2024
Electrophysiology of fluoride channels in the yeasts Saccharomyces cerevisiae and Candida albicans
Rivetta A, Slayman C. Electrophysiology of fluoride channels in the yeasts Saccharomyces cerevisiae and Candida albicans. Methods In Enzymology 2024, 696: 3-24. PMID: 38658085, DOI: 10.1016/bs.mie.2024.01.005.Peer-Reviewed Original ResearchYeast Saccharomyces cerevisiaeOpportunistic human pathogenFree-living microorganismsSingle cellsPlasma membrane of single cellsYeast spheroplastsSaccharomyces cerevisiaeRegulation of moleculesCandida albicansHuman pathogensPlasma membraneCell wallExtracellular environmentMembrane of single cellsPatch-clamp techniqueOsmotic strengthYeastSpheroplastsCandidaFluoride channelsSmall cell volumeToxic moleculesCell membraneDiverse moleculesCell populations
2015
Yeast Fex1p Is a Constitutively Expressed Fluoride Channel with Functional Asymmetry of Its Two Homologous Domains*
Smith KD, Gordon PB, Rivetta A, Allen KE, Berbasova T, Slayman C, Strobel SA. Yeast Fex1p Is a Constitutively Expressed Fluoride Channel with Functional Asymmetry of Its Two Homologous Domains*. Journal Of Biological Chemistry 2015, 290: 19874-19887. PMID: 26055717, PMCID: PMC4528147, DOI: 10.1074/jbc.m115.651976.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCell MembraneConserved SequenceDrug Resistance, FungalEscherichia coliFluoridesGene ExpressionGene Expression Regulation, FungalGenome, FungalIon TransportMembrane ProteinsMolecular Sequence DataMutationPatch-Clamp TechniquesPhosphorylationPhylogenyProtein FoldingProtein MultimerizationProtein Structure, SecondaryProtein Structure, TertiaryRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence AlignmentStatic ElectricityConceptsC-terminal domainSaccharomyces cerevisiae functionsYeast plasma membraneN-terminal domainEffects of mutationsDuplicate proteinsYeast genomeCerevisiae functionsSequence conservationHelix domainLinker helixUbiquitous environmental toxinHomologous domainsImportant residuesFluoride channelsPlasma membraneParticular residuesBiological speciesSimilar mutationsIon channelsContinuous expressionProteinAntiparallel dimerMutationsResidues