2016
Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast
Laplante C, Huang F, Tebbs IR, Bewersdorf J, Pollard TD. Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: e5876-e5885. PMID: 27647921, PMCID: PMC5056082, DOI: 10.1073/pnas.1608252113.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActinsCytokinesisInterphaseMicroscopy, FluorescenceModels, MolecularPhenotypeSchizosaccharomycesSchizosaccharomyces pombe ProteinsConceptsFluorescence photoactivation localization microscopyContractile ringLive fission yeast cellsLive fission yeastFission yeast cellsCytokinetic contractile ringSuper-resolution fluorescence microscopyCytokinesis nodesPhotoactivation localization microscopyFission yeastFormin Cdc12pActin networkIQ motifPlasma membraneCytokinesisConstituent proteinsMyosin-II tailYeast cellsActin ringsActin strandsAssembly precursorsDiscrete structural unitsFluorescence microscopyMolecular organizationProtein
2014
Cytokinetic nodes in fission yeast arise from two distinct types of nodes that merge during interphase
Akamatsu M, Berro J, Pu KM, Tebbs IR, Pollard TD. Cytokinetic nodes in fission yeast arise from two distinct types of nodes that merge during interphase. Journal Of Cell Biology 2014, 204: 977-988. PMID: 24637325, PMCID: PMC3998791, DOI: 10.1083/jcb.201307174.Peer-Reviewed Original Research
2007
Assembly Mechanism of the Contractile Ring for Cytokinesis by Fission Yeast
Vavylonis D, Wu JQ, Hao S, O'Shaughnessy B, Pollard TD. Assembly Mechanism of the Contractile Ring for Cytokinesis by Fission Yeast. Science 2007, 319: 97-100. PMID: 18079366, DOI: 10.1126/science.1151086.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonCytokinesisMicroscopy, ConfocalMicroscopy, FluorescenceModels, BiologicalMonte Carlo MethodMovementMyosin Type IISchizosaccharomycesStochastic ProcessesConceptsContractile ringActin filamentsLive fission yeast cellsIndividual daughter cellsFission yeast cellsDynamic actin filamentsFission yeastMotor protein myosinCell equatorDaughter cellsYeast cellsAssembly mechanismProtein myosinFluorescence microscopyCytokinesisMyosinFilamentsTransient connectionsCellsYeastFungiMechanismMeshwork
2006
Control of the Assembly of ATP- and ADP-Actin by Formins and Profilin
Kovar DR, Harris ES, Mahaffy R, Higgs HN, Pollard TD. Control of the Assembly of ATP- and ADP-Actin by Formins and Profilin. Cell 2006, 124: 423-435. PMID: 16439214, DOI: 10.1016/j.cell.2005.11.038.Peer-Reviewed Original ResearchConceptsATP hydrolysisTotal internal reflection fluorescence microscopyActin filament polymerizationReflection fluorescence microscopyDiversity of functionsPresence of profilinMammalian forminFormin proteinsFormin mDia1Processive elongationFilament polymerizationForminsActin filamentsBiochemical activityBarbed endsProfilinFluorescence microscopyADP-actinAssembly stepsRecent studiesMDia1Common featureProcessivityElongationProtein
2003
Spatial and Temporal Pathway for Assembly and Constriction of the Contractile Ring in Fission Yeast Cytokinesis
Wu JQ, Kuhn JR, Kovar DR, Pollard TD. Spatial and Temporal Pathway for Assembly and Constriction of the Contractile Ring in Fission Yeast Cytokinesis. Developmental Cell 2003, 5: 723-734. PMID: 14602073, DOI: 10.1016/s1534-5807(03)00324-1.Peer-Reviewed Original Research