2018
Regulation of human T cell responses by dNP2-ctCTLA-4 inhibits human skin and microvessel graft rejection
Lim S, Kirkiles-Smith NC, Pober JS, Bothwell ALM, Choi JM. Regulation of human T cell responses by dNP2-ctCTLA-4 inhibits human skin and microvessel graft rejection. Biomaterials 2018, 183: 128-138. PMID: 30165256, PMCID: PMC6141312, DOI: 10.1016/j.biomaterials.2018.08.049.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell ProliferationCell-Penetrating PeptidesCTLA-4 AntigenCytokinesEndothelial CellsFemaleGraft RejectionHuman Umbilical Vein Endothelial CellsHumansLymphocyte ActivationMice, Inbred BALB CMice, KnockoutMice, SCIDMicrovesselsReceptors, ChemokineSkinSkin TransplantationT-LymphocytesConceptsT cell responsesHuman T cell responsesT cell infiltrationHuman T cellsT cellsCell responsesGraft rejectionCell infiltrationSCID/beige miceCell-permeable peptideBlood cytokine levelsT cell alloresponsesCD8 T cellsChemokine receptor expressionGranzyme B expressionAlloreactive T cellsSignificant side effectsDouble knockout miceHuman T cell activationBcl-2-transduced human umbilical vein endothelial cellsT cell activationHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsSystemic immunosuppressantsAllograft rejection
2003
Engraftment of a vascularized human skin equivalent
Schechner JS, Crane SK, Wang F, Szeglin AM, Tellides G, Lorber MI, Bothwell AL, Pober JS. Engraftment of a vascularized human skin equivalent. The FASEB Journal 2003, 17: 2250-2256. PMID: 14656987, DOI: 10.1096/fj.03-0257com.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsHuman skin equivalentsAcellular dermis graftsSkin equivalentsBcl-2-transduced human umbilical vein endothelial cellsUmbilical vein endothelial cellsEndothelial cell transplantationVein endothelial cellsCultured human keratinocytesCell transplantationSurvival gene bcl-2Orthotopic implantationSuccessful transplantationDermis graftImmunodeficient miceClinical utilityGraft vascularizationImpaired angiogenesisGene Bcl-2Bcl-2-transduced cellsAcellular dermisEndothelial cellsClinical performanceGraftBcl-2Immunopathology of human T cell responses to skin, artery and endothelial cell grafts in the human peripheral blood lymphocyte/severe combined immunodeficient mouse
Pober JS, Bothwell AL, Lorber MI, McNiff JM, Schechner JS, Tellides G. Immunopathology of human T cell responses to skin, artery and endothelial cell grafts in the human peripheral blood lymphocyte/severe combined immunodeficient mouse. Seminars In Immunopathology 2003, 25: 167-180. PMID: 12955465, DOI: 10.1007/s00281-003-0135-1.Peer-Reviewed Original ResearchConceptsLymphocytes/Peripheral blood lymphocyte/Human T cell responsesEndothelial cellsT cell responsesHuman peripheral bloodPig endotheliumHuman endothelial cellsPeripheral bloodCell graftsImmunodeficiency miceImmunodeficient miceCell responsesEndothelial liningRejection responseHuman vesselsBlood vesselsHuman arteriesLimited experienceTransplantationArteryImmunological propertiesSkinMiceHuman skin
2002
Interferon-γ Augments CD95(APO-1/Fas) and Pro-Caspase-8 Expression and Sensitizes Human Vascular Endothelial Cells to CD95-Mediated Apoptosis
Li JH, Kluger MS, Madge LA, Zheng L, Bothwell AL, Pober JS. Interferon-γ Augments CD95(APO-1/Fas) and Pro-Caspase-8 Expression and Sensitizes Human Vascular Endothelial Cells to CD95-Mediated Apoptosis. American Journal Of Pathology 2002, 161: 1485-1495. PMID: 12368221, PMCID: PMC1867313, DOI: 10.1016/s0002-9440(10)64424-0.Peer-Reviewed Original ResearchAmino Acid Chloromethyl KetonesAntigens, CDApoptosisCaspase 8Caspase 9CaspasesCell DeathCells, CulturedEndothelium, VascularEnzyme InhibitorsEnzyme PrecursorsFas Ligand ProteinFas ReceptorGene Expression RegulationGenetic VectorsHumansInterferon-gammaMembrane GlycoproteinsMicrocirculationRetroviridaeReverse Transcriptase Polymerase Chain ReactionSkinTransfectionUmbilical Veins