2021
Single-Cell Tracking By Time Lapse Imaging Confirms Thrombopoietin Promotes Megakaryocytic-Erythroid Progenitor Self Renewal, but Does Not Instruct Lineage Commitment
Scanlon V, Kochugaeva M, Lawton B, Xavier-Ferrucio J, Kang E, Eskow N, Lu Y, Kwon N, Laumas A, Cenci M, Lawrence K, Barden K, Larsuel S, Reed F, Pena-Carmona G, Ubbelohde A, Lee J, Boobalan S, Oppong Y, Anderson R, Maynard C, Sahirul K, Lajeune C, Ivathraya V, Addy T, Sanchez P, Holbrook C, Van Ho A, Blau H, Levchenko A, Krause D. Single-Cell Tracking By Time Lapse Imaging Confirms Thrombopoietin Promotes Megakaryocytic-Erythroid Progenitor Self Renewal, but Does Not Instruct Lineage Commitment. Blood 2021, 138: 3270. DOI: 10.1182/blood-2021-154360.Peer-Reviewed Original ResearchAbsence of thrombopoietinTime-lapse imagingLineage commitmentSelf-renewal divisionsCell typesMK lineageSelf-RenewalLineage-restricted progenitor cellsSpecific progenitor populationsProgenitor self-renewalProgenitor cellsSingle-cell trackingSpecific cell typesColony typesE lineageLineage choiceProgenitor commitmentCellular processesDaughter cellsLineage potentialCultured megakaryocytesProgenitor populationsCell statesDivision rateMegakaryocytic maturation
2019
Low iron promotes megakaryocytic commitment of megakaryocytic-erythroid progenitors in humans and mice
Xavier-Ferrucio J, Scanlon V, Li X, Zhang PX, Lozovatsky L, Ayala-Lopez N, Tebaldi T, Halene S, Cao C, Fleming MD, Finberg KE, Krause DS. Low iron promotes megakaryocytic commitment of megakaryocytic-erythroid progenitors in humans and mice. Blood 2019, 134: 1547-1557. PMID: 31439541, PMCID: PMC6839952, DOI: 10.1182/blood.2019002039.Peer-Reviewed Original ResearchConceptsMK lineage commitmentExtracellular signal-regulated kinase (ERK) pathwaySignal-regulated kinase pathwayMegakaryocytic-erythroid progenitorsBone marrow transplantation assaysSignal transduction analysisIron-deficient conditionsGene expression analysisMegakaryocytic commitmentLineage commitmentTransferrin receptor 2MK lineageTmprss6-/- miceIron sensorExpression analysisKinase pathwayTransduction analysisTransplantation assaysErythroid progenitorsMarrow environmentHematopoietic cellsMessenger RNAPhospho-ERK1/2Systemic iron deficiencyLow iron