Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling
Hsu JC, Pawlak JB, Laurent-Rolle M, Cresswell P. Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling. STAR Protocols 2022, 3: 101654. PMID: 36072758, PMCID: PMC9442383, DOI: 10.1016/j.xpro.2022.101654.Peer-Reviewed Original ResearchConceptsTranslational regulationMammalian cell linesDifferent cell typesDifferent growth conditionsMammalian cellsTranslation activityGene expressionBiological processesCell typesComplete detailsConfocal microscopyCell linesGrowth conditionsPuromycin analoguesCritical roleRegulationCellsProteinLabelingFundamental stepExpressionClick chemistry reactionActivityCytometry
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