2024
Neuropeptide and serotonin co-transmission sets the activity pattern in the C. elegans egg-laying circuit
Butt A, Van Damme S, Santiago E, Olson A, Beets I, Koelle M. Neuropeptide and serotonin co-transmission sets the activity pattern in the C. elegans egg-laying circuit. Current Biology 2024, 34: 4704-4714.e5. PMID: 39395419, DOI: 10.1016/j.cub.2024.07.064.Peer-Reviewed Original ResearchHermaphrodite-specific neuronsEgg-laying circuitNlp-3Egg layingTiming of egg layingPromote egg layingCaenorhabditis elegansInactive phasePeptide signalsPersistent internal stateNeural circuit functionMolecular experimentsSerotonin receptorsSerotonin signalingSerotoninMuscle cellsNeurotransmitter serotoninMultiple signalsNeuropeptideNeuropeptide receptorsCo-transmissionEggsNeural circuitsLayingActive phase
2019
Serotonin and neuropeptides are both released by the HSN command neuron to initiate C. elegans egg laying
Brewer JC, Olson AC, Collins KM, Koelle MR. Serotonin and neuropeptides are both released by the HSN command neuron to initiate C. elegans egg laying. PLOS Genetics 2019, 15: e1007896. PMID: 30677018, PMCID: PMC6363226, DOI: 10.1371/journal.pgen.1007896.Peer-Reviewed Original ResearchConceptsHermaphrodite-specific neuronsEgg-laying defectsNLP-3C. elegansEgg-laying musclesEgg-laying circuitDirect postsynaptic targetsEgg-laying behaviorSerotonergic Hermaphrodite Specific NeuronsMuscle cellsSmall molecule neurotransmittersNull mutantsHSN neuronsDouble mutantSingle mutantsMutant animalsSerotonergic neuronsWild-type animalsSevere defectsMutantsElegansNeuropeptide substance PMammalian brainEggsSpecific neurons
2013
Postsynaptic ERG Potassium Channels Limit Muscle Excitability to Allow Distinct Egg-Laying Behavior States in Caenorhabditis elegans
Collins KM, Koelle MR. Postsynaptic ERG Potassium Channels Limit Muscle Excitability to Allow Distinct Egg-Laying Behavior States in Caenorhabditis elegans. Journal Of Neuroscience 2013, 33: 761-775. PMID: 23303953, PMCID: PMC3542984, DOI: 10.1523/jneurosci.3896-12.2013.Peer-Reviewed Original ResearchConceptsEgg-laying musclesBody bendsUNC-103Caenorhabditis elegansCalcium transientsTwo-state behaviorERG potassium channelsMutantsERG channelsPotassium channelsInactive phaseEggsDistinct behavioral statesMuscle excitabilityPostsynaptic excitabilityPostsynaptic sitesERG functionCaenorhabditisCalcium imagingElegansFurther adjustmentExcitabilityRhythmic excitationMuscleBehavioral states
2008
Regulation of Serotonin Biosynthesis by the G Proteins Gαo and Gαq Controls Serotonin Signaling in Caenorhabditis elegans
Tanis JE, Moresco JJ, Lindquist RA, Koelle MR. Regulation of Serotonin Biosynthesis by the G Proteins Gαo and Gαq Controls Serotonin Signaling in Caenorhabditis elegans. Genetics 2008, 178: 157-169. PMID: 18202365, PMCID: PMC2206068, DOI: 10.1534/genetics.107.079780.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersCaenorhabditis elegansCaenorhabditis elegans ProteinsGene Expression Regulation, EnzymologicGTP-Binding Protein alpha Subunits, Gi-GoGTP-Binding Protein alpha Subunits, Gq-G11Motor NeuronsMusclesOrgan SpecificityOvipositionPromoter Regions, GeneticSerotoninSignal TransductionSynapsesTryptophan HydroxylaseConceptsEgg-laying behaviorCaenorhabditis elegansG proteinsEgg-laying systemHSN motor neuronsSerotonin biosynthesisG protein GαoLevel of transcriptionEpistasis experimentsTPH-1 expressionRate-limiting enzymeGene dosageSpecific genesGalphaoGalphaqTranscriptionBiosynthesisMotor neuronsElegansTPH-1Neurotransmitter releaseGenesMultiple signalsProteinEggs
2007
Biogenic amine neurotransmitters in C. elegans.
Chase DL, Koelle MR. Biogenic amine neurotransmitters in C. elegans. WormBook 2007, 1-15. PMID: 18050501, PMCID: PMC4781333, DOI: 10.1895/wormbook.1.132.1.Peer-Reviewed Original Research
2004
Activation of EGL-47, a Gαo-Coupled Receptor, Inhibits Function of Hermaphrodite-Specific Motor Neurons to Regulate Caenorhabditis elegans Egg-Laying Behavior
Moresco JJ, Koelle MR. Activation of EGL-47, a Gαo-Coupled Receptor, Inhibits Function of Hermaphrodite-Specific Motor Neurons to Regulate Caenorhabditis elegans Egg-Laying Behavior. Journal Of Neuroscience 2004, 24: 8522-8530. PMID: 15456826, PMCID: PMC6729914, DOI: 10.1523/jneurosci.1915-04.2004.Peer-Reviewed Original ResearchConceptsHermaphrodite-specific neuronsEgg-laying behaviorTransmembrane domainEgg-laying defectsEgg-laying musclesDominant mutationsHSN motor neuronsFluorescent protein transgeneSixth transmembrane domainExtracellular N-terminusEgg-laying frequencyMotor neuronsN-terminusG proteinsTransgenic expressionGenesCaenorhabditisMild defectsReceptor isoformsInhibits functionMutationsMultiple receptorsEggsReceptorsNumber of neurons
2003
Genetic and Cellular Basis for Acetylcholine Inhibition of Caenorhabditis elegans Egg-Laying Behavior
Bany IA, Dong MQ, Koelle MR. Genetic and Cellular Basis for Acetylcholine Inhibition of Caenorhabditis elegans Egg-Laying Behavior. Journal Of Neuroscience 2003, 23: 8060-8069. PMID: 12954868, PMCID: PMC6740490, DOI: 10.1523/jneurosci.23-22-08060.2003.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAcetylcholinesteraseAnimalsAnimals, Genetically ModifiedBehavior, AnimalCaenorhabditis elegansCaenorhabditis elegans ProteinsCholinesterase InhibitorsDNA, ComplementaryGTP-Binding ProteinsHomeodomain ProteinsInhibition, PsychologicalMutationNeuronsNuclear ProteinsOvipositionPhenotypeReceptors, CholinergicSignal TransductionSynapsesConceptsHermaphrodite-specific neuronsEgg-laying behaviorG proteinsG-protein signaling genesEgg-laying defectsEgg-laying musclesEgg-laying systemAnalysis of mutantsInhibition of eggSerotonergic Hermaphrodite Specific NeuronsUnc-4Caenorhabditis elegansUnc-17Signaling GenesThird cell typeActivation of eggsMorphological defectsCha-1MutantsCell typesCellular basisNeurotransmitter releaseGenesEggsPartial defect
2000
Multiple RGS proteins alter neural G protein signaling to allow C. elegans to rapidly change behavior when fed
Dong M, Chase D, Patikoglou G, Koelle M. Multiple RGS proteins alter neural G protein signaling to allow C. elegans to rapidly change behavior when fed. Genes & Development 2000, 14: 2003-2014. PMID: 10950865, PMCID: PMC316861, DOI: 10.1101/gad.14.16.2003.Peer-Reviewed Original ResearchConceptsRGS proteinsEGL-10Egg-laying behaviorG proteinsRGS-2RGS-1Mammalian RGS proteinsMultiple RGS proteinsHeterotrimeric G proteinsG protein GTPase activityG protein signalingProtein GTPase activityGTPase activatorCaenorhabditis elegansC. elegansRGS genesDouble mutantProtein signalingGTPase activityProteinElegansBiological purposesRegulatorAppropriate behavioral responsesEggs