2024
Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties.
Shin J, Park J, Lim J, Jeong J, Dinesh R, Maher S, Kim J, Park S, Hong J, Wysolmerski J, Choi J, Bothwell A. Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties. ELife 2024, 13 PMID: 39535280, PMCID: PMC11560131, DOI: 10.7554/elife.97279.Peer-Reviewed Original ResearchConceptsCancer cellsDickkopf-2Analysis of transcriptomeGeneration of cancer cellsPositive cancer cellsStem cell niche factorsColon cancer cellsPaneth cell differentiationHepatocyte nuclear factor 4 alphaLysozyme positive cellsChromatin accessibilityHNF4A proteinSingle-cell RNA sequencing analysisCell propertiesPaneth cell markersSequence analysisChromatin immunoprecipitationPromoter regionTranscription factorsTranscriptome analysisColon cancerColon cancer metastasisReduction of liver metastasisDownstream targetsCell differentiation
2018
DKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation
Xiao Q, Wu J, Wang WJ, Chen S, Zheng Y, Yu X, Meeth K, Sahraei M, Bothwell ALM, Chen L, Bosenberg M, Chen J, Sexl V, Sun L, Li L, Tang W, Wu D. DKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation. Nature Medicine 2018, 24: 262-270. PMID: 29431745, PMCID: PMC5840007, DOI: 10.1038/nm.4496.Peer-Reviewed Original ResearchMeSH KeywordsAdenomatous Polyposis Coli ProteinBeta CateninCD8-Positive T-LymphocytesCell Line, TumorColorectal NeoplasmsCytotoxicity, ImmunologicGene Expression Regulation, NeoplasticHumansIntercellular Signaling Peptides and ProteinsIntestinal NeoplasmsKiller Cells, NaturalLow Density Lipoprotein Receptor-Related Protein-5MelanomaProgrammed Cell Death 1 ReceptorPTEN PhosphohydrolaseSignal TransductionSTAT5 Transcription FactorTumor Escape
2013
Neuroprotection against neuroblastoma cell death induced by depletion of mitochondrial glutathione
Dukhande VV, Kawikova I, Bothwell AL, Lai JC. Neuroprotection against neuroblastoma cell death induced by depletion of mitochondrial glutathione. Apoptosis 2013, 18: 702-712. PMID: 23494481, PMCID: PMC3645366, DOI: 10.1007/s10495-013-0836-4.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisAzolesBcl-2-Associated X ProteinButhionine SulfoximineCell Line, TumorCell SurvivalCoculture TechniquesCytochromes cCytosolEthacrynic AcidGlioblastomaGlutathioneHumansIsoindolesMitochondriaNeuroblastomaNeuronsOrganoselenium CompoundsProto-Oncogene Proteins c-bcl-2Reactive Oxygen SpeciesConceptsSK-N-SH cellsMitochondrial glutathioneCell deathNeuroblastoma cell deathMitochondrial dysfunctionCellular reactive oxygen speciesMitochondrial glutathione poolPrevention of apoptosisMitochondrial glutathione depletionNeurodegenerative disordersOxidative stressGlutathione depletionReactive oxygen speciesGlutathione poolDistinct inhibitorsApoptotic deathGlutathione metabolismEthacrynic acidROS productionOxygen speciesBCL2 overexpressionGSH depletionApoptosisL-buthionineCells
2006
Manganese Treatment Modulates the Expression of Peroxisome Proliferator-activated Receptors in Astrocytoma and Neuroblastoma Cells
Isaac AO, Kawikova I, Bothwell AL, Daniels CK, Lai JC. Manganese Treatment Modulates the Expression of Peroxisome Proliferator-activated Receptors in Astrocytoma and Neuroblastoma Cells. Neurochemical Research 2006, 31: 1305-1316. PMID: 17053972, DOI: 10.1007/s11064-006-9173-0.Peer-Reviewed Original ResearchConceptsPeroxisome proliferator-activated receptorMn neurotoxicityPPAR isoformsExpression of PPARsNeuroblastoma cellsProliferator-activated receptorAnti-oxidant responseCell typesChronic exposureNeurotoxic mechanismsPeroxisome proliferatorPathophysiological importanceHuman astrocytomasNeural cellsNeurotoxicityManganese chloride treatmentProtein expressionCell proliferationAstrocytomasManganese treatmentReceptorsMolecular mechanismsTreatmentCytosolic fractionCells
2004
Interferon α but Not Interleukin 12 Activates STAT4 Signaling in Human Vascular Endothelial Cells*
Torpey N, Maher SE, Bothwell AL, Pober JS. Interferon α but Not Interleukin 12 Activates STAT4 Signaling in Human Vascular Endothelial Cells*. Journal Of Biological Chemistry 2004, 279: 26789-26796. PMID: 15087447, DOI: 10.1074/jbc.m401517200.Peer-Reviewed Original ResearchMeSH KeywordsCell Line, TumorCells, CulturedChemokine CCL2DNADNA, ComplementaryDNA-Binding ProteinsDose-Response Relationship, DrugEndothelium, VascularEnzyme-Linked Immunosorbent AssayFlow CytometryHumansImmunoblottingInflammationInterferon-alphaInterleukin-12Oligonucleotide Array Sequence AnalysisPhosphorylationPrecipitin TestsProtein Structure, TertiaryRecombinant ProteinsRepressor ProteinsRetroviridaeReverse Transcriptase Polymerase Chain ReactionRNARNA, MessengerSignal TransductionSTAT4 Transcription FactorSuppressor of Cytokine Signaling 3 ProteinSuppressor of Cytokine Signaling ProteinsTime FactorsTrans-ActivatorsTranscription FactorsUmbilical VeinsUp-RegulationConceptsHuman umbilical vein ECMonocyte chemoattractant protein-1Quantitative RT-PCRVascular endothelial cellsEndothelial cellsChemokine monocyte chemoattractant protein-1Chemoattractant protein-1Cultured human umbilical vein endothelial cellsPro-inflammatory behaviorIFNalpha responseHuman vascular endothelial cellsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsNeuroblastoma cell linesSuppressor of cytokineVein endothelial cellsEffector cellsInterleukin-12T cellsSTAT4 pathwayInterferon αIL12 receptorCytokinesU3A cellsRT-PCR