2025
A quantitative ultrastructural timeline of nuclear autophagy reveals a role for dynamin-like protein 1 at the nuclear envelope
Mannino P, Perun A, Surovtsev I, Ader N, Shao L, Rodriguez E, Melia T, King M, Lusk C. A quantitative ultrastructural timeline of nuclear autophagy reveals a role for dynamin-like protein 1 at the nuclear envelope. Nature Cell Biology 2025, 27: 464-476. PMID: 39920277, PMCID: PMC11908896, DOI: 10.1038/s41556-025-01612-1.Peer-Reviewed Original ResearchConceptsInner nuclear membraneDynamin-like protein 1Membrane fissionNuclear envelopeMembrane fission stepNon-canonical rolesDouble-membrane vesiclesProtein 1Nuclear envelope remodelingLattice light-sheet microscopyFission stepDnm1Nuclear autophagyIntact nucleiPerinuclear spaceNuclear membraneAutophagic mechanismsNucleophagyCorrelative lightLight-sheet microscopyFissionElectron tomographyVesiclesVacuolesAtg11
2024
A-tisket, a-tasket, what a beautiful nuclear basket
Lusk C, King M. A-tisket, a-tasket, what a beautiful nuclear basket. Cell 2024, 187: 5225-5227. PMID: 39303690, DOI: 10.1016/j.cell.2024.08.030.Peer-Reviewed Original Research
2023
Dynamic regulation of LINC complex composition and function across tissues and contexts
King M. Dynamic regulation of LINC complex composition and function across tissues and contexts. FEBS Letters 2023, 597: 2823-2832. PMID: 37846646, DOI: 10.1002/1873-3468.14757.Peer-Reviewed Original ResearchMeSH KeywordsCell NucleusCytoskeletonMechanotransduction, CellularMicrotubulesNuclear EnvelopeNuclear MatrixConceptsLINC complex componentsLINC complexLinker of nucleoskeletonProtein turnover mechanismsCytoplasmic intermediate filament networkIntermediate filament networkCytoskeleton (LINC) complexComplex componentsNuclear laminaCellular contextCytoplasmic cytoskeletonCell biologistsDynamic regulationNuclear envelopeRegulated expressionTurnover mechanismsFilament networkSplice variantsCell typesProtein configurationUnique functionCytoskeletonConcept of mechanotransductionComplexesNucleoskeleton
2015
The tethering of chromatin to the nuclear envelope supports nuclear mechanics
Schreiner SM, Koo PK, Zhao Y, Mochrie SG, King MC. The tethering of chromatin to the nuclear envelope supports nuclear mechanics. Nature Communications 2015, 6: 7159. PMID: 26074052, PMCID: PMC4490570, DOI: 10.1038/ncomms8159.Peer-Reviewed Original ResearchConceptsNuclear mechanicsRole of chromatinOptical tweezersWild-type nucleiNetwork of lipidsCytoskeletal forcesNuclear laminaCytoskeletal dynamicsMechanical defensesMembrane tethersDeformable nucleiNuclear envelopeChromatinNuclear shapeIsolated nucleiIndividual mechanical contributionsMembrane resultsNucleusTweezersMechanicsLaminsProteinTetheringLaminaDefenseNuclear–cytoskeletal linkages facilitate cross talk between the nucleus and intercellular adhesions
Stewart RM, Zubek AE, Rosowski KA, Schreiner SM, Horsley V, King MC. Nuclear–cytoskeletal linkages facilitate cross talk between the nucleus and intercellular adhesions. Journal Of Cell Biology 2015, 209: 403-418. PMID: 25963820, PMCID: PMC4427780, DOI: 10.1083/jcb.201502024.Peer-Reviewed Original ResearchConceptsIntercellular adhesionNuclear positionEpidermal tissue integrityLinker of nucleoskeletonCross talkPrimary mouse keratinocytesCytoskeleton (LINC) complexCytoplasmic cytoskeletonAdhesion functionMicrotubule networkDefective adhesionCytoskeletonSUN2Mouse keratinocytesTissue integrityFollicle structureEpidermal keratinocytesAdhesionNucleoskeletonCellsKeratinocytesAdhesion formationNucleusIntegrityComplexes
2008
A Network of Nuclear Envelope Membrane Proteins Linking Centromeres to Microtubules
King MC, Drivas TG, Blobel G. A Network of Nuclear Envelope Membrane Proteins Linking Centromeres to Microtubules. Cell 2008, 134: 427-438. PMID: 18692466, PMCID: PMC2617791, DOI: 10.1016/j.cell.2008.06.022.Peer-Reviewed Original ResearchConceptsSUN-KASH complexesNuclear membrane proteinsMembrane proteinsIntegral inner nuclear membrane proteinsSUN domain protein Sad1Nuclear envelopeSpindle pole body componentInner nuclear membrane proteinFission yeast S. pombeOuter nuclear membrane proteinsCytoplasmic microtubulesYeast S. pombeMicrotubule-dependent forcesNuclear envelope membranesCentromeric DNAS. pombeCentromeric heterochromatinHeterochromatic regionsEnvelope membraneIMA1MicrotubulesSad1HeterochromatinProteinNuclear heterochromatin
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