2018
Superior real‐time polymerase chain reaction detection of Babesia microti parasites in whole blood utilizing high‐copy BMN antigens as amplification targets
Grabias B, Clement J, Krause PJ, Lepore T, Kumar S. Superior real‐time polymerase chain reaction detection of Babesia microti parasites in whole blood utilizing high‐copy BMN antigens as amplification targets. Transfusion 2018, 58: 1924-1932. PMID: 29664114, DOI: 10.1111/trf.14642.Peer-Reviewed Original ResearchConceptsParasites/mLBlood donorsBlood samplesSeroreactive antigensBabesia microti parasitesWhole bloodTransfusion-transmitted infectionsParasite nucleic acidsReal-time polymerase chain reactionWhole blood samplesPolymerase chain reaction detectionPolymerase chain reactionSensitive molecular assaysBlood productsClinical characterizationRT-PCR detectionReal-time polymerase chain reaction detectionLaboratory diagnosisBabesia microtiStudy designInfected ticksDilution panelsAntigenZoonotic diseaseBloodHuman Borrelia miyamotoi infection in California: Serodiagnosis is complicated by multiple endemic Borrelia species
Krause PJ, Carroll M, Fedorova N, Brancato J, Dumouchel C, Akosa F, Narasimhan S, Fikrig E, Lane RS. Human Borrelia miyamotoi infection in California: Serodiagnosis is complicated by multiple endemic Borrelia species. PLOS ONE 2018, 13: e0191725. PMID: 29420552, PMCID: PMC5805228, DOI: 10.1371/journal.pone.0191725.Peer-Reviewed Original ResearchConceptsBorrelia miyamotoi infectionMiyamotoi infectionB. miyamotoiB. miyamotoi diseaseB. miyamotoi infectionB. burgdorferi infectionHealth care professionalsWhole cell lysatesSeropositive subjectsSufficient serumClinical dataBurgdorferi infectionAntibody assaysSeropositive samplesCare professionalsBorrelia antigensInfectionSerumAntibodiesBorrelia burgdorferiAntigenI. pacificus ticksMiyamotoiTick infectionFever spirochetes
2014
Borrelia miyamotoi sensu lato Seroreactivity and Seroprevalence in the Northeastern United States - Volume 20, Number 7—July 2014 - Emerging Infectious Diseases journal - CDC
Krause PJ, Narasimhan S, Wormser GP, Barbour AG, Platonov AE, Brancato J, Lepore T, Dardick K, Mamula M, Rollend L, Steeves TK, Diuk-Wasser M, Usmani-Brown S, Williamson P, Sarksyan DS, Fikrig E, Fish D, . Borrelia miyamotoi sensu lato Seroreactivity and Seroprevalence in the Northeastern United States - Volume 20, Number 7—July 2014 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2014, 20: 1183-1190. PMID: 24960072, PMCID: PMC4073859, DOI: 10.3201/eid2007.131587.Peer-Reviewed Original ResearchConceptsB. burgdorferiInfectious Diseases journal - CDCSeroprevalence of IgGB. burgdorferi antigensLyme disease endemic areaDisease-endemic areasAntibody testingHealthy personsLyme diseaseSerum samplesSeroprevalenceBurgdorferiInfectionSame tickUnited StatesSensu latoArea residentsPersonsBorrelia spSeroreactivityIgGAntigenDiseaseSerum
2001
Diagnosis of Babesiosis Using an Immunoblot Serologic Test
Ryan R, Krause P, Radolf J, Freeman K, Spielman A, Lenz R, Levin A. Diagnosis of Babesiosis Using an Immunoblot Serologic Test. MSphere 2001, 8: 1177-1180. PMID: 11687460, PMCID: PMC96246, DOI: 10.1128/cdli.8.6.1177-1180.2001.Peer-Reviewed Original ResearchConceptsB. microti antigenAntibody testHuman babesiosisB. microtiReactivity of seraDiagnostic antibody testsHuman granulocytic ehrlichiosisDiagnosis of babesiosisImmunoblot stripsRoutine clinical diagnosisSerologic testsAcute babesiosisIFA testLaboratory evidenceClinical diagnosisImmunoblot assayGranulocytic ehrlichiosisLyme diseaseAntigenSerumGI strainsInterpretive criteriaReactive bandsTest sensitivityHuman subjects
2000
Serological Expression Cloning of Novel Immunoreactive Antigens of Babesia microti
Lodes M, Houghton R, Bruinsma E, Mohamath R, Reynolds L, Benson D, Krause P, Reed S, Persing D. Serological Expression Cloning of Novel Immunoreactive Antigens of Babesia microti. Infection And Immunity 2000, 68: 2783-2790. PMID: 10768973, PMCID: PMC97488, DOI: 10.1128/iai.68.5.2783-2790.2000.Peer-Reviewed Original ResearchConceptsRelated antigensBabesia microtiSerological expression cloningB. microtiHumoral immune responseB. microti infectionClass of antigensSporozoite surface antigenPatient serum poolsWestern blot analysisBlood transfusionMicroti infectionImmune responseSurface antigenImmunodominant antigensAntigenHuman babesiosisMolecular testsGenomic expression libraryPlasmodium spCausative agentSerum poolsImmunoreactive antigensBlot analysisMicroti
1999
Southern Extension of the Range of Human Babesiosis in the Eastern United States
Eskow E, Krause P, Spielman A, Freeman K, Aslanzadeh J. Southern Extension of the Range of Human Babesiosis in the Eastern United States. Journal Of Clinical Microbiology 1999, 37: 2051-2052. PMID: 10325378, PMCID: PMC85028, DOI: 10.1128/jcm.37.6.2051-2052.1999.Peer-Reviewed Original ResearchConceptsMicroti infectionBlood samplesB. microti antibodiesSerial blood samplesBabesia microti infectionB. microti infectionCollected blood samplesMicroti antibodiesAcute babesiosisImmunofluorescent antibodyHuman B. microti infectionBabesial antigensHuman babesiosisB. microtiInfectionBabesiosisAntibodiesPCR techniqueUnited StatesLocal acquisitionResidentsPatientsSymptomsAntigenBlood
1996
Concurrent Lyme Disease and Babesiosis: Evidence for Increased Severity and Duration of Illness
Krause P, Telford S, Spielman A, Sikand V, Ryan R, Christianson D, Burke G, Brassard P, Pollack R, Peck J, Persing D. Concurrent Lyme Disease and Babesiosis: Evidence for Increased Severity and Duration of Illness. JAMA 1996, 275: 1657-1660. DOI: 10.1001/jama.1996.03530450047031.Peer-Reviewed Original ResearchConcurrent Lyme diseaseDuration of illnessLyme diseaseClinic-based cohort studyBabesial infectionDuration of symptomsNumber of symptomsCoinfected PatientsSpirochete antigenCohort studyIncreased SeverityPatientsMore symptomsMedical clinicsPersistent episodesSymptomsDiseaseSpirochetal DNAEmotional labilityInfectionBabesial antigensIllnessBabesiosisClinicAntigen
1995
Infection with a Babesia-Like Organism in Northern California
Persing D, Herwaldt B, Glaser C, Lane R, Thomford J, Mathiesen D, Krause P, Phillip D, Conrad P. Infection with a Babesia-Like Organism in Northern California. New England Journal Of Medicine 1995, 332: 298-303. PMID: 7816065, DOI: 10.1056/nejm199502023320504.Peer-Reviewed Original ResearchConceptsB. microti antigenIndirect immunofluorescent antibody testingImmunofluorescent antibody testingInfluenza-like illnessHodgkin's diseaseSerologic testingAsymptomatic infectionClinical spectrumSeroprevalence ratesPolymerase chain reactionPatientsFatal diseaseProtozoal parasitesInfectionBabesia microtiHuman babesiosisBabesia antigensDiseaseGenetic sequence analysisChain reactionCanine pathogensAntigenB. divergensGenus Babesia
1990
The Role of Cellular Maturation in Neutrophil Heterogeneity
Krause P, Todd M, Hancock W, Pastuszak W, Maderazo E, Hild D, Kosciol C. The Role of Cellular Maturation in Neutrophil Heterogeneity. Blood 1990, 76: 1639-1646. DOI: 10.1182/blood.v76.8.1639.1639.Peer-Reviewed Original ResearchMaturational differencesPeripheral blood PMNPositive bone marrow cellsFunctional heterogeneityBone marrow cellsFlow cytometric analysisDifferent functional capacitiesBlood PMNMonoclonal antibody labelingNeutrophil heterogeneityPMN subpopulationFunctional capacityAntigenic expressionMyeloid cellsNeutrophil characteristicsFunctional maturationPMN populationCytometric analysisMarrow cellsMorphologic maturationMyeloblast cellsHL-60 cellsAntigenPMNHeterogeneous expressionThe role of cellular maturation in neutrophil heterogeneity.
Krause P, Todd M, Hancock W, Pastuszak W, Maderazo E, Hild D, Kosciol C. The role of cellular maturation in neutrophil heterogeneity. Blood 1990, 76: 1639-46. PMID: 2207334, DOI: 10.1182/blood.v76.8.1639.bloodjournal7681639.Peer-Reviewed Original ResearchConceptsMaturational differencesPositive bone marrow cellsPeripheral blood PMNFunctional heterogeneityBone marrow cellsFlow cytometric analysisDifferent functional capacitiesBlood PMNMonoclonal antibody labelingNeutrophil heterogeneityPMN subpopulationFunctional capacityAntigenic expressionMyeloid cellsNeutrophil characteristicsFunctional maturationPMN populationCytometric analysisMorphologic maturationMarrow cellsMyeloblast cellsHL-60 cellsAntigenPMNHeterogeneous expression
1985
1001 NEUTROPHIL HETEROGENEITY IN NEONATES AND ADULTS
Krause P, Kosciol C, Pontius L, Malech H, Rave J. 1001 NEUTROPHIL HETEROGENEITY IN NEONATES AND ADULTS. Pediatric Research 1985, 19: 277-277. DOI: 10.1203/00006450-198504000-01031.Peer-Reviewed Original ResearchAdult PMNHealthy neonatesNeutrophil heterogeneityPMN chemotaxisNeonatesUnseparated populationsResponsive cellsChemotactic chambersPMNMajor subpopulationsIndirect fluorescent labelingMyelocyte stageAdultsFunctional significanceCellsSubpopulationsLarge percentageLabeling patternsMajor peakNewbornsNeutrophilsAntigenFMLP