David Stitelman, MD
Associate Professor of Surgery (Pediatrics)
Research & Publications
Biography
News
Research Summary
The level of sophistication of prenatal imaging and genetic diagnostics is growing rapidly, however cures for many congenital anomalies lag behind our diagnostic abilities. In developmental defects where babies are born with a disease phenotype, fetal therapy offers an opportunity to prevent or cure these disease. This is an opportunity that is lost after birth.
There are 3 broad aims of my basic science laboratory: 1) In Utero gene editing for the treatment of genetic diseases, 2) In Utero reversal of lung hypoplasia in diaphragmatic hernia 3) Minimally invasive In Utero therapy for spina bifida.
Coauthors
Research Image
Particles Binding Fetal Spina Bifida
Green particles were injected into the amniotic space and can be appreciated binding to the exposed spinal cord in a pre-clinical rat model of spina bifida.
Selected Publications
- In utero delivery of miRNA induces epigenetic alterations and corrects pulmonary pathology in congenital diaphragmatic herniaUllrich S, Yung N, Bauer-Pisani T, Maassel N, Guerra M, Freedman-Weiss M, Ahle S, Ricciardi A, Sauler M, Saltzman W, Piotrowski-Daspit A, Stitelman D. In utero delivery of miRNA induces epigenetic alterations and corrects pulmonary pathology in congenital diaphragmatic hernia. Molecular Therapy - Nucleic Acids 2023, 32: 594-602. PMID: 37200861, PMCID: PMC10185702, DOI: 10.1016/j.omtn.2023.04.018.
- Cholecystitis and Gallstone DiseaseMaassel N, Stitelman D. Cholecystitis and Gallstone Disease. 2022, 837-841. DOI: 10.1007/978-3-031-07524-7_90.
- The parental journey of fetal care: a systematic review and metasynthesisWilpers A, Bahtiyar MO, Stitelman D, Batten J, Calix RX, Chase V, Yung N, Maassel N, Novick G. The parental journey of fetal care: a systematic review and metasynthesis. American Journal Of Obstetrics & Gynecology MFM 2021, 3: 100320. PMID: 33493706, DOI: 10.1016/j.ajogmf.2021.100320.
- Nanoparticles for delivery of agents to fetal lungsUllrich SJ, Freedman-Weiss M, Ahle S, Mandl HK, Piotrowski-Daspit AS, Roberts K, Yung N, Maassel N, Bauer-Pisani T, Ricciardi AS, Egan ME, Glazer PM, Saltzman WM, Stitelman DH. Nanoparticles for delivery of agents to fetal lungs. Acta Biomaterialia 2021, 123: 346-353. PMID: 33484911, PMCID: PMC7962939, DOI: 10.1016/j.actbio.2021.01.024.
- Anomalies of the Gastrointestinal TractRubinstein J, Farrelly J, Stitelman D, Christison-Lagay E. Anomalies of the Gastrointestinal Tract. 2020, 179-204. DOI: 10.1007/978-3-030-55660-0_9.
- Minimally Invasive Fetal Therapy for MyelomeningoceleFreedman-Weiss M, Stitelman D. Minimally Invasive Fetal Therapy for Myelomeningocele. Current Stem Cell Reports 2020, 6: 1-5. DOI: 10.1007/s40778-020-00167-1.
- An Evidence-Based Guideline Supporting Restricted Opioid Prescription after Pediatric AppendectomyFreedman-Weiss MR, Chiu AS, Worhunsky D, Manchisi A, Torres-Maldonado I, Sagnella L, Caty MG, Cowles RA, Ozgediz DE, Christison-Lagay ER, Solomon DG, Stitelman DH. An Evidence-Based Guideline Supporting Restricted Opioid Prescription after Pediatric Appendectomy. Journal Of Pediatric Surgery 2019, 55: 106-111. PMID: 31699433, DOI: 10.1016/j.jpedsurg.2019.09.063.
- Utility of Fetal Cardiac Axis and Cardiac Position Assessment in Predicting Neonatal Respiratory Morbidity in Fetal Congenital Lung LesionsTuzovic L, Copel JA, Stitelman DH, Levit O, Bahtiyar MO. Utility of Fetal Cardiac Axis and Cardiac Position Assessment in Predicting Neonatal Respiratory Morbidity in Fetal Congenital Lung Lesions. Journal Of Ultrasound In Medicine 2019, 38: 2361-2372. PMID: 30653685, DOI: 10.1002/jum.14931.
- Opioid Prescribing Habits of General Versus Pediatric Surgeons After Uncomplicated Laparoscopic AppendectomyFreedman-Weiss MR, Chiu A, Solomon DG, Christison-Lagay ER, Ozgediz DE, Cowles RA, Caty MG, Stitelman DH. Opioid Prescribing Habits of General Versus Pediatric Surgeons After Uncomplicated Laparoscopic Appendectomy. Journal Of Surgical Research 2018, 235: 404-409. PMID: 30691822, DOI: 10.1016/j.jss.2018.09.085.
- Routine postnatal chest x-ray and intensive care admission are unnecessary for a majority of infants with congenital lung malformationsGreig CJ, Keiser AM, Cleary MA, Stitelman DH, Christison-Lagay ER, Ozgediz DE, Solomon DG, Caty MG, Cowles RA. Routine postnatal chest x-ray and intensive care admission are unnecessary for a majority of infants with congenital lung malformations. Journal Of Pediatric Surgery 2018, 54: 670-674. PMID: 30503193, DOI: 10.1016/j.jpedsurg.2018.10.063.
- Alginate microparticles loaded with basic fibroblast growth factor induce tissue coverage in a rat model of myelomeningoceleFarrelly JS, Bianchi AH, Ricciardi AS, Buzzelli GL, Ahle SL, Freedman-Weiss MR, Luks VL, Saltzman WM, Stitelman DH. Alginate microparticles loaded with basic fibroblast growth factor induce tissue coverage in a rat model of myelomeningocele. Journal Of Pediatric Surgery 2018, 54: 80-85. PMID: 30414695, DOI: 10.1016/j.jpedsurg.2018.10.031.
- In utero nanoparticle delivery for site-specific genome editingRicciardi AS, Bahal R, Farrelly JS, Quijano E, Bianchi AH, Luks VL, Putman R, López-Giráldez F, Coşkun S, Song E, Liu Y, Hsieh WC, Ly DH, Stitelman DH, Glazer PM, Saltzman WM. In utero nanoparticle delivery for site-specific genome editing. Nature Communications 2018, 9: 2481. PMID: 29946143, PMCID: PMC6018676, DOI: 10.1038/s41467-018-04894-2.
- An atypical case of megacystis microcolon intestinal hypoperistalsis syndrome with extended survival and consistent bowel functionFarrelly J, Weiss R, Copel J, Porto A, Ahle S, Luks V, McGrath J, Stitelman D. An atypical case of megacystis microcolon intestinal hypoperistalsis syndrome with extended survival and consistent bowel function. Journal Of Pediatric Surgery Case Reports 2018, 30: 48-51. DOI: 10.1016/j.epsc.2017.11.010.
- Repair of a mycotic abdominal aortic aneurysm in a neonate using an everted jugular vein patchAhle SL, Cardella JA, Cleary MA, Stitelman DH, Sarac TP, Caty MG. Repair of a mycotic abdominal aortic aneurysm in a neonate using an everted jugular vein patch. Journal Of Vascular Surgery Cases And Innovative Techniques 2017, 3: 218-220. PMID: 29349429, PMCID: PMC5765176, DOI: 10.1016/j.jvscit.2017.08.003.
- Surgical tray optimization as a simple means to decrease perioperative costsFarrelly JS, Clemons C, Witkins S, Hall W, Christison-Lagay ER, Ozgediz DE, Cowles RA, Stitelman DH, Caty MG. Surgical tray optimization as a simple means to decrease perioperative costs. Journal Of Surgical Research 2017, 220: 320-326. PMID: 29180198, DOI: 10.1016/j.jss.2017.06.029.
- Complications in pediatric enteral and vascular accessFarrelly JS, Stitelman DH. Complications in pediatric enteral and vascular access. Seminars In Pediatric Surgery 2016, 25: 371-379. PMID: 27989361, DOI: 10.1053/j.sempedsurg.2016.10.006.
- Acquired seminal vesicle cyst in a teenager with a urethral stricture of unknown etiologyFarrelly J, Cleary M, Weiss R, Morotti R, Stitelman D. Acquired seminal vesicle cyst in a teenager with a urethral stricture of unknown etiology. Journal Of Pediatric Surgery Case Reports 2016, 7: 8-12. DOI: 10.1016/j.epsc.2016.01.002.
- Life-Long Transgene Expression in Skeletal Muscle Without Transduction of Satellite Cells Following Embryonic Myogenic Progenitor Transduction by Lentivirus Administered in UteroStitelman DH, Brazelton TR, Endo M, Bora A, Traas J, Zoltick PW, Flake AW. Life-Long Transgene Expression in Skeletal Muscle Without Transduction of Satellite Cells Following Embryonic Myogenic Progenitor Transduction by Lentivirus Administered in Utero. Stem Cells And Development 2015, 24: 1878-1887. PMID: 25915576, PMCID: PMC4533031, DOI: 10.1089/scd.2014.0585.
- Successful non-operative management of a contained tracheal tear following iatrogenic endotracheal tube injuryLuks V, Moores C, Villalona G, Stitelman D, Caty M. Successful non-operative management of a contained tracheal tear following iatrogenic endotracheal tube injury. Journal Of Pediatric Surgery Case Reports 2014, 2: 524-526. DOI: 10.1016/j.epsc.2014.10.016.
- Developmental stage determines efficiency of gene transfer to muscle satellite cells by in utero delivery of adeno-associated virus vector serotype 2/9Stitelman DH, Brazelton T, Bora A, Traas J, Merianos D, Limberis M, Davey M, Flake AW. Developmental stage determines efficiency of gene transfer to muscle satellite cells by in utero delivery of adeno-associated virus vector serotype 2/9. Molecular Therapy — Methods & Clinical Development 2014, 1: 14040. PMID: 26015979, PMCID: PMC4362369, DOI: 10.1038/mtm.2014.40.
- Increased epithelial stem cell migration in vivo in response to stem cell factor-1Bermudez D, Herdrich B, Stitelman D, Radu A, Li H, Zoltick P, Mitchell M. Increased epithelial stem cell migration in vivo in response to stem cell factor-1. Journal Of The American College Of Surgeons 2010, 211: s93. DOI: 10.1016/j.jamcollsurg.2010.06.246.
- Robust In Vivo Transduction of Nervous System and Neural Stem Cells by Early Gestational Intra Amniotic Gene Transfer Using Lentiviral VectorStitelman DH, Endo M, Bora A, Muvarak N, Zoltick PW, Flake AW, Brazelton TR. Robust In Vivo Transduction of Nervous System and Neural Stem Cells by Early Gestational Intra Amniotic Gene Transfer Using Lentiviral Vector. Molecular Therapy 2010, 18: 1615-1623. PMID: 20571539, PMCID: PMC2956924, DOI: 10.1038/mt.2010.125.
- Restoration of Function Following in Utero Fetal MI Is Associated with Increased Cardiac Progenitor Cell RecruitmentAllukian M, Herdrich B, Bermudez D, Stitelman D, Chin B, Davey M, Gorman J, Gorman R, Liechty K. Restoration of Function Following in Utero Fetal MI Is Associated with Increased Cardiac Progenitor Cell Recruitment. Journal Of Surgical Research 2010, 158: 307. DOI: 10.1016/j.jss.2009.11.379.
- Efficient gene transfer to muscle satellite cells by in utero intravenous delivery of adeno-associated viral vectorStitelman D, Bora A, Jeremy T, Merianos D, Flake A, Brazelton T. Efficient gene transfer to muscle satellite cells by in utero intravenous delivery of adeno-associated viral vector. Journal Of The American College Of Surgeons 2009, 209: s124. DOI: 10.1016/j.jamcollsurg.2009.06.309.
- Gene transfer to progenitor cells of the central nervous system by early intraamniotic delivery of lentiviral vectorStitelman D, Masayuki E, Zoltick P, Brazelton T, Flake A. Gene transfer to progenitor cells of the central nervous system by early intraamniotic delivery of lentiviral vector. Journal Of The American College Of Surgeons 2008, 207: s103. DOI: 10.1016/j.jamcollsurg.2008.06.263.
- Mechanism of angiopoietin-1 mediated endothelial progenitor cell recruitment in wound healingKeswani S, Parvadia J, Stitelman D, Kozin E, Radu A, Alaee D, Zoltick P, Crombleholme T. Mechanism of angiopoietin-1 mediated endothelial progenitor cell recruitment in wound healing. Journal Of The American College Of Surgeons 2004, 199: 60. DOI: 10.1016/j.jamcollsurg.2004.05.124.
- On the Effect of Covalently Appended Quinolones on Termini of DNA DuplexesTuma J, Connors W, Stitelman D, Richert C. On the Effect of Covalently Appended Quinolones on Termini of DNA Duplexes. Journal Of The American Chemical Society 2002, 124: 4236-4246. PMID: 11960452, DOI: 10.1021/ja0125117.
- Egr-1 Mediates Extracellular Matrix-driven Transcription of Membrane Type 1 Matrix Metalloproteinase in Endothelium*Haas T, Stitelman D, Davis S, Apte S, Madri J. Egr-1 Mediates Extracellular Matrix-driven Transcription of Membrane Type 1 Matrix Metalloproteinase in Endothelium*. Journal Of Biological Chemistry 1999, 274: 22679-22685. PMID: 10428849, DOI: 10.1074/jbc.274.32.22679.