2024
Microscopic phage adsorption assay: High-throughput quantification of virus particle attachment to host bacterial cells
Antani J, Ward T, Emonet T, Turner P. Microscopic phage adsorption assay: High-throughput quantification of virus particle attachment to host bacterial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2410905121. PMID: 39700139, PMCID: PMC11670125, DOI: 10.1073/pnas.2410905121.Peer-Reviewed Original ResearchMeSH KeywordsAdsorptionBacteriaBacteriophagesEscherichia coliHigh-Throughput Screening AssaysMicroscopy, FluorescenceVirionVirus AttachmentConceptsBacterial cellsViruses of bacteriaHost bacterial cellsTarget bacterial strainsEnumeration of bacteriaPhage biologyPhage attachmentHigh-throughput quantificationPhage libraryBacterial pathogensHigh-throughput screeningPhageBacterial strainsSingle-virusVirus particlesIndividual virus particlesFluorescence microscopyBacteriaEarth's biosphereParticle trackingCellsLow throughputBacteriumRate constantsPathogens
2011
High-throughput analysis of growth differences among phage strains
Turner PE, Draghi JA, Wilpiszeski R. High-throughput analysis of growth differences among phage strains. Journal Of Microbiological Methods 2011, 88: 117-121. PMID: 22101310, DOI: 10.1016/j.mimet.2011.10.020.Peer-Reviewed Original ResearchMeSH KeywordsBacteriophage phi 6Computer SimulationHigh-Throughput Screening AssaysPseudomonas syringaeSpectrophotometryConceptsFitness differencesHigh-throughput analysisGrowth differencesPhage strainsAbsolute fitnessHigh-throughput methodBacterial hostsPhage growthGenotype rankingsPhi 6Phage phi 6Bacterial strainsThroughput methodViral growthVirus strainsBacterial growth curveHostBacteriophage strainsStrainsGrowthVitro methodGrowth curvesTraitsPhagesThroughput analysis
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