstP is phosphoregulated and localized to mediate control of cell wall metabolismShamma F, Rego E, Boutte C. Mycobacterial serine/threonine phosphatase PstP is phosphoregulated and localized to mediate control of cell wall metabolism. Molecular Microbiology 2022, 118: 47-60. PMID: 35670057, PMCID: PMC10070032, DOI: 10.1111/mmi.14951.
- Cell Wall Damage Reveals Spatial Flexibility in Peptidoglycan Synthesis and a Nonredundant Role for RodA in MycobacteriaMelzer E, Kado T, García-Heredia A, Gupta K, Meniche X, Morita Y, Sassetti C, Rego E, Siegrist M. Cell Wall Damage Reveals Spatial Flexibility in Peptidoglycan Synthesis and a Nonredundant Role for RodA in Mycobacteria. Journal Of Bacteriology 2022, 204: e00540-21. PMID: 35543537, PMCID: PMC9210966, DOI: 10.1128/jb.00540-21.
- Fighting microbial pathogens by integrating host ecosystem interactions and evolutionBurmeister AR, Hansen E, Cunningham JJ, Rego EH, Turner PE, Weitz JS, Hochberg ME. Fighting microbial pathogens by integrating host ecosystem interactions and evolution. BioEssays 2020, 43: e2000272. PMID: 33377530, PMCID: PMC9127240, DOI: 10.1002/bies.202000272.
- The conserved translation factor LepA is required for optimal synthesis of a porin family in Mycobacterium smegmatis.Fishbein S, Tomasi F, Wolf I, Dulberger C, Wang A, Keshishian H, Wallace L, Carr S, Ioerger T, Rego E, Rubin E. The conserved translation factor LepA is required for optimal synthesis of a porin family in Mycobacterium smegmatis. Journal Of Bacteriology 2020, 203 PMID: 33361193, PMCID: PMC8095456, DOI: 10.1128/jb.00604-20.
- Drug Susceptibility of Individual Mycobacterial CellsBoot M, Rego E. Drug Susceptibility of Individual Mycobacterial Cells. 2019, 247-272. DOI: 10.1007/978-3-030-25241-0_11.
- The Dream of a MycobacteriumBaranowski C, Rego E, Rubin E. The Dream of a Mycobacterium. 2019, 1096-1106. DOI: 10.1128/9781683670131.ch66.
- Rifampicin can induce antibiotic tolerance in mycobacteria via paradoxical changes in rpoB transcriptionZhu JH, Wang BW, Pan M, Zeng YN, Rego H, Javid B. Rifampicin can induce antibiotic tolerance in mycobacteria via paradoxical changes in rpoB transcription. Nature Communications 2018, 9: 4218. PMID: 30310059, PMCID: PMC6181997, DOI: 10.1038/s41467-018-06667-3.
- Characterization of Conserved and Novel Septal Factors in Mycobacterium smegmatisWu K, Zhang J, Baranowski C, Leung V, Rego E, Morita Y, Rubin E, Boutte C. Characterization of Conserved and Novel Septal Factors in Mycobacterium smegmatis. Journal Of Bacteriology 2018, 200: e00649-17. PMID: 29311277, PMCID: PMC5826036, DOI: 10.1128/jb.00649-17.
- Phosphorylation of the Peptidoglycan Synthase PonA1 Governs the Rate of Polar Elongation in MycobacteriaKieser K, Boutte C, Kester J, Baer C, Barczak A, Meniche X, Chao M, Rego E, Sassetti C, Fortune S, Rubin E. Phosphorylation of the Peptidoglycan Synthase PonA1 Governs the Rate of Polar Elongation in Mycobacteria. PLOS Pathogens 2015, 11: e1005010. PMID: 26114871, PMCID: PMC4483258, DOI: 10.1371/journal.ppat.1005010.
- Practical Structured Illumination MicroscopyRego EH, Shao L. Practical Structured Illumination Microscopy. 2014, 1251: 175-192. PMID: 25391800, DOI: 10.1007/978-1-4939-2080-8_10.
- Quantitative analysis and modeling of katanin function in flagellar length controlKannegaard E, Rego E, Schuck S, Feldman J, Marshall W. Quantitative analysis and modeling of katanin function in flagellar length control. Molecular Biology Of The Cell 2014, 25: 3686-3698. PMID: 25143397, PMCID: PMC4230626, DOI: 10.1091/mbc.e14-06-1116.
- Chapter 15 Structured Illumination MicroscopyShao L, Rego E. Chapter 15 Structured Illumination Microscopy. 2014, 213-225. DOI: 10.1016/b978-0-12-409513-7.00015-4.
- Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illuminationFiolka R, Shao L, Rego EH, Davidson MW, Gustafsson MG. Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 5311-5315. PMID: 22431626, PMCID: PMC3325651, DOI: 10.1073/pnas.1119262109.
- PALM and STORM: Unlocking live‐cell super‐resolutionHenriques R, Griffiths C, Rego E, Mhlanga M. PALM and STORM: Unlocking live‐cell super‐resolution. Biopolymers 2011, 95: 322-331. PMID: 21254001, DOI: 10.1002/bip.21586.