2017
Pioneer Factors FOXA1 and FOXA2 Assist Selective Glucocorticoid Receptor Signaling in Human Endometrial Cells
Whirledge S, Kisanga EP, Taylor RN, Cidlowski JA. Pioneer Factors FOXA1 and FOXA2 Assist Selective Glucocorticoid Receptor Signaling in Human Endometrial Cells. Endocrinology 2017, 158: 4076-4092. PMID: 28938408, PMCID: PMC5695839, DOI: 10.1210/en.2017-00361.Peer-Reviewed Original ResearchConceptsPioneer factor FOXA1Polymerase 2Molecular mechanismsRecruitment of GRThousands of genesExpression of lefty1GR-dependent inductionGene expression analysisHuman endometrial cellsGlucocorticoid receptorGlucocorticoid-mediated inductionEndometrial cellsRegulatory regionsGlucocorticoid receptor signalingMaster regulatorSpecific genesExpression analysisPrimary human endometrial cellsGene expressionHuman uterine cellsGlucocorticoid-dependent inductionIntracellular signalingEmbryo implantationFOXA2Receptor recruitmentGenerating diversity in human glucocorticoid signaling through a racially diverse polymorphism in the beta isoform of the glucocorticoid receptor
Whirledge SD, Jewell CM, Barber LM, Xu X, Katen KS, Garantziotis S, Cidlowski JA. Generating diversity in human glucocorticoid signaling through a racially diverse polymorphism in the beta isoform of the glucocorticoid receptor. Laboratory Investigation 2017, 97: 1282-1295. PMID: 28759007, PMCID: PMC5759773, DOI: 10.1038/labinvest.2017.76.Peer-Reviewed Original ResearchMeSH Keywords3' Untranslated RegionsAdultAmino Acid SubstitutionBlack PeopleCell Line, TumorCells, CulturedCohort StudiesFemaleGene Expression RegulationGenetic Association StudiesGlucocorticoidsHispanic or LatinoHumansMacrophagesMaleNorth CarolinaPolymorphism, Single NucleotideProspective StudiesReceptors, GlucocorticoidRecombinant ProteinsRegistriesSignal TransductionWhite PeopleConceptsGene expression profilesGene expressionExpression profilesGlucocorticoid sensitivityT polymorphismWide gene expressionPrimary macrophagesInherent transcriptional activityHigher serum cortisol levelsLow-dose dexamethasoneProspective cohort studyProtein expressionSelf-reported allergiesDominant negative regulatorSerum cortisol levelsReceptor protein expressionSelf-reported diseasesGlucocorticoid receptor geneHuman glucocorticoid receptor geneMinor allele frequencyNon-Hispanic participantsAlternative splicingMRNA stabilityReceptor stabilityTranscriptional activityGlucocorticoids and Reproduction: Traffic Control on the Road to Reproduction
Whirledge S, Cidlowski JA. Glucocorticoids and Reproduction: Traffic Control on the Road to Reproduction. Trends In Endocrinology And Metabolism 2017, 28: 399-415. PMID: 28274682, PMCID: PMC5438761, DOI: 10.1016/j.tem.2017.02.005.Peer-Reviewed Original ResearchConceptsDiverse cellular functionsStress-induced levelsCell-specific mannerCellular functionsGlucocorticoid receptorCellular processingGR geneReproductive functionNormal physiologyActivity of glucocorticoidsCompelling new evidenceReproductionReproductive dysfunctionGlucocorticoid responsivenessGlucocorticoidsSteroid hormonesGenesProteinDiversityPhysiologyRegulationFunctionReceptorsNew evidenceMaintenance
2013
A Role for Glucocorticoids in Stress-Impaired Reproduction: Beyond the Hypothalamus and Pituitary
Whirledge S, Cidlowski JA. A Role for Glucocorticoids in Stress-Impaired Reproduction: Beyond the Hypothalamus and Pituitary. Endocrinology 2013, 154: 4450-4468. PMID: 24064362, PMCID: PMC3836069, DOI: 10.1210/en.2013-1652.Peer-Reviewed Original ResearchMeSH KeywordsFemaleGlucocorticoidsHumansHypothalamusInfertility, FemalePituitary GlandStress, PhysiologicalConceptsGlucocorticoid signalingAbsence of stressOffspring fitnessReproductive investmentReproductive successGonadal axisCentral regulatorEvolutionary advantageStress responseReproductive eventsSex steroid receptorsAction of glucocorticoidsReproductive statusReproductionReproductive systemCentral actionAdrenal axisSignalingGlucocorticoid secretionImmune responseGlucocorticoid receptorStress contributesSteroid receptorsGlucocorticoidsMetabolic statusGlobal Gene Expression Analysis in Human Uterine Epithelial Cells Defines New Targets of Glucocorticoid and Estradiol Antagonism1
Whirledge S, Xu X, Cidlowski JA. Global Gene Expression Analysis in Human Uterine Epithelial Cells Defines New Targets of Glucocorticoid and Estradiol Antagonism1. Biology Of Reproduction 2013, 89: 66, 1-17. PMID: 23843231, PMCID: PMC4435104, DOI: 10.1095/biolreprod.113.111054.Peer-Reviewed Original ResearchConceptsImmune systemUterine endometrial cancer cell lineUterine epitheliumEndometrial cancer cell linesHuman uterine epithelial cellsMaintenance of pregnancyEffects of glucocorticoidsImmune cell traffickingSynthetic glucocorticoid dexamethasoneCell linesEpithelial-like cell lineFemale reproductive systemPresence of DexGlucocorticoid-regulated genesIngenuity Pathway AnalysisUterine epithelial cellsCancer cell linesInflammatory responseReproductive hormonesEstrogen receptorGlucocorticoid signalingEmbryo implantationGlucocorticoid dexamethasoneGlucocorticoid receptorGlucocorticoids
2012
Estradiol Antagonism of Glucocorticoid-Induced GILZ Expression in Human Uterine Epithelial Cells and Murine Uterus
Whirledge S, Cidlowski JA. Estradiol Antagonism of Glucocorticoid-Induced GILZ Expression in Human Uterine Epithelial Cells and Murine Uterus. Endocrinology 2012, 154: 499-510. PMID: 23183181, PMCID: PMC3529382, DOI: 10.1210/en.2012-1748.Peer-Reviewed Original ResearchConceptsGILZ gene expressionGILZ expressionEstrogen receptorGlucocorticoid receptorInterplay of estrogensSex hormone signalingEpithelial cellsER antagonist ICIImmune modulating functionsHuman uterine epithelial cellsCombination of dexamethasoneUterine epithelial cellsDexamethasone decreasesImmune-related functionsGlucocorticoid response elementMurine uterusGene expressionMouse uterusGlucocorticoid signalingEmbryo implantationUterine endometriumGILZ promoterGlucocorticoidsHormone treatmentReceptorsGlucocorticoids Regulate Gene Expression and Repress Cellular Proliferation in Human Uterine Leiomyoma Cells
Whirledge S, Dixon D, Cidlowski JA. Glucocorticoids Regulate Gene Expression and Repress Cellular Proliferation in Human Uterine Leiomyoma Cells. Discover Oncology 2012, 3: 79-92. PMID: 22311344, PMCID: PMC4772901, DOI: 10.1007/s12672-012-0103-0.Peer-Reviewed Original ResearchConceptsUterine leiomyoma cellsHuman uterine leiomyoma cellsLeiomyoma cellsGlucocorticoid receptorUterine leiomyomaUterine leiomyoma treatmentAbsence of estrogenPotential roleSmooth muscle cellsSynthetic glucocorticoid dexamethasoneIngenuity Pathway AnalysisLeiomyoma treatmentSignificant glucocorticoidE2 effectsEstrogen treatmentCell growthSex hormonesGenome-wide microarray studiesGlucocorticoid dexamethasoneGlucocorticoidsS-phase cellsNormal myometriumFlow cytometryMuscle cellsLeiomyoma
2010
Glucocorticoids, stress, and fertility.
Whirledge S, Cidlowski JA. Glucocorticoids, stress, and fertility. Minerva Endocrinologica 2010, 35: 109-25. PMID: 20595939, PMCID: PMC3547681.Peer-Reviewed Original ResearchConceptsLevels of glucocorticoidsGonadal functionNormal brain developmentTestes/ovariesLevels of glucoseLife-long effectsFetal exposureMaternal exposureStress-related behaviorsAdrenal functionExogenous glucocorticoidsAdrenal axisGonadal axisNeuroendocrine functionGlucocorticoid inhibitionAnatomical sitesPrenatal stressElevated glucocorticoidsGlucocorticoidsPituitary glandReproductive functionBrain developmentStressful challengesFat breakdownMultiple tissues