2013
Inducible mouse models illuminate parameters influencing epigenetic inheritance
Wan M, Gu H, Wang J, Huang H, Zhao J, Kaundal RK, Yu M, Kushwaha R, Chaiyachati BH, Deerhake E, Chi T. Inducible mouse models illuminate parameters influencing epigenetic inheritance. Development 2013, 140: 843-852. PMID: 23325759, PMCID: PMC3557779, DOI: 10.1242/dev.088229.Peer-Reviewed Original ResearchConceptsTransgenerational inheritanceEpigenetic perturbationsModification patternsChromatin modification patternsRepressive chromatin modificationsAberrant epigenetic modificationsTarget gene sequenceMitotic inheritanceChromatin modificationsEpigenetic inheritanceEpigenetic stateMetastable epiallelesEpigenetic modificationsTranscription factorsGene sequencesDNA sequencesEpigenetic programmingTarget genesCOL1A1 locusFetal epigenomeExtraordinary malleabilityPleiotropic effectsInducible mouse modelEpigenomeTransient manipulationBRG1‐mediated immune tolerance: facilitation of Treg activation and partial independence of chromatin remodelling
Chaiyachati BH, Jani A, Wan Y, Huang H, Flavell R, Chi T. BRG1‐mediated immune tolerance: facilitation of Treg activation and partial independence of chromatin remodelling. The EMBO Journal 2013, 32: 395-408. PMID: 23321680, PMCID: PMC3567501, DOI: 10.1038/emboj.2012.350.Peer-Reviewed Original ResearchConceptsTreg activationRegulatory T cellsSeverity of inflammationChemokine receptor genesT-cell lineageΑβ T cell lineagesCD4 cellsSuppress autoimmunityProinflammatory roleImmune toleranceFatal inflammationT cellsInflammatory cuesTregsInflammationBrg1 deletionReceptor geneChromatin-remodelling factor BRG1Activation levelsActivationCell lineagesTarget genesATPase activityPoint mutationsCellsA General Approach for Controlling Transcription and Probing Epigenetic Mechanisms: Application to the Cd4 Locus
Wan M, Kaundal R, Huang H, Zhao J, Yang X, Chaiyachati BH, Li S, Chi T. A General Approach for Controlling Transcription and Probing Epigenetic Mechanisms: Application to the Cd4 Locus. The Journal Of Immunology 2013, 190: 737-747. PMID: 23293358, PMCID: PMC3744393, DOI: 10.4049/jimmunol.1201278.Peer-Reviewed Original Research
2012
LoxP-FRT Trap (LOFT): a simple and flexible system for conventional and reversible gene targeting
Chaiyachati BH, Kaundal RK, Zhao J, Wu J, Flavell R, Chi T. LoxP-FRT Trap (LOFT): a simple and flexible system for conventional and reversible gene targeting. BMC Biology 2012, 10: 96. PMID: 23198860, PMCID: PMC3529186, DOI: 10.1186/1741-7007-10-96.Peer-Reviewed Original ResearchEssential Roles of the Chromatin Remodeling Factor Brg1 in Spermatogenesis in Mice1
Wang J, Gu H, Lin H, Chi T. Essential Roles of the Chromatin Remodeling Factor Brg1 in Spermatogenesis in Mice1. Biology Of Reproduction 2012, 86: 186, 1-10. PMID: 22495890, PMCID: PMC3386149, DOI: 10.1095/biolreprod.111.097097.Peer-Reviewed Original ResearchConceptsMeiotic recombinationSomatic cellsDNA repairMeiotic sex chromosome inactivationChromatin Remodeling Factor BRG1Sex chromosome inactivationChromatin-remodeling complexBAF chromatin-remodeling complexImpaired homologous recombinationEssential roleRole of BRG1Chromatin regulationChromatin structureFactor BRG1Male germlineMammalian spermatogenesisSpatiotemporal regulationChromosome inactivationCatalytic subunitHomologous recombinationMidpachytene stageBRG1Expression patternsGene expressionGerm cells
2009
Molecular basis of CD4 repression by the Swi/Snf‐like BAF chromatin remodeling complex
Wan M, Zhang J, Lai D, Jani A, Prestone‐Hurlburt P, Zhao L, Ramachandran A, Schnitzler GR, Chi T. Molecular basis of CD4 repression by the Swi/Snf‐like BAF chromatin remodeling complex. European Journal Of Immunology 2009, 39: 580-588. PMID: 19180471, PMCID: PMC2774848, DOI: 10.1002/eji.200838909.Peer-Reviewed Original ResearchConceptsCD4 repressionCD4 silencerDominant negative mutantBAF complexNegative mutantSWI/SNF-like BAF chromatinBRG1/BRM-associated factor (BAF) chromatinCD4 silencer functionLinker histone H1Factor (BAF) chromatinT cell developmentKey repressorBAF chromatinSilencer functionBAF57Histone H1ChromatinMolecular basisFlanking regionsRepressionTranslational frameCD4 transcriptionSilencerEarly thymocytesMutants
2008
A novel genetic strategy reveals unexpected roles of the Swi–Snf–like chromatin-remodeling BAF complex in thymocyte development
Jani A, Wan M, Zhang J, Cui K, Wu J, Preston-Hurlburt P, Khatri R, Zhao K, Chi T. A novel genetic strategy reveals unexpected roles of the Swi–Snf–like chromatin-remodeling BAF complex in thymocyte development. Journal Of Experimental Medicine 2008, 205: 2813-2825. PMID: 18955569, PMCID: PMC2585832, DOI: 10.1084/jem.20080938.Peer-Reviewed Original ResearchConceptsPoint mutantsUnexpected roleImportant gene functionsThymocyte developmentNovel genetic strategyPoint mutationsEarly thymocyte developmentMammalian geneticsChromatin templatesSWI-SNFBAF complexGene functionATPase subunitsDeletion mutantsFactor complexCD4 locusTarget genesGenetic strategiesCD4 activationMutantsNovel activityPhysical interactionDeletionBRGMutationsNucleoprotein structure of the CD4 locus: Implications for the mechanisms underlying CD4 regulation during T cell development
Yu M, Wan M, Zhang J, Wu J, Khatri R, Chi T. Nucleoprotein structure of the CD4 locus: Implications for the mechanisms underlying CD4 regulation during T cell development. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 3873-3878. PMID: 18322012, PMCID: PMC2268827, DOI: 10.1073/pnas.0800810105.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD4 AntigensChromatin ImmunoprecipitationEnhancer Elements, GeneticGene DeletionGene Expression RegulationHistonesLymphocyte SubsetsMiceMice, Inbred C57BLModels, BiologicalNucleoproteinsPromoter Regions, GeneticProtein BindingSilencer Elements, TranscriptionalT-LymphocytesTranscription FactorsConceptsT cell developmentCell developmentNucleoprotein structuresCD4 locusHeterochromatin-like structureOpen chromatin configurationStage-specific mannerSame regulatory elementsDP cellsRepression patternsTranscription activatorCD4 silencerP300 recruitmentChromatin configurationRegulatory elementsDN cellsMolecular basisMolecular mechanismsCD4 enhancerCD4 transcriptionMature T cellsCD4 regulationCD4 geneCD4 promoterSilencer
2004
A BAF-centred view of the immune system
Chi T. A BAF-centred view of the immune system. Nature Reviews Immunology 2004, 4: 965-977. PMID: 15573131, DOI: 10.1038/nri1501.Peer-Reviewed Original ResearchConceptsChromatin-remodelling complexBAF complexRepressive chromatin structureStructure of chromatinHistone-DNA contactsTranscription factor complexPost-translational modificationsTranscriptional start siteIFN-β geneTranscriptional machineryChromatin structureTranscriptional activatorTranscriptional repressorHuman genesResponsive genesExpression of CIITAStart siteDNA sequencesFactor complexHistone octamerNucleosomesOwn replicationGene expressionGenomic DNAIFN-β expression
2002
Reciprocal regulation of CD4/CD8 expression by SWI/SNF-like BAF complexes
Chi TH, Wan M, Zhao K, Taniuchi I, Chen L, Littman DR, Crabtree GR. Reciprocal regulation of CD4/CD8 expression by SWI/SNF-like BAF complexes. Nature 2002, 418: 195-199. PMID: 12110891, DOI: 10.1038/nature00876.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD4 AntigensCD8 AntigensChromatinCross-Linking ReagentsDNA HelicasesDNA-Binding ProteinsFlow CytometryGene Expression RegulationGene SilencingMacromolecular SubstancesMiceMice, TransgenicMutationNuclear ProteinsProtein BindingProtein Structure, TertiaryProtein SubunitsThymus GlandTranscription FactorsConceptsBAF complexCD4 silencerYeast SWI/SNF complexSWI/SNF-like BAF complexHigh mobility group domainSWI/SNF complexLineage-specific genesDNA-binding subunitLineage bifurcationChromatin remodellingHMG domainChromatin lociSNF complexDNA bendingReciprocal regulationGroup domainCD4/CD8 expressionCell killingThymic developmentSilencerMutationsComplexesExpressionBAF57BRG
1998
Defects in actin-cap formation in Vav-deficient mice implicate an actin requirement for lymphocyte signal transduction
Holsinger L, Graef I, Swat W, Chi T, Bautista D, Davidson L, Lewis R, Alt F, Crabtree G. Defects in actin-cap formation in Vav-deficient mice implicate an actin requirement for lymphocyte signal transduction. Current Biology 1998, 8: 563-573. PMID: 9601640, DOI: 10.1016/s0960-9822(98)70225-8.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCell Cycle ProteinsCytoskeletonDNA-Binding ProteinsHumansJurkat CellsMiceNFATC Transcription FactorsNuclear ProteinsProto-Oncogene ProteinsProto-Oncogene Proteins c-vavReceptor-CD3 Complex, Antigen, T-CellReceptors, Antigen, T-CellSignal TransductionT-LymphocytesTranscription FactorsTranscription, GeneticConceptsMitogen-activated protein kinaseCap formationActin polymerizationGuanine nucleotide exchange factorsJun N-terminal kinaseTranscription factor NF-ATc1Vav-deficient miceActin-dependent pathwayStress-activated kinasesGrowth regulatory signalsActin cap formationAntigen receptor signalingEgr-1 geneLymphocyte signal transductionN-terminal kinaseAntigen-receptor interactionActin cytoskeletonExchange factorTranscriptional inductionSignal transductionDependent transcriptionProtein kinaseGTPase RacRegulatory signalsNull mutation