2025
High-throughput multiplex voltage-clamp/current-clamp evaluation of acutely isolated neurons
Ghovanloo M, Tyagi S, Zhao P, Kiziltug E, Estacion M, Effraim P, Dib-Hajj S, Waxman S. High-throughput multiplex voltage-clamp/current-clamp evaluation of acutely isolated neurons. Nature Protocols 2025, 1-26. PMID: 40514421, DOI: 10.1038/s41596-025-01194-0.Peer-Reviewed Original ResearchPatch-clamp techniquePatch-clamp methodPatch-clamp approachPatch-clamp experimentsHeterologous cell linesIsolated neuronsInvestigated receptorsGraphical user interfaceIsolation of neuronsCell preparationsExcitable cellsOpen-source softwareCell linesNeuronsFiltration criteriaIndividual neuronsUser interfaceNative stateDrug screeningRobotic systemDrug developmentManual implementationBiophysical equationsComplex datasetsIntact tissueIn vitro inhibition of voltage-dependent sodium currents by the antifungal drug amorolfine
Ghovanloo M, Tyagi S, Effraim P, Waxman S. In vitro inhibition of voltage-dependent sodium currents by the antifungal drug amorolfine. Journal Of Biological Chemistry 2025, 301: 108407. PMID: 40090585, PMCID: PMC12004706, DOI: 10.1016/j.jbc.2025.108407.Peer-Reviewed Original ResearchNav channelsInhibition of voltage-dependent sodium currentsLocal anestheticsVoltage-dependent sodium currentsStabilization of inactivationVoltage-gated sodiumSodium currentChannel inactivationChannel inhibitorsAmorolfineInactivated statePharmacological modulationIn vitro inhibitionIon channelsInhibitorsNav
2024
TRPM8 Mutations Associated With Persistent Pain After Surgical Injury of Corneal Trigeminal Axons
Ghovanloo M, Effraim P, Tyagi S, Aldrich A, Cheng X, Yuan J, Schulman B, Jacobs D, Dib-Hajj S, Waxman S. TRPM8 Mutations Associated With Persistent Pain After Surgical Injury of Corneal Trigeminal Axons. Neurology Genetics 2024, 10: e200206. PMID: 39555137, PMCID: PMC11567650, DOI: 10.1212/nxg.0000000000200206.Peer-Reviewed Original ResearchLaser-assisted in situ keratomileusisPostoperative ocular painTrigeminal ganglion neuronsOcular painMultielectrode array recordingsPersistent painGanglion neuronsLaser-assisted in situ keratomileusis surgeryAxonal injuryRat trigeminal ganglion neuronsTransient receptor potential cation channelCorneal refractive surgeryMultielectrode arraysAnalysis of patientsPatch-clamp analysisGenomic analysis of patientsWild-typePatch-clamp resultsExposure to mentholRefractive surgeryHyperpolarizing directionNeuronal hyperexcitabilityPain-freeTrigeminal axonsWT channelsImpacts of resident physician unionization on house staff compensation
Tyagi S, Shah R, Huttler J, Kayani J, Ghovanloo M, Effraim P. Impacts of resident physician unionization on house staff compensation. PLOS ONE 2024, 19: e0308100. PMID: 39361626, PMCID: PMC11449312, DOI: 10.1371/journal.pone.0308100.Peer-Reviewed Original ResearchConceptsNon-union counterpartsPhysician unionsWorking long hoursUnion statusResidency training programsUnion programCost-of-livingLow wagesStaff compensationLong hoursTraining programSalaryInternal medicine residency training programsPhysicians-in-trainingRegional factorsDisbursementBenefit dataCross-sectional investigationMonetary benefitsUnionResident salariesCompensationCost-of-living differencesPGY-1Residency programsTRPM8 mutations associated with persistent ocular pain after refractive surgery: D665N and V915M
Ghovanloo M, Effraim P, Tyagi S, Cheng X, Yuan J, Schulman B, Jacobs D, Dib-Hajj S, Waxman S. TRPM8 mutations associated with persistent ocular pain after refractive surgery: D665N and V915M. Biophysical Journal 2024, 123: 391a. DOI: 10.1016/j.bpj.2023.11.2376.Peer-Reviewed Original ResearchFunctionally-selective inhibition of threshold sodium currents and excitability in dorsal root ganglion neurons by cannabinol
Ghovanloo M, Effraim P, Tyagi S, Zhao P, Dib-Hajj S, Waxman S. Functionally-selective inhibition of threshold sodium currents and excitability in dorsal root ganglion neurons by cannabinol. Communications Biology 2024, 7: 120. PMID: 38263462, PMCID: PMC10805714, DOI: 10.1038/s42003-024-05781-x.Peer-Reviewed Original ResearchConceptsDorsal root ganglionDorsal root ganglion neuronal excitabilityDorsal root ganglion neuronsNeuronal excitabilityCurrent-clamp analysisSteady-state inactivationVoltage-dependent sodiumSlow inactivated stateAutomated patch clamp platformMultielectrode array recordingsNav currentsNeuropathic painSodium currentRoot ganglionGanglion neuronsSlow inactivationInactivated stateCurrent inhibitorsIon channelsNeuronsInhibitory effectCannabinolArray recordingsEndocannabinoidCannabinoid
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