2018
Regulation of human T cell responses by dNP2-ctCTLA-4 inhibits human skin and microvessel graft rejection
Lim S, Kirkiles-Smith NC, Pober JS, Bothwell ALM, Choi JM. Regulation of human T cell responses by dNP2-ctCTLA-4 inhibits human skin and microvessel graft rejection. Biomaterials 2018, 183: 128-138. PMID: 30165256, PMCID: PMC6141312, DOI: 10.1016/j.biomaterials.2018.08.049.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell ProliferationCell-Penetrating PeptidesCTLA-4 AntigenCytokinesEndothelial CellsFemaleGraft RejectionHuman Umbilical Vein Endothelial CellsHumansLymphocyte ActivationMice, Inbred BALB CMice, KnockoutMice, SCIDMicrovesselsReceptors, ChemokineSkinSkin TransplantationT-LymphocytesConceptsT cell responsesHuman T cell responsesT cell infiltrationHuman T cellsT cellsCell responsesGraft rejectionCell infiltrationSCID/beige miceCell-permeable peptideBlood cytokine levelsT cell alloresponsesCD8 T cellsChemokine receptor expressionGranzyme B expressionAlloreactive T cellsSignificant side effectsDouble knockout miceHuman T cell activationBcl-2-transduced human umbilical vein endothelial cellsT cell activationHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsSystemic immunosuppressantsAllograft rejectionDKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation
Xiao Q, Wu J, Wang WJ, Chen S, Zheng Y, Yu X, Meeth K, Sahraei M, Bothwell ALM, Chen L, Bosenberg M, Chen J, Sexl V, Sun L, Li L, Tang W, Wu D. DKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation. Nature Medicine 2018, 24: 262-270. PMID: 29431745, PMCID: PMC5840007, DOI: 10.1038/nm.4496.Peer-Reviewed Original ResearchMeSH KeywordsAdenomatous Polyposis Coli ProteinBeta CateninCD8-Positive T-LymphocytesCell Line, TumorColorectal NeoplasmsCytotoxicity, ImmunologicGene Expression Regulation, NeoplasticHumansIntercellular Signaling Peptides and ProteinsIntestinal NeoplasmsKiller Cells, NaturalLow Density Lipoprotein Receptor-Related Protein-5MelanomaProgrammed Cell Death 1 ReceptorPTEN PhosphohydrolaseSignal TransductionSTAT5 Transcription FactorTumor Escape
2017
Stat6 Promotes Intestinal Tumorigenesis in a Mouse Model of Adenomatous Polyposis by Expansion of MDSCs and Inhibition of Cytotoxic CD8 Response
Jayakumar A, Bothwell ALM. Stat6 Promotes Intestinal Tumorigenesis in a Mouse Model of Adenomatous Polyposis by Expansion of MDSCs and Inhibition of Cytotoxic CD8 Response. Neoplasia 2017, 19: 595-605. PMID: 28654863, PMCID: PMC5487300, DOI: 10.1016/j.neo.2017.04.006.Peer-Reviewed Original ResearchMeSH KeywordsAdenomatous Polyposis ColiAnimalsBecaplerminBiomarkersCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell Transformation, NeoplasticCytotoxicity, ImmunologicDisease Models, AnimalDisease ProgressionGene DeletionGene ExpressionInterleukin-4Intestinal MucosaIntestine, SmallMiceMice, KnockoutMyeloid-Derived Suppressor CellsProgrammed Cell Death 1 ReceptorProto-Oncogene Proteins c-sisSTAT6 Transcription FactorConceptsIntestinal tumorigenesisIL-4-induced STAT6Tumor-promoting growth factorsAntitumor T-cell responsesHuman colorectal cancer tissuesMore CD8 cellsPD-1 expressionEpithelial cellsExpansion of MDSCsT cell responsesIL-4 expressionCell proliferationColorectal cancer tissuesPlatelet-derived growth factor-BBIntestinal tumor progressionIntestinal epithelial cellsGrowth factor-BBColon cancer cell linesCD8 responsesPolyp progressionStrong CD8Cancer cell linesCD4 cellsCD8 cellsImmunosuppressive mediators
2006
Natural killer T cells and CD8+ T cells are dispensable for T cell–dependent allergic airway inflammation
Das J, Eynott P, Jupp R, Bothwell A, Van Kaer L, Shi Y, Das G. Natural killer T cells and CD8+ T cells are dispensable for T cell–dependent allergic airway inflammation. Nature Medicine 2006, 12: 1345-1346. PMID: 17151684, DOI: 10.1038/nm1206-1345.Peer-Reviewed Original ResearchPivotal roles of CD8+ T cells restricted by MHC class I–like molecules in autoimmune diseases
Das G, Das J, Eynott P, Zhang Y, Bothwell AL, Van Kaer L, Shi Y. Pivotal roles of CD8+ T cells restricted by MHC class I–like molecules in autoimmune diseases. Journal Of Experimental Medicine 2006, 203: 2603-2611. PMID: 17088432, PMCID: PMC2118151, DOI: 10.1084/jem.20060936.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutoimmune DiseasesBeta 2-MicroglobulinCD8-Positive T-LymphocytesCell SurvivalFemaleH-2 AntigensHistocompatibility Antigen H-2DHistocompatibility Antigens Class IIInflammatory Bowel DiseasesIslets of LangerhansLymphoproliferative DisordersMaleMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicNuclear ProteinsTrans-ActivatorsConceptsInnate-like lymphocytesInflammatory bowel diseaseMHC class IT cellsAutoimmune diseasesClass IMajor histocompatibility complex class IaLike moleculesClass II moleculesAdoptive transferBowel diseaseAutoimmune disordersLymphoproliferative syndromeImmunoregulatory functionsAdaptive immunityCD8Class IaDiseaseMiceInsulitisAutoimmunityPivotal roleCellsLymphocytesSyndromeDecreased Numbers of Regulatory T Cells Suggest Impaired Immune Tolerance in Children with Tourette Syndrome: A Preliminary Study
Kawikova I, Leckman JF, Kronig H, Katsovich L, Bessen DE, Ghebremichael M, Bothwell AL. Decreased Numbers of Regulatory T Cells Suggest Impaired Immune Tolerance in Children with Tourette Syndrome: A Preliminary Study. Biological Psychiatry 2006, 61: 273-278. PMID: 16996487, DOI: 10.1016/j.biopsych.2006.06.012.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAge of OnsetAntigens, CDAntigens, Differentiation, T-LymphocyteAutoantigensBlood Cell CountCD4 Lymphocyte CountCD8-Positive T-LymphocytesChildData Interpretation, StatisticalFemaleFlow CytometryHumansImmune ToleranceInterleukin-2 Receptor alpha SubunitLectins, C-TypeLymphocyte CountMaleStreptococcal InfectionsTic DisordersT-LymphocytesTourette SyndromeConceptsT reg cellsRegulatory T cellsReg cellsTourette syndromeT cellsTS patientsFluorescence-activated cell sortingHealthy age-matched control childrenHealthy age-matched control subjectsT reg cell numbersBeta-hemolytic streptococcal infectionAge-matched control subjectsHost T-cell immunityPathogenesis of TSHemolytic streptococcal infectionT cell immunitySignificant decreaseAge-matched control childrenAutoimmune inflammationCell immunityStreptococcal infectionAutoreactive lymphocytesImmune toleranceAutoimmune responsePeripheral blood
2002
Porcine Endothelial Cells, Unlike Human Endothelial Cells, Can Be Killed by Human CTL Via Fas Ligand and Cannot Be Protected by Bcl-2
Zheng L, Ben LH, Pober JS, Bothwell AL. Porcine Endothelial Cells, Unlike Human Endothelial Cells, Can Be Killed by Human CTL Via Fas Ligand and Cannot Be Protected by Bcl-2. The Journal Of Immunology 2002, 169: 6850-6855. PMID: 12471117, DOI: 10.4049/jimmunol.169.12.6850.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, HeterophileApoptosisCD8-Positive T-LymphocytesCell LineCell Line, TransformedCoculture TechniquesCytotoxicity Tests, ImmunologicCytotoxicity, ImmunologicEndothelium, VascularEpitopes, T-LymphocyteFas Ligand ProteinFas ReceptorGenetic VectorsGranzymesHistocompatibility Antigens Class IHumansImmunity, InnateIsoantigensLigandsMembrane GlycoproteinsPerforinPore Forming Cytotoxic ProteinsProto-Oncogene Proteins c-bcl-2Serine EndopeptidasesSwineT-Lymphocytes, CytotoxicConceptsHuman endothelial cellsPorcine endothelial cellsEndothelial cellsBcl-2MHC class I restrictionClass I restrictionPorcine aortic endothelial cellsTarget cell recognitionAnti-Fas ligand AbAortic endothelial cellsHost CTLAcute rejectionCTL responsesDrug-induced apoptosisHuman CTLPorcine targetsTarget cell typeHuman xenotransplantationFas expressionFas ligandPorcine cellsCTLMajor effectorCell recognitionMajor target