2013
Secretome analysis of Glioblastoma cell line - HNGC-2
Gupta M, Polisetty R, Ramamoorthy K, Tiwary S, Kaur N, Uppin M, Shiras A, Sirdeshmukh R. Secretome analysis of Glioblastoma cell line - HNGC-2. Molecular Omics 2013, 9: 1390-1400. PMID: 23483059, DOI: 10.1039/c3mb25383j.Peer-Reviewed Original ResearchConceptsHNGC-2 cellsProtein identificationActin cytoskeleton signalingClassical secretory pathwayPhosphatidyl inositol 3 kinaseCell linesESI-IT mass spectrometerCytoskeleton signalingTransmembrane domainSecretory pathwayDNA recombinationSignal sequenceCellular assemblyPlasma membraneNon-redundant listLC-MS/MS analysisSecretome analysisExtracellular localizationGlioblastoma cellsSDS gelsGlioblastoma multiformeProteinImportant functional groupsSecretomeKinase
2010
Dlxin-1, a member of MAGE family, inhibits cell proliferation, invasion and tumorigenicity of glioma stem cells
Reddy E, Chettiar S, Kaur N, Ganeshkumar R, Shepal V, Shanbhag N, Shiras A. Dlxin-1, a member of MAGE family, inhibits cell proliferation, invasion and tumorigenicity of glioma stem cells. Cancer Gene Therapy 2010, 18: 206-218. PMID: 21109781, DOI: 10.1038/cgt.2010.71.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCell MovementCell ProliferationCyclin-Dependent Kinase Inhibitor p21Down-RegulationFemaleGene Expression Regulation, NeoplasticGliomaHumansMatrix Metalloproteinase 2Melanoma-Specific AntigensMiceMice, SCIDNeoplastic Stem CellsNerve Tissue ProteinsOncogene ProteinsSignal TransductionXenograft Model Antitumor AssaysConceptsGlioma stem cellsStem cellsBrain tumor stem cellsCancer-initiating stem cellsHigh-grade gliomasEffective therapeutic targetingAnti-proliferative effectsGlioma cell invasionMelanoma antigen gene (MAGE) familyCancer stem cell hypothesisInhibits cell proliferationTumor stem cellsCell cycle arrest proteinsInducers of differentiationMMP-9Brain tumorsStem cell hypothesisMMP-2Therapeutic targetingAnti-proliferative functionDlxin-1MAGE familyHNGC-2 cellsCell invasionCell proliferationDlxin-1, a MAGE family protein, induces accelerated neurite outgrowth and cell survival by enhanced and early activation of MEK and Akt signalling pathways in PC12 cells
Reddy E, Chettiar S, Kaur N, Shepal V, Shiras A. Dlxin-1, a MAGE family protein, induces accelerated neurite outgrowth and cell survival by enhanced and early activation of MEK and Akt signalling pathways in PC12 cells. Experimental Cell Research 2010, 316: 2220-2236. PMID: 20595047, DOI: 10.1016/j.yexcr.2010.05.030.Peer-Reviewed Original ResearchConceptsDlxin-1MAGE homology domainCell survivalPC12 cellsPresence of NGFNeuronal differentiationDiverse cellular functionsMAGE family proteinsCell cycle progressionTranscriptional regulationHomology domainCellular functionsFamily proteinsNeuronal survivalDevelopmental apoptosisEnhanced neuritogenesisCycle progressionSignaling pathwaysMEK pathwayPharmacological inhibitorsCell deathAkt pathwayUnique regionAmino acidsEarly activation