2025
GIT ArfGAP2: A Cytoskeletal Safeguard in Podocytes
Ishibe S, Tian X. GIT ArfGAP2: A Cytoskeletal Safeguard in Podocytes. Journal Of The American Society Of Nephrology 2025, 36: 1005-1007. PMID: 40257840, PMCID: PMC12147969, DOI: 10.1681/asn.0000000707.Peer-Reviewed Original ResearchCRB2 depletion induces YAP signaling and disrupts mechanosensing in podocytes
Sun Y, Kronenberg N, Sethi S, Dash S, Kovalik M, Sempowski B, Strickland S, Raina R, Sperati C, Tian X, Ishibe S, Hall G, Gather M. CRB2 depletion induces YAP signaling and disrupts mechanosensing in podocytes. American Journal Of Physiology. Renal Physiology 2025, 328: f578-f595. PMID: 40062402, PMCID: PMC12076484, DOI: 10.1152/ajprenal.00318.2024.Peer-Reviewed Original ResearchConceptsSubstrate stiffness-dependent mannerYes-associated proteinElastic Resonator Interference Stress MicroscopyCrumbs homolog 2Increasing substrate stiffnessStiffness-dependent mannerSubstrate stiffnessKnockdown cellsDensity of focal adhesionsCompound heterozygous mutationsHomolog 2Truncating mutation p.Low substrate stiffnessYes-associated protein signalingHeterozygous mutationsYes-associated protein transcriptional activityTarget gene expressionEnhanced cell contractilityFocal adhesionsTruncated p.Knockdown effectF-actinTranscriptional activityYAP activityPathogenic mutations
2024
ZFYVE21 promotes endothelial nitric oxide signaling and vascular barrier function in the kidney during aging
Jiang Q, Song G, He L, Li X, Jiang B, Wang Q, Wang S, Kim C, Barkestani M, Lopez R, Fan M, Wanniarachchi K, Quaranta M, Tian X, Mani A, Gonzalez A, Goodwin J, Sessa W, Ishibe S, Jane-Wit D. ZFYVE21 promotes endothelial nitric oxide signaling and vascular barrier function in the kidney during aging. Kidney International 2024, 106: 419-432. PMID: 38797325, PMCID: PMC11343665, DOI: 10.1016/j.kint.2024.05.007.Peer-Reviewed Original ResearchVascular barrier functionEndothelial cellsReduced endothelial nitric oxideRegulator of vascular barrier functionEndothelial nitric oxide signalingENOS activityEndosome-associated proteinsBarrier functionKidney organ culturesEndothelial nitric oxideAkt-dependent mannerNitric oxide donorGTPase Rab5Nitric oxide signalingZFYVE21Live cell imagingKidney insufficiencyReporter miceTrafficking mechanismsAccelerated aging phenotypeKnockout miceInterstitial edemaKidney functionVesicular populationOxide donor
2023
Profilin1 is required to prevent mitotic catastrophe in murine and human glomerular diseases
Tian X, Pedigo C, Li K, Ma X, Bunda P, Pell J, Lek A, Gu J, Zhang Y, Rangel P, Li W, Schwartze E, Nagata S, Lerner G, Perincheri S, Priyadarshini A, Zhao H, Lek M, Menon M, Fu R, Ishibe S. Profilin1 is required to prevent mitotic catastrophe in murine and human glomerular diseases. Journal Of Clinical Investigation 2023, 133: e171237. PMID: 37847555, PMCID: PMC10721156, DOI: 10.1172/jci171237.Peer-Reviewed Original ResearchConceptsProteinuric kidney diseaseKidney diseasePodocyte lossHuman glomerular diseasesMitotic catastrophePodocyte cell cycleSevere proteinuriaCell cycle reentryKidney failureGlomerular diseaseCell cycleKidney tissueG1/S checkpointUnsuccessful repairCyclin D1Glomerular integrityIrregular nucleiTissue-specific lossMouse podocytesPodocytesAltered expressionDiseaseCyclin B1Ribosome affinity purificationMultinucleated cellsUncovering the Role of Cyclin G-Associated Kinase (GAK) in Regulating the Podocyte Cytoskeleton
Bunda P, Tian X, Inoue K, Ishibe S. Uncovering the Role of Cyclin G-Associated Kinase (GAK) in Regulating the Podocyte Cytoskeleton. Journal Of The American Society Of Nephrology 2023, 34: 302-303. DOI: 10.1681/asn.20233411s1302d.Peer-Reviewed Original Research
2022
Cell Cycle and Senescence Regulation by Podocyte Histone Deacetylase 1 and 2
Rangel P, Cross E, Liu C, Pedigo C, Tian X, Gutiérrez-Calabrés E, Nagata S, Priyadarshini A, Lerner G, Bunda P, Perincheri S, Gu J, Zhao H, Wang Y, Inoue K, Ishibe S. Cell Cycle and Senescence Regulation by Podocyte Histone Deacetylase 1 and 2. Journal Of The American Society Of Nephrology 2022, 34: 433-450. PMID: 36414418, PMCID: PMC10103311, DOI: 10.1681/asn.2022050598.Peer-Reviewed Original ResearchConceptsCell cycle entryDNA damageSenescence-associated β-galactosidase activityDouble knockout miceRole of HDACsNormal glomerular filtration barrierAssociated phenotypesP21-mediated cell cycle arrestOpen chromatin conformationGlomerular filtration barrierSevere proteinuriaKidney failureProinflammatory cytokinesCell cycle regulationHistone deacetylase 1Cell cycle arrestKi67 expressionSustained DNA damagePodocyte lossIntact expressionMice leadsPodocyte-specific lossMatrix metalloproteinasesPodocyte detachmentProteinuria
2015
Podocyte-associated talin1 is critical for glomerular filtration barrier maintenance
Tian X, Kim J, Monkley S, Gotoh N, Nandez R, Soda K, Inoue K, Balkin D, Hassan H, Son S, Lee Y, Moeckel G, Calderwood D, Holzman L, Critchley D, Zent R, Reiser J, Ishibe S. Podocyte-associated talin1 is critical for glomerular filtration barrier maintenance. Journal Of Clinical Investigation 2015, 125: 882-882. PMCID: PMC4319418, DOI: 10.1172/jci80814.Peer-Reviewed Original Research
2014
Podocyte-associated talin1 is critical for glomerular filtration barrier maintenance
Tian X, Kim JJ, Monkley SM, Gotoh N, Nandez R, Soda K, Inoue K, Balkin DM, Hassan H, Son SH, Lee Y, Moeckel G, Calderwood DA, Holzman LB, Critchley DR, Zent R, Reiser J, Ishibe S. Podocyte-associated talin1 is critical for glomerular filtration barrier maintenance. Journal Of Clinical Investigation 2014, 124: 1098-1113. PMID: 24531545, PMCID: PMC3934159, DOI: 10.1172/jci69778.Peer-Reviewed Original ResearchConceptsNephrotic syndromeFoot process effacementLoss of talin1Glomerular filtration barrierGlomerular injuryMurine modelProcess effacementKidney's glomerular filtration barrierFiltration barrierGlomerular basement membraneSevere proteinuriaKidney failurePharmacologic inhibitionSyndromeBarrier maintenanceCalpain activityIntegrin activationEpithelial cellsPodocytesModest reductionÎ’1 integrin activationBasement membranePathogenesisInjuryCytoskeletal protein talin1
2012
Role of dynamin, synaptojanin, and endophilin in podocyte foot processes
Soda K, Balkin DM, Ferguson SM, Paradise S, Milosevic I, Giovedi S, Volpicelli-Daley L, Tian X, Wu Y, Ma H, Son SH, Zheng R, Moeckel G, Cremona O, Holzman LB, De Camilli P, Ishibe S. Role of dynamin, synaptojanin, and endophilin in podocyte foot processes. Journal Of Clinical Investigation 2012, 122: 4401-4411. PMID: 23187129, PMCID: PMC3533561, DOI: 10.1172/jci65289.Peer-Reviewed Original ResearchConceptsRole of dynaminNormal embryonic developmentFiltration barrierSynaptic vesicle recyclingFoot process formationKidney filtration barrierGlomerular filtration barrierNeuronal synapse developmentDynamin's roleEndophilin 3Actin cytoskeletonActin dynamicsFunctional partnersDynamin 1Endophilin-1Embryonic developmentVesicle recyclingProtein networkKidney's glomerular filtration barrierSynapse developmentDynaminPodocyte foot processesNeuronal synapsesSynaptojaninEndophilin
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