2022
Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction
Xing H, Huang Y, Kunkemoeller B, Dahl P, Muraleetharan O, Malvankar N, Murrell M, Kyriakides T. Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction. Scientific Reports 2022, 12: 22474. PMID: 36577792, PMCID: PMC9797577, DOI: 10.1038/s41598-022-26337-1.Peer-Reviewed Original ResearchConceptsCell-derived matricesCell stiffnessFamily verprolin-homologous protein 2Active Rac1Thrombospondin-2Homologous protein 2Less traction forceCytoskeleton organizationExtracellular matrix productionMajor cell populationF-actinCytoskeletal disorganizationRegulatory roleProtein 2Matrix productionCritical functionsECM productionArt microscopy techniquesNew functionsCell populationsSpindle-like shapeRac1Normal fibroblastsFibroblastsWound space
2014
Engineering Cellular Response Using Nanopatterned Bulk Metallic Glass
Padmanabhan J, Kinser ER, Stalter MA, Duncan-Lewis C, Balestrini JL, Sawyer AJ, Schroers J, Kyriakides TR. Engineering Cellular Response Using Nanopatterned Bulk Metallic Glass. ACS Nano 2014, 8: 4366-4375. PMID: 24724817, PMCID: PMC4046793, DOI: 10.1021/nn501874q.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiocompatible MaterialsBiomechanical PhenomenaCell AdhesionCell SurvivalCollagenCytoskeletonFibroblastsFibronectinsForeign-Body ReactionGlassGTP PhosphohydrolasesHuman Umbilical Vein Endothelial CellsHumansMetalsMiceMicroscopy, FluorescenceNanostructuresNanotechnologyNIH 3T3 CellsProtein EngineeringRhoA GTP-Binding ProteinConceptsBulk metallic glassCellular responsesFeature sizeMetallic glassesFocal adhesion densityCell traction forcesCytoskeletal remodelingFIB-SEM techniqueSubstrate stiffnessBiomechanical cuesThermoplastic formingMolecular pathwaysCell typesVersatile fabricationNondimensional analysisSubstrate nanotopographyCellular morphologyCell morphologyBiocompatible materialsCell functionForeign body responseNanoscale precisionCell sizeTissue repairFibroblasts
2000
Matricellular Proteins as Modulators of Cell–Matrix Interactions: Adhesive Defect in Thrombospondin 2-null Fibroblasts is a Consequence of Increased Levels of Matrix Metalloproteinase-2
Yang Z, Kyriakides T, Bornstein P. Matricellular Proteins as Modulators of Cell–Matrix Interactions: Adhesive Defect in Thrombospondin 2-null Fibroblasts is a Consequence of Increased Levels of Matrix Metalloproteinase-2. Molecular Biology Of The Cell 2000, 11: 3353-3364. PMID: 11029041, PMCID: PMC14997, DOI: 10.1091/mbc.11.10.3353.Peer-Reviewed Original ResearchConceptsCell-matrix interactionsAbnormal cell-matrix interactionsWild-type cellsTSP2-null miceAdhesive defectsComplex phenotypesCell spreadingMolecular mechanismsMutant fibroblastsProtein resultsMatrix proteinsStable transfectionMatrix metalloproteinasesMetalloproteinase-2Matricellular proteinAltered expressionTHBS2 geneNull miceAffinity chromatographyGelatinolytic assaysThrombospondin-2TSP2Matrix metalloproteinase-2FibroblastsLevels of MMP2