Featured Publications
Multiparameter analysis of timelapse imaging reveals kinetics of megakaryocytic erythroid progenitor clonal expansion and differentiation
Scanlon VM, Thompson EN, Lawton BR, Kochugaeva M, Ta K, Mayday MY, Xavier-Ferrucio J, Kang E, Eskow NM, Lu YC, Kwon N, Laumas A, Cenci M, Lawrence K, Barden K, Larsuel ST, Reed FE, Peña-Carmona G, Ubbelohde A, Lee JP, Boobalan S, Oppong Y, Anderson R, Maynard C, Sahirul K, Lajeune C, Ivathraya V, Addy T, Sanchez P, Holbrook C, Van Ho AT, Duncan JS, Blau HM, Levchenko A, Krause DS. Multiparameter analysis of timelapse imaging reveals kinetics of megakaryocytic erythroid progenitor clonal expansion and differentiation. Scientific Reports 2022, 12: 16218. PMID: 36171423, PMCID: PMC9519589, DOI: 10.1038/s41598-022-19013-x.Peer-Reviewed Original ResearchConceptsMegakaryocytic-erythroid progenitorsFate specificationLineage commitmentUnderstanding of hematopoiesisProgenitor cell biologyPrimary human hematopoietic progenitorsSingle-cell trackingSingle-cell assaysSingle-cell levelHuman hematopoietic progenitorsProgenitor cell dynamicsLineage specificationCell fateColony-forming unit assaysTimelapse imagingSitu fluorescence stainingCell biologyLineage tracingDivision rateCytokine thrombopoietinHematopoietic progenitorsProgenitorsFluorescence stainingCell dynamicsUnit assays
2021
Single-Cell Tracking By Time Lapse Imaging Confirms Thrombopoietin Promotes Megakaryocytic-Erythroid Progenitor Self Renewal, but Does Not Instruct Lineage Commitment
Scanlon V, Kochugaeva M, Lawton B, Xavier-Ferrucio J, Kang E, Eskow N, Lu Y, Kwon N, Laumas A, Cenci M, Lawrence K, Barden K, Larsuel S, Reed F, Pena-Carmona G, Ubbelohde A, Lee J, Boobalan S, Oppong Y, Anderson R, Maynard C, Sahirul K, Lajeune C, Ivathraya V, Addy T, Sanchez P, Holbrook C, Van Ho A, Blau H, Levchenko A, Krause D. Single-Cell Tracking By Time Lapse Imaging Confirms Thrombopoietin Promotes Megakaryocytic-Erythroid Progenitor Self Renewal, but Does Not Instruct Lineage Commitment. Blood 2021, 138: 3270. DOI: 10.1182/blood-2021-154360.Peer-Reviewed Original ResearchAbsence of thrombopoietinTime-lapse imagingLineage commitmentSelf-renewal divisionsCell typesMK lineageSelf-RenewalLineage-restricted progenitor cellsSpecific progenitor populationsProgenitor self-renewalProgenitor cellsSingle-cell trackingSpecific cell typesColony typesE lineageLineage choiceProgenitor commitmentCellular processesDaughter cellsLineage potentialCultured megakaryocytesProgenitor populationsCell statesDivision rateMegakaryocytic maturation