2023
High-throughput combined voltage-clamp/current-clamp analysis of freshly isolated neurons
Ghovanloo M, Tyagi S, Zhao P, Kiziltug E, Estacion M, Dib-Hajj S, Waxman S. High-throughput combined voltage-clamp/current-clamp analysis of freshly isolated neurons. Cell Reports Methods 2023, 3: 100385. PMID: 36814833, PMCID: PMC9939380, DOI: 10.1016/j.crmeth.2022.100385.Peer-Reviewed Original ResearchConceptsDorsal root ganglion neuronsCurrent-clamp recordingsCurrent-clamp analysisVoltage-gated sodium channelsPatch-clamp techniqueExcitable cellsGanglion neuronsElectrophysiological recordingsNeuronal cellsNeuronsGold standard methodologySodium channelsCellular levelRobotic instrumentsCellsDrug screeningSame cellsIntact tissueRecordings
2022
Cannabidiol increases gramicidin current in human embryonic kidney cells: An observational study
Ghovanloo MR, Goodchild SJ, Ruben PC. Cannabidiol increases gramicidin current in human embryonic kidney cells: An observational study. PLOS ONE 2022, 17: e0271801. PMID: 35913948, PMCID: PMC9342711, DOI: 10.1371/journal.pone.0271801.Peer-Reviewed Original ResearchConceptsHuman embryonic kidney cellsEmbryonic kidney cellsHEK cell membranesMembrane biophysical propertiesKidney cellsMonomeric proteinCompound-protein interactionsBacterial cellsIon channelsCell membraneBiophysical propertiesLipid membranesFormation of poresCellsLinear gramicidinCannabis plantCBD activityMembraneGramicidinPlantsProteinPotential therapeutic properties