2001
Membrane Properties Underlying Patterns of GABA-Dependent Action Potentials in Developing Mouse Hypothalamic Neurons
Wang Y, Gao X, van den Pol A. Membrane Properties Underlying Patterns of GABA-Dependent Action Potentials in Developing Mouse Hypothalamic Neurons. Journal Of Neurophysiology 2001, 86: 1252-1265. PMID: 11535674, DOI: 10.1152/jn.2001.86.3.1252.Peer-Reviewed Original ResearchConceptsHypothalamic neuronsAction potentialsMouse hypothalamusMultiple spikesSpike thresholdFrequency of GABAMediobasal hypothalamic neuronsProbability of GABAGlutamate receptor antagonistsConventional whole cellMouse hypothalamic neuronsMultiple action potentialsPostnatal day 2Intrinsic membrane propertiesMean reversal potentialIonotropic glutamate receptorsSimilar spike patternsPeaks of depolarizationGABA excitationMembrane potentialReceptor antagonistSpike patternsExcitatory activityGlutamate receptorsSynaptic transmissionGABA, Not Glutamate, a Primary Transmitter Driving Action Potentials in Developing Hypothalamic Neurons
Gao X, Van Den Pol A. GABA, Not Glutamate, a Primary Transmitter Driving Action Potentials in Developing Hypothalamic Neurons. Journal Of Neurophysiology 2001, 85: 425-434. PMID: 11152743, DOI: 10.1152/jn.2001.85.1.425.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsBicucullineCells, CulturedChloridesExcitatory Amino Acid AntagonistsGABA-A Receptor AntagonistsGamma-Aminobutyric AcidGlutamic AcidGramicidinHypothalamusImmunohistochemistryIn Vitro TechniquesMiceNeuronsNeurotransmitter AgentsPatch-Clamp TechniquesReceptors, GlutamateSpinal CordConceptsHypothalamic neuronsReceptor antagonist bicucullinePrimary inhibitory neurotransmitterMouse hypothalamic neuronsPrimary excitatory transmitterAntagonist bicucullineExcitatory transmitterGABA circuitsGlutamate transmissionInhibitory neurotransmitterMature brainBrain slicesSynaptic releaseNeuronal activitySpike activityNeuronal connectionsSpike frequencyExtracellular recordingsAction potentialsAntagonist 2Brain developmentHebbian mechanismsIntracellular ClNeuronsGABA
2000
GABA release from mouse axonal growth cones
Gao X, van den Pol A. GABA release from mouse axonal growth cones. The Journal Of Physiology 2000, 523: 629-637. PMID: 10718743, PMCID: PMC2269824, DOI: 10.1111/j.1469-7793.2000.t01-1-00629.x.Peer-Reviewed Original ResearchConceptsGABA releaseSynapse formationGrowth conesSpontaneous GABA releaseAxonal growth conesHypothalamic neuronsTransmitter phenotypeAxon terminalsNeurotransmitter receptorsTransgenic miceAction potentialsGABAProtein kinase C activityNeuronal membranesAxonsNeuronal developmentGreen fluorescent proteinKinase C activityNeuronsVesicular mechanismPKC activationFluorescent proteinC activityLow levelsRelease
1998
Glutamate Inhibits GABA Excitatory Activity in Developing Neurons
van den Pol A, Gao X, Patrylo P, Ghosh P, Obrietan K. Glutamate Inhibits GABA Excitatory Activity in Developing Neurons. Journal Of Neuroscience 1998, 18: 10749-10761. PMID: 9852609, PMCID: PMC6793361, DOI: 10.1523/jneurosci.18-24-10749.1998.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornCalciumCells, CulturedCerebral CortexEmbryo, MammalianExcitatory Postsynaptic PotentialsGABA AntagonistsGamma-Aminobutyric AcidGene ExpressionGlutamic AcidGramicidinHypothalamusIn Vitro TechniquesNeuronsPatch-Clamp TechniquesPresynaptic TerminalsPropionatesRatsRats, Sprague-DawleyReceptors, Metabotropic GlutamateSpinal CordConceptsGroup III mGluRsExcitatory actionGABA activityGroup II metabotropic glutamate receptorsFura-2 digital imagingSynapse formationBrain developmentInhibitory roleAgonist L-AP4Excitatory GABA actionsGlutamate-mediated inhibitionMetabotropic glutamate receptorsSpinal cord neuronsMajor inhibitory neurotransmitterWhole-cell recordingsSimilar inhibitory actionBrain GABAEarly brain developmentExcitatory roleGABA releaseL-AP4Excitatory transmitterGABA actionHypothalamic neuronsCord neuronsPresynaptic and Postsynaptic Actions and Modulation of Neuroendocrine Neurons by a New Hypothalamic Peptide, Hypocretin/Orexin
van den Pol A, Gao X, Obrietan K, Kilduff T, Belousov A. Presynaptic and Postsynaptic Actions and Modulation of Neuroendocrine Neurons by a New Hypothalamic Peptide, Hypocretin/Orexin. Journal Of Neuroscience 1998, 18: 7962-7971. PMID: 9742163, PMCID: PMC6793026, DOI: 10.1523/jneurosci.18-19-07962.1998.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsArcuate Nucleus of HypothalamusCadmiumCalciumCarrier ProteinsCells, CulturedDose-Response Relationship, DrugElectric StimulationEnzyme InhibitorsEvoked PotentialsFeeding BehaviorFluorescent DyesFura-2Gamma-Aminobutyric AcidGlutamic AcidIntracellular Signaling Peptides and ProteinsMembrane PotentialsMolecular Sequence DataNeuronsNeuropeptidesNeurosecretory SystemsNeurotransmitter AgentsOrexinsPatch-Clamp TechniquesPresynaptic TerminalsSignal TransductionTetrodotoxinThapsigarginConceptsLateral hypothalamusSynaptic activityNeuroendocrine neuronsWhole-cell patch-clamp recordingsNew hypothalamic peptidesLateral hypothalamic neuronsAmino acid transmittersHypothalamic regulatory systemPatch-clamp recordingsHypocretin/orexinPerifornical areaPostsynaptic actionsOrexigenic peptideHypocretin-1Hypothalamic circuitsHypocretin peptidesHypothalamic neuronsHypothalamic slicesArcuate nucleusHypocretin-2Postsynaptic receptorsAxonal inputsHypothalamic peptidesFeeding centerAxon terminalsGABA-Dependent Firing of Glutamate-Evoked Action Potentials at AMPA/Kainate Receptors in Developing Hypothalamic Neurons
Gao X, Chen G, van den Pol A. GABA-Dependent Firing of Glutamate-Evoked Action Potentials at AMPA/Kainate Receptors in Developing Hypothalamic Neurons. Journal Of Neurophysiology 1998, 79: 716-726. PMID: 9463435, DOI: 10.1152/jn.1998.79.2.716.Peer-Reviewed Original ResearchConceptsGamma-aminobutyric acidAction potentialsHypothalamic neuronsAMPA/kainate receptorsN-methyl-D-aspartate receptorsGlutamate-mediated excitationAdult mammalian CNSFire action potentialsMajor inhibitory roleExcitatory functionGlutamate responseGlutamate receptorsKainate receptorsMammalian CNSMaximal effectSlight temporal delayNeuronal circuitsInhibitory roleNeuronsReceptorsBroad time windowDepolarizationGlutamatePresent studySimilar resultsThe hypocretins: Hypothalamus-specific peptides with neuroexcitatory activity
de Lecea L, Kilduff TS, Peyron C, Gao X, Foye PE, Danielson PE, Fukuhara C, Battenberg E, Gautvik VT, Bartlett FS, Frankel WN, van den Pol AN, Bloom FE, Gautvik KM, Sutcliffe JG. The hypocretins: Hypothalamus-specific peptides with neuroexcitatory activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 322-327. PMID: 9419374, PMCID: PMC18213, DOI: 10.1073/pnas.95.1.322.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCarrier ProteinsChromosomesConsensus SequenceHomeostasisHypothalamus, PosteriorIntracellular Signaling Peptides and ProteinsMaleMiceMolecular Sequence DataNeuronsNeuropeptidesNeurotransmitter AgentsOrexinsProtein PrecursorsRatsRats, WistarSecretinSynaptic VesiclesConceptsGut hormone secretinLateral hypothalamic areaNeuronal cell bodiesLarge granular vesiclesPosterior hypothalamusHypothalamic areaHypothalamic neuronsHippocampal neuronsCell bodiesGranular vesiclesHcrt peptidesHormone secretinNeuronsHypocretinNeuroexcitatory activityPeptidesPutative precursorBrainstemThalamusHypothalamusImmunoreactivityCNSNeurotransmittersSecretinProtein products