2024
High burden of viruses and bacterial pathobionts drives heightened nasal innate immunity in children
Watkins T, Green A, Amat J, Cheemarla N, Hänsel K, Lozano R, Dudgeon S, Germain G, Landry M, Schulz W, Foxman E. High burden of viruses and bacterial pathobionts drives heightened nasal innate immunity in children. Journal Of Experimental Medicine 2024, 221: e20230911. PMID: 38949638, PMCID: PMC11215523, DOI: 10.1084/jem.20230911.Peer-Reviewed Original ResearchConceptsBacterial pathobiontsRespiratory virusesBurden of virusesSARS-CoV-2Innate immune activationSARS-CoV-2 viral loadDynamic host-pathogen interactionsInnate immune responseViral coinfectionCytokine profileViral loadNasal virusImmune activationProinflammatory responseIL-1BNasopharyngeal samplesHost-pathogen interactionsImmune responseInterferon responsePathobiontsInnate immunityPaired samplesCXCL10Healthy 1-year-oldVirus
2021
Dynamic innate immune response determines susceptibility to SARS-CoV-2 infection and early replication kinetics
Cheemarla NR, Watkins TA, Mihaylova VT, Wang B, Zhao D, Wang G, Landry ML, Foxman EF. Dynamic innate immune response determines susceptibility to SARS-CoV-2 infection and early replication kinetics. Journal Of Experimental Medicine 2021, 218: e20210583. PMID: 34128960, PMCID: PMC8210587, DOI: 10.1084/jem.20210583.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overAngiotensin-Converting Enzyme 2Case-Control StudiesChemokine CXCL10COVID-19Disease SusceptibilityFemaleGene Expression ProfilingHost-Pathogen InteractionsHumansImmunity, InnateInterferonsMaleMiddle AgedNasopharynxPicornaviridae InfectionsSARS-CoV-2Viral LoadVirus ReplicationConceptsSARS-CoV-2 infectionSARS-CoV-2 exposureSARS-CoV-2Interferon-stimulated genesUpper respiratory tractRespiratory tractEarly SARS-CoV-2 infectionDynamic innate immune responseViral replicationSARS-CoV-2 replicationPatient nasopharyngeal samplesInnate immune responseLow infectious doseViral loadNasopharyngeal samplesImmune responseInfectious doseISG responseAntiviral responseInfection progressionViral transmissionLevel correlatesInfectionISG inductionInitial replicationReal-world assessment of severe acute respiratory coronavirus virus 2 (SARS-CoV-2) nasopharyngeal swab testing in a region with a high burden of coronavirus disease 2019 (COVID-19)
Sangal RB, Peaper DR, Rothenberg C, Fadlallah H, Mobolaji-Lawal M, Landry ML, Sussman LS, Ulrich A, Venkatesh AK. Real-world assessment of severe acute respiratory coronavirus virus 2 (SARS-CoV-2) nasopharyngeal swab testing in a region with a high burden of coronavirus disease 2019 (COVID-19). Infection Control And Hospital Epidemiology 2021, 43: 1051-1053. PMID: 33823949, PMCID: PMC8111191, DOI: 10.1017/ice.2021.153.Peer-Reviewed Original Research
2020
Challenges in use of saliva for detection of SARS CoV-2 RNA in symptomatic outpatients
Landry ML, Criscuolo J, Peaper DR. Challenges in use of saliva for detection of SARS CoV-2 RNA in symptomatic outpatients. Journal Of Clinical Virology 2020, 130: 104567. PMID: 32750665, PMCID: PMC7392849, DOI: 10.1016/j.jcv.2020.104567.Peer-Reviewed Original ResearchMeSH KeywordsBetacoronavirusClinical Laboratory TechniquesCoronavirus InfectionsCOVID-19COVID-19 TestingCOVID-19 VaccinesHumansMolecular Diagnostic TechniquesNasopharynxOutpatientsPandemicsPneumonia, ViralProspective StudiesReal-Time Polymerase Chain ReactionRNA, ViralSalivaSARS-CoV-2Specimen HandlingConceptsNasopharyngeal swabsReal-time RT-PCRSymptomatic outpatientsRT-PCRSARS-CoV-2 positivitySaliva samplesSARS-CoV-2 testingMedian cycle threshold valuePure salivaSARS-CoV-2 RNA detectionSARS-CoV-2 RNACoV-2 testingUse of salivaCycle threshold valuesSymptomatic patientsOverall sensitivityRNA detectionSalivaCOVID-19OutpatientsHigh-volume testingPatientsSwabs
2018
Detection of Influenza A and B Viruses and Respiratory Syncytial Virus by Use of Clinical Laboratory Improvement Amendments of 1988 (CLIA)-Waived Point-of-Care Assays: a Paradigm Shift to Molecular Tests
Azar MM, Landry ML. Detection of Influenza A and B Viruses and Respiratory Syncytial Virus by Use of Clinical Laboratory Improvement Amendments of 1988 (CLIA)-Waived Point-of-Care Assays: a Paradigm Shift to Molecular Tests. Journal Of Clinical Microbiology 2018, 56: 10.1128/jcm.00367-18. PMID: 29695519, PMCID: PMC6018333, DOI: 10.1128/jcm.00367-18.BooksConceptsRapid influenza diagnostic testsRespiratory syncytial virusNucleic acid amplification testsSyncytial virusB virusInfluenza AClinical Laboratory Improvement AmendmentsAntiviral therapyPoint of careAvailable rapid influenza diagnostic testsSensitive nucleic acid amplification testsMolecular testsInfluenza diagnostic testsRespiratory virus infectionsInfection prevention strategiesRapid antigen detectionRapid laboratory testingViral diagnostic testingDetection of influenzaNew pandemic strainsAccurate laboratory diagnosisRapid antigenRespiratory virusesViral culturePatient management
2017
Antiviral Response in the Nasopharynx Identifies Patients With Respiratory Virus Infection
Landry ML, Foxman EF. Antiviral Response in the Nasopharynx Identifies Patients With Respiratory Virus Infection. The Journal Of Infectious Diseases 2017, 217: 897-905. PMID: 29281100, PMCID: PMC5853594, DOI: 10.1093/infdis/jix648.Peer-Reviewed Original ResearchConceptsRespiratory virusesNasopharyngeal swabsViral infectionCXCL10 protein levelsPatient nasopharyngeal swabsRespiratory virus infectionsHuman nasal epithelial cellsManagement of patientsRespiratory virus detectionNasal epithelial cellsSingle host proteinVirus detectionSimple diagnostic testIdentifies patientsRespiratory symptomsRespiratory infectionsRespiratory illnessHigh burdenVirus infectionReceptor RIGCost-effective testAntiviral responseAccurate diagnosisDiagnostic testsInfection
2014
Comparison of Simplexa Flu A/B & RSV PCR with Cytospin-Immunofluorescence and Laboratory-Developed TaqMan PCR in Predominantly Adult Hospitalized Patients
Landry ML, Ferguson D. Comparison of Simplexa Flu A/B & RSV PCR with Cytospin-Immunofluorescence and Laboratory-Developed TaqMan PCR in Predominantly Adult Hospitalized Patients. Journal Of Clinical Microbiology 2014, 52: 3057-3059. PMID: 24850350, PMCID: PMC4136128, DOI: 10.1128/jcm.00738-14.Peer-Reviewed Original Research
2009
Cytospin-Enhanced Immunofluorescence and Impact of Sample Quality on Detection of Novel Swine Origin (H1N1) Influenza Virus
Landry ML, Ferguson D. Cytospin-Enhanced Immunofluorescence and Impact of Sample Quality on Detection of Novel Swine Origin (H1N1) Influenza Virus. Journal Of Clinical Microbiology 2009, 48: 957-959. PMID: 20042632, PMCID: PMC2832425, DOI: 10.1128/jcm.01678-09.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAnimalsChildChild, PreschoolClinical Laboratory TechniquesFluorescent Antibody Technique, DirectHumansInfantInfant, NewbornInfluenza A Virus, H1N1 SubtypeInfluenza, HumanNasopharynxReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityVirologyYoung AdultConceptsNovel swine-origin influenza virusesSwine-origin influenza virusImmunofluorescence antibody testingReal-time reverse transcriptase PCRCycle threshold valuesPCR-positive resultsPediatric patientsCDC assayAntibody testingNasopharyngeal swabsReverse transcriptase-PCRSwine influenzaInfluenza virusTranscriptase-PCRPositive samplesPatientsPositive resultsInfluenzaSwabsCytospins
2008
Real-time PCR compared to Binax NOW and cytospin-immunofluorescence for detection of influenza in hospitalized patients
Landry ML, Cohen S, Ferguson D. Real-time PCR compared to Binax NOW and cytospin-immunofluorescence for detection of influenza in hospitalized patients. Journal Of Clinical Virology 2008, 43: 148-151. PMID: 18639488, PMCID: PMC7173065, DOI: 10.1016/j.jcv.2008.06.006.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overAntibodies, MonoclonalChildChild, PreschoolFluorescent Antibody Technique, DirectHospitalizationHumansImmunoassayInfantInfluenza A virusInfluenza B virusInfluenza, HumanMiddle AgedNasopharynxReagent Kits, DiagnosticReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityTaq PolymeraseTime FactorsVirus CultivationYoung AdultConceptsHospitalized patientsTaqMan RT-PCRRT-PCRInfluenza ARapid flu testsDiagnosis of influenzaInfluenza-positive samplesDetection of influenzaReal-time RT-PCRInfluenza BInfluenza diagnosisPatient managementReal-time PCRMore accurate testsPatientsBinaxTrue positive samplesRapid testClinical samplesPositive samplesFurther studiesHospitalAccurate testInfluenzaDiagnosis
2003
Comparison of SmartCycler Real-Time Reverse Transcription-PCR Assay in a Public Health Laboratory with Direct Immunofluorescence and Cell Culture Assays in a Medical Center for Detection of Influenza A Virus
Habib-Bein NF, Beckwith WH, Mayo D, Landry ML. Comparison of SmartCycler Real-Time Reverse Transcription-PCR Assay in a Public Health Laboratory with Direct Immunofluorescence and Cell Culture Assays in a Medical Center for Detection of Influenza A Virus. Journal Of Clinical Microbiology 2003, 41: 3597-3601. PMID: 12904361, PMCID: PMC179819, DOI: 10.1128/jcm.41.8.3597-3601.2003.Peer-Reviewed Original ResearchConceptsVirus-positive specimensDirect immunofluorescence antibody stainingPublic health laboratoriesHealth laboratoriesRT-PCRMedical CenterRapid diagnosisYale-New Haven HospitalReal-time RT-PCRResults of cultureRhesus monkey kidney cell culturesImmunofluorescence antibody stainingReal-time reverse transcriptionInfluenza A virusesMonkey kidney cell culturesConventional RT-PCR analysisReal-time RT-PCR systemRespiratory specimensInfluenza APatient managementDirect immunofluorescenceCell culturesKidney cell culturesRapid turnaround timeA virusSuboptimal Detection of Influenza Virus in Adults by the Directigen Flu A+B Enzyme Immunoassay and Correlation of Results with the Number of Antigen-Positive Cells Detected by Cytospin Immunofluorescence
Landry ML, Ferguson D. Suboptimal Detection of Influenza Virus in Adults by the Directigen Flu A+B Enzyme Immunoassay and Correlation of Results with the Number of Antigen-Positive Cells Detected by Cytospin Immunofluorescence. Journal Of Clinical Microbiology 2003, 41: 3407-3409. PMID: 12843105, PMCID: PMC165325, DOI: 10.1128/jcm.41.7.3407-3409.2003.Peer-Reviewed Original ResearchConceptsVirus infectionEnzyme immunoassayDirectigen FluParainfluenza virus infectionPositive EIA resultsRespiratory syncytial virusAntigen-positive cellsNasopharyngeal swabsSyncytial virusEmergency departmentInfluenza diagnosisDirect immunofluorescenceInfluenza virusVirology laboratoryEIA resultsNight shiftsInfectionInfluenzaImmunofluorescenceFluAdultsVirusCorrelation of resultsImmunoassayCells
2000
Impact of Sample Type on Rapid Detection of Influenza Virus A by Cytospin-Enhanced Immunofluorescence and Membrane Enzyme-Linked Immunosorbent Assay
Landry M, Cohen S, Ferguson D. Impact of Sample Type on Rapid Detection of Influenza Virus A by Cytospin-Enhanced Immunofluorescence and Membrane Enzyme-Linked Immunosorbent Assay. Journal Of Clinical Microbiology 2000, 38: 429-430. PMID: 10618134, PMCID: PMC88742, DOI: 10.1128/jcm.38.1.429-430.2000.Peer-Reviewed Original Research