2023
Biomolecular Condensation of SH2 Domain-Containing Proteins on Membranes
Zeng L, Su X. Biomolecular Condensation of SH2 Domain-Containing Proteins on Membranes. Methods In Molecular Biology 2023, 2705: 371-379. PMID: 37668985, DOI: 10.1007/978-1-0716-3393-9_20.Peer-Reviewed Original ResearchConceptsSH2 domainSH2 domain-containing proteinsDomain-containing proteinsT cell receptorReceptors/adaptorsReceptor pathwayContext of membraneEndomembrane systemMembrane receptor pathwaysSignal transductionBiomolecular condensationSpecific tyrosinePlasma membraneReconstitution systemGolgi apparatusEndoplasmic reticulumLiquid-liquid phase separationMultivalent interactionsCondensate formationProteinMembranePathwayPhosphotyrosineAssaysTransduction
2021
PLCγ1 promotes phase separation of T cell signaling components
Zeng L, Palaia I, Šarić A, Su X. PLCγ1 promotes phase separation of T cell signaling components. Journal Of Cell Biology 2021, 220: e202009154. PMID: 33929486, PMCID: PMC8094118, DOI: 10.1083/jcb.202009154.Peer-Reviewed Original ResearchConceptsTCR signal transductionT-cell receptor pathwayLiquid-like condensatesKey adaptor proteinLAT clusteringLAT complexSH2 domainAdaptor proteinPhosphatase CD45Signal transductionTCR pathwayPhospholipase Cγ1ERK activationProtein compositionBiophysical principlesPLCγ1Critical functionsReceptor pathwayPathwayLATMajor componentT cellsCellsActivationDephosphorylation