2024
Tlr9 deficiency in B cells leads to obesity by promoting inflammation and gut dysbiosis
Wang P, Yang X, Zhang L, Sha S, Huang J, Peng J, Gu J, Pearson J, Hu Y, Zhao H, Wong F, Wang Q, Wen L. Tlr9 deficiency in B cells leads to obesity by promoting inflammation and gut dysbiosis. Nature Communications 2024, 15: 4232. PMID: 38762479, PMCID: PMC11102548, DOI: 10.1038/s41467-024-48611-8.Peer-Reviewed Original ResearchConceptsToll-like receptor 9Gut microbiotaGut microbial communityTransferred to germ-free miceB cellsGerm-free miceTLR9 deficiencyKO miceGene sequencesGerminal center B cellsMicrobial communitiesMarginal zone B cellsGut dysbiosisFollicular helper cellsSelf-DNAMetabolic homeostasisAssociated with increased frequencyPro-inflammatory stateFat tissue inflammationGutHigh-fat dietMicrobiotaHelper cellsT cellsControl mice
2023
Differences in clinical features and gut microbiota between individuals with methamphetamine casual use and methamphetamine use disorder
He L, Yang B, Ma Y, Wen L, Liu F, Zhang X, Liu T. Differences in clinical features and gut microbiota between individuals with methamphetamine casual use and methamphetamine use disorder. Frontiers In Cellular And Infection Microbiology 2023, 13: 1103919. PMID: 36909722, PMCID: PMC9996337, DOI: 10.3389/fcimb.2023.1103919.Peer-Reviewed Original ResearchMeSH KeywordsAmphetamine-Related DisordersAppetiteGastrointestinal MicrobiomeHumansMethamphetamineSubstance Withdrawal SyndromeConceptsMA use disorderClinical featuresGut microbiotaWithdrawal symptomsMA usersEnterotype 2Use disordersGM diversityBody mass indexMethamphetamine use disorderSemi-Structured AssessmentMass indexNeuropsychiatric symptomsHigh incidenceDiagnostic criteriaDifferential microbesDistinct enterotypesDrug dependenceGM differencesSymptomsClinical traitsPerformed network analysisStrong cravingsFecal samplesFurther studies
2021
IL-10 Deficiency Accelerates Type 1 Diabetes Development via Modulation of Innate and Adaptive Immune Cells and Gut Microbiota in BDC2.5 NOD Mice
Huang J, Tan Q, Tai N, Pearson JA, Li Y, Chao C, Zhang L, Peng J, Xing Y, Zhang L, Hu Y, Zhou Z, Wong FS, Wen L. IL-10 Deficiency Accelerates Type 1 Diabetes Development via Modulation of Innate and Adaptive Immune Cells and Gut Microbiota in BDC2.5 NOD Mice. Frontiers In Immunology 2021, 12: 702955. PMID: 34394099, PMCID: PMC8362616, DOI: 10.3389/fimmu.2021.702955.Peer-Reviewed Original ResearchMeSH KeywordsAdaptive ImmunityAnimalsDiabetes Mellitus, Type 1Gastrointestinal MicrobiomeImmunity, InnateInterleukin-10MiceMice, Inbred NODMice, KnockoutT-Lymphocytes, RegulatoryConceptsNOD miceProportion of neutrophilsT cellsGut microbiotaDiabetes developmentT cell-mediated destructionT cell receptor transgenicType 1 diabetes developmentAccelerated diabetes developmentInhibition of diabetesModulation of InnatePathogenicity of CD4Cell-mediated destructionAdaptive immune cellsObese diabetic miceT regulatory (Treg) cellsDevelopment of diabetesPrevention of diabetesActivation of CD4Modulation of neutrophilsType 1 diabetesGut microbiota compositionInsulin-producing β-cellsSevere insulitisSpontaneous diabetes
2020
Crosstalk between circadian rhythms and the microbiota
Pearson JA, Wong FS, Wen L. Crosstalk between circadian rhythms and the microbiota. Immunology 2020, 161: 278-290. PMID: 33090484, PMCID: PMC7692254, DOI: 10.1111/imm.13278.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCircadian ClocksCircadian RhythmDisease SusceptibilityDysbiosisGastrointestinal MicrobiomeHumansImmune SystemImmunityReceptors, Pattern RecognitionConceptsHost circadian rhythmsMicrobial oscillationsGene/protein expressionAspects of biologyCircadian rhythmMicrobial associationsMolecular oscillationsCircadian oscillationsMicrobial compositionMicrobial influenceCause diseaseMolecular techniquesHost metabolismDisease susceptibilityMicrobial changesProtein expressionPeripheral rhythmsMain inducerMicrobiotaSleep-wake cycleHost immunityCrosstalkClinical successPotential connectionMicrobesAltered Systemic and Intestinal IgA Immune Responses in Individuals With Type 1 Diabetes
Huang J, Huang G, Li X, Hu F, Xie Z, Xiao Y, Luo S, Chao C, Guo K, Wong FS, Zhou Z, Wen L. Altered Systemic and Intestinal IgA Immune Responses in Individuals With Type 1 Diabetes. The Journal Of Clinical Endocrinology & Metabolism 2020, 105: dgaa590. PMID: 32860693, PMCID: PMC7549925, DOI: 10.1210/clinem/dgaa590.Peer-Reviewed Original ResearchConceptsIgA-bound bacteriaType 1 diabetesHealthy control individualsIgA immune responseControl individualsIgA immunityAutoantibody titersIgA concentrationsImmune responseType 1 diabetes patientsΒ-cell autoimmunityLonger disease durationSerum IgA concentrationNovel therapeutic targetEnzyme-linked immunosorbentDisease durationIgA levelsDiabetes patientsDiabetes displayGut homeostasisBlood samplesOral cavityTherapeutic targetDiabetesHost immunityGut microbial metabolites alter IgA immunity in type 1 diabetes
Huang J, Pearson JA, Peng J, Hu Y, Sha S, Xing Y, Huang G, Li X, Hu F, Xie Z, Xiao Y, Luo S, Chao C, Wong F, Zhou Z, Wen L. Gut microbial metabolites alter IgA immunity in type 1 diabetes. JCI Insight 2020, 5 PMID: 32298241, PMCID: PMC7259536, DOI: 10.1172/jci.insight.135718.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAnimalsChildDiabetes Mellitus, Type 1Fatty Acids, VolatileFemaleGastrointestinal MicrobiomeHumansImmunoglobulin AMaleMiceMice, Inbred NODConceptsType 1 diabetesGut microbiotaNOD miceImmune responseGerm-free NOD miceIgA immune responseIgA-mediated immunityHealthy control subjectsPotential therapeutic agentShort-chain fatty acid productionIgA immunityT1D preventionIgA responsesControl subjectsDecreased severityT1DTherapeutic agentsFunctional effectsMicrobiotaDiabetesPatientsUnderlying mechanismMiceImmunitySCFA
2019
Norovirus Changes Susceptibility to Type 1 Diabetes by Altering Intestinal Microbiota and Immune Cell Functions
Pearson JA, Tai N, Ekanayake-Alper DK, Peng J, Hu Y, Hager K, Compton S, Wong FS, Smith PC, Wen L. Norovirus Changes Susceptibility to Type 1 Diabetes by Altering Intestinal Microbiota and Immune Cell Functions. Frontiers In Immunology 2019, 10: 2654. PMID: 31798584, PMCID: PMC6863139, DOI: 10.3389/fimmu.2019.02654.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaliciviridae InfectionsDiabetes Mellitus, Type 1Disease SusceptibilityGastrointestinal MicrobiomeMiceMice, Inbred NODNorovirusT-LymphocytesConceptsExpansion of TregsNOD miceT cellsMNV4 infectionMucosal immunityNon-obese diabetic (NOD) mouse modelGerm-free NOD miceFirmicutes/Bacteroidetes ratioProinflammatory T cellsRole of norovirusesTuft cell markersDevelopment of T1DInflammatory T cellsCommon enteric virusesB cell subsetsDiabetic mouse modelImmune cell functionType 1 diabetes susceptibilityEnteric virusesNaïve splenocytesT1D protectionTreg numbersImmunological changesMucosal antibodiesT1D development
2016
Different immunological responses to early-life antibiotic exposure affecting autoimmune diabetes development in NOD mice
Hu Y, Jin P, Peng J, Zhang X, Wong FS, Wen L. Different immunological responses to early-life antibiotic exposure affecting autoimmune diabetes development in NOD mice. Journal Of Autoimmunity 2016, 72: 47-56. PMID: 27178773, PMCID: PMC4958594, DOI: 10.1016/j.jaut.2016.05.001.Peer-Reviewed Original ResearchConceptsAntigen-presenting cellsType 1 diabetesAutoimmune diabetes developmentDiabetes developmentPregnant mothersEarly-life antibiotic exposureTolerogenic antigen-presenting cellsUntreated control miceInflammatory T cellsDifferent immunological responsesGut microbiota compositionDifferent immune responsesImportant environmental agentsGut bacterial compositionEarly time pointsNOD miceControl miceAutoimmune diseasesPrenatal exposureLymphoid organsAntibiotic exposureT cellsImmune responseImmunological responseNew therapiesThe Gut Microbiome in the NOD Mouse
Peng J, Hu Y, Wong FS, Wen L. The Gut Microbiome in the NOD Mouse. Methods In Molecular Biology 2016, 1433: 169-177. PMID: 27032947, DOI: 10.1007/7651_2016_331.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNA, BacterialFecesGastrointestinal MicrobiomeGenetic MarkersIntestinal MucosaIntestinesMiceMice, Inbred NODRNA, Ribosomal, 16SSequence Analysis, DNAConceptsType 1 diabetes developmentNOD miceDiabetes developmentGut bacteriaSusceptible NOD miceNonobese diabetic (NOD) miceBacterial DNA sequencingGut microbiome compositionGut microbiome analysisMouse fecal samplesExcellent mouse modelDiabetic miceMouse modelGut microbiotaGut microbiomeIntestinal contentsMiceCritical modulatorDisease phenotypeFecal samplesMicrobiome compositionStandard protocolMicrobiome analysisHealthPathogenic microorganisms